Dietary effects of the inclusion of white lupine seeds and different types of binders on the blood indicators of young Dwarf Lop rabbits V, Kudelkova L, Strakova E, Suchy P, Zapletal D (2018): Dietary effects of the inclusion of white lupine seeds and different types of binders on the blood indicators of young Dwarf Lop rabbits. Veterinarni Medicina, 63: 379-389.
download PDF

The aim of the present study was to evaluate the effects of the inclusion of whole seeds of white lupine and different binder types in complete pelleted diets on selected haematological and plasma biochemical indicators of growing dwarf rabbits. The individual litters of Dwarf Lop rabbit kits were randomised after birth into control (n = 12) and experimental (E) groups. The young rabbits of the E group (n = 24) received an experimental pre-weaning diet containing a 25% share of white lupine seeds. At the time of weaning, the live weights of eight-week-old rabbits in the control group and E group were 727 g and 743 g, respectively. The weaned rabbits of the E group were further equally divided into two post-weaning experimental dietary groups (M = 12; G = 12); these diets contained a 20% share of white lupine seeds each and their composition only differed in the type of the binder (molasses or glycerol component). The rabbits of the control group received a commercial pet rabbit diet with no white lupine seeds during the entire experimental period. At the age of eight weeks, the diet showed a significant effect on the level of albumin (P < 0.05), A/G ratio (P < 0.05) and ALP activity (< 0.01) in plasma of dwarf rabbit kits. Significant effects of diet were found in 15-week old rabbits in relation to the plasma level of albumin (P < 0.05), Ca (P < 0.05), A/G ratio (P < 0.05) and ALT activity (< 0.01). The dietary inclusion of white lupine seeds resulted in beneficial health effects on certain blood indicators of rabbits, and their use in the proportion of 25% in the pre-weaning diet and 20% in the post-weaning diet can be recommended as a suitable feed additive for dwarf rabbits. The use of crude glycerol as binder in the experimental diet had no adverse effect on the blood indicators of the growing rabbits and thus it can be recommended for the manufacture of feed pellets intended for dwarf rabbits.

Al-harbi MS, Al-Bashan MM, Amrah KAFA (2014): Impacts of feeding with Lupinus albus (White Lupin) and Lupinus termis (Egyptian Lupin) on physiological activities and histological structures of some rabbit’s organs, at Taif Governorate. World Journal of Zoology 9, 166–177.
Bähr Melanie, Fechner Anita, Kiehntopf Michael, Jahreis Gerhard (2015): Consuming a mixed diet enriched with lupin protein beneficially affects plasma lipids in hypercholesterolemic subjects: A randomized controlled trial. Clinical Nutrition, 34, 7-14
Bamikole MA, Enzenwa I, Adewumi MK, Omojola AB, Aken’ova ME, Babayemi OJ, Olufosoye OF (2000): Alternative feed resources for formulating concentrate diets of rabbits. 2. Jack beans (Canavalia ensiformis) seeds. World Rabbit Science 8, 131–136.
Banfi G (2006): Relation between serum creatinine and body mass index in elite athletes of different sport disciplines * Commentary. British Journal of Sports Medicine, 40, 675-678
Bouchoucha R, Fradj MKB, Bouchoucha M, Akrout M, Feki M, Kaabachi N, Raies A, Slimane H (2016): Effect of on Lupinus Albus glycaemic control, plasma insulin levels, lipid profile and liver enzymes in type 2 diabetics. Journal of Food and Nutrition Research 4, 615–620.
Campbell TW (2012): Clinical chemistry of mammals: Laboratory animals and miscellaneous species. In: Thrall MA, Weiser G, Allison RW, Campbell TW (eds): Veterinary Hematology and Clinical Chemistry. 2nd edn. Wiley-Blackwell, Ames. 571–581.
Čonková Eva, Laciaková Anna, Pástorová Bernadeta, Seidel Herbert, Kováč Gabriel (2001): The effect of zearalenone on some enzymatic parameters in rabbits. Toxicology Letters, 121, 145-149
Donkin S.S., Koser S.L., White H.M., Doane P.H., Cecava M.J. (2009): Feeding value of glycerol as a replacement for corn grain in rations fed to lactating dairy cows. Journal of Dairy Science, 92, 5111-5119
Eckersall PD (2008): Proteins, proteomics, and the dysproteinemias. In: Kaneko JJ, Harvey JW, Bruss ML (eds): Clinical Biochemistry of Domestic Animals. 6th edn. Elsevier Academic Press, California. 117–155.
Fontanari Gustavo Guadagnucci, Batistuti José Paschoal, Cruz Robison José da, Saldiva Paulo Hilário Nascimento, Arêas José Alfredo Gomes (2012): Cholesterol-lowering effect of whole lupin (Lupinus albus) seed and its protein isolate. Food Chemistry, 132, 1521-1526
Gillett CS (1994): Selected drug dosages and clinical reference data. In: Manning PJ, Ringler DH, Newcomer CE (eds): The Biology of the Laboratory Rabbit. 2nd edn. Academic Press. 467–472.
González-Redondo Pedro, Contreras-Chacón Gema M. (2012): Perceptions among university students in Seville (Spain) of the rabbit as livestock and as a companion animal. World Rabbit Science, 20, -
Harcourt-Brown F (2002): Textbook of rabbit medicine. In: Harcourt-Brown F (ed.): Clinical pathology. 1st edn. Butterworth-Heinemann, Oxford. 140–165.
Harcourt-Brown F. M., Harcourt-Brown S.F. (2012): Clinical value of blood glucose measurement in pet rabbits. Veterinary Record, 170, 674-674
Harisa Gamaleldin I., Alanazi Fars K. (2015): The beneficial roles of Lupineus luteus and lifestyle changes in management of metabolic syndrome: A case study. Saudi Pharmaceutical Journal, 23, 712-715
Iñigo M.A., Blas J.C. de, Cachaldora P., García-Rebollar P. (2011): EFFECT OF STARCH SUBSTITUTION WITH CRUDE GLYCEROL ON GROWING RABBIT AND LACTATING DOE PERFORMANCE. World Rabbit Science, 19, 67-74
Jeklova E., Leva L., Knotigova P., Faldyna M. (2009): Age-related changes in selected haematology parameters in rabbits. Research in Veterinary Science, 86, 525-528
Kroupa Leo, Suchý Pavel, Straková Eva, Herzig Ivan (2011): Glycerol as source of energy in broiler chicken fattening. Acta Veterinaria Brno, 80, 157-164
Kudělková Lenka, Pavlata Leoš, Pechová Alena, Filípek Jaroslav (2016): Blood serum protein in periparturient goats supplemented with various forms of zinc. Acta Veterinaria Brno, 85, 387-394
Lowe JA (2010): Pet rabbit feeding and nutrition. In: de Blas C, Wiseman J (eds): Nutrition of the Rabbit. 2nd edn. CAB-International, Wallingford. 294–313.
Marchesi Marta, Parolini Cinzia, Diani Erika, Rigamonti Elena, Cornelli Lorena, Arnoldi Anna, Sirtori Cesare R., Chiesa Giulia (2008): Hypolipidaemic and anti-atherosclerotic effects of lupin proteins in a rabbit model. British Journal of Nutrition, 100, -
Martins José M., Riottot Michel, de Abreu Manuel C., Viegas-Crespo Ana M., Lança Maria J., Almeida José A., Freire João B., Bento Ofélia P. (2005): Cholesterol-lowering effects of dietary blue lupin ( Lupinus angustifolius L.) in intact and ileorectal anastomosed pigs. Journal of Lipid Research, 46, 1539-1547
Meredith A (2012): Is obesity a problem in pet rabbits? Veterinary Record 171, 192–193.
Meuten D (2012): Laboratory evaluation and interpretation of the urinary system. In: Thrall MA, Weiser G, Allison RW, Campbell TW (eds): Veterinary Hematology and Clinical Chemistry. 2nd edn. Wiley-Blackwell, Ames. 323–377.
Okoye CN, Ochiogu IS, Onah CE (2016): The effects of monosodium l-glutamate administration on the reproduction and serum biochemistry of adult male rabbits. Veterinární Medicína, 61, 141-147
Osman M., Mahmoud G. I., Romeilah R. M., Fayed S. A. (2011): Lupin seeds lower plasma lipid concentrations and normalize antioxidant parameters in rats. Grasas y Aceites, 62, 162-170
Prebble JL (2014): Nutrition and feeding. In: Meredith A, Lord B (eds): BSAVA Manual of Rabbit Medicine. 1st edn. BSAVA, Gloucester. 27–35.
Prebble J. L., Meredith A. L. (2014): Food and water intake and selective feeding in rabbits on four feeding regimes. Journal of Animal Physiology and Animal Nutrition, 98, 991-1000
Rupič V., Škrlin J., Mužic S., Šerman V., Stipić N., Bačar-Huskić L. (1999): Protein and Fat Concentrations in the Blood Serum of Rabbits Fed Different Quantities of Dried Olive Cake. Acta Veterinaria Brno, 68, 91-98
Schroder A, Sudekum KH (1999): Glycerol as a by-product of biodiesel production in diets for ruminants. In: Proceedings of the 10th International Rapeseed Congress, Canberra, Australia. Available at
Simek V, Zapletal D, Strakova E, Pavlik A, Suchy P (2017a): Physiological values of some blood indicators in selected dwarf rabbit breeds. World Rabbit Science 25, 27–36.
Simek V, Zapletal D, Kudelkova L, Jakesova P, Strakova E, Suchy P (2017b): Selected blood biochemical indicators of young Dwarf Lop rabbit females in relation to the different diets. In: Proceedings of International Animal Nutrition PhD Conference, Ceske Budejovice, Czech Republic. 121–127.
Sirtori Cesare R., Lovati Maria Rosa, Manzoni Cristina, Castiglioni Silvia, Duranti Marcello, Magni Chiara, Morandi Sheila, D'Agostina Alessandra, Arnoldi Anna (2004): Proteins of White Lupin Seed, a Naturally Isoflavone-Poor Legume, Reduce Cholesterolemia in Rats and Increase LDL Receptor Activity in HepG2 Cells. The Journal of Nutrition, 134, 18-23
Stanek Maria, Rotkiewicz Tadeusz, Sobotka Wiesław, Bogusz Jacek, Otrocka-Domagała Iwona, Rotkiewicz Agnieszka (2015): The effect of alkaloids present in blue lupine (Lupinus angustifolius) seeds on the growth rate, selected biochemical blood indicators and histopathological changes in the liver of rats. Acta Veterinaria Brno, 84, 55-62
Straková E., Suchý P., Večerek V., Šerman V., Mas N., Jůzl M. (2006): Nutritional Composition of Seeds of the Genus Lupinus. Acta Veterinaria Brno, 75, 489-493
Tothova C., Nagy O., Kovac G. (2016): Serum proteins and their diagnostic utility in veterinary medicine: a review. Veterinární Medicína, 61, 475-496
Vavricka Stephan R., Burri Emanuel, Beglinger Christoph, Degen Lukas, Manz Michael (2009): Serum Protein Electrophoresis: An Underused but Very Useful Test. Digestion, 79, 203-210
Vennen KM, Mitchell MA (2009): Rabbits. In: Mitchell MA, Tully jr TN (eds): Manual of Exotic Pet Practice. 1st edn. Saunders Elsevier, St Louis. 375–404.
Villamide M.J., Carabaño R., Maertens L., Pascual J., Gidenne T., Falcao-E-Cunha L., Xiccato G. (2009): Prediction of the nutritional value of European compound feeds for rabbits by chemical components and in vitro analysis. Animal Feed Science and Technology, 150, 283-294
Viveros A., Centeno C., Arija I., Brenes A. (2007): Cholesterol-Lowering Effects of Dietary Lupin (Lupinus albus var Multolupa) in Chicken Diets. Poultry Science, 86, 2631-2638
Volek Z., Marounek M. (2009): Whole white lupin (Lupinus albus cv. Amiga) seeds as a source of protein for growing-fattening rabbits. Animal Feed Science and Technology, 152, 322-329
Volek Z., Marounek M., Volková L., Kudrnová E. (2014): Effect of diets containing whole white lupin seeds on rabbit doe milk yield and milk fatty acid composition as well as the growth and health of their litters1. Journal of Animal Science, 92, 2041-2049
Wesche P (2014): Clinical pathology. In: Meredith A, Lord B (eds): BSAVA Rabbit Medicine. 1st edn. British Small Animal Veterinary Association, Gloucester. 124–137.
Zhang Xiao-jun, Chinkes David L., Aarsland Asle, Herndon David N., Wolfe Robert R. (2008): Lipid Metabolism in Diet-Induced Obese Rabbits Is Similar to That of Obese Humans. The Journal of Nutrition, 138, 515-518
download PDF

© 2018 Czech Academy of Agricultural Sciences