Acute toxicity of two pyrethroid insecticides for five non-indigenous crayfish species in Europe J., Buric M., Kouba A., Velisek J. (2019): Acute toxicity of two pyrethroid insecticides for five non-indigenous crayfish species in Europe. Veterinarni Medicina, 64: 125-133.
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Pyrethroid insecticides are highly toxic to many aquatic organisms. The aim of this study was to evaluate the toxicity of the commercial products Cyperkill 25 EC (active compound 250 g/l cypermethrin) and Decis Mega (active compound 50 g/l deltamethrin) for European non-indigenous marbled crayfish Procambarus virginalis, red swamp crayfish Procambarus clarkii, signal crayfish Pacifastacus leniusculus, spiny-cheek crayfish Orconectes limosus and yabby Cherax destructor. These data will provide a baseline for potential programmes to eradicate alien crayfish from Europe (EU Regulation No. 1143/2014; Commission Implementing Regulation No. 2016/1141) and are also relevant globally. The 96hLC50 values of Cyperkill 25 EC were 0.09, 0.17, 0.18, 0.19 and 0.30 µg/l for spiny-cheek crayfish, red swamp crayfish, marbled crayfish, signal crayfish and yabby, respectively. In the same order, the 96hLC50 values of Decis Mega were 0.76, 0.16, 0.21, 0.03 and 0.27 µg/l. The toxicity of the insecticides was similar and species-specific, possibly reflecting the size difference of the tested animals. This study shows that cypermethrin and deltamethrin are highly toxic to the tested crayfish species at low concentrations. This high sensitivity, along with the low accumulation in the food chain and short-term persistence in the aquatic environment, suggests that they are suitable biocides for eradicating crayfish in the wild. Stagnant, closed water bodies with newly emerging invaders are ideal sites for possible application, although local conditions must be considered.

Adam Olivier, Degiorgi François, Crini Grégorio, Badot Pierre-Marie (2010): High sensitivity of Gammarus sp. juveniles to deltamethrin: Outcomes for risk assessment. Ecotoxicology and Environmental Safety, 73, 1402-1407
Amweg Erin L., Weston Donald P., Ureda Nicole M. (2005): USE AND TOXICITY OF PYRETHROID PESTICIDES IN THE CENTRAL VALLEY, CALIFORNIA, USA. Environmental Toxicology and Chemistry, 24, 966-
Beketov M. A. (2004): Comparative Sensitivity to the Insecticides Deltamethrin and Esfenvalerate of Some Aquatic Insect Larvae (Ephemeroptera and Odonata) and Daphnia magna. Russian Journal of Ecology, 35, 200-204
Booy Olaf, Mill Aileen C., Roy Helen E., Hiley Alice, Moore Niall, Robertson Pete, Baker Simon, Brazier Matt, Bue Mathilde, Bullock Richard, Campbell Steve, Eyre Dominic, Foster Jim, Hatton-Ellis Maggie, Long Jo, Macadam Craig, Morrison-Bell Camilla, Mumford John, Newman Jonathan, Parrott David, Payne Robin, Renals Trevor, Rodgers Eoina, Spencer Mark, Stebbing Paul, Sutton-Croft Mike, Walker Kevin J., Ward Alastair, Whittaker Stan, Wyn Gabe (2017): Risk management to prioritise the eradication of new and emerging invasive non-native species. Biological Invasions, 19, 2401-2417
Breckenridge Charles B., Holden Larry, Sturgess Nicholas, Weiner Myra, Sheets Larry, Sargent Dana, Soderlund David M., Choi Jin-Sung, Symington Steve, Clark J. Marshall, Burr Steve, Ray David (2009): Evidence for a separate mechanism of toxicity for the Type I and the Type II pyrethroid insecticides. NeuroToxicology, 30, S17-S31
Buřič Miloš, Hulák Martin, Kouba Antonín, Petrusek Adam, Kozák Pavel, Etges William J. (2011): A Successful Crayfish Invader Is Capable of Facultative Parthenogenesis: A Novel Reproductive Mode in Decapod Crustaceans. PLoS ONE, 6, e20281-
Cecchinelli Elena, Aquiloni Laura, Maltagliati Giacomo, Orioli Gabriele, Tricarico Elena, Gherardi Francesca (2012): Use of natural pyrethrum to control the red swamp crayfish Procambarus clarkii in a rural district of Italy. Pest Management Science, 68, 839-844
CHMI – Czech Hydrometeorological Institute (2018): On-line water duality database. Available at (Accessed December 2, 2018).
Collins P., Cappello S. (2006): Cypermethrin Toxicity to Aquatic Life: Bioassays for the Freshwater Prawn Palaemonetes argentinus. Archives of Environmental Contamination and Toxicology, 51, 79-85
Commission Implementing Regulation (EU) 2016/1141 of 13 July 2016 adopting a list of invasive alien species of Union concern pursuant to Regulation (EU) No. 1143/2014 of the European Parliament and of the Council.
DeLorenzo Marie E., Key Peter B., Chung Katy W., Sapozhnikova Yelena, Fulton Michael H. (2014): Comparative toxicity of pyrethroid insecticides to two estuarine crustacean species, Americamysis bahia and Palaemonetes pugio. Environmental Toxicology, 29, 1099-1106
Elliott M, Farnham AW, Janes NF, Needham PH, Pulman DA (1974): Insecticidally active conformations of pyrethroids. In: Kohn GK (ed.): Mechanism of Pesticide Action. American Chemical Society, Washington, DC. 80–91.
Erstfeld Karen M. (1999): Environmental fate of synthetic pyrethroids during spray drift and field runoff treatments in aquatic microcosms. Chemosphere, 39, 1737-1769
Gherardi F, Aquiloni L, Dieguez-Uribeondo J, Tricarico E (2011): Managing invasive crayfish: is there a hope? Aquatic Science 73, 185–200.
Golow AA, Godzi TA (1994): Acute toxicity of deltamethrin and dieldrin to Oreochromis niloticus (L.). Bulletin of Environmental Contamination and Toxicology 52, 351–354.
Hart J. L., Thacker J. R. M., Braidwood J. C., Fraser N. R., Matthews J. E. (1997): Novel cypermethrin formulation for the control of sea lice on salmon (Salmo salar). Veterinary Record, 140, 179-181
Haya Katsuji (1989): Toxicity of pyrethroid insecticides to fish. Environmental Toxicology and Chemistry, 8, 381-391
Haya K, Burridge LE, Davies IM, Ervik A (2005): A review and assessment of environmental risk of chemicals used for the treatment of sea lice infestations of cultured salmon. In: Hargrave B (ed.): The Handbook of Environmental Chemistry. Environmental Effects of Marine Finfish Aquaculture. Springer-Verlag. 305–340.
Holdich D.M., Reynolds J.D., Souty-Grosset C., Sibley P.J. (2009): A review of the ever increasing threat to European crayfish from non-indigenous crayfish species. Knowledge and Management of Aquatic Ecosystems, , 11-
Kocourek V, Hajslova J (1989): Methods for determination of contaminants in food: Laboratory Manual I. (in Czech). Stredisko technickych informaci potravinarskeho prumyslu Praha, 9−15. ISBN 80-85120-00-3.
Kouba A., Petrusek A., Kozák P. (2014): Continental-wide distribution of crayfish species in Europe: update and maps. Knowledge and Management of Aquatic Ecosystems, , 05-
Kouba Antonín, Tíkal Jan, Císař Petr, Veselý Lukáš, Fořt Martin, Příborský Josef, Patoka Jiří, Buřič Miloš (2016): The significance of droughts for hyporheic dwellers: evidence from freshwater crayfish. Scientific Reports, 6, -
Kozák P., Buřič M., Kanta J., Kouba A., Hamr P., Policar T. (2009): The effect of water temperature on the number of moults and growth of juvenile signal crayfish <I>Pacifastacus leniusculus</I> Dana. Czech Journal of Animal Science, 54, 286-292
Kumar Amit, Sharma Bechan, Pandey Ravi Shankar (2007): Preliminary Evaluation of The Acute Toxicity of Cypermethrin and λ-Cyhalothrin to Channa Punctatus. Bulletin of Environmental Contamination and Toxicology, 79, 613-616
Li Huizhen, Cheng Fei, Wei Yanli, Lydy Michael J., You Jing (2017): Global occurrence of pyrethroid insecticides in sediment and the associated toxicological effects on benthic invertebrates: An overview. Journal of Hazardous Materials, 324, 258-271
Liu Weiping, Gan Jay J., Lee Sangjin, Kabashima John N. (2004): PHASE DISTRIBUTION OF SYNTHETIC PYRETHROIDS IN RUNOFF AND STREAM WATER. Environmental Toxicology and Chemistry, 23, 7-
Lodge David M., Taylor Christopher A., Holdich David M., Skurdal Jostein (2000): Nonindigenous Crayfishes Threaten North American Freshwater Biodiversity: Lessons from Europe. Fisheries, 25, 7-20<0007:NCTNAF>2.0.CO;2
Marigoudar SR, Ahmed RN, David M (2009): Cypermethrin induced respiratory and behavioural responses of the freshwater teleost, Labeo rohita (Hamilton). Veterinarski Arhiv 79, 583–590.
Marino D., Ronco A. (2005): Cypermethrin and Chlorpyrifos Concentration Levels in Surface Water Bodies of the Pampa Ondulada, Argentina. Bulletin of Environmental Contamination and Toxicology, 75, 820-826
Mian LS, Mulla MS (1992): Effects of pyrethroid insecticides on nontarget invertebrates in aquatic ecosystems. Journal of Agricultural Entomology 9, 73–98.
Moid N, Patel F, Shrimali A, Desai KR, Highland HN (2012): Toxicological implications of Type II pyrethroides, Deltamethrin on certain tissue of swiss albino male mice. International Journal of Pharma Research and Health Sciences 5, 41–54.
Muir Derek C. G., Rawn Gary P., Grift Norbert P. (1985): Fate of the pyrethroid insecticide deltamethrin in small ponds: a mass balance study. Journal of Agricultural and Food Chemistry, 33, 603-609
OECD − Organisation for Economic Co-operation and Development (1992): Guidelines for the Testing of Chemicals Test No. 203: Fish, Acute Toxicity Test. 12 pp.
Palmquist K, Fairbrother A, Salatas J (2012): Pyrethroid insecticides: use, environmental fate, and ecotoxicology. In: Perveen F (ed.): Advances in Integrated Pest Management. InTech. 1–70.
Patoka Jiří, Buřič Miloš, Kolář Vojtěch, Bláha Martin, Petrtýl Miloslav, Franta Pavel, Tropek Robert, Kalous Lukáš, Petrusek Adam, Kouba Antonín (2016): Predictions of marbled crayfish establishment in conurbations fulfilled: Evidences from the Czech Republic. Biologia, 71, -
Peay S., Dunn A.M. (2014): The behavioural response of the invasive signal crayfish Pacifastacus leniusculus to experimental dewatering of burrows and its implications for eradication treatment and management of ponds with crayfish. Ethology Ecology & Evolution, 26, 277-298
PEAY S., HILEY P. D., COLLEN P., MARTIN I. (2006): BIOCIDE TREATMENT OF PONDS IN SCOTLAND TO ERADICATE SIGNAL CRAYFISH. Bulletin Français de la Pêche et de la Pisciculture, , 1363-1379
Regulation (EU) No. 1143/2014 of the European Parliament and of the Council of 22 October 2014 on the prevention and management of the introduction and spread of invasive alien species.
Saha Suchismita, Kaviraj Anilava (2008): Acute Toxicity of Synthetic Pyrethroid Cypermethrin to Some Freshwater Organisms. Bulletin of Environmental Contamination and Toxicology, 80, 49-52
Sandodden Roar, Johnsen Stein Ivar (2010): Eradication of introduced signal crayfish Pasifastacus leniusculus using the pharmaceutical BETAMAX VET.®. Aquatic Invasions, 5, 75-81
Scholtz Gerhard, Braband Anke, Tolley Laura, Reimann André, Mittmann Beate, Lukhaup Chris, Steuerwald Frank, Vogt Günter (2003): Parthenogenesis in an outsider crayfish. Nature, 421, 806-806
Smith TM, Stratton GW (1986): Effects of synthetic pyrethroid insecticides on nontarget organisms. Residue Reviews 97, 93–120.
Stebbing P., Longshaw M., Scott A. (2014): Review of methods for the management of non-indigenous crayfish, with particular reference to Great Britain. Ethology Ecology & Evolution, 26, 204-231
Stephenson R.R., Choi S.Y., Olmos-Jerez A. (1984): Determining the toxicity and hazard to fish of a rice insecticide. Crop Protection, 3, 151-165
Terech-Majewska Elżbieta, Schulz Patrycja, Kaczorek Edyta, Siwicki Andrzej K, Szarek Józef, Skibniewska Krystyna (2016): Non-specific cellular defence mechanisms of rainbow trout ( Oncorhynchus mykiss ) in intensive and extensive rearing technologies. Aquaculture Research, 47, 3585-3592
Tilman D., Balzer C., Hill J., Befort B. L. (2011): Global food demand and the sustainable intensification of agriculture. Proceedings of the National Academy of Sciences, 108, 20260-20264
Treasurer James W, Wadsworth Simon L (2004): Interspecific comparison of experimental and natural routes of Lepeophtheirus salmonis and Caligus elongatus challenge and consequences for distribution of chalimus on salmonids and therapeutant screening. Aquaculture Research, 35, 773-783
Velisek J., Wlasow T., Gomulka P., Svobodova Z., Dobsikova R., Novotny L., Dudzik M. (2012): Effects of cypermethrin on rainbow trout (Oncorhynchus mykiss)&nbsp;. Veterinární Medicína, 51, 469-476
Velisek J., Svobodova Z., Piackova V. (2009): Effects of acute exposure to bifenthrin on some haematological, biochemical and histopathological parameters of rainbow trout (<I>Oncorhynchus mykiss</I>). Veterinární Medicína, 54, 131-137
Velisek J, Stara A, Svobodova Z (2011): The effects of pyrethroid and triazine pesticides on fish physiology. In: Stoytcheva M (ed.): Pesticides in the Modern World: Pests Control and Pesticides Exposure and Toxicity Assessment. InTech. 377–402.
Velisek J, Kouba A, Stara A (2013): Acute toxicity of triazine pesticides to juvenile signal crayfish (Pacifastacus leniusculus). Neuroendocrinology Letters 34, 31–36.
Viran Rukiye, Ünlü Erkoç Figen, Polat Hilal, Koçak Oner (2003): Investigation of acute toxicity of deltamethrin on guppies (Poecilia reticulata). Ecotoxicology and Environmental Safety, 55, 82-85
Werner I, Moran K (2008): Effects of pyrethroid insecticides on aquatic organisms. In: Gan J, Spurlock F, Hendley P, Weston D (eds): Synthetic Pyrethroids: Occurrence and Behaviour in Aquatic Environments. American Chemical Society. 310–334.
WHO – World Health Organisation (1989): Cypermethrin – Health and Safety Guide. Geneva No. 22. 107 pp.
Wolansky M.J., Harrill J.A. (2008): Neurobehavioral toxicology of pyrethroid insecticides in adult animals: A critical review. Neurotoxicology and Teratology, 30, 55-78
Wu N., Wei H., Shen H., Guo M., Wu T. T. (2012): Acute toxic effects of deltamethrin on red swamp crayfish, Procambarus clarkii (Decapoda, Cambaridae). Crustaceana, 85, 993-1005
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