Cisternal versus lumbar cerebrospinal fluid lactate concentration in healthy dogs A., Seisdedos-Benzal A., Carletti B., Quiros S., Martin E., Menor D., Granados M. (2020): Cisternal versus lumbar cerebrospinal fluid lactate concentration in healthy dogs. Veterinarni Medicina, 65: 297-300.
download PDF

The analysis of cerebrospinal fluid biomarkers provides a wide range of information about the neurological health of a patient. Lactate is a metabolic precursor necessary for brain gluconeogenesis. When oxidative impairment or mitochondrial damage is present, lactate alteration occurs. The rostro-caudal dynamics of cerebrospinal biomarkers along the craniospinal axis in humans and horses was demonstrated in other studies. To the authors’ knowledge, no clinical study has, so far, investigated the cerebrospinal fluid lactate concentration in dogs in association with the puncture site. The purpose of this study was to compare the cerebellomedullary cistern and lumbar cistern cerebrospinal fluid lactate concentrations in healthy dogs. Cerebellomedullary and lumbar cerebrospinal fluids were collected for the cell count, total protein determination and lactate analysis from ten healthy Beagle dogs. The results revealed a significantly increased lumbar cerebrospinal fluid lactate concentration when compared with the cerebellomedullary cistern level. The results included: the total nucleated cell count < 5 cells/µl, the red blood cell count < 500 cell/µl, the total proteins < 0.3 g/l, as well as the cerebellomedullary lactate values (1.44 ± 0.06 mM/l) and the lumbar cistern lactate values (1.58 ± 0.1 mM/l). The results of this study highlight useful data that help to understand the physiological lactate variations depending on the cerebrospinal fluid puncture site.

Andrews FM, Matthews HK, Reed SM. The ancillary techniques and test for diagnosing equine neurological disease. Vet Med. 1990;85(12):1325-30.
Caines D, Sinclair M, Wood D, Valverde A, Dyson D, Gaitero L, Nykamp S. Evaluation of cerebrospinal fluid lactate and plasma lactate concentrations in anesthetized dogs with and without intracranial disease. Can J Vet Res. 2013;77(4):297-302.
Di Terlizzi R, Platt S. The function, composition and analysis of cerebrospinal fluid in companion animals: Part II-Analysis. Vet J. 2009;180(1):15-32.
Horn T, Klein J. Lactate levels in the brain are elevated upon exposure to volatile anesthetics: A microdialysis study. Neurochem Int. 2010 Dec;57(8):940-7.
Il-Hwan K, Dong-In J, Jong-Hyun Y, Byeong-Teck K, Chul-Park P, Hee-Myung P. Cerebrospinal fluid analysis in 13 clinically healthy Beagle dogs; hematological, biochemical and electrophoretic findings. Korean J Vet Res. 2008;48:105-10.
Galan A, Carletti BE, Morgaz-Rodriguez J, Gamito Gomez A, Munoz-Rascon P, Marin-Suarez EM. Cerebrospinal fluid lactate and pyruvate concentration and their ratio in healthy adult dogs. Vet Rec. 2013 Jul;173(10):249-51.
Magistretti PJ, Pellerin L. Cellular mechanisms of brain energy metabolism and their relevance to functional brain imaging. Philos Trans R Soc Lond B Biol Sci. 2000 Jul 29;354(1387):1155-63.
Pang DS, Boysen S. Lactate in veterinary critical care: Pathophysiology and management. J Am Anim Hosp Assoc. 2007 Sep-Oct;43(5):270-9.
Pugliese M, Carrasco JL, Andrade C, Mas E, Mascort J, Mahy N. Severe cognitive impairment correlates with higher cerebrospinal fluid levels of lactate and pyruvate in a canine model of senile dementia. Prog Neuropsychopharmacol Biol Psychiatry. 2005 May;29(4):603-10.
Reiber H. Proteins in cerebrospinal fluid and blood: Barriers, CSF flow rate and source-related dynamics. Restor Neurol Neurosci. 2003;21(3-4):79-96.
Seisdedos A, Galan A, Carletti B, Quiros S, Funes F, Martin EM, Dominguez JM, Gomez R, Granados MM. Anesthetic effects of isoflurane and propofol on cerebrospinal fluid biochemical markers in healthy dogs. Vet Clin Pathol. 2019Jun;48(2):270-5.
Sugi T, Fujishima M, Omae T. Lactate and pyruvate concentrations, and acid-base balance of cerebrospinal fluid in experimentally induced intracerebral and subarachnoid hemorrhage in dogs. Stroke. 1975 Nov-Dec;6(6):715-9.
Tarnaris A, Toma AK, Chapman MD, Petzold A, Keir G, Kitchen ND, Watkins LD. Rostrocaudal dynamics of CSF biomarkers. Neurochem Res. 2011 Mar;36(3):528-32.
Vaughn DM, Coleman E, Simpson ST, Whitmer B, Satjawatcharaphong C. A rostrocaudal gradient for neurotransmitter metabolites and a caudorostral gradient for protein in canine cerebrospinal fluid. Am J Vet Res. 1988 Dec;49(12):2134-7.
Witsberger TH, Levine JM, Fosgate GT, Slater MR, Kerwin SC, Russell KE, Levine GJ. Associations between cerebrospinal fluid biomarkers and long-term neurologic outcome in dogs with acute intervertebral disk herniation. J Am Vet Med Assoc. 2012 Mar 1;240(5):555-62.
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti