Essential oil of Rosmarinus officinalis induces in vitro anthelmintic and anticoccidial effects against Haemonchus contortus and Eimeria spp. in small ruminants

https://doi.org/10.17221/139/2020-VETMEDCitation:

Aouadi M, Sebai E, Saratsis A, Kantzoura V, Saratsi K, Msaada K, Sotiraki S, Akkari H (2021): Essential oil of Rosmarinus officinalis induces in vitro anthelmintic and anticoccidial effects against Haemonchus contortus and Eimeria spp. in small ruminants. Vet Med-Czech 66, 146–155.

download PDF

This work aimed to evaluate the valorisation of the volatile oil of “Rosmarinus officinalis L.”, a spontaneously growing medicinal plant in Tunisia, by studying its chemical composition, anthelmintic and anticoccidial potentials against Eimeria spp. and Haemonchus contortus at different essential oil concentrations. The main compounds of the R. officinalis essential oil identified by GC/MS were three monoterpenes: 1,8-cineole (52.06%), α-pinene (15.35%) and camphor (7.69%). The anticoccidial activity was estimated by the inhibition percentage of the oocyte sporulation in addition to the unsporulated and degenerated Eimeria oocysts using a haemocytometer after exposure to different essential oil concentrations. The essential oil was active against Eimeria spp. oocysts of sheep at IC50 = 1.82 ug/ml. Therefore, the IC50 values of the anticoccidial activity of this oil examined was 1.82 mg/ml. The anthelmintic efficacy of the rosemary volatile oil against Haemonchus contortus was realised by two in vitro tests: the egg hatch assay (EHA) and the adult worm’s motility assay (AWMA), by comparing this efficacy with albendazole (anthelmintic, of reference). In the egg hatch assay, the percentage of inhibition was observed at 16 mg/ml and was 73.76% after 2 days of incubation (IC50 = 11.41 mg/ml) and for the adult worm’s motility assay, it was 100% inhibition.

References:
Aboukhalid K, Lamiri A, Agacka-Moldoch M, Doroszewska T, Douaik A, Bakha M, Casanova J, Tomi F, Machon N, Al Faiz CA. Chemical polymorphism of Origanum compactum grown in all-natural habitats in Morocco. Chem Biodivers. 2016 Sep;13(9):1126-39. https://doi.org/10.1002/cbdv.201500511
 
Adams RP. Identification of essential oil components by gas chromatography/mass spectrometry. 4th ed. Carol Stream, IL: Allured Publishing Corporation; 2007. 811 p.
 
Akkari H, Jebali J, Gharbi M, Mhadhbi M, Awadi S, Darghouth MA. Epidemiological study of sympatric Haemonchus species and genetic characterization of Haemonchus contortus in domestic ruminants in Tunisia. Vet Parasitol. 2013 Mar;193(1-3):118-25. https://doi.org/10.1016/j.vetpar.2012.12.014
 
Akrout A, Hajlaoui H, Mighri H, Najjaa H, El Jani H, Zaidi S, Neffati M. Chemical and biological characteristics of essential oil of Rosmarinus officinalis cultivated in Djerba. J Essent Oil Bear Pl. 2010;13(4):398-411. https://doi.org/10.1080/0972060X.2010.10643841
 
Allen PC, Lydon J, Danforth HD. Effects of components of Artemisia annua on coccidia infections in chickens. Poult Sci. 1997 Aug;76(8):1156-63. https://doi.org/10.1093/ps/76.8.1156
 
Almela L, Sanchez-Munoz B, Fernandez-Lopez JA, Roca MJ, Rabe V. Liquid chromatographic–mass spectrometric analysis of phenolics and radical scavenging activity of rosemary extract from different raw material. J Chromatogr A. 2006 Jul;1120(1-2):221-9. https://doi.org/10.1016/j.chroma.2006.02.056
 
Alzieu JP, Mage C, Maes L, de Muelenaere C. Economic benefits of prophylaxis with diclazuril against subclinical coccidiosis in lambs reared indoors. Vet Rec. 1999 Apr 17;144(16):442-4. https://doi.org/10.1136/vr.144.16.442
 
Angioni A, Barra A, Cereti E, Barile D, Coisson JD, Arlorio M, Dessi S, Coroneo V, Cabras P. Chemical composition, plant genetic differences, antimicrobial and antifungal activity investigation of the essential of Rosmarinus officinalis L. J Agric Food Chem. 2004 Jun 2;52(11):3530-5. https://doi.org/10.1021/jf049913t
 
Assis LM, Bevilaqua CM, Morais SM, Vieira LS, Costa CT, Souza JA. Ovicidal and larvicidal activity in vitro of Spigelia anthelmia Linn. extracts on Haemonchus contortus. Vet Parasitol. 2003 Nov 3;117(1-2):43-9. https://doi.org/10.1016/j.vetpar.2003.07.021
 
Baratta MT, Dorma HJT, Deans SG, Figueiredo AC, Barroso JG, Ruberto G. Antimicrobial and antioxidant properties of some commercial essential oils. Flavour Frag J. 1998;13(4):235-44. https://doi.org/10.1002/(SICI)1099-1026(1998070)13:4<235::AID-FFJ733>3.0.CO;2-T
 
Benlarbi KH, Elmtili N, Macias FA, Galindo JCG. Influence of in vitro growth conditions in the production of defence compounds in Mentha pulegium L. Phytochem Lett. 2014 Apr;8:233-44. https://doi.org/10.1016/j.phytol.2014.03.007
 
Besier RB, Kahn LP, Sargison ND, Van Wyk JA. Diagnosis, treatment and management of Haemonchus contortus in small ruminants. Adv Parasitol. 2016;93:181-238.
 
Bicchi C, Binello A, Rubinol P. Determination of phenolic diterpene antioxidants in rosemary (Rosmarinus officinalis L.) with different methods of extraction and analysis. Phytochem Analysis. 2000 Jun;11(4):236-42. https://doi.org/10.1002/1099-1565(200007/08)11:4<236::AID-PCA503>3.0.CO;2-B
 
Boyom FF, Ngouana V, Zollo PHA, Menut C, Bessiere JM, Gut J, Rosenthal PJ. Composition and anti-plasmodial activities of essential oils from some Cameroonian medicinal plants. Phytochemistry. 2003 Dec;64(7):1269-75. https://doi.org/10.1016/j.phytochem.2003.08.004
 
Brenes A, Roura E. Essential oils in poultry nutrition: Main effects and modes of action. Anim Feed Sci Tech. 2010 Jun;158(1-2):1-14. https://doi.org/10.1016/j.anifeedsci.2010.03.007
 
Celiktas OY, Kocabas EEH, Bedir E, Vardar Sukan F, Ozek T, Baser KHC. Antimicrobial activities of methanol extracts and essential oils of Rosmarinus officinalis, depending on location and seasonal variations. Food Chem. 2007;100(2):553-9. https://doi.org/10.1016/j.foodchem.2005.10.011
 
Coles GC, Bauer C, Borgsteede FH, Geerts S, Klei TR, Taylor MA, Waller PJ. World association for the advancement of veterinary parasitology (W.A.A.V.P.) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Vet Parasitol. 1992 Sep;44(1-2):35-44. https://doi.org/10.1016/0304-4017(92)90141-U
 
Diaz-Maroto MC, Perez-Coello MS, Sanchez-Palomo E, Gonzalez-Vinas MA. Impact of drying and storage time on sensory characteristics of rosemary (Rosmarinus officinalis L.). J Sens Stud. 2007 Jan;22(1):34-48. https://doi.org/10.1111/j.1745-459X.2007.00093.x
 
Dittmar K, Mundt HC, Grzonka E, Daugschies A, Bangoura B. Ovine coccidiosis in housed lambs in Saxony-Anhalt (central Germany). Berl Munch Tierarztl Wochenschr. 2010 Jan-Feb;123(1-2):49-57.
 
do Amaral Franco J, da Rocha Afonsa ML, Rosmarinus L. In: Tutin TG, Heywood VH, Burges NA, Moore DM, Valentine DH, Walters SM, Webb DA, editors. Flora Europaea, Diapensiaceae to Myoporaceae, vol. 3. Cambridge: Cambridge University Press; 1972. 187 p.
 
Foreyt WJ. Coccidiosis and cryptosporidiosis in sheep and goats. Vet Clin North Am Food Anim Pract. 1990 Nov;6(3):655-70. https://doi.org/10.1016/S0749-0720(15)30838-0
 
Furnier G, Habib J, Ruguiguf A, Safta F, Guetari S. Study of different samples of the essential oil of Tunisian rosemary. Plants Med Phytother. 1983;23(3):180-5.
 
Gauly M, Reeg J, Bauer C, Erhardt G. Influence of production systems in lambs on the Eimeria oocyst output and weight gain. Small Ruminant Res. 2004 Oct;55(1-3):159-67. https://doi.org/10.1016/j.smallrumres.2004.02.001
 
Gregory MW, Joyner LP, Catchpole J, Norton CC. Ovine coccidiosis in England and Wales 1978–1979. Vet Rec. 1980 May 31;106(22):461-2. https://doi.org/10.1136/vr.106.22.461
 
Henriksen SA, Christensen JP. Demonstration of iso sporasuis oocysts in faecal samples. Vet Rec. 1992 Nov 7;131(19):443-4. https://doi.org/10.1136/vr.131.19.443
 
Hounzangbe-Adote MS, Paolini V, Fouraste I, Moutairou K, Hoste H. In vitro effects of four tropical plants on three life-cycle stages of the parasitic nematode, Haemonchus contortus. Res Vet Sci. 2005 Apr;78(2):155-60. https://doi.org/10.1016/j.rvsc.2004.05.009
 
Kennedy DG, Smyth WG, Hewitt SA, McEvoy JD. Monensin carry-over into unmedicated broiler feeds. Analyst. 1998 Dec;123(12):2529-33. https://doi.org/10.1039/a805197f
 
Kiarostami K, Bahrami M, Talebpour Z, Khanavi M, Hadjiakhoondi A. [Seasonal variation of Rosmarinus offi-cinalis L. essential oil]. J Med Pl. 2009;8(32):84-90. Persian.
 
Kirici S, Inan M. Effect of different harvesting time of the essential oil content of rosemary (Rosmarinus officinalis L) in the Cukurova conditions. In: Ozguven M, editor. Proceedings of the Workshop on Agricultural and Quality Aspects of Medicinal an Aromatic Plants. 2001 May;263-71.
 
Kirpik M. Evaluating qualitative rosemary (Rosmarinus officinalis L.) lines growing in arid soils of Cukurova Region [PhD Thesis]. Adana: Cukurova University, Institute of Science; 2005. 97 p.
 
Laiq ur Rahman AK, Shishir KS, Anand S, Anju Y, Khanuja SPS. Qualitative analysis of essential oil of Rosmarinus officinalis L. cultivated in Uttaranchal Hills India. J Species Arom Crops. 2007;16(1):55-7.
 
Macedo IT, Bevilaqua CM, de Oliveira LM, Camurca-Vasconcelos AL, Vieira Lda S, Oliveira FR, Queiroz-Junior EM, Tome Ada R, Nascimento NR. Anthelmintic effect of Eucalyptus staigeriana essential oil against goat gastrointestinal nematodes. Vet Parasitol. 2010 Oct 11;173(1-2):93-8. https://doi.org/10.1016/j.vetpar.2010.06.004
 
McEvoy JDG. Contamination of animal feedstuffs as a cause of residues in food: A review of regulatory aspects, incidence and control. Anal Chim Acta. 2002;473(1-2):3-26. https://doi.org/10.1016/S0003-2670(02)00751-1
 
Meyer C, Joachim A, Daugschies A. Occurrence of isospora suis in larger piglet production units and on specialized piglet rearing farms. Vet Parasitol. 1999 May;82(4):277-84. https://doi.org/10.1016/S0304-4017(99)00027-8
 
Mizrahi I, Juarez MA, Bandoni AL. The essential oils of Rosmarinus officinalis growing in Argentina. J Essent Oil Res. 1991 Jan-Feb;3(1):11-5. https://doi.org/10.1080/10412905.1991.9697900
 
Mkaddem M, Boussaid M, Ben Fadhel N. Variability of volatiles in Tunisian Mentha pulegium L. (Lamiaceae). J Essent Oil Res. 2007 May-Jun;19(3):211-4. https://doi.org/10.1080/10412905.2007.9699263
 
Negi PS. Plant extracts for the control of bacterial growth: Efficacy, stability and safety issues for food application. Int J Food Microbiol. 2012 May 1;156(1):7-17. https://doi.org/10.1016/j.ijfoodmicro.2012.03.006
 
Odden A, Vatn S, Ruiz A, Robertson LJ, Enemark HL, Nes SK, Tommerberg V, Stuen S. Excretion of Eimeria spp. oocysts in young lambs following iron supplementation. Acta Vet Scand. 2018 Aug 29;60(1):49-59. https://doi.org/10.1186/s13028-018-0404-6
 
Perry BD, Randolph RF, McDermott JJ, Sones KR, Thornton PK. Investing in animal health research to alleviate poverty. Nairobi, Kenya: International Livstock Research Institute (ILRI); 2002. 148 p.
 
Pessoa LM, Morais SM, Bevilaqua CM, Luciano JHS. Anthelmintic activity essential oil of Ocimun gratissimum Linn.and eugenol against Haemonchus contortus. Vet Parasitol. 2002 Oct 16;109(1-2):59-63. https://doi.org/10.1016/S0304-4017(02)00253-4
 
Pintore G, Usai M, Bradesi P, Juliano C, Batto G, Tomi F, Chessa M, Cerri R, Casanova J. Chemical composition and antimicrobial activity of Rosmarinus officinalis L. oils from Sardinia and Corsica. Flavour Fragr J. 2002;17(1):15-9. https://doi.org/10.1002/ffj.1022
 
Pout DD, Ostler DC, Joyner LP, Norton CC. The coccidial population in clinically normal sheep. Vet Rec. 1966 Mar 26;78(13):455-60. https://doi.org/10.1136/vr.78.13.455
 
Riahi L, Elferchichi M, Ghazghazi H, Jebali J, Ziadi S, Aouadhi C, Chograni H, Zaouali Y, Zoghlami N, Mliki A. Phytochemistry, antioxidant and antimicrobial activities of the essential oils of Mentha rotundifolia L. in Tunisia. Ind Crops Prod. 2013 Aug;49:883-;9. https://doi.org/10.1016/j.indcrop.2013.06.032
 
Roepstorff A, Nansen P. Epidemiologie, diagnosis and control of helmith parasites of swine. Rome: FAO; 1998. p. 31-41.
 
Saini R, Davis S, Dudley-Cash W. Oregano essential oil reduces the expression of coccidiosis in broilers. In: Proceeding of the 52nd Conference on Western Poultry Diseases, Sacramento, CA; 2003a. p. 97-8.
 
Saini R, Davis S, Dudley-Cash W. Oregano essential oil reduces necrotic enteritis in broilers. In: Proceeding of the 52nd Conference on Western Poultry Diseases, Sacramento, CA; 2003b. p. 95-7.
 
Saratsis A, Joachim A, Alexandros S, Sotiraki S. Lamb coccidiosis dynamics in different dairy production systems. Vet Parasitol. 2011 Sep 27;181(2-4):131-8. https://doi.org/10.1016/j.vetpar.2011.04.027
 
Shokri A, Saeedi M, Fakhar M, Morteza-Semnani K, Keyghobadi M, Teshnizi SH, Kelidari HR, Sadjadi S. Antileishmanial activity of Lavandula angustifolia and Rosmarinus officinalis essential oils and Nano-emulsions on Leishmania major (MRHO/IR/75/ER). Iran J Parasitol. 2017 Oct-Dec;12(4):622-31.
 
Statsoft. STATISTICA. Windows (Computer program electronic manual). Tulsa, OK: StatSoft Inc.; 1998.
 
Stephan B, Rommel M, Daugschies A, Haberkorn A. Studies of resistance to anticoccidials in Eimeria fied isolates and Eimeria strains. Vet Parasitol. 1997 Apr;69(1-2):19-29. https://doi.org/10.1016/S0304-4017(96)01096-5
 
Szumny A, Figiel A, Gutierrez-Ortiz A, Carbonell-Barrachina AA. Composition of rosemary essential oil (Rosmarinus officinalis) as affected by drying method. J Food Eng. 2010 Mar;97(2):253-60. https://doi.org/10.1016/j.jfoodeng.2009.10.019
 
Taylor MA, Catchpole J. Coccidiosis of domestic ruminants. Appl Parasitol. 1994 Jun; 35(2):73-86.
 
Taylor MA, Coop RL, Wall RL. The epidemiology of parasitic diseases. In: Taylor MA, Wall RL, Coop RL, editors.Veterinary parasitology. 3rd ed. Oxford: Blackwell; 2007. p. 764-9.
 
Taylor MA, Coop RL, Wall RL. Veterinary parasitology. 4th ed. Chichester, West Sussex, UK: Willey-Blackwell; 2016. 1006 p.
 
Tena MT, Valcarcel M, Hidalgo P, Lubera J. Supercritical fluid extraction of natural antioxidants from rosemary: comparison with liquid solvent sonication. Anal Chem. 1997 Feb 1;69(3):521-6. https://doi.org/10.1021/ac960506t
 
Uysal F, Tugrul A. Effect of different plant density on agronomic charachteristics of Rosmarinus officinalis L. In: 8th Field Crops Department Congress, 19–22 October, Hatay, Turkey; 2009.
 
Wada M, Kido H, Ohyama K, Kishikawa N, Ohba Y, Kuroda N, Naka S, Hima K. Evaluation of quenching effects of non-water-soluble and water-soluble rosemary extracts against active oxygen species by chemiluminescent assay. Food Chem. 2004 Sep;87(2):261-7. https://doi.org/10.1016/j.foodchem.2003.11.017
 
Waller PJ. From discovery to development: Current industry perspectives for the development of novel method of helminth control in livestock. Vet. Parasitol. 2006 Jun;139(1–3):1–14. https://doi.org/10.1016/j.vetpar.2006.02.036
 
Waller PJ, Thamsborg SM. Nematode control in green ruminant production systems. Trends Parasitol. 2004 Oct;20(10):493-7. https://doi.org/10.1016/j.pt.2004.07.012
 
Williams RB. Laboratory tests of phenolic disinfectants as oocysticides against the chicken coccidium Eimeria tenella. Vet Rec. 1997 Oct 25;141(17):447-8. https://doi.org/10.1136/vr.141.17.447
 
Wolstenholme AJ, Fairweather I, Prichard R, Von Samson-Himmelstjerna G, Sangster NC. Drug resistance in veterinary helminths. Trends Parasitol. 2004 Oct;20(10):469-76. https://doi.org/10.1016/j.pt.2004.07.010
 
Zhu L, Dai, JL, Yang L, Qiu J. In vitro ovicidal and larvicidal activity of the essential oil of Artemisia lancea against Haemonchus contortus (Strongylida). Vet Parasitol. 2013 Jul 1;195(1-2):112-7. https://doi.org/10.1016/j.vetpar.2012.12.050
 
download PDF

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti