Retrieval action of zinc and folic acid for the restoration of normal reproductive function in bisphenol-A exposed male albino mice

Mustari A, Alam M, Miah MA, Nooruzzaman M, Sujan KM, Chowdhury EH (2022): Retrieval action of zinc and folic acid for the restoration of normal reproductive function in bisphenol-A exposed male albino mice. Vet Med-Czech 67, 479–486.

download PDF

Bisphenol-A (BPA) has become a great concern due to its toxic effects. The present study investigated the retrieval action of zinc (Zn) and folic acid (FA) supplementation against BPA-induced reproductive toxicities in male albino mice. A total of seventy-five 25–28 day-old mice were divided into five equal groups (group A–E, 15 mice in each group). The mice were given normal rations (control, group A) or administered with daily doses of BPA at 50 mg/kg body weight (b.w.) (group B–E). The mice from groups C, D and E were supplemented with Zn (10 mg/kg b.w.), FA (3 mg/kg b.w.) and both in the feed, respectively, daily for 12 weeks. Blood samples were collected, and the sera were separated for biochemical and hormonal analyses. The standard method was followed to test the sperm motility and sperm count. The testis samples were processed for a routine histopathological study using haematoxylin and eosin staining. The sperm counts, motility, and serum testosterone significantly declined in the BPA-exposed animals, but dramatically increased after the Zn and FA supplementation. There was significant degeneration of the seminiferous tubules in the testes of the BPA-exposed mice, which was recovered moderately by the Zn and FA supplementation. The study shows the retrieval action of zinc and folic acid in the restoration of normal reproductive function in bisphenol-A exposed male mice.

Abbasi AA, Prasad AS, Ortega J, Congco E, Oberleas D. Gonadal function abnormalities in sickle cell anemia. Studies in adult male patients. Ann Intern Med. 1976 Nov;85(5):601-5.
Aitken RJ, Smith TB, Jobling MS, Baker MA, De Iuliis GN. Oxidative stress and male reproductive health. Asian J Androl. 2014 Jan-Feb;16(1):31-8.
Aydogan M, Korkmaz A, Barlas N, Kolankaya D. Pro-oxidant effect of vitamin C coadministration with bisphenol A, nonylphenol, and octylphenol on the reproductive tract of male rats. Drug Chem Toxicol. 2010 Apr;33(2):193-203.
Azizollahi G, Azizollahi S, Babaei H, Kianinejad M, Baneshi MR, Nematollahi-mahani SN. Effects of supplement therapy on sperm parameters, protamine content and acrosomal integrity of varicocelectomized subjects. J Assist Reprod Genet. 2013 Apr;30(4):593-9.
Bjornsdotter MK, de Boer J, Ballesteros-Gomez A. Bisphenol A and replacements in thermal paper: A review. Chemosphere. 2017 Sep;182:691-706.
Boxmeer JC, Smit M, Utomo E, Romijn JC, Eijkemans MJ, Lindemans J, Laven JS, Macklon NS, Steegers EA, Steegers-Theunissen RP. Low folate in seminal plasma is associated with increased sperm DNA damage. Fertil Steril. 2009 Aug;92(2):548-56.
Chitra KC, Latchoumycandane C, Mathur PP. Induction of oxidative stress by bisphenol A in the epididymal sperm of rats. Toxicology. 2003 Mar 14;185(1-2):119-27.
Del Val GM, Robledano PM. In vivo serial sampling of epididymal sperm in mice. Lab Anim. 2013 Jul;47(3):168-74.
Forges T, Monnier-Barbarino P, Alberto JM, Gueant-Rodriguez RM, Daval JL, Gueant JL. Impact of folate and homocysteine metabolism on human reproductive health. Hum Reprod Update. 2007 May-Jun;13(3):225-38.
Harcourt AH, Harvey PH, Larson SG, Short RV. Testis weight, body weight and breeding system in primates. Nature. 1981 Sep 3;293(5827):55-7.
Ibrahim W, Tousson E, Ali EM, Mansour MA. Folic acid alleviates oxidative stress and hyperhomocysteinemia involved in testicular dysfunction of hypothyroid rats. Gen Comp Endocrinol. 2011 Nov 1;174(2):143-9.
Kamel AH, Foaud MA, Moussa HM. The adverse effects of bisphenol A on male albino rats. J Basic Appl Zool. 2018 Jan;79(1):1-9.
Khalil N, Ebert JR, Wang L, Belcher S, Lee M, Czerwinski SA, Kannan K. Bisphenol A and cardiometabolic risk factors in obese children. Sci Total Environ. 2014 Feb 1;470-471:726-32.
La Merrill MA, Vandenberg LN, Smith MT, Goodson W, Browne P, Patisaul HB, Guyton KZ, Kortenkamp A, Cogliano VJ, Woodruff TJ, Rieswijk L, Sone H, Korach KS, Gore AC, Zeise L, Zoeller RT. Consensus on the key characteristics of endocrine-disrupting chemicals as a basis for hazard identification. Nat Rev Endocrinol. 2020 Jan;16(1):45-57.
Liu C, Duan W, Li R, Xu S, Zhang L, Chen C, He M, Lu Y, Wu H, Pi H, Luo X, Zhang Y, Zhong M, Yu Z, Zhou Z. Exposure to bisphenol A disrupts meiotic progression during spermatogenesis in adult rats through estrogen-like activity. Cell Death Dis. 2013a Jun 20;4(6):e676.
Liu XL, Chen XY, Wang ZC, Shen T, Zhao H. Effects of exposure to bisphenol A during pregnancy and lactation on the testicular morphology and caspase-3 protein expression of ICR pups. Biomed Rep. 2013b May;1(3):420-4.
Lombo M, Fernandez-Diez C, Gonzalez-Rojo S, Herraez MP. Genetic and epigenetic alterations induced by bisphenol A exposure during different periods of spermatogenesis: From spermatozoa to the progeny. Sci Rep. 2019 Dec 2;9(1):18029.
Luna LG. Manual of histologic staining methods of the armed forces institute of pathology. 3rd ed. New York: McGrow Hill Book Co.; 1968. p. 111-2.
McClain CJ, Gavaler JS, Van Thiel DH. Hypogonadism in the zinc-deficient rat: Localization of the functional abnormalities. J Lab Clin Med. 1984 Dec;104(6):1007-15.
Nakamura D, Yanagiba Y, Duan Z, Ito Y, Okamura A, Asaeda N, Tagawa Y, Li C, Taya K, Zhang SY, Naito H, Ramdhan DH, Kamijima M, Nakajima T. Bisphenol A may cause testosterone reduction by adversely affecting both testis and pituitary systems similar to estradiol. Toxicol Lett. 2010 Apr 15;194(1-2):16-25.
Omu AE, Al-Azemi MK, Al-Maghrebi M, Mathew CT, Omu FE, Kehinde EO, Anim JT, Oriowo MA, Memon A. Molecular basis for the effects of zinc deficiency on spermatogenesis: An experimental study in the Sprague-dawley rat model. Indian J Urol. 2015 Jan-Mar;31(1):57-64.
Prasad AS. Impact of the discovery of human zinc deficiency on health. J Am Coll Nutr. 2009 Jun;28(3):257-65.
Rahman MS, Pang WK, Ryu DY, Park YJ, Pang MG. Multigenerational and transgenerational impact of paternal bisphenol A exposure on male fertility in a mouse model. Hum Reprod. 2020 Aug 1;35(8):1740-52.
Rahman MS, Adegoke EO, Pang MG. Drivers of owning more BPA. J Hazard Mater. 2021a Sep 5;417:126076.
Rahman MS, Pang WK, Ryu DY, Park YJ, Ryu BY, Pang MG. Multigenerational impacts of gestational bisphenol A exposure on the sperm function and fertility of male mice. J Hazard Mater. 2021b Aug 15;416:125791.
Rattan S, Flaws JA. The epigenetic impacts of endocrine disruptors on female reproduction across generations. Biol Reprod. 2019 Sep 1;101(3):635-44.
Ruttkay-Nedecky B, Nejdl L, Gumulec J, Zitka O, Masarik M, Eckschlager T, Stiborova M, Adam V, Kizek R. The role of metallothionein in oxidative stress. Int J Mol Sci. 2013 Mar 15;14(3):6044-66.
Saalu LC, Kpela T, Benebo AS, Oyewopo AO, Anifowope EO, Oguntola JA. The dose-dependent testiculoprotective and testiculotoxic potentials of Telfairia occidentalis Hook F. leaves extract in rat. Int J Appl Res Nat Prod. 2010 Sep;3(3):27-38.
Shalaby MA, El Zorba HY, Ziada RM. Reproductive toxicity of methomyl insecticide in male rats and protective effect of folic acid. Food Chem Toxicol. 2010 Nov;48(11):3221-6.
Skinner MK, Manikkam M, Tracey R, Guerrero-Bosagna C, Haque M, Nilsson EE. Ancestral dichlorodiphenyltrichloroethane (DDT) exposure promotes epigenetic transgenerational inheritance of obesity. BMC Med. 2013 Oct 23;11:228.
Sofikitis N, Giotitsas N, Tsounapi P, Baltogiannis D, Giannakis D, Pardalidis N. Hormonal regulation of spermatogenesis and spermiogenesis. J Steroid Biochem Mol Biol. 2008 Apr;109(3-5):323-30.
Takahashi O, Oishi S. Testicular toxicity of dietary 2,2-bis(4-hydroxyphenyl) propane (bisphenol A) in F344 rats. Arch Toxicol. 2001 Mar;75(1):42-51.
Tian J, Ding Y, She R, Ma L, Du F, Xia K, Chen L. Histologic study of testis injury after bisphenol A exposure in mice. Toxicol Ind Health. 2017 Jan;33(1):36-45.
Tuncer I, Sunar F, Toy H, Baltaci AK, Mogulkoc R. Histological effects of zinc and melatonin on rat testes. Bratisl Lek Listy. 2011;112(8):425-7.
Vandenberg LN, Ehrlich S, Belcher SM, Ben-Jonathan N, Dolinoy DC, Hugo ER, Hunt PA, Newbold RR, Rubin BS, Saili KS. Low dose effects of bisphenol A: An integrated review of in vitro, laboratory animal, and epidemiology studies. Endocr Disruptors. 2013a Sep 30;1(1):26490.
Vandenberg LN, Schaeberle CM, Rubin BS, Sonnenschein C, Soto AM. The male mammary gland: A target for the xenoestrogen bisphenol A. Reprod Toxicol. 2013b Jun;37:15-23.
Wong WY, Merkus HM, Thomas CM, Menkveld R, Zielhuis GA, Steegers-Theunissen RP. Effects of folic acid and zinc sulfate on male factor subfertility: A double-blind, randomized, placebo-controlled trial. Fertil Steril. 2002 Mar;77(3):491-8.
Zago MP, Oteiza PI. The antioxidant properties of zinc: Interactions with iron and antioxidants. Free Radic Biol Med. 2001 Jul 15;31(2):266-74.
Zribi N, Chakroun NF, Elleuch H, Abdallah FB, Ben Hamida AS, Gargouri J, Fakhfakh F, Keskes LA. Sperm DNA fragmentation and oxidation are independent of malondialdheyde. Reprod Biol Endocrinol. 2011 Apr 14;9: [8].
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti