Effects of Secnidazole-diminazene aceturate combination therapy on parasitaemia and serum biochemical profile after late treatment in Trypanosoma brucei brucei infected dogs
Background: Relapse parasitaemia is a major setback in the chemotherapy of late stage Trypanosoma brucei brucei infection. Aberrant serum biochemical profile resulting from T. b. brucei infection in dogs have been attributed to multiple organ injuries resulting from invasive nature of the organism. Therapy with diminazene aceturate alone has not been satisfactory.
Objective: This study evaluated the effects of secnidazole-diminazine aceturate (SEC-DA) combination therapy on parasitaemia and serum biochemical profile after late treatment of T. b. brucei infection in dogs. Methods: Eighteen dogs were randomly assigned to 6 groups (n = 3). Group A: uninfected nor treated; group B: infected without treatment; group C: infected and treated with DA (3.5 mg/kg) (DA-monotherapy) intramuscularly (im) once; group D: infected and treated with SEC (100 mg/kg) and DA (3.5 mg/kg); group E: infected and treated with SEC (200 mg/kg) and DA (3.5 mg/kg) and group F: infected and treated with SEC (400 mg/kg) and DA (3.5 mg/kg). secnidazole was administered orally for 5 days while DA was given im once in groups D – F. Dogs were infected with 5 x 105 trypanosomes intraperitoneally and treatment started day 14 post-infection. Parsitaemia was monitored daily while serum biochemical parameters were monitored on days 14, 21 and 28 post-infections. Results: Total aparasitaemia was achieved in SEC-DA treated dogs 72 h post-treatment and in 86 h in DA-monotherapy dogs. Relapse parasitaemia occurred in DA-monotherapy dogs 17days post-treatment. The SEC-DA combination therapy caused significant (P < 0.05) decline in the hitherto elevated urea and creatinine concentrations, and ALP, ALT, AST activities. Also there was significant (P < 0.05) increase in the previously decreased serum albumin in the SEC-DA treated dogs. Conclusion; Secnidazole-diminazene aceturate combination therapy prevented relapse parasitaemia and ameliorated aberrant serum biochemical profiles of T. b. brucei infected dogs after late treatment.
Relapse parasitaemia; Chemotherapy; Protozoan disease, Tissue invasive; Trypanosomosis