The effect of a bone marrow-derived immunostimulatory preparation on the immunity of broiler chickens vaccinated against salmonellosis N., Kopeikin Y., Litvinova Z. (2019): The effect of a bone marrow-derived immunostimulatory preparation on the immunity of broiler chickens vaccinated against salmonellosis. Veterinarni Medicina, 64: 317-322.
download PDF

The use of bone marrow-derived immunostimulants is a promising direction in poultry production. The objective of this research was to study the effect of introducing a bone marrow cell protein formulation on the immunity of chickens vaccinated against salmonellosis. According to the principle of analogues, a control and two experimental groups of chickens were formed with 20 heads each (in total 60 individuals). To Group 1 birds, a protein preparation from bovine bone marrow cells was administered with feed by irrigation with 10% suspension in physiological saline at a rate of 0.2 ml per head once per day from the first day of life for three days. In Group 2, the drug was administered once, on day 1, at a rate of 0.2 ml per head. Control chickens were injected with saline in the same volumes. All chickens were vaccinated against salmonellosis. Blood for analysis of cellular, biochemical and humoral indicators was taken on days 7 and 14 of bird life. The use of the bone marrow cell-derived protein preparation resulted in higher values in the blood of chickens of Groups 1 and 2, respectively, by day 14 of age in comparison with controls as follows: erythrocytes (15.51% and 22.28%) and leukocytes (3.93% and 3.70%), T- and B- lymphocytes (67.5% and 69.16%; 23.24% and 23.75%), neutrophil phagocytic activity (10.14% and 25.36%) and phagocytic index (17.25% and 18.74%), bactericidal (13.32% and 20.25%) and lysozyme activity (23.88% and 24.41%), total protein (13.23% and 14.21%), immunoglobulins (19.59% and 20.76%), specific antibody titre (47.50% and 51.25%). Our study confirms the suitability of using bone marrow-derived protein preparations in poultry production. In practical terms, our study has particular importance for the development and implementation of preparations based on proteins of bone marrow cells.

Amadori Massimo, Zanotti Cinzia (2016): Immunoprophylaxis in intensive farming systems: the way forward. Veterinary Immunology and Immunopathology, 181, 2-9
Amiranashvili Lia L., Gagelidze Nino A., Makaradze Levan A., Varsimashvili Khatuna I., Tolordava Lana L., Tinikashvili Lela M., Amashukeli Nanuli V., Sachaneli-Qadagishvili Tamar Z., Kirtadze Engur G. (2017): The effect of homoprobiotic preparation “ Probiogeo ” supplemented with drinking water and feed on survivability and growth performance of broiler-chickens. Annals of Agrarian Science, 15, 476-479
Castellini Cesare, Boggia Antonio, Cortina Carla, Dal Bosco Alessandro, Paolotti Luisa, Novelli Emanuele, Mugnai Cecilia (2012): A multicriteria approach for measuring the sustainability of different poultry production systems. Journal of Cleaner Production, 37, 192-201
Chuammitri Phongsakorn, Redmond Sarah B., Kimura Kayoko, Andreasen Claire B., Lamont Susan J., Palić Dušan (2011): Heterophil functional responses to dietary immunomodulators vary in genetically distinct chicken lines. Veterinary Immunology and Immunopathology, 142, 219-227
Chereshnev V. A., Gein S. V., Mazunina L. S., Gavrilova T. V., Chereshneva M. V. (2011): Myelopeptides regulate the microbicidic and secretory activities of innate immunity effectors. Doklady Biochemistry and Biophysics, 436, 53-55
Dorofeychuk VG (1968): Determination of lysozyme activity by nephelometric method (in Russian). Laboratornoe Delo 1, 28–30.
Dzik Sara, Miciński Bartosz, Aitzhanova Indira, Miciński Jan, Pogorzelska Janina, Beisenov Aripzhan, Kowalski Ireneusz M. (2017): Properties of bovine colostrum and the possibilities of use. Polish Annals of Medicine, 24, 295-299
Farag Mayada R., Alagawany Mahmoud (2018): Physiological alterations of poultry to the high environmental temperature. Journal of Thermal Biology, 76, 101-106
Fontana Ilaria, Tullo Emanuela, Butterworth Andy, Guarino Marcella (2015): An innovative approach to predict the growth in intensive poultry farming. Computers and Electronics in Agriculture, 119, 178-183
Gein SV, Mazunina LS (2010): Effect of myelopeptides on the functions of inborn immunity effectors. Basic and applied research in biology and ecology. Perm: PGU Publishing House 8, 104–106. Available at
Gholami Majid, Seidavi Alireza, O'Shea Cormac J., Akter Yeasmin, Dadashbeiki Mohammad, Bahar Bojlul (2017): Feeding regimen of breeder broiler hen influences growth performance of the broiler chickens. Livestock Science, 203, 132-135
Glinskiy Vladimir, Serga Lyudmila, Alekseev Michael, Samotoy Natalia, Simonova Ekaterina (2018): The Development of the Food Industry as a Condition for Improving Russia’s National Security. Procedia Manufacturing, 21, 838-845
Grishko VA, Malina VV, Balatsky YA (2017): Influence of immunostimulants based on thymus and bone marrow on the endocrine profile of prenursery pigs (in Russian). Bulletin of FGOU VPO Bryansk State Agricultural Academy 1, 7–13. Available at
Huber N, Fusani L, Ferretti A, Mahr K, Canoine V (2017): Measuring short-term stress in birds: Comparing different endpoints of the endocrine-immune interface. Physiology & Behavior 182, 46–53.
King T, Osmond-McLeod MJ, Duffy LL (2018): Nanotechnology in the food sector and potential applications for the poultry industry. Trends in Food Science & Technology 72, 62–73.
Korver D.R. (2012): Implications of changing immune function through nutrition in poultry. Animal Feed Science and Technology, 173, 54-64
Kurmanaeva VV (2013): Changes in the immune status of broiler chickens under the influence of biopreparations (in Russian). Bulletin of the Ulyanovsk State Agricultural Academy 2, 74–77. Available at
Mandro NM, Fedorenko TV (2016): Recommendations for the use of a protein preparation from bone marrow cells (in Russian). Blagoveshchensk: Far Eastern State Agrarian University, 19. Available at
Novikov DK (1979): Immunodiagnostic Cell-Based Methods (in Russian). Minsk, Belarus. 222 pp. Available atНовиков%20Дмитри.
Petrov Rem V., Mikhailova Augusta A., Fonina Larissa A. (1997): Bone marrow immunoregulatory peptides (myelopeptides): Isolation, structure, and functional activity. Biopolymers, 43, 139-146<139::AID-BIP7>3.0.CO;2-W
Smirnov PN, Goncharova NB, Voronova IM, Chekishev VM, Vasilyev VS, Nikonov PN, Firsov IV, Mager SN, Razumovskaya VV (1989): Evaluating Natural Resistance of Cattle and Sheep (in Russian). Lenin All-Union Academy of Agricultural Sciences, Siberian Research Institute of Experimental Veterinary and Far East, Novosibirsk. 20 pp.
Wang Xiaofei, Li Yulong, Shen Jing, Wang Siyu, Yao Junhu, Yang Xiaojun (2015): Effect of Astragalus polysaccharide and its sulfated derivative on growth performance and immune condition of lipopolysaccharide-treated broilers. International Journal of Biological Macromolecules, 76, 188-194
Yang Xin, Xin Hongliang, Yang Chengbo, Yang Xiaojun (2018): Impact of essential oils and organic acids on the growth performance, digestive functions and immunity of broiler chickens. Animal Nutrition, 4, 388-393
download PDF

© 2019 Czech Academy of Agricultural Sciences