Presence and antimicrobial resistance of coagulase-negative staphylococci isolated from animals ina Veterinary Teaching Hospital inCyprus

https://doi.org/10.17221/166/2019-VETMEDCitation:Sukur H., Esendal O. (2020): Presence and antimicrobial resistance of coagulase-negative staphylococci isolated from animals in a Veterinary Teaching Hospital in Cyprus. Veterinarni Medicina, 65: 191-198.
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Coagulase-negative staphylococci (CoNS) are a group of commensal microorganisms residing on the skin and mucous membranes of both humans and animals. Until recently, they have been regarded as non-pathogenic to livestock and companion animals, but since then, their clinical importance in veterinary medicine has increased with the discovery of their potential pathogenic roles in animals causing skin and soft tissue infections together with spontaneous abortions and mastitis. Scientific data concerning the presence of CoNS in North Cyprus are very limited. Therefore, the purpose of the study reported herein was to investigate the presence and antimicrobial resistance patterns of CoNS species isolated from various animals presented at the Veterinary Teaching Hospital in North Cyprus between July 2018 and 2019. Staphylococci were isolated from 37.0% (87/235) of the samples submitted, within which 60.9% (53/87) and 39.1% (34/87) were identified as coagulase-positive staphylococci (CoPS) and coagulase-negative staphylococci (CoNS), respectively. Among the CoNS, S. chromogenes was the most predominantly isolated species (14/34, 41.2%), followed byS. capitis (5/34, 14.7%) and S. simulans (4/34, 11.8%). Of these 34 CoNS, 24 (70.6%) and 10 (29.4%) were identified as MRCoNS and MSCoNS, respectively. The CoNS isolates showed relatively high levels of resistance towards amoxicillin/clavulanic acid (19/34, 55.9%), tetracycline (14/34, 41.2%) and penicillin (13/34, 38.2%). In conclusion, the presence of CoNS, especially MRCoNS, and the detection of multiple drug resistant (MDR) species with a high prevalence were regarded as being important since they might limit and have negative effects on the therapeutic treatment options of staphylococcal infections in animals, and might have both public and veterinary concerns.

References:
Alekish MO. The association between the somatic cell count and isolated microorganisms during subclinical mastitis in heifers in Jordan. Vet Med-Czech. 2015 Feb;60(2):71-6. https://doi.org/10.17221/7980-VETMED
 
Ardigo P, D’Incau M, Pongolini S. Abortion in cattle due to infection with Staphylococcus lugdunensis. J Vet Diagn Invest. 2014 Nov;26(6):818-20. https://doi.org/10.1177/1040638714550182
 
Badger-Emeka LI. Co-habitation of Staphylococcus lugdunensis with Staphylococcus aureus resistant to methicillin and vancomycin in the nasal snares of laboratory rats. Adv Microbiol. 2017;7(1):47-55. https://doi.org/10.4236/aim.2017.71004
 
Bhat AM, Soodan JS, Singh R, Dhobi IA, Hussain T, Dar MY, Mir M. Incidence of bovine clinical mastitis in Jammu region and antibiogram of isolated pathogens. Vet World. 2017 Aug;10(8):984. https://doi.org/10.14202/vetworld.2017.984-989
 
Bochniarz M, Wawron W. Antibiotic susceptibility of methicillin-resistant and methicillin-susceptible coagulase-negative staphylococci isolated from bovine mastitis. Pol J Vet Sci. 2011;14(3):405-10. https://doi.org/10.2478/v10181-011-0060-5
 
Cain CL, Morris DO, Rankin SC. Clinical characterization of Staphylococcus schleiferi infections and identification of risk factors for acquisition of oxacillin-resistant strains in dogs: 225 cases (2003–2009). J Am Vet Med Assoc. 2011 Dec 15;239(12):1566-73. https://doi.org/10.2460/javma.239.12.1566
 
Cervinkova D, Vlkova H, Borodacova I, Makovcova J, Babak V, Lorencova A, Vrtkova I, Marosevic D, Jaglic Z. Prevalence of mastitis pathogens in milk from clinically healthy cows. Vet Med-Czech. 2013 Nov;58(11):567-75. https://doi.org/10.17221/7138-VETMED
 
Darbaz I, Bastan A, Salar S. Investigation of udder health and milk quality parameters of dairy farms in Northern Cyprus. Part II: Milk quality. Ankara Univ Vet Fak Derg. 2018 Jun 1;65(2):155-61. https://doi.org/10.1501/Vetfak_0000002841
 
Davis MF, Cain CL, Brazil AM, Rankin SC. Two coagulase-negative staphylococci emerging as potential zoonotic pathogens: Wolves in sheep’s clothing? Front Microbiol. 2013 May 15;4:123. https://doi.org/10.3389/fmicb.2013.00123
 
Dupieux C, Trouillet-Assant S, Tasse J, Freydiere AM, Raulin O, Roure-Sobas C, Salord H, Tigaud S, Laurent F. Evaluation of a commercial immunochromatographic assay for rapid routine identification of PBP2a-positive Staphylococcus aureus and coagulase-negative staphylococci. Diagn Microbiol Infect Dis. 2016 Nov 1;86(3):262-4. https://doi.org/10.1016/j.diagmicrobio.2016.08.012
 
El-Jakee JK, Aref NE, Gomaa A, El-Hariri MD, Galal HM, Omar SA, Samir A. Emerging of coagulase negative staphylococci as a cause of mastitis in dairy animals: An environmental hazard. Int J Vet Sci Med. 2013 Dec 1;1(2):74-8. https://doi.org/10.1016/j.ijvsm.2013.05.006
 
Hosseinzadeh S, Saei HD. Staphylococcal species associated with bovine mastitis in the North West of Iran: Emerging of coagulase-negative staphylococci. Int J Vet Sci Med. 2014 Jun 1;2(1):27-34. https://doi.org/10.1016/j.ijvsm.2014.02.001
 
Karakulska J, Fijalkowski K, Nawrotek P, Pobucewicz A, Poszumski F, Czernomysy-Furowicz D. Identification and methicillin resistance of coagulase-negative staphylococci isolated from nasal cavity of healthy horses. J Microbiol. 2012 Jun 1;50(3):444-51. https://doi.org/10.1007/s12275-012-1550-6
 
Kern A, Perreten V. Clinical and molecular features of methicillin-resistant, coagulase-negative staphylococci of pets and horses. J Antimicrob Chemother. 2013 Jun 1;68(6):1256-66. https://doi.org/10.1093/jac/dkt020
 
Loncaric I, Kunzel F, Klang A, Wagner R, Licka T, Grunert T, Fessler AT, Geier-Domling D, Rosengarten R, Muller E, Reissig A. Carriage of meticillin-resistant staphylococci between humans and animals on a small farm. Vet Dermatol. 2016 Jun;27(3):191-e48. https://doi.org/10.1111/vde.12321
 
Mallardo K, Nizza S, Fiorito F, Pagnini U, De Martino L. A comparative evaluation of methicillin-resistant staphylococci isolated from harness racing-horses, breeding mares and riding-horses in Italy. Asian Pac J Trop Biomed. 2013 Mar 1;3(3):169-73. https://doi.org/10.1016/S2221-1691(13)60044-1
 
Metiner K, Bagcigil AF, Ilgaz A. Determination of the diversity and antibiotic resistance profiles of Staphylococcus species from dogs with otitis externa and examination of mecA gene occurrence. Vet Med-Czech. 2015 May;60(5):261-7. https://doi.org/10.17221/8178-VETMED
 
Nobrega DB, Naushad S, Naqvi SA, Condas LA, Saini V, Kastelic JP, Luby C, De Buck J, Barkema HW. Prevalence and genetic basis of antimicrobial resistance in non-aureus staphylococci isolated from Canadian dairy herds. Front Microbiol. 2018 Feb 16;9:256. https://doi.org/10.3389/fmicb.2018.00256
 
Qekwana DN, Sebola D, Oguttu JW. Antimicrobial resistance patterns of Staphylococcus species isolated from cats presented at a veterinary academic hospital in South Africa. BMC Vet Res. 2017 Dec 1;13(1):286. https://doi.org/10.1186/s12917-017-1204-3
 
Rook KA, Brown DC, Rankin SC, Morris DO. Case-control study of Staphylococcus lugdunensis infection isolates from small companion animals. Vet Dermatol. 2012 Dec;23(6):476-e90. https://doi.org/10.1111/j.1365-3164.2012.01087.x
 
Ruzauskas M, Siugzdiniene R, Klimiene I, Virgailis M, Mockeliunas R, Vaskeviciute L, Zienius D. Prevalence of methicillin-resistant Staphylococcus haemolyticus in companion animals: A cross-sectional study. Ann Clin Microbiol Antimicrob. 2014 Dec 1;13(1):56. https://doi.org/10.1186/s12941-014-0056-y
 
Siugzdaite J, Gabinaitiene A. Methicillin-resistant coagulase-negative staphylococci in healthy dogs. Vet Med-Czech. 2017 Sep;62(9):479-87. https://doi.org/10.17221/96/2015-VETMED
 
Srednik ME, Archambault M, Jacques M, Gentilini ER. Detection of a mecC-positive Staphylococcus saprophyticus from bovine mastitis in Argentina. J Glob Antimicrob Resist. 2017 Sep 1;10:261-3. https://doi.org/10.1016/j.jgar.2017.05.016
 
Vakkamaki J, Taponen S, Heikkila AM, Pyorala S. Bacteriological etiology and treatment of mastitis in Finnish dairy herds. Acta Vet Scand. 2017 Dec;59(1):33. https://doi.org/10.1186/s13028-017-0301-4
 
Vanderhaeghen W, Piepers S, Leroy F, Van Coillie E, Haesebrouck F, De Vliegher S. Identification, typing, ecology and epidemiology of coagulase negative staphylococci associated with ruminants. Vet J. 2015 Jan 1;203(1):44-51. https://doi.org/10.1016/j.tvjl.2014.11.001
 
Zigo F, Elecko J, Vasil M, Ondrasovicova S, Farkasova Z, Malova J, Takac L, Zigova M, Bujok J, Pecka-Kielb E, Timkovicova-Lackova P. The occurrence of mastitis and its effect on the milk malondialdehyde concentrations and blood enzymatic antioxidants in dairy cows. Vet Med-Czech. 2019 Oct;64(10):423-32. https://doi.org/10.17221/67/2019-VETMED
 
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