Salmonella enterica subspecies enterica serovar Paratyphi B as a disease-causing agent in reptiles in the Czech Republic
J. Bzdil, O. Holy, J. Toporcakhttps://doi.org/10.17221/174/2016-VETMEDCitation:Bzdil J., Holy O., Toporcak J. (2017): Salmonella enterica subspecies enterica serovar Paratyphi B as a disease-causing agent in reptiles in the Czech Republic. Veterinarni Medicina, 62: 410-415.
The aim of this study was to describe two case studies of reptile disease, in which the primary pathogen was apparently Salmonella enterica subspecies enterica serovar Paratyphi B. Pathological examinations, sampling from different organs and cloacal swabs was performed on a dead savannah monitor (Varanus exanthematicus) and a sick green tree python (Chondropython viridis). This material was subjected to culture examination, including selective enrichment using standard methods. Typing was performed using MALDI-TOF and strains were also serologically typified. The utilisation of d-tartrate was confirmed biochemically and also using PCR. Antibiotic susceptibility was determined by the standard disc diffusion method using Mueller-Hinton agar without blood and antibiotic discs. In both cases the detected Salmonella enterica subspecies enterica serovar Paratyphi B was positive for d-tartrate and exquisitely susceptible to chloramphenicol, tetracycline, ampicillin, amoxycillin/clavulanic acid, gentamicin, ceftazidime, enrofloxacin and piperacillin.Keywords:
paratyphoid; Varanus exanthematicus; Chondropython viridis; d-tartrate; treatmentReferences:
Bzdil J (2003): Determination of susceptibility of bacteria to antimicrobials by disc diffusion method (in Czech). Standard Operating Procedure of SVI Olomouc, 1–4.Bzdil J, Kubickova I, Kvapilova M (2012): Salmonella occurence in clinical material in human and veterinary sphere in period 2006–2010 (in Czech). Veterinarstvi 10, 625–629.Chart H. (2003): The pathogenicity of strains of Salmonella paratyphi B and Salmonella java. Journal of Applied Microbiology, 94, 340-348 https://doi.org/10.1046/j.1365-2672.2003.01863.xThrelfall E. John, Bhutta Zulfiqar, Alam Didar, Nizami Qamarauddin, Wain John, Siorvanes Lisa, de Pinna Elizabeth, Cheasty Thomas, Chart Henrik (2007): Serodiagnosis of Salmonella enterica serovar Typhi and S. enterica serovars Paratyphi A, B and C human infections. Journal of Medical Microbiology, 56, 1161-1166 https://doi.org/10.1099/jmm.0.47197-0Evans S. J., Davies R. H., Binns S. H., Liebana E., Jones T. W. H., Millar M. F., Threlfall E. J., Ward L. R., Hopkins K. L., Mackay P. H. S., Gayford P. J. R. (2005): Multiple antimicrobial resistant Salmonella enterica serovar Paratyphi B variant Java in cattle: a case report. Veterinary Record, 156, 343-346 https://doi.org/10.1136/vr.156.11.343Hernandez E, Rodriguez JL, Herrera-Leon S, Garcia I, de Castro V, Muniozguren N (2012): Salmonella Paratyphi B var Java infections associated with exposure to turtles in Bizkaia, Spain, September 2010 to October 2011. Euro Surveillance 17, 6–10.Kauffmann F (1941): A typhoid variant and a new serological variation in the Salmonella group. Journal of Bacteriology 41, 127–140.MARTINEZ-URTAZA JAIME, ECHEITA AURORA, LIEBANA ERNESTO (2006): Phenotypic and Genotypic Characterization of Salmonella enterica Serotype Paratyphi B Isolates from Environmental and Human Sources in Galicia, Spain. Journal of Food Protection, 69, 1280-1285 https://doi.org/10.4315/0362-028X-69.6.1280Miko A., Guerra B., Schroeter A., Dorn C., Helmuth R. (2002): Molecular Characterization of Multiresistant d-Tartrate-Positive Salmonella enterica Serovar Paratyphi B Isolates. Journal of Clinical Microbiology, 40, 3184-3191 https://doi.org/10.1128/JCM.40.9.3184-3191.2002Singh Bhoj Raj, Jyoti Jatinder, Chandra Mudit, Babu N., Sharma G. (2009): Drug resistance patterns of Salmonella isolates of equine origin from India. The Journal of Infection in Developing Countries, 3, - https://doi.org/10.3855/jidc.61Stenglein M. D., Sanders C., Kistler A. L., Ruby J. G., Franco J. Y., Reavill D. R., Dunker F., DeRisi J. L. (): Identification, Characterization, and In Vitro Culture of Highly Divergent Arenaviruses from Boa Constrictors and Annulated Tree Boas: Candidate Etiological Agents for Snake Inclusion Body Disease. mBio, 3, e00180-12-e00180-12 https://doi.org/10.1128/mBio.00180-12Toboldt A., Tietze E., Helmuth R., Fruth A., Junker E., Malorny B. (): Human Infections Attributable to the D-Tartrate-Fermenting Variant of Salmonella enterica Serovar Paratyphi B in Germany Originate in Reptiles and, on Rare Occasions, Poultry. Applied and Environmental Microbiology, 78, 7347-7357 https://doi.org/10.1128/AEM.01732-12