Effects of humic acid diet on the serum biochemistry and oxidative status markers in pheasants

https://doi.org/10.17221/174/2019-VETMEDCitation:Kovacik A., Gasparovic M., Tvrda E., Tokarova K., Kovacikova E., Rolinec M., Rumanova L., Capcarova M., Galik B. (2020): Effects of humic acid diet on the serum biochemistry and oxidative status markers in pheasants. Veterinarni Medicina, 65: 258-268.
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This study investigated the effect of different concentrations of humic acids (HAs) on the selected serum biochemistry parameters and oxidative status markers in common pheasants (Phasianus colchicus). The control birds were fed a diet with no HA additives, while the birds in the experimental groups were fed diets containing HAs at the level of 0.5% (EG1), 0.75% (EG2) and 1.0% (EG3) from 1 to 90 days of age.  The blood sodium concentration decreased (P < 0.01) in the group fed by 1.00% HAs compared to the control birds. The concentration of potassium in the EG2 group increased (P < 0.05) in comparison to the control group. The EG1 group showed a higher (P < 0.01) serum glucose than the EG2 group. Significant differences (P < 0.05; P < 0.01) were also found between the experimental groups (EG1 vs EG2, and EG2 vs EG3) in the cholesterol concentrations. The birds in the experimental groups showed lower ROS (reactive oxygen species) and MDA (malondialdehyde) production. An opposite effect was observed in the TAC (total antioxidant capacity), where its values significantly increased in the experimental groups. The diet supplementation affected the enzymatic antioxidant system of the fattened pheasants, and so the HAs exhibited an antioxidant potential in these birds.

References:
Afonso V, Champy R, Mitrovic D, Collin P, Lomri A. Reactive oxygen species and superoxide dismutases: Role in joint diseases. Joint Bone Spine. 2007 Jul;74(4):324-9. https://doi.org/10.1016/j.jbspin.2007.02.002
 
Agazzi A, Cigalino G, Mancin G, Savoini G, Dell’Orto V. Effects of dietary humates on growth and an aspect of cell-mediated immune response in newborn kids. Small Rum Res. 2007 Oct 1;72(2-3):242-5. https://doi.org/10.1016/j.smallrumres.2006.10.020
 
Akbas A, Silan C, Gulpinar MT, Sancak EB, Ozkanli SS, Cakir DU. Renoprotective effect of humic acid on renal ischemia-reperfusion injury: An experimental study in rats. Inflammation. 2015 Dec;38(6):2042-8. https://doi.org/10.1007/s10753-015-0185-2
 
Andrejcakova Z, Sopkova D, Vlckova R, Kulichova L, Gancarcikova S, Almasiova V, Holovska K, Petrilla V, Kresakova L. Synbiotics suppress the release of lactate dehydrogenase, promote non-specific immunity and integrity of jejunum mucosa in piglets. Anim Sci J. 2016 Sep;87(9):1157-66. https://doi.org/10.1111/asj.12558
 
Arpasova H, Kacaniova M, Pistova V, Galik B, Fik M, Hleba L. Effect of probiotics and humic acid on egg production and quality parameters of laying hens eggs. Sci Pap Anim Sci Biotechnol./Lucr Stiint Zooteh Bioteh. 2016 Jul 1;49(2):1-9.
 
Avci M, Denek N, Kaplan O. Effects of humic acid at different levels on growth performance, carcass yields and some biochemical parameters of quails. J Anim Vet Adv. 2007 Jan 1;6(1):1-4.
 
Ayuso M, Moreno JL, Hernandez T, Garcia C. Characterisation and evaluation of humic acids extracted from urban waste as liquid fertilisers. J Sci Food Agric. 1997 Dec;75(4):481-8. https://doi.org/10.1002/(SICI)1097-0010(199712)75:4<481::AID-JSFA901>3.0.CO;2-K
 
Balos MZ, Jaksic S, Knezevic S, Kapetanov M. Electrolytes–sodium, potassium and chlorides in poultry nutrition. Arh Vet Med. 2016;9(1):31-42.
 
Beers RF Jr, Sizer IW. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem. 1952 Mar;195(1):133-40.
 
Cusack PM. Effects of a dietary complex of humic and fulvic acids (FeedMAX 15) on the health and production of feedlot cattle destined for the Australian domestic market. Aust Vet J. 2008 Jan-Feb;86(1-2):46-9. https://doi.org/10.1111/j.1751-0813.2007.00242.x
 
Denbow DM. Gastrointestinal anatomy and physiology. In: Scanes CG, editor. Sturkie’s avian physiology. 6th ed. London, UK: Academic Press; 2015. p. 337-66.
 
Dzikamunhenga RS, Griffith RW, Hostetter S, Fisher P, Larson W. Hematology and serum biochemistry reference intervals for six-week-old, farm-reared Chinese ring-necked pheasants (Phasianus colchicus ) from Minnesota. Avian Dis. 2017 Jun;61(2):211-3. https://doi.org/10.1637/11588-011017-Reg.1
 
Doneley B. Avian medicine and surgery in practice. Boca Raton: CRC Press; 2016. 467 p.
 
Efimova IV, Khil’ko SL, Smirnova OV. Antioxidant activity of humic acids in radical-chain oxidation processes. Russ J Appl Chem. 2012 Sep 1;85(9):1351-4. https://doi.org/10.1134/S1070427212090091
 
EMEA – The European Agency for the Evaluation of Medicinal Products. Humic acids and their sodium salts – Summary report [Internet]. Committee for Veterinary Medicinal Products. 1999 [cited 2019 Nov 28]. Available from www.ema.europa.eu/documents/mrl-report/humic-acids-their-sodium-salts-summary-report-committee-veterinary-medicinal-products_en.pdf.
 
Fingerova H, Novotny J, Barborik J, Brezinova J, Svobodova M, Krskova M, Oborna I. Antioxidant capacity of seminal plasma measured by TAS Randox. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2007 Jun;151(1):37-40. https://doi.org/10.5507/bp.2007.006
 
Forbes NA, Guzman DSM. Avian medicine and surgery: Self-assessment color review. Boca Raton: CRC Press; 2017. 364 p.
 
Gasparovic M, Hrncar C, Galik B. The effect of feed additives in pheasants fattening: A review. J Cent Eur Agric. 2017 Dec 1;18(4):749-62. https://doi.org/10.5513/JCEA01/18.4.1953
 
Genc-Fuhrman H, Mikkelsen PS, Ledin A. Simultaneous removal of As, Cd, Cr, Cu, Ni and Zn from stormwater using high-efficiency industrial sorbents: Effect of pH, contact time and humic acid. Sci Total Environ. 2016 Oct 1;566-567:76-85. https://doi.org/10.1016/j.scitotenv.2016.04.210
 
Harris DJ. Clinical tests. In: Tully TN, Dorrestein GM, Jones AK, editors. Handbook of avian medicine. 2nd ed. Oxford, UK: Elsevier; 2009. p. 77-84.
 
Hocking PM, Mitchell MA, Bernard R, Sandercock DA. Interaction of age, strain, sex and food restriction on plasma creatine kinase activity in turkeys. Br Poult Sci. 1998 Jul;39(3):360-4. https://doi.org/10.1080/00071669888908
 
Ipek H, Avci M, Iriadam M, Kaplan O, Denek N. Effects of humic acid on some hematological parameters, total antioxidant capacity and laying performance in Japanese quails. Arch Geflugelkd. 2008 Apr 1;72(2):56-60.
 
Jerabek M, Suchy P, Strakova E, Kudelkova L, Simek V, Jakesova P, Machacek M, Zapletal D. Selected blood biochemical indicators of Cherry Valley ducks undergoing fattening in relation to their diet and sex. Vet Med-Czech. 2018 Sep 18;63(9):420-32. https://doi.org/10.17221/81/2018-VETMED
 
Ji F, McGlone JJ, Kim SW. Effects of dietary humic substances on pig growth performance, carcass characteristics, and ammonia emission. J Anim Sci. 2006 Sep;84(9):2482-90. https://doi.org/10.2527/jas.2005-206
 
Jones MN, Bryan ND. Colloidal properties of humic substances. Adv Colloid Interface Sci. 1998 Aug 1;78(1):1-48. https://doi.org/10.1016/S0001-8686(98)00058-X
 
Karaoglu M, Macit M, Esenbuga N, Durdag H, Turgut L, Bilgin PC. Effect of supplemental humate at different levels on the growth performance, slaughter and carcass traits of broilers. Int J Poult Sci. 2004;3(6):406-10. https://doi.org/10.3923/ijps.2004.406.410
 
Kashou AH, Sharma R, Agarwal A. Assessment of oxidative stress in sperm and semen. Methods Mol Biol. 2013;927:351-61.
 
Kawai T. Clinical aspects of the plasma proteins. Berlin/Heidelberg: Springer Science & Business Media; 1973. 464 p.
 
Kececi T, Col R. Haematological and biochemical values of the blood of pheasants (Phasianus colchicus) of different ages. Turk J Vet Anim Sci. 2011 Jun 30;35(3):149-56.
 
Khan SU. Adsorption of pesticide by humic substances. A review. Environ Lett. 1972;3(1):1-12. https://doi.org/10.1080/00139307209435449
 
Kocabagli N, Alp M, Acar N, Kahraman R. The effects of dietary humate supplementation on broiler growth and carcass yield. Poult Sci. 2002 Feb;81(2):227-30. https://doi.org/10.1093/ps/81.2.227
 
Kovacik A, Arvay J, Tusimova E, Harangozo L, Tvrda E, Zbynovska K, Cupka P, Andrascikova S, Tomas J, Massanyi P. Seasonal variations in the blood concentration of selected heavy metals in sheep and their effects on the biochemical and hematological parameters. Chemosphere. 2017 Feb;168:365-71. https://doi.org/10.1016/j.chemosphere.2016.10.090
 
Kovacik A, Tvrda E, Miskeje M, Arvay J, Tomka M, Zbynovska K, Andreji J, Hleba L, Kovacikova E, Fik M, Cupka P, Nahacky J, Massanyi P. Trace metals in the freshwater fish Cyprinus carpio: Effect to serum biochemistry and oxidative status markers. Biol Trace Elem Res. 2019 Apr;188(2):494-507. https://doi.org/10.1007/s12011-018-1415-x
 
Kucukersan S, Kucukersan K, Colpan I, Goncuoglu E, Reisli Z, Yesilbag D. The effects of humic acid on egg production and egg traits of laying hen. Vet Med-Czech. 2005 Sep 1;50(9):406-10. https://doi.org/10.17221/5640-VETMED
 
Neumeier D, Jockers-Wretou E. Tissue specific and subcellular distribution of creatine kinase isoenzymes. In: Lang H, editor. Creatine kinase isoenzymes. Berlin/Heidelberg: Springer-Verlag; 1981. p. 85-131.
 
Lala AO, Okwelum N, Bello KO, Famakinde NA, Alamu MO. Comparative study between ISA Brown and Fulani Ecotype chickens supplemented with humic acid. Slovak J Anim Sci. 2016 Jun 30;49(2):68-75.
 
Lloyd S, Gibson JS. Haematology and biochemistry in healthy young pheasants and red-legged partridges and effects of spironucleosis on these parameters. Avian Pathol. 2006 Aug;35(4):335-40. https://doi.org/10.1080/03079450600821794
 
Lovley DR, Coates JD, Blunt-Harris EL, Phillips EJ, Woodward JC. Humic substances as electron acceptors for microbial respiration. Nature. 1996 Aug;382(6590):445-8. https://doi.org/10.1038/382445a0
 
Lumeij JT. The diagnostic value of plasma proteins and non-protein nitrogen substances in birds. Vet Q. 1987 Jul;9(3):262-8. https://doi.org/10.1080/01652176.1987.9694111
 
Lumeij JT, Westerhof I. Blood chemistry for the diagnosis of hepatobiliary disease in birds. A review. Vet Q. 1987 Jul;9(3):255-61. https://doi.org/10.1080/01652176.1987.9694110
 
Lumeij JT. Avian clinical biochemistry. In: Kaneko JJ, Harvey JW, Bruss ML, editors. Clinical biochemistry of domestic animals. San Diego, CA: Academic Press; 1997. p. 857-83.
 
Massanyi P, Stawarz R, Halo M, Formicki G, Lukac N, Cupka P, Schwarcz P, Kovacik A, Tusimova E, Kovacik J. Blood concentration of copper, cadmium, zinc and lead in horses and its relation to hematological and biochemical parameters. J Environ Sci Health A Tox Hazard Subst Environ Eng. 2014;49(8):973-9. https://doi.org/10.1080/10934529.2014.894322
 
Mikkelsen RL. Humic materials for agriculture. Better Crop. 2005;89(3):6-10.
 
Muller CH, Lee TKY, Montano MA. Improved chemiluminescence assay for measuring antioxidant capacity of seminal plasma. In: Carrell DA, Aston KI, editors. Spermatogenesis. Methods and protocols. 1st ed. New York, NY: Springer Science + Business Media; 2013. p. 363-76.
 
Mvila BG, Pilar-Izquierdo MC, Busto MD, Perez-Mateos M, Ortega N. Synthesis and characterization of a stable humic-urease complex: Application to barley seed encapsulation for improving N uptake. J Sci Food Agric. 2016 Jul;96(9):2981-9.  https://doi.org/10.1002/jsfa.7466
 
Nazifi S, Mosleh N, Ranjbar VR, Khordadmehr M. Reference values of serum biochemical parameters in adult male and female ring-necked pheasants (Phasianus colchicus). Comp Clin Path. 2012 Oct 1;21(5):981-4. https://doi.org/10.1007/s00580-011-1212-9
 
Ozkan A, Sen HM, Sehitoglu I, Alacam H, Guven M, Aras AB, Akman T, Silan C, Cosar M, Karaman HI. Neuroprotective effect of humic Acid on focal cerebral ischemia injury: An experimental study in rats. Inflammation. 2015 Feb;38(1):32-9. https://doi.org/10.1007/s10753-014-0005-0
 
Ozturk E, Ocak N, Coskun I, Turhan S, Erener G. Effects of humic substances supplementation provided through drinking water on performance, carcass traits and meat quality of broilers. J Anim Physiol Anim Nutr (Berl). 2010 Feb 1;94(1):78-85. https://doi.org/10.1111/j.1439-0396.2008.00886.x
 
Ozturk E, Ocak N, Turan A, Erener G, Altop A, Cankaya S. Performance, carcass, gastrointestinal tract and meat quality traits, and selected blood parameters of broilers fed diets supplemented with humic substances. J Sci Food Agric. 2012 Jan 15;92(1):59-65. https://doi.org/10.1002/jsfa.4541
 
Pena-Mendez EM, Havel J, Patocka J. Humic substances – compounds of still unknown structure: Applications in agriculture, industry, environment, and biomedicine. J Appl Biomed. 2005 Mar 31;3(1):13-24. https://doi.org/10.32725/jab.2005.002
 
Rath NC, Huff WE, Huff GR. Effects of humic acid on broiler chickens. Poult Sci. 2006 Mar;85(3):410-4. https://doi.org/10.1093/ps/85.3.410
 
Rath S, Fostier AH, Pereira LA, Dioniso AC, de Oliveira Ferreira F, Doretto KM, Maniero Peruchi L, Viera A, de Oliveira Neto OF, Dal Bosco SM, Martinez-Mejia MJ. Sorption behaviors of antimicrobial and antiparasitic veterinary drugs on subtropical soils. Chemosphere. 2019 Jan;214:111-22. https://doi.org/10.1016/j.chemosphere.2018.09.083
 
Russo R, Pucci L, Giorgetti L, Arvay J, Vizzarri F, Longo V, Pozzo L. Polyphenolic characterisation of plant mixture (Lisosan® Reduction) and its hypocholesterolaemic effect in high fat diet-fed mice. Nat Prod Res. 2019 Mar;33(5):651-8. https://doi.org/10.1080/14786419.2017.1402328
 
Samour J. Avian medicine. 3rd ed. St. Louis, MO: Mosby International Ltd; 2016. 608 p.
 
Samudovska A, Demeterova M. Effect of diet supplemented with natural humic compounds and sodium humate on performance and selected metabolic variables in broiler chickens. Acta Vet Brno. 2010 Nov 1;79(3):385-93. https://doi.org/10.2754/avb201079030385
 
Sandercock DA, Hunter RR, Nute GR, Mitchell MA, Hocking PM. Acute heat stress-induced alterations in blood acid-base status and skeletal muscle membrane integrity in broiler chickens at two ages: Implications for meat quality. Poult Sci. 2001 Apr;80(4):418-25. https://doi.org/10.1093/ps/80.4.418
 
Shah MU, Zaneb H, Masood S, Qureshi AS, Ullah HA, Sikandar A, Din SA, Ahmad IJ, Khan MS, Rehman HU, Usman MU. Effect of single or combined supplementation of zinc and probiotics on muscle and bone characteristics and haematobiochemical profile in broilers. Vet Med-Czech. 2020 Mar 28;65(3):134-42. https://doi.org/10.17221/152/2019-VETMED
 
Simos YV, Verginadis II, Toliopoulos IK, Velalopoulou AP, Karagounis IV, Karkabounas SC, Evangelou AM. Effects of catechin and epicatechin on superoxide dismutase and glutathione peroxidase activity, in vivo. Redox Rep. 2012;17(5):181-6. https://doi.org/10.1179/1351000212Y.0000000020
 
Smirnova OV, Efimova IV, Khil’ko SL. Antioxidant and pro-oxidant activity of ascorbic and humic acids in radical-chain oxidation processes. Russ J Appl Chem. 2012 Feb 1;85(2):252-5. https://doi.org/10.1134/S1070427212020164
 
Sladecek T, Jurcik R, Slamecka J, Ondruska L Effect of humic substances on the reproduction parameters of farmed brown hare. Slovak J Anim Sci. 2018 Jun 30;51(2):86-90.
 
Stevenson FJ. Humus chemistry: Genesis, composition, reactions. New York: John Wiley & Sons; 1994. p. 496.
 
Supriyati, Haryati T, Susanti T, Susana IW. Nutritional value of rice bran fermented by Bacillus amyloliquefaciens and humic substances and its utilization as a feed ingredient for broiler chickens. Asian-Australas J Anim Sci. 2015 Feb;28(2):231-8. https://doi.org/10.5713/ajas.14.0039
 
Tang WW, Zeng GM, Gong JL, Liang J, Xu P, Zhang C, Huang BB. Impact of humic/fulvic acid on the removal of heavy metals from aqueous solutions using nanomaterials: A review. Sci Total Environ. 2014 Jan 15;468-469:1014-27. https://doi.org/10.1016/j.scitotenv.2013.09.044
 
Tvrda E, Tusimova E, Kovacik A, Paal D, Greifova H, Abdramanov A, Lukac N. Curcumin has protective and antioxidant properties on bull spermatozoa subjected to induced oxidative stress. Anim Reprod Sci. 2016 Sep 1;172:10-20.  https://doi.org/10.1016/j.anireprosci.2016.06.008
 
Valuev LI, Valueva TA, Valuev IL, Plate NE. Polymer systems for controlled release of biologically active compounds. Usp Biol Khim. 2003;43:307-28.
 
Vizzarri F, Nardoia M, Palazzo M. Effect of dietary Lippia citriodora extract on productive performance and meat quality parameters in hares (Lepus europaeus Pall.). Arch Anim Breed. 2014 Jun 30;57(1):1-7. https://doi.org/10.7482/0003-9438-57-020
 
Wang Q, Chen YJ, Yoo JS, Kim HJ, Cho JH, Kim IH. Effects of supplemental humic substances on growth performance, blood characteristics and meat quality in finishing pigs. Livest Sci. 2008 Sep 1;117(2-3):270-4. https://doi.org/10.1016/j.livsci.2007.12.024
 
Yoruk MA, Gul M, Hayirli A, Macit M. The effects of supplementation of humate and probiotic on egg production and quality parameters during the late laying period in hens. Poult Sci. 2004;83(1):84-8. https://doi.org/10.1093/ps/83.1.84
 
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