Immunohistochemical characterisation of neurons in the mandibular ganglion and nerve fibres supplying the porcine mandibular gland M., Podlasz P., Sienkiewicz W., Franke-Radowiecka A., Dudek A., Pidsudko Z., Chmielewska-Krzesinska M., Kaleczyc J. (2016): Immunohistochemical characterisation of neurons in the mandibular ganglion and nerve fibres supplying the porcine mandibular gland. Veterinarni Medicina, 61: 361-373.
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The present study was designed to investigate the chemical coding of neurons in the mandibular ganglion (MGn) and nerve fibres supplying the porcine mandibular gland (MGl) with the use of immunofluorescence and RT-PCR. The cryostat sections from MGn and MGl were processed for double-labelling immunohistochemistry using antisera against vesicular acetylcholine transporter (VAChT), choline acetyltransferase (ChAT), dopamine β-hydroxylase (DβH), neuronal nitric oxide synthase (nNOS), vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY), galanin (GAL), substance P (SP) and calcitonin gene-related peptide (CGRP). The MGl was found to be richly supplied by VAChT-positive nerve fibres that surrounded intra- and interlobular salivary ducts. A large number of VAChT-immunoreactive (VAChT-IR) nerve terminals were also observed around acini. Many periductal and periacinar nerve fibres stained positive for DβH. Immunoreactivity to GAL, NPY or VIP was observed in an intermediate number of nerve terminals which were associated with both salivary ducts and acini. Double-immunostaining revealed that in MGn nearly all neurons stained positive for VAChT/ChAT (98.45 ± 0.59%, mean ± SEM) and nNOS (99.71 ± 0.18%). An intermediate number of the nerve cell bodies displayed immunoreactivity to NPY or VIP (18.67 ± 0.52% and 8.11 ± 0.36%, respectively). Single GAL-IR and CGRP-positive neurons were also observed. RT-PCR revealed the presence of transcripts of ChAT, VAChT, nNOS, NPY, VIP and GAL. For SP and DβH very weak signals were observed. RT-PCR with primers targeting CGRP did not generate any PCR product.
Alm P, Ekstrom J, Larsson B, Tobin G, Andersson KE (1997): Nitric oxide synthase immunoreactive nerves in rat and ferret salivary glands, and effects of denervation. Histochemistry Journal 29, 669−676.
Arciszewski MB, Zacharko A (2003): Distribution of sympathetic and afferent neurones innervating the submandibular gland in the sheep. Anatomia Histologia Embryologia 32, 316−319.
Arciszewski M. B., Zacharko A., Lalak R. (2004): Co-expression of tyrosine hydroxylase, dopamine beta-hydroxylase and neuropeptide Y in the sympathetic neurons projecting to the submandibular gland in the sheep. Anatomy and Embryology, 208, 161-167
Bloom GD, Carlsoo B (1973): Adrenergic and cholinergic nerves of bovine, guinea pig and hamster salivary glands. Zeitschrift fur Zellforschung und Mikroskopische Anatomie 138, 407−420.
Booth W. D. (1980): A study of some major testicular steroids in the pig in relation to their effect on the development of male characteristics in the prepubertally castrated boar. Reproduction, 59, 155-162
Brain S. D., Williams T. J., Tippins J. R., Morris H. R., MacIntyre I. (1985): Calcitonin gene-related peptide is a potent vasodilator. Nature, 313, 54-56
Chibuzo GA, Cummings JF (1980): Motor and sensory centers for the innervation of mandibular and sublingual salivary glands: a horseradish peroxidase study in the dog. Brain Research 189, 301−313.
Del Fiacco M, Quartu M, Ekstrom J, Melis T, Boi M, Isola M, Loy F, Serra M (2014): Effect of the neuropeptides vasoactive intestinal peptide, peptide histidine methionine and substance P on human major salivary gland secretion. Oral Diseases 21, 216−223.
Geerling Gerd, Garrett John R., Paterson Katherine L., Sieg Peter, Collin J Richard O., Carpenter Guy H., Hakim Samer G., Lauer Isabel, Proctor Gordon B. (2008): Innervation and Secretory Function of Transplanted Human Submandibular Salivary Glands. Transplantation, 85, 135-140
Gibbins I.L. (1991): Vasomotor, pilomotor and secretomotor neurons distinguished by size and neuropeptide content in superior cervical ganglia of mice. Journal of the Autonomic Nervous System, 34, 171-183
Hauser-Kronberger C., Albegger K., Saria A., Hacker G. W. (): Neuropeptides in Human Salivary (Submandibular and Parotid) Glands. Acta Oto-Laryngologica, 112, 343-348
Jia X, Jia Y, Wang H, Qi W, Hou S (1997): Immunohistochemical study on the distribution and coexistence of SP, VIP and NPY in the rat submandibular gland. Zhongguo Yi Xue Ke Xue Yuan Xue Bao 19, 107−109.
Kaleczyc Jerzy, Scheuermann Dietrich W., Timmermans Jean-Pierre, Majewski Mariusz, Lakomy Mirosław (1999): Immunohistochemical properties of nerve fibres supplying accessory male genital glands in the pig. A colocalisation study. Histochemistry and Cell Biology, 111, 217-228
Kawa K, Roper S (1984): On the two subdivisions and intrinsic synaptic connexions in the submandibular ganglion of the rat.. The Journal of Physiology, 346, 301-320
Klimaschewski Lars, Kummer Wolfgang, Heym Christine (1996): Localization, regulation and functions of neurotransmitters and neuromodulators in cervical sympathetic ganglia. Microscopy Research and Technique, 35, 44-68 doi:10.1002/(SICI)1097-0029(19960901)35:1<44::AID-JEMT5>3.0.CO;2-S
Kobashi Miyuki, Ichikawa Hiroyuki, Kobashi Motoi, Funahashi Makoto, Mitoh Yoshihiro, Matsuo Ryuji (2005): The origin of sensory nerve fibers that innervate the submandibular salivary gland in the rat. Brain Research, 1060, 184-187
Konopka L. M., May V., Forehand C. J. (1992): Galanin-like innervation of rat submandibular and sublingual salivary glands: Origin and effect on acinar cell membranes. The Journal of Comparative Neurology, 317, 271-282
Kuci Z, Seitz G, Kuci S, Kreyenberg H, Schumm M, Lang P, Niethammer D, Handgretinger R, Bruchelt G (2006): Pitfalls in detection of contaminating neuroblastoma cells by tyrosine hydroxylase RT-PCR due to catecholamine-producing hematopoietic cells. Anticancer Research 26, 2075−2080.
Kusakabe Tatsumi, Matsuda Hideki, Kawakami Tadashi, Syoui Noriko, Kurihara Katsuyoshi, Tsukuda Mamoru, Takenaka Toshifumi (1997): Distribution of neuropeptide-containing nerve fibers in the human submandibular gland, with special reference to the difference between serous and mucous acini. Cell and Tissue Research, 288, 25-31
Lai Jian-Ping, Douglas Steven D, Ho Wen-Zhe (1998): Human lymphocytes express substance P and its receptor. Journal of Neuroimmunology, 86, 80-86
Lohinai Zs., Székely A.D., Soós L., Fehér E. (1995): Distribution of nitric oxide synthase containing elements in the feline submandibular gland. Neuroscience Letters, 192, 9-12
Ng YK, Wong WC, Ling EA (1995): A study of the structure and functions of the submandibular ganglion. Annals Academy of Medicine, Singapore 24, 793−801.
Norberg KA, Olson L (1965): Adrenergic innervation of the salivary glands in the rat. Zeitschrift fur Zellforschung und Mikroskopische Anatomie 68, 183−189.
Proctor GB, Carpenter GH (2007): Regulation of salivary gland function by autonomic nerves. Autonomic Neuroscience 30, 3−18.
Rossoni R.B., Machado A.B.M., Machado C.R.S. (1992): Autonomic Innervation of the Salivary Glands in Cebid Monkeys: A Histochemical Study. Cells Tissues Organs, 143, 211-218
Salo A., Ylikoski J., Uusitalo H. (1993): Distribution of calcitonin gene-related peptide immunoreactive nerve fibers in the human submandibular gland. Neuroscience Letters, 150, 137-140
Schafer MK, Eiden LE, Weihe E (1998): Cholinergic neurons and terminal fields revealed by immunohistochemistry for the vesicular acetylcholine transporter. II. The peripheral nervous system. Neuroscience 84, 361−376.
Schultz T., Soinila J., Tolonen R., H�pp�l� O., Uusitalo H., Salo A. (1994): The sympathetic and parasympathetic nature of neuropeptide Y-immunoreactive nerve fibres in the major salivary glands of the rat. The Histochemical Journal, 26, 563-570
Shida Toru, Ueda Yutaka, Ishida-Yamamoto Akemi, Senba Emiko, Tohyama Masaya (1991): Enkephalinergic sympathetic and parasympathetic innervation of the rat submandibular and sublingual glands. Brain Research, 555, 288-294
Soinila J., Salo A., Uusitalo H., Yanaihara N., H�pp�l� O. (1989): CGRP-immunoreactive sensory nerve fibers in the submandibular gland of the rat. Histochemistry, 91, 455-460
Soinila J, Happola O, Yanaihara N, Soinila S (1991a): Immunohistochemical localization of [Met5]encephalin and [Met5]enkephalin-Arg6-Gly7-Leu8 in sympathetic and parasympathetic neurons and nerve fibers projecting to the rat submandibular gland. Neuroscience 40, 545−554.
Soinila J, Salo A, Uusitalo H, Soinila S, Yanaihara N, Happola O (1991b): Met5-enkephalin-Arg6-Gly7-Leu8-immunoreactive nerve fibers in the major salivary glands of the rat: evidence for both sympathetic and parasympathetic origin. Cell and Tissue Research 264, 15−22.
Soinila Jouni, Nuorva Kyösti, Soinila Seppo (2006): Nitric oxide synthase in human salivary glands. Histochemistry and Cell Biology, 125, 717-723
Stembirek J., Kyllar M., Putnova I., Stehlik L., Buchtova M. (2012): The pig as an experimental model for clinical craniofacial research. Laboratory Animals, 46, 269-279
Suzuki T, Sakada S (1972): Synaptic transmission in the submandibular ganglion of the rat. The Bulletin of Tokyo Dental College 13, 145−164.
Swindle M. M., Makin A., Herron A. J., Clubb F. J., Frazier K. S. (): Swine as Models in Biomedical Research and Toxicology Testing. Veterinary Pathology, 49, 344-356
Takai Noriyasu, Uchihashi Kenji, Higuchi Kyoko, Yoshida Yo, Yamaguchi Masaki (1999): Localization of neuronal-constitutive nitric oxide synthase and secretory regulation by nitric oxide in the rat submandibular and sublingual glands. Archives of Oral Biology, 44, 745-750
Tobin G., Luts A., Sundler F., Ekström J. (1990): Peptidergic innervation of the major salivary glands of the ferret. Peptides, 11, 863-867
Tobin G., Asztély A., Edwards A.V., Ekström J., Håkanson R., Sundler F. (1995): Presence and effects of pituitary adenylate cyclase activating peptide in the submandibular gland of the ferret. Neuroscience, 66, 227-235
Tosios Konstantinos I., Nikolakis Michail, Prigkos Andreas Christoforos, Diamanti Smaragda, Sklavounou Alexandra (2010): Nerve cell bodies and small ganglia in the connective tissue stroma of human submandibular glands. Neuroscience Letters, 475, 53-55
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