Effect of pre-synchronisation with progestogen and eCG on reproductive activity in synchronised ewes during anoestrous season

https://doi.org/10.17221/244/2020-VETMEDCitation:

Guner B, Kulaksiz R, Saat N, Kisadere I, Ozturkler M, Dalginli KY, Pancarci SM (2022): Effect of pre-synchronisation with progestogen and eCG on reproductive activity in synchronised ewes during anoestrous season. Vet Med-Czech 67, 231–239.

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The objective of the study was to investigate the effect of pre-synchronisation on the occurrence of the oestrus and pregnancy rate after fixed time artificial insemination (FTAI) in synchronised ewes during the anoestrous season. Kivircik ewes (n = 84) were randomly assigned to one of the two treatment groups with (PRE; n = 42) or without (SYN; n = 42) pre-synchronisation. In the SYN group, the ewes were subjected to a 7-d short-term protocol (P4 insertion-6d-PGF2α-1d-P4 removal + eCG). In the PRE group, the same short-term protocol as in the SYN group was applied  with 7 days apart for a pre-synchronised synchronisation protocol. A cervical FTAI was performed with fresh semen at 54 h after sponge removal. At the beginning of the synchronisation protocol, the oestrous response (66.7% vs. 0.0%) and cyclicity rates (64.3% vs. 14.3%) based on progesterone (P4) were higher in the PRE group compared to those in the SYN group, respectively (< 0.01). However, the oestrous response after synchronisation was lower within 96 h (57.1% vs. 95.2%; < 0.01) in the PRE group compared to that in the SYN group. Although the pregnancy rate after the FTAI was significantly (< 0.05) lower in the PRE group (14.3%) than the SYN group (35.7%), the overall pregnancy rate after natural mating was not different (95.2%) between the groups. In conclusion, the pre-synchronisation decreased the oestrous response leading to a lower pregnancy rate after the FTAI in the synchronised ewes during the anoestrous season. Thus, attention should be paid to two consecutive administrations of eCG in a pre-synchronisation and synchronisation protocol in ewes.

References:
Abecia JA, Forcada F, Gonzalez-Bulnes A. Hormonal control of reproduction in small ruminants. Anim Reprod Sci. 2012 Feb;130(3-4):173-9. https://doi.org/10.1016/j.anireprosci.2012.01.011
 
Baril G, Leboeuf B, Saumande J. Synchronization of estrus in goats: The relationship between time of occurrence of estrus and fertility following artificial insemination. Theriogenology. 1993 Sep;40(3):621-8. https://doi.org/10.1016/0093-691X(93)90414-Z
 
Biehl MV, de Ferraz Junior MVC, Barroso JPR, Susin I, Ferreira EM, Polizel DM, Pires AV. The reused progesterone device has the same effect on short or long estrus synchronization protocols in tropical sheep. Trop Anim Health Prod. 2019 Jul;51(6):1545-9. https://doi.org/10.1007/s11250-019-01841-1
 
Blaschi W, Lunardelli PA, Marinho LS, Max MC, Santos GM, Silva-Santos KC, Melo-Sterza FA, Baldassarre H, Rigo TR, Seneda MM. Effects of progestagen exposure duration on estrus synchronization and conception rates of crossbreed ewes undergoing fixed time artificial insemination. J Vet Sci. 2014;15(3):433-7. https://doi.org/10.4142/jvs.2014.15.3.433
 
Bodin L, Drion PV, Remy B, Brice G, Cognie Y, Beckers JF. Anti-PMSG antibody levels in sheep subjected annually to oestrus synchronisation. Reprod Nutr Dev. 1997 Nov-Dec;37(6):651-60. https://doi.org/10.1051/rnd:19970604
 
Casali R, Pinczak A, Cuadro F, Guillen-Munoz JM, Mezzalira A, Menchaca A. Semen deposition by cervical, transcervical and intrauterine route for fixed-time artificial insemination (FTAI) in the ewe. Theriogenology. 2017 Nov;103:30-5. https://doi.org/10.1016/j.theriogenology.2017.07.021
 
Cosentino IO, Balaro MFA, Arashiro EKN, Santos JDR, Carvalho ABDS, Clariget RP, Ungerfeld R, Brandao FZ. Hormonal protocols for early resynchronization of ovulation in ewes: The use of progestagens, eCG, and inclusion of early pregnancy diagnosis with color Doppler ultrasound. Theriogenology. 2019 Jul 15;133:113-8. https://doi.org/10.1016/j.theriogenology.2019.04.033
 
Das GK, Naqvi SM, Gulyani R, Pareek SR, Mittal JP. Effect of two doses of progesterone on estrus response and fertility in acycling crossbred Bharat Merino ewes in a semi-arid tropical environment. Small Rumin Res. 2000 Jul 1;37(1-2):159-63. https://doi.org/10.1016/S0921-4488(99)00151-0
 
Dirandeh E. Starting Ovsynch protocol on day 6 of first postpartum estrous cycle increased fertility in dairy cows by affecting ovarian response during heat stress. Anim Reprod Sci. 2014 Oct;149(3-4):135-40. https://doi.org/10.1016/j.anireprosci.2014.07.018
 
Dogan I, Nur Z. Different estrous induction methods during the non-breeding season in Kivircik ewes. Vet Med-Czech. 2006 Apr;51(4):133-8. https://doi.org/10.17221/5532-VETMED
 
Dogan I, Nur Z, Kilinc B. Different estrus induction protocols and fixed time artificial insemination during the anoestrous period in non-lactating Kivircik ewes. J Hell Vet Med Soc. 2018 Mar;69(1):801-8. https://doi.org/10.12681/jhvms.16429
 
Gibbons AE, Fernandez J, Bruno-Galarraga MM, Spinelli MV, Cueto MI. Technical recommendations for artificial insemination in sheep. Anim Reprod. 2019 Nov 18;16(4):803-9. https://doi.org/10.21451/1984-3143-AR2018-0129
 
Gumen A, Keskin A, Yilmazbas-Mecitoglu G, Karakaya E, Alkan A, Okut H, Wiltbank MC. Effect of presynchronization strategy before Ovsynch on fertility at first service in lactating dairy cows. Theriogenology. 2012 Nov;78(8):1830-8. https://doi.org/10.1016/j.theriogenology.2012.07.021
 
Herlihy MM, Giordano JO, Souza AH, Ayres H, Ferreira RM, Keskin A, Nascimento AB, Guenther JN, Gaska JM, Kacuba SJ, Crowe MA, Butler ST, Wiltbank MC. Presynchronization with double-Ovsynch improves fertility at first postpartum artificial insemination in lactating dairy cows. J Dairy Sci. 2012 Dec;95(12):7003-14. https://doi.org/10.3168/jds.2011-5260
 
Kaya S, Kacar C, Kaya D, Aslan S. The effectiveness of supplemental administration of progesterone with GnRH, hCG and PGF2α on the fertility of Tuj sheep during the non-breeding season. Small Rumin Res. 2013 Jul;113(2-3):365-70. https://doi.org/10.1016/j.smallrumres.2013.03.018
 
Kenyon PR, Maloney SK, Blache D. Review of sheep body condition score in relation to production characteristics. New Zeal J Agric Res. 2014 Jun;57(1):38-64. https://doi.org/10.1080/00288233.2013.857698
 
Martinez-Ros P, Lozano M, Hernandez F, Tirado A, Rios-Abellan A, Lopez-Mendoza MC, Gonzalez-Bulnes A. Intravaginal device-type and treatment-length for ovine estrus synchronization modify vaginal mucus and microbiota and affect fertility. Animals (Basel). 2018 Nov 29;8(12):1-8. https://doi.org/10.3390/ani8120226
 
Masoudi R, Zare Shahneh A, Towhidi A, Kohram H, Akbarisharif A, Sharafi M. Fertility response of artificial insemination methods in sheep with fresh and frozen-thawed semen. Cryobiology. 2017 Feb;74:77-80. https://doi.org/10.1016/j.cryobiol.2016.11.012
 
Menchaca A, Rubianes E. New treatments associated with timed artificial insemination in small ruminants. Reprod Fertil Dev. 2004;16(4):403-13. https://doi.org/10.1071/RD04037
 
Miranda VO, Oliveira FC, Dias JH, Vargas Junior SF, Goularte KL, Sa Filho MF, Sa Filho OG, Baldassarre H, Vieira AD, Lucia T Jr, Gasperin BG. Estrus resynchronization in ewes with unknown pregnancy status. Theriogenology. 2018 Jan 15;106:103-7. https://doi.org/10.1016/j.theriogenology.2017.10.019
 
Ozyurtlu N, Ay SS, Kucukaslan I, Gungor O, Aslan S. Effect of subsequent two short-term, short-term, and long-term progestagen treatments on fertility of Awassi ewes out of the breeding season. Ankara Üniv Vet Fak Derg. 2011 Jan;58(2):105-9. https://doi.org/10.1501/Vetfak_0000002458
 
Richardson BN, Hill SL, Stevenson JS, Djira GD, Perry GA. Expression of estrus before fixed-time AI affects conception rates and factors that impact expression of estrus and the repeatability of expression of estrus in sequential breeding seasons. Anim Reprod Sci. 2016 Mar;166:133-40. https://doi.org/10.1016/j.anireprosci.2016.01.013
 
Rosasco SL, Beard JK, Hallford DM, Summers AF. Evaluation of estrous synchronization protocols on ewe reproductive efficiency and profitability. Anim Reprod Sci. 2019 Nov;210:1-6. https://doi.org/10.1016/j.anireprosci.2019.106191
 
Roy F, Combes B, Vaiman D, Cribiu EP, Pobel T, Deletang F, Combarnous Y, Guillou F, Maurel MC. Humoral immune response to equine chorionic gonadotropin in ewes: Association with major histocompatibility complex and interference with subsequent fertility. Biol Reprod. 1999a Jul;61(1):209-18. https://doi.org/10.1095/biolreprod61.1.209
 
Roy F, Maurel MC, Combes B, Vaiman D, Cribiu EP, Lantier I, Pobel T, Deletang F, Combarnous Y, Guillou F. The negative effect of repeated equine chorionic gonadotropin treatment on subsequent fertility in Alpine goats is due to a humoral immune response involving the major histocompatibility complex. Biol Reprod. 1999b Apr;60(4):805-13. https://doi.org/10.1095/biolreprod60.4.805
 
Scaramuzzi RJ, Campbell BK, Downing JA, Kendall NR, Khalid M, Munoz-Gutierrez M, Somchit A. A review of the effects of supplementary nutrition in the ewe on the concentrations of reproductive and metabolic hormones and the mechanisms that regulate folliculogenesis and ovulation rate. Reprod Nutr Dev. 2006 Jul-Aug;46(4):339-54. https://doi.org/10.1051/rnd:2006016
 
Titi HH, Kridli RT, Alnimer MA. Estrus synchronization in sheep and goats using combinations of GnRH, progestagen and prostaglandin F2alpha. Reprod Domest Anim. 2010 Aug;45(4):594-9.
 
Tsakmakidis IA. Ram semen evaluation: Development and efficiency of modern techniques. Small Rumin Res. 2010 Aug;92(1-3):126-30. https://doi.org/10.1016/j.smallrumres.2010.04.017
 
Ungerfeld R, Rubianes E. Short term primings with different progestogen intravaginal devices (MAP, FGA and CIDR) for eCG-estrous induction in anestrus ewes. Small Rumin Res. 2002 Oct;46(1):63-6. https://doi.org/10.1016/S0921-4488(02)00105-0
 
Ustuner B, Gunay U, Nur Z, Ustuner H. Effects of long and short-term progestagen treatments combined with pmsg on oestrus synchronization and fertility in awassi ewes during the breeding season. Acta Vet Brno. 2007 Jul;76(3):391-7. https://doi.org/10.2754/avb200776030391
 
Vinoles C, Meikle A, Forsberg M, Rubianes E. The effect of subluteal levels of exogenous progesterone on follicular dynamics and endocrine patterns during early luteal phase of the ewe. Theriogenology. 1999 May;51(7):1351-61. https://doi.org/10.1016/S0093-691X(99)00079-5
 
Vinoles C, Forsberg M, Banchero G, Rubianes E. Effect of long-term and short-term progestagen treatment on follicular development and pregnancy rate in cyclic ewes. Theriogenology. 2001 Mar 1;55(4):993-1004. https://doi.org/10.1016/S0093-691X(01)00460-5
 
Yuthasastrakosol P, Palmer WM, Howland BE. Luteinizing hormone, oestrogen and progesterone levels in peripheral serum of anoestrous and cyclic ewes as determined by radioimmunoassay. J Reprod Fertil. 1975 Apr;43(1):57-65. https://doi.org/10.1530/jrf.0.0430057
 
Zeleke M, Greyling JPC, Schwalbach LMJ, Muller T, Erasmus JA. Effect of progestagen and PMSG on oestrous synchronization and fertility in Dorper ewes during the transition period. Small Rumin Res. 2005 Jan;56(1-3):47-53. https://doi.org/10.1016/j.smallrumres.2003.12.006
 
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