Effects of epididymis cold storage on frozen-thawed epididymal sperm quality in tomcats (Felis catus)

https://doi.org/10.17221/253/2015-VETMEDCitation:Perez-Marin C.C., Jimenez E., Aguera E.I. (2017): Effects of epididymis cold storage on frozen-thawed epididymal sperm quality in tomcats (Felis catus). Veterinarni Medicina, 62: 147-152.
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The effect of cold storage of testes and epididymides at 4 °C for 12 h on the cryopreservation capacity of epididymal feline sperm was evaluated. Ten domestic cats were castrated, and testes and epididymides collected. Specimens were randomly assigned to two groups: in Group A, epididymal samples were immediately processed and frozen in 0.25-ml straws; in Group B, both testes and epididymides were maintained in saline at 4 °C for 12 h and sperm was then processed and frozen. Motility, morphology, acrosome status, sperm viability and DNA integrity were assessed in epididymal sperm samples before freezing (baseline), at thawing (0 h) and 6 h post-thawing (6 h). Although values were lower in Group B, no significant intergroup difference was observed for any of the parameters tested either at baseline or at 0 h. However, significantly higher values (P < 0.05) were observed in Group A at 6 h for total sperm motility (29.0 ± 2.4% vs 13.0 ± 4.3%), sperm viability (35.2 ± 5.4% vs 15.4 ± 1.4%) and normal morphology (47.6 ± 0.8% vs 40.0 ± 2.1%). It was observed that motility and acrosome status of epididymal sperm are the most sensitive parameters when both types of sperm samples (from fresh epididymis or from 12 h cold-stored epididymis) are frozen-thawed. When sperm quality was assessed 6 h after thawing, spermatozoa precooled in the epididymides showed significantly lower values for motility, viability and morphology than spermatozoa from fresh epididymal samples.

References:
Axnér Eva, Hermansson Ulrika, Linde-Forsberg Catharina (2004): The effect of Equex STM paste and sperm morphology on post-thaw survival of cat epididymal spermatozoa. Animal Reproduction Science, 84, 179-191  https://doi.org/10.1016/j.anireprosci.2003.11.003
 
Baumber Julie, Ball Barry A., Linfor Jennifer J., Meyers Stuart A. (2003): Reactive Oxygen Species and Cryopreservation Promote DNA Fragmentation in Equine Spermatozoa. Journal of Andrology, 24, 621-628  https://doi.org/10.1002/j.1939-4640.2003.tb02714.x
 
Chatdarong K, Boonyen T, Rungsuk K, Chowtrakul C, Lohachit C (2006): Freezing of cat epididymal spermatozoa after cold storage. In: Proceedings of the 2nd symposium of the Asian zoo and wildlife medicine and the 1st workshop on the Asian zoo and wildlife pathology, Bangkok, Thailand. 20 pp.
 
Chatdarong K, Thuwanut P, Suksamai P, Patanatiradaj S, Sangwornrachasup A (2009): Survival of Frozen-Thawed Cat Spermatozoa Pre-Cooled in the Epididymides. Reproduction in Domestic Animals, 44, 377-380  https://doi.org/10.1111/j.1439-0531.2009.01412.x
 
Filliers M., Rijsselaere T., Bossaert P., De Causmaecker V., Dewulf J., Pope C.E., Van Soom A. (2008): Computer-assisted sperm analysis of fresh epididymal cat spermatozoa and the impact of cool storage (4°C) on sperm quality. Theriogenology, 70, 1550-1559  https://doi.org/10.1016/j.theriogenology.2008.07.004
 
Gañán N., Gomendio M., Roldan E.R.S. (2009): Effect of storage of domestic cat (Felis catus) epididymides at 5°C on sperm quality and cryopreservation. Theriogenology, 72, 1268-1277  https://doi.org/10.1016/j.theriogenology.2009.07.023
 
Goodrowe K.L., Hay M. (1993): Characteristics and zona binding ability of FRESH and cooled domestic cat epididymal spermatozoa. Theriogenology, 40, 967-975  https://doi.org/10.1016/0093-691X(93)90365-C
 
Harris RF, Pope CE, Gomez MC, Leibo SP, Dresser BL (2001): Storage of domestic cat spermatozoa for extended periods at 4 °C. Theriogenology 55, 308.
 
Hay MA, Goodrowe KL (1993): Comparative cryopreservation and capacitation of spermatozoa from epiddidymis and vasa deferentia of domestic cat. Journal of Reproduction and Fertility 47, 297–305.
 
Howard JG, Brown JL, Bush M, Wildt DE (1990): Teratospermic and normospermic domestic cats: ejaculate traits, pituitary-gonadal hormones, and improvement of spermatozoal motility and morphology after swim-up processing. Journal of Andrology 11, 204–215.
 
Kishikawa H., Tateno H., Yanagimachi R. (1999): Fertility of mouse spermatozoa retrieved from cadavers and maintained at 4 C. Reproduction, 116, 217-222  https://doi.org/10.1530/jrf.0.1160217
 
Soler A.J., Esteso M.C., Fernández-Santos M.R., Garde J.J. (2005): Characteristics of Iberian red deer (Cervus elaphus hispanicus) spermatozoa cryopreserved after storage at 5°C in the epididymis for several days. Theriogenology, 64, 1503-1517  https://doi.org/10.1016/j.theriogenology.2005.03.013
 
Thuwanut P, Chatdarong K (2012): Cryopreservation of Cat Testicular Tissues: Effects of Storage Temperature, Freezing Protocols and Cryoprotective Agents. Reproduction in Domestic Animals, 47, 777-781  https://doi.org/10.1111/j.1439-0531.2011.01967.x
 
Thuwanut P., Chatdarong K., Techakumphu M., Axnér E. (2008): The effect of antioxidants on motility, viability, acrosome integrity and DNA integrity of frozen-thawed epididymal cat spermatozoa. Theriogenology, 70, 233-240  https://doi.org/10.1016/j.theriogenology.2008.04.005
 
TOYONAGA Mari, SATO Yuuka, MORITA Miki, WATANABE Masashi, OBA Hiromichi, MIZUTANI Tatsuji, HORI Tatsuya, TSUTSUI Toshihiko (2010): The Qualities of Cryopreserved Epididymal Sperm Collected from Feline Epididymides Stored at Low Temperature. Journal of Veterinary Medical Science, 72, 777-780  https://doi.org/10.1292/jvms.09-0260
 
Toyonaga Mari, Sato Yuuka, Sasaki Ayako, Kaihara Aya, Tsutsui Toshihiko (2011): Artificial insemination with cryopreserved sperm from feline epididymides stored at 4 °C. Theriogenology, 76, 532-537  https://doi.org/10.1016/j.theriogenology.2011.03.005
 
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