Age and sex-related differences in the haematological parameters of captive African grey parrots (Psittacus erithacus)

https://doi.org/10.17221/31/2020-VETMEDCitation:

Gaspar H, Bargallo F, Grifols J, Correia E, Pinto ML (2021): Age and sex-related differences in the haematological parameters of captive African grey parrots (Psittacus erithacus). Vet Med-Czech 66, 330–341.

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African grey parrots (Psittacus erithacus) are very popular pets, commonly seen in avian clinical practice. Haematological profiles are critical to the understanding of several disease processes, being particularly useful as diagnostic tools in clinical practice, since birds tend to hide clinical signs of disease. We have previously proposed new haematological reference intervals (RI) for captive African grey parrots, and in the present work the basic data obtained was studied in detail to investigate the influence of factors, such as age and sex, on the haematological profile of this bird species. During an 8-year period (March 2009 to July 2017), animals (n = 239) examined in first consultations or check-ups at the Zoològic Veterinaris (Barcelona) were submitted to blood collection at different time points, rendering a total of 459 blood samples. The haematological testing was performed according to the guidelines of the American Society of Veterinary Clinical Pathology to determine the packed cell volume (PCV), haemoglobin (Hb), mean haemoglobin concentration (MHC), mean corpuscular volume (MCV), mean corpuscular haemoglobin concentration (MCHC), total erythrocyte count (TRBC), total leukocyte count (TWBC), and differential leukogram with absolute and relative counts. All the haematological testing was performed in an in-house laboratory as previously described. Animals with 0 to 4 years of age showed higher values of PCV (P < 0.001), Hb (P = 0.023) and RBC (P = 0.018), and lower values of MCHC (P = 0.008), WBC (P = 0.012) and heterophils (P < 0.001) than older animals. There were significant differences exhibited in the monocytes (P = 0.035) between different age groups. Females presented higher PCV, Hb and RBC values (P < 0.001) compared to males. Our results suggest that the age and sex influence the haematological parameters in a significant manner in African grey parrots and should be accounted for when assessing the health status of individuals from this species.

References:
Allgayer MC, Guedes NM, Chiminazzo C, Cziulik M, Weimer TA. Clinical pathology and parasitologic evaluation of free-living nestlings of the Hyacinth Macaw (Anodorhynchus hyacinthinus). J Wildl Dis. 2009 Oct;45(4):972-81. https://doi.org/10.7589/0090-3558-45.4.972
 
Annorbah NND, Collar NJ, Marsden SJ. Trade and habitat change virtually eliminate the grey parrot (Psittacus erithacus) from Ghana. Ibis. 2016;158(1):82-91. https://doi.org/10.1111/ibi.12332
 
Bellwood B, Andrasik-Catton M. Blood analysis. In: Bellwood B, Andrasik-Cratton M, editors. Veterinary technician’s handbook of laboratory procedures. Oxford, UK: Wiley-Blackwell; 2014. p. 17-52.
 
Berkunsky I, Quillfeldt P, Brightsmith DJ, Abbud MC, Aguilar JMRE, Aleman-Zelaya U et al. Current threats faced by Neotropical parrot populations. Biol Conserv. 2017 Oct;214:278-87. https://doi.org/10.1016/j.biocon.2017.08.016
 
Best JR. Handling. In: Harcourt-Brown NH, Chitty J, editors. BSAVA manual of psittacine birds. Ames, Iowa, USA: British Small Animal Veterinary Association; 2005. p. 31-4.
 
BirdLife International. Psittacus erithacus: The IUCN red list of threatened species; 2018. e.T22724813A129879439 [Internet]. 2018 [cited 2020 Sep 1]. Available from: dx.doi.org/10.2305/IUCN.UK.2018-2.RLTS.T22724813A129879439.en.
 
Campbell TW. Hematology. In: Ritchie BW, Harrison GJ, Harrison LR, editors. Avian medicine: Principles and application. Lake Worth, Florida: Winger; 1994. p. 176-98.
 
Campbell TW. Hematology of psittacines. In: Weiss DJ, Wardrop KJ, editors. Schalm’s veterinary hematology. Oxford, UK: Wiley-Blackwell; 2010. p. 968-76.
 
Campbell TW. Blood sample collection and preparation in birds. In: Campbell TW, editor. Exotic animal hematology and cytology. Oxford, UK: Wiley-Blackwell; 2015a. p. 165-72.
 
Campbell TW. Evaluation of the blood film. Vet Clin North Am Exot Anim Pract. 2015b Jan;18(1):117-35. https://doi.org/10.1016/j.cvex.2014.09.001
 
Campbell TW. Hematologic techniques in lower vertebrates. In: Campbell TW, editor. Exotic animal hematology and cytology. Oxford, UK: Wiley-Blackwell; 2015c. p. 199-205.
 
Chitty J. Basic techniques. In: Harcourt-Brown NH, Chitty J, editors. BSAVA manual of psittacine birds. Ames, Iowa, USA: British Small Animal Veterinary Association; 2005. p. 50-9.
 
Clark P, Boardman WSJ, Raidal SR. Collection and handling blood samples. In: Clark P, Boardman WSJ, Raidal SR, editors. Atlas of clinical avian hematology. Oxford, UK: Wiley-Blackwell; 2009. p. 1-32.
 
Clubb SL, Schubot RM, Joyner K, Zinkl JG, Wolf S, Escobar J, Clubb KJ, Kabbur MB. Hematologic and serum biochemical reference intervals in juvenile eclectus parrots (Eclectus roratus). J Ass Avian Vet. 1990;4(4):218-25. https://doi.org/10.2307/27670971
 
Clubb SL, Schubot RM, Joyner K, Zinkl JG, Wolf S, Escobar J, Clubb KJ, Kabbur MB. Hematologic and serum biochemical reference intervals in juvenile macaws (Ara sp.). J Ass Avian Vet. 1991a;5(3):154-62. https://doi.org/10.2307/27671042
 
Clubb SL, Schubot RM, Joyner K, Zinkl JG, Wolf S, Escobar J, Clubb KJ, Kabbur MB. Hematologic and serum biochemical reference intervals in juvenile cockatoos. J Ass Avian Vet. 1991b;5(1):16-26. https://doi.org/10.2307/27671000
 
Coles BH. Species and natural history. In: Harcourt-Brown NH, Chitty J, editors. BSAVA manual of psittacine birds. Ames, Iowa, USA: British Small Animal Veterinary Association; 2005. p. 1-6.
 
Cornejo J, Richardson D, Perez JG, Brightsmith DJ, Bonar CJ, Heatley JJ. Hematologic and plasma biochemical reference values of the horned guan, Oreophasis derbianus. J Zoo Wildl Med. 2014 Mar;45(1):15-22. https://doi.org/10.1638/2011-0278R1.1
 
Davis AK, Maney DL, Maerz JC. The use of leukocyte profiles to measure stress in vertebrates: A review for ecologists. Func Ecol. 2008;22(5):760-72. https://doi.org/10.1111/j.1365-2435.2008.01467.x
 
Davis AK. Effect of handling time and repeated sampling on avian white blood cell counts. J Field Ornithol. 2005;76(4):334-8. https://doi.org/10.1648/0273-8570-76.4.334
 
Dolka B, Wodarczyk R, Zbikowski A, Dolka I, Szeleszczuk P, Klucinski W. Haematological parameters in relation to age, sex and biochemical values for mute swans (Cygnus olor). Vet Res Commun. 2014 Jun;38(2):93-100. https://doi.org/10.1007/s11259-014-9589-y
 
Doneley B. Clinical techniques. In: Doneley B, editor. Avian medicine and surgery in practice: Companion and aviary birds. Boca Raton, Florida, USA: CRC Press; 2016. p. 81-94.
 
Doussang D, Merino V, Moreno L, Islas A, Barrientos C, Mathieu C, Cerda F, Lopez J, Ortega R, Gonzalez-Acuna D. Hematologic and biochemical parameters of kelp gulls (Larus dominicanus) captured in the city of Talcahuano, Chile. J Zoo Wildl Med. 2015 Sep;46(3):447-55. https://doi.org/10.1638/2012-0080.1
 
Dujowich M, Mazet JK, Zuba JR. Hematologic and biochemical reference ranges for captive California condors (Gymnogyps californianus). J Zoo Wildl Med. 2005 Dec;36(4):590-7. https://doi.org/10.1638/04-111.1
 
Ewenson EL, Zann RA, Flannery GR. Body condition and immune response in wild zebra finches: Effects of capture, confinement and captive-rearing. Naturwissenschaften. 2001 Sep;88(9):391-4. https://doi.org/10.1007/s001140100250
 
Foldenauer U, Borjal RJ, Deb A, Arif A, Taha AS, Watson RW, Steinmetz H, Burkle M, Hammer S. Hematologic and plasma biochemical values of Spix’s macaws (Cyanopsitta spixii). J Avian Med Surg. 2007 Dec;21(4):275-82. https://doi.org/10.1647/2007-004R.1
 
Fourie FLR, Hattinch J. Comparative haemotology of some south african birds. Comp Biochem Physiol. 1983;74A(2):443-8. https://doi.org/10.1016/0300-9629(83)90628-X
 
Friedrichs KR, Harr KE, Freeman KP, Szladovits B, Walton RM, Barnhart KF, Blanco-Chavez J; American Society for Veterinary Clinical Pathology. ASVCP reference interval guidelines: Determination of de novo reference intervals in veterinary species and other related topics. Vet Clin Pathol. 2012 Dec;41(4):441-53. https://doi.org/10.1111/vcp.12006
 
Fudge AM. Avian laboratory medicine. In: Fudge AM, editor. Laboratory medicine: Avian and exotic pets. Philadelphia, USA: Saunders; 2000a. p. 1-8.
 
Fudge AM. Laboratory reference ranges for selected avian, mammalian, and reptilian species. In: Fudge AM, editor. Laboratory medicine: Avian and exotic pets. Philadelphia, USA: Saunders; 2000b. p. 376-99.
 
Gaspar H, Bargallo F, Grifols J, Correia E, Pinto ML. Haematological reference intervals in captive African grey parrots (Psittacus erithacus). Vet Med-Czech. 2021;66(1):24-31. https://doi.org/10.17221/15/2020-VETMED
 
Gayathri KL, Hegde SN. Sexual differences in blood values of the pigeon, Columba livia. Comp Biochem Physiol B. 1994;109(2-3):219-24. https://doi.org/10.1016/0305-0491(94)90005-1
 
Haefele HJ, Sidor I, Evers DC, Hoyt DE, Pokras MA. Hematologic and physiologic reference ranges for free-ranging adult and young common loons (Gavia immer). J Zoo Wildl Med. 2005 Sep;36(3):385-90. https://doi.org/10.1638/03-018.1
 
Han JI, Jang HJ, Na KJ. Hematologic and serum biochemical reference intervals of the Oriental white stork (Ciconia boyciana) and the application of an automatic hematologic analyzer. J Vet Sci. 2016 Sep 30;17(3):399-405. https://doi.org/10.4142/jvs.2016.17.3.399
 
Harper EJ, Lowe B. Hematology values in a colony of budgerigars (Melopsittacus undulatus) and changes associated with aging. J Nutr. 1998 Dec;128(12 Suppl):2639S-40S. https://doi.org/10.1093/jn/128.12.2639S
 
Hauptmanova K, Maly M, Literak I. Changes of hematological parameters in common pheasant throughout the year. Vet Med-Czech. 2006 Jan;51(1):29-34. https://doi.org/10.17221/5514-VETMED
 
Hawkey C, Hart MG, Samour HJ. Age-related haematological changes and haemopathological responses in Chilean flamingos (Phoenicopterus chiliensis). Avian Pathol. 1984 Apr;13(2):223-9. https://doi.org/10.1080/03079458408418526
 
Hernandez M, Margalida A. Hematology and blood chemistry reference values and age-related changes in wild bearded vultures (Gypaetus barbatus). J Wildl Dis. 2010 Apr;46(2):390-400. https://doi.org/10.7589/0090-3558-46.2.390
 
Howlett JC, Samour JH, Bailey TA, Naldo JL. Age-related haematology changes in captive reared kori bustards (Ardeotis kori). Comp Haem Int. 1998;8(1):26-30. https://doi.org/10.1007/BF02628101
 
Jones MP, Arheart KL, Cray C. Reference intervals, longitudinal analyses, and index of individuality of commonly measured laboratory variables in captive bald eagles (Haliaeetus leucocephalus). J Avian Med Surg. 2014 Jun;28(2):118-26. https://doi.org/10.1647/2013-001
 
Kalmbach E, Griffiths R, Crane JE, Furness RW. Effects of experimentally increased egg production on female body condition and laying dates in the great skua Stercorarius skua. J Avian Biol. 2004;35(6):501-14. https://doi.org/10.1111/j.0908-8857.2004.03271.x
 
Kocan RM, Pitts SM. Blood values of the canvasback duck by age, sex and season. J Wildl Dis. 1976 Jul;12(3):341-6. https://doi.org/10.7589/0090-3558-12.3.341
 
Kolesnikovas CK, Niemeyer C, Teixeira RH, Nunes AL, Rameh-de-Albuquerque LC, Sant’Anna SS, Catao-Dias JL. Hematologic and plasma biochemical values of hyacinth macaws (Anodorhynchus hyacinthinus). J Avian Med Surg. 2012 Sep;26(3):125-9. https://doi.org/10.1647/2011-020R1.1
 
Lanzarot MP, Barahona MV, Andres MI, Fernandez-Garcia M, Rodriguez C. Hematologic, protein electrophoresis, biochemistry, and cholinesterase values of free-living black stork nestlings (Ciconia nigra). J Wildl Dis. 2005 Apr;41(2):379-86. https://doi.org/10.7589/0090-3558-41.2.379
 
Latimer KS, Bienzle D. Determination and interpretation of the avian leukogram. In: Feldman BF, Zinkl JG, Jain NC, editors. Schalm’s veterinary hematology. Philadelphia: Lippincott Williams & Wilkins; 2000. p. 417-32.
 
Lee KA. Linking immune defenses and life history at the levels of the individual and the species. Integr Comp Biol. 2006 Dec;46(6):1000-15. https://doi.org/10.1093/icb/icl049
 
Lochmiller RL, Deerenberg C. Trade-offs in evolutionary immunology: Just what is the cost of immunity? Oikos. 2000;88(1):87-98. https://doi.org/10.1034/j.1600-0706.2000.880110.x
 
Marion CJ, Carpenter JW. Birds. In: Carpenter JW, editor. Exotic animal formulary. Philadelphia, USA: Saunders; 2013. p. 190-468.
 
Martin RO, Senni C, D’Cruze NC. Trade in wild-sourced African grey parrots: Insights via social media. Glob Ecol Conserv. 2018;15:e00429. https://doi.org/10.1016/j.gecco.2018.e00429
 
Martin RO. Grey areas: Temporal and geographical dynamics of international trade of Grey and Timneh Parrots (Psittacus erithacus and P. timneh) under CITES. Emu Austral Ornithol. 2018a;118(1):113-25. https://doi.org/10.1080/01584197.2017.1369854
 
Martin RO. The wild bird trade and African parrots: Past, present and future challenges. Ostrich. 2018b;89(2):139-43. https://doi.org/10.2989/00306525.2017.1397787
 
Masello JF, Choconi RG, Helmer M, Kremberg T, Lubjuhn T, Quillfeldt P. Do leucocytes reflect condition in nestling burrowing parrots Cyanoliseus patagonus in the wild? Comp Biochem Physiol A Mol Integr Physiol. 2009 Feb;152(2):176-81. https://doi.org/10.1016/j.cbpa.2008.09.018
 
Merino S, Martinez J, Moller AP, Sanabria L, de Lope F, Perez J, Rodriguez-Caabeiro F. Phytohaemagglutinin injection assay and physiological stress in nestling house martins. Anim Behav. 1999 Jul;58(1):219-22. https://doi.org/10.1006/anbe.1999.1127
 
Montolio SA, Molina Lopez R, Cray C, Lavin Gonzalez S, Nicolas Francisco O, Marco Sanchez I, Casas-Diaz E, Cuenca Valera R. Hematologic reference intervals and age effect in European Strigiformes. Vet Clin Pathol. 2017 Sep;46(3):483-95. https://doi.org/10.1111/vcp.12516
 
Nazifi S, Vesal N. Hematological values of healthy roseringed parakeets (Psittaculla krameri). J Appl Animal Res. 2003;24(2):165-8. https://doi.org/10.1080/09712119.2003.9706452
 
Norris K, Evans MR. Ecological immunology: Life history trade-offs and immune defense in birds. Behav Ecol. 2000 Jan;11(1):19-26. https://doi.org/10.1093/beheco/11.1.19
 
Ots I, Murumagi A, Horak P. Hematological health state indices of reproducing Great Tits: Methodology and sources of natural variation. Funct Ecol. 1998;12(4):700-7. https://doi.org/10.1046/j.1365-2435.1998.00219.x
 
Parsons NJ, Schaefer AM, van der Spuy SD, Gous TA. Establishment of baseline haematology and biochemistry parameters in wild adult African penguins (Spheniscus demersus). J S Afr Vet Assoc. 2015 Mar 25;86(1):e1-e8. https://doi.org/10.4102/jsava.v86i1.1198
 
Plischke A, Quillfeldt P, Lubjuhn T, Merino S, Masello JF. Leucocytes in adult burrowing parrots Cyanoliseus patagonus in the wild: Variation between contrasting breeding seasons, gender, and individual condition. J Ornithol. 2010;151(2):347-54. https://doi.org/10.1007/s10336-009-0461-8
 
Prioste FE, Zwarg T, Teixeira RH, Vanstreels RE, Rocha A, Matushima ER. Hematologic reference values for clinically healthy captive golden conures (Guaruba guarouba). Avian Dis. 2012 Dec;56(4):701-3. https://doi.org/10.1637/10225-043012-Reg.1
 
Roskov Y, Abucay L, Orrell T, Nicolson D, Bailly N, Kirk PM, Bourgoin T, DeWalt RE, Decock W, De Wever A, Niukerken E van, Zarucchi J, Penev L. Catalogue of life. Species 2000 ITIS Cat. Life [Internet]. 2017 [cited 2020 Nov 11]. Available from: www.catalogueoflife.org/col/browse/tree/id/67847d320573ff18b0384e22afe97ed8.
 
Samour J. Diagnostic value of hematology. In: Harrison GJ, Lightfoot TL, editors. Clinical avian medicine. Palm Beach, Florida, USA: Spix Pub; 2006. p. 587-610.
 
Schmidt EMS, Paulillo AC, Dittrich RL, Santin E, Silva PCL, Beltrame O, Oliveira EG. The effect of age on hematological and serum biochemical values on juvenile ring-necked pheasants (Phasianus colchicus). Int J Poultry Sci. 2007;6(6):459-61. https://doi.org/10.3923/ijps.2007.459.461
 
Schmidt EM, Lange RR, Ribas JM, Daciuk BM, Montiani-Ferreira F, Paulillo AC. Hematology of the Red-capped parrot (Pionopsitta pileata) and Vinaceous Amazon parrot (Amazona vinacea) in captivity. J Zoo Wildl Med. 2009 Mar;40(1):15-7. https://doi.org/10.1638/2007-0054.1
 
Tell LA, Citino SB. Hematologic and serum chemistry reference intervals for Cuban amazon parrots (Amazona leucocephala leucocephala). J Zoo Wild Med. 1992;23(1):62-4.
 
Tieleman BI, Williams JB, Ricklefs RE, Klasing KC. Constitutive innate immunity is a component of the pace-of-life syndrome in tropical birds. Proc Biol Sci. 2005 Aug 22;272(1573):1715-20. https://doi.org/10.1098/rspb.2005.3155
 
Tully Jr TN. Birds. In: Tully Jr TN, Mitchell MA, editors. Manual of exotic pet practice. Philadelphia, USA: Saunders; 2009. p. 250-98.
 
Vergneau-Grosset C, Polley T, Holt DC, Vernau W, Paul-Murphy J. Hematologic, plasma biochemical, and lipid panel reference intervals in orange-winged Amazon parrots (Amazona amazonica). J Avian Med Surg. 2016 Dec;30(4):335-44. https://doi.org/10.1647/2015-129
 
Villegas A, Guzman JMS, Corbacho C, Corbacho P, Vargas JM. Blood values of Bald Ibis (Geronticus eremita) in captivity: Comparative ranges and variability with age, sex and physical condition. J Ornithol. 2004;145(2):98-104. https://doi.org/10.1007/s10336-003-0016-3
 
Vleck CM, Vertalino N, Vleck D, Bucher TL. Stress, corticosterone, and heterophil to lymphocyte ratios in free-living Adelie Penguins. Condor. 2000;102(2):392-400. https://doi.org/10.1093/condor/102.2.392
 
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