Dietary Lactobacillus plantarum alleviated Salmonella Typhimurium infection and suppressed Jak/Stat pathway in rabbits

https://doi.org/10.17221/34/2021-VETMEDCitation:

Wen F, Liu X, Ding K, Zhang F, Wang J (2022): Dietary Lactobacillus plantarum alleviated Salmonella Typhimurium infection and suppressed Jak/Stat pathway in rabbits. Vet Med-Czech 67, 519–526.

download PDF

This study aimed to investigate the effect of a Lactobacillus plantarum DPP8 supplementation on the growth performance, pathogenic invasion, inflammation and pathogen resistance signal pathway in rabbits infected with Salmonella Typhimurium (S. Typhimurium). The treatments included a negative control, a positive control with an S. Typhimurium infection and a positive control plus DPP8 at 106, 108 or 1010 cfu/kg of diet using 300 weaned rabbits. The results showed that supplementing with DPP8 improved (P < 0.05) the feed intake, body weight gain and feed efficiency compared to the positive control. Also, DPP8 decreased (P < 0.05) the S. Typhimurium colonisation and translocation, serum IL-1β, IL-6 and TNF-α, and intestinal mucosa mRNA expressions of the inflammatory mediators Janus kinase (Jak) 2, the signal transducer and activator of transcription (Stat) 1 and 3 at 7 and 14 d post administration. The dose analysis of DPP8 showed linear increases (P ≤ 0.007) in the feed intake and body weight gain, but linear decreases (P ≤ 0.022) in the S. Typhimurium loads, IL-1β, IL-6 and Jak2. It is concluded that Lactobacillus plantarum DPP8 can be used as a supportive probiotic against an S. Typhimurium infection and it possibly plays a direct or indirect role in the downregulation of the Jak/Stat pathway in rabbits.

References:
Adhikari P, Lee CH, Cosby DE, Cox NA, Kim WK. Effect of probiotics on fecal excretion, colonization in internal organs and immune gene expression in the ileum of laying hens challenged with Salmonella Enteritidis. Poult Sci. 2019 Mar 1;98(3):1235-42. https://doi.org/10.3382/ps/pey443
 
An JM, Kang EA, Han YM, Oh JY, Lee DY, Choi SH, Kim DH, Hahm KB. Dietary intake of probiotic kimchi ameliorated IL-6-driven cancer cachexia. J Clin Biochem Nutr. 2019 Sep;65(2):109-17. https://doi.org/10.3164/jcbn.19-10
 
Bagarolli RA, Tobar N, Oliveira AG, Araujo TG, Carvalho BM, Rocha GZ, Vecina JF, Calisto K, Guadagnini D, Prada PO, Santos A, Saad STO, Saad MJA. Probiotics modulate gut microbiota and improve insulin sensitivity in DIO mice. J Nutr Biochem. 2017 Dec;50:16-25. https://doi.org/10.1016/j.jnutbio.2017.08.006
 
Coble DJ, Sandford EE, Ji T, Abernathy J, Fleming D, Zhao H, Lamont SJ. Impacts of Salmonella enteritidis infection on liver transcriptome in broilers. Genesis. 2013 May;51(5):357-64. https://doi.org/10.1002/dvg.22351
 
Deng Q, Shi H, Luo Y, Zhao H, Liu N. Effect of dietary Lactobacilli mixture on Listeria monocytogenes infection and virulence property in broilers. Poult Sci. 2020 Jul;99(7):3655-62. https://doi.org/10.1016/j.psj.2020.03.058
 
Deriu E, Liu JZ, Pezeshki M, Edwards RA, Ochoa RJ, Contreras H, Libby SJ, Fang FC, Raffatellu M. Probiotic bacteria reduce salmonella typhimurium intestinal colonization by competing for iron. Cell Host Microbe. 2013 Jul 17;14(1):26-37. https://doi.org/10.1016/j.chom.2013.06.007
 
Ding K, Jiang Q, Wang J, Liu N, Zhang F. Effect of tetramethylpyrazine on growth performance, Campylobacter jejuni carriage and endogenous antimicrobial peptides in rabbits. Czech J Anim Sci. 2019;64(11):465-71. https://doi.org/10.17221/138/2019-CJAS
 
Kanmani P, Kim H. Beneficial effect of immunobiotic strains on attenuation of Salmonella induced inflammatory response in human intestinal epithelial cells. PLoS One. 2020 Mar 9;15(3):e0229647. https://doi.org/10.1371/journal.pone.0229647
 
Kurganov BI. Kinetic regime of aggregation of UV-irradiated glyceraldehyde-3-phosphate dehydrogenase from rabbit skeletal muscle. Biochem Biophys Res Commun. 2018 Jan 1;495(1):1182-6. https://doi.org/10.1016/j.bbrc.2017.11.166
 
Liu N, Ding K, Wang JQ, Jia SC, Wang JP, Xu TS. Detoxification, metabolism, and glutathione pathway activity of aflatoxin B1 by dietary lactic acid bacteria in broiler chickens. J Anim Sci. 2017 Oct;95(10):4399-406. https://doi.org/10.2527/jas2017.1644
 
Liu N, Deng X, Liang C, Cai H. Effect of broccoli residues fermented with probiotics on the growth performance and health status of broilers challenged with Clostridium perfringens. Braz J Poult Sci. 2018a Oct-Dec;20(4):625-32. https://doi.org/10.1590/1806-9061-2018-0741
 
Liu N, Ding K, Wang J, Deng Q, Gu K, Wang J. Effects of lactic acid bacteria and smectite after aflatoxin B1 challenge on the growth performance, nutrient digestibility and blood parameters of broilers. J Anim Physiol Anim Nutr (Berl). 2018b Aug;102(4):953-61. https://doi.org/10.1111/jpn.12901
 
Liu N, Wang JQ, Liu ZY, Wang YC, Wang JP. Comparison of probiotics and clay detoxifier on the growth performance and enterotoxic markers of broilers fed diets contaminated with aflatoxin B1. J Appl Poult Res. 2018c Sep;27(3):341-8. https://doi.org/10.3382/japr/pfy003
 
Liu N, Lin L, Wang JQ, Zhang FK, Wang JP. Tetramethylpyrazine supplementation reduced Salmonella Typhimurium load and inflammatory response in broilers. Poult Sci. 2019 Aug 1;98(8):3158-64. https://doi.org/10.3382/ps/pez128
 
Lowe TM, Eddy SR. tRNAscan-SE: A program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res. 1997 Mar 1;25(5):955-64. https://doi.org/10.1093/nar/25.5.955
 
Morris R, Kershaw NJ, Babon JJ. The molecular details of cytokine signaling via the JAK/STAT pathway. Protein Sci. 2018 Dec;27(12):1984-2009. https://doi.org/10.1002/pro.3519
 
Saadat YR, Khosroushahi AY, Movassaghpour AA, Talebi M, Gargari BP. Modulatory role of exopolysaccharides of Kluyveromyces marxianus and Pichia kudriavzevii as probiotic yeasts from dairy products in human colon cancer cells. J Funct Foods. 2020 Jan;64:103675. https://doi.org/10.1016/j.jff.2019.103675
 
Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc. 2008;3(6):1101-8. https://doi.org/10.1038/nprot.2008.73
 
Schwartz DM, Kanno Y, Villarino A, Ward M, Gadina M, O’Shea JJ. JAK inhibition as a therapeutic strategy for immune and inflammatory diseases. Nat Rev Drug Discov. 2017 Dec;16(12):843-62. Erratum in: Nat Rev Drug Discov. 2017 Dec 28;17(1):78. https://doi.org/10.1038/nrd.2017.201
 
Shi H, Deng X, Deng Q, Liu Z, Liu Z. Probiotic Lactobacilli attenuated Salmonella Typhimurium infection via Jak/STAT signaling in broilers. Braz J Poult Sci. 2021;23(1):1-7. https://doi.org/10.1590/1806-9061-2020-1328
 
Sisak F, Havlickova H, Hradecka H, Rychlik I, Kolackova I, Karpiskova R. Antibiotic resistance of Salmonella spp. isolates from pigs in the Czech Republic. Vet Med-Czech. 2006;51(5):303-10. https://doi.org/10.17221/5550-VETMED
 
Stastnik O, Mrkvicova E, Pavlata L, Anzenbacherova E, Pro-kop J, Roztocilova A, Umlaskova B, Novotny J, Met-narova E, Vyhnanek T, Trojan V. Purple wheat as a source of anthocyanins and its effect on the metabolism of rabbits. Vet Med-Czech. 2019;64(12):539-46. https://doi.org/10.17221/40/2019-VETMED
 
Truong AD, Rengaraj D, Hong Y, Hoang CT, Hong YH, Lillehoj HS. Differentially expressed JAK-STAT signaling pathway genes and target microRNAs in the spleen of necrotic enteritis-afflicted chicken lines. Res Vet Sci. 2017 Dec;115:235-43. https://doi.org/10.1016/j.rvsc.2017.05.018
 
Upadhaya SD, Hossiendoust A, Kim IH. Probiotics in Salmonella-challenged Hy-Line brown layers. Poult Sci. 2016 Aug 1;95(8):1894-7. https://doi.org/10.3382/ps/pew106
 
Wang L, Li L, Lv Y, Chen Q, Feng J, Zhao X. Lactobacillus plantarum restores intestinal permeability disrupted by Salmonella infection in newly-hatched chicks. Sci Rep. 2018 Feb 2;8(1):2229. https://doi.org/10.1038/s41598-018-20752-z
 
Wang J, Lin L, Jiang Q, Huang W, Liu N. Effect of supplemental lactic acid bacteria on growth performance, glutathione turnover and aflatoxin B1 removal in lambs. Czech J Anim Sci. 2019a;64(6):272-8. https://doi.org/10.17221/5/2019-CJAS
 
Wang J, Lin L, Li B, Zhang F, Liu N. Dietary Artemisia vulgaris meal improved growth performance, gut microbes, and immunity of growing Rex rabbits. Czech J Anim Sci. 2019b;64(4):174-9. https://doi.org/10.17221/162/2018-CJAS
 
Wang K, Ran L, Yan T, Niu Z, Kan Z, Zhang Y, Yang Y, Xie L, Huang S, Yu Q, Wu D. Anti-TGEV miller strain infection effect of Lactobacillus plantarum supernatant based on the JAK-STAT1 signaling pathway. Front Microbiol. 2019c Nov 6;10:2540. https://doi.org/10.3389/fmicb.2019.02540
 
Wongsen S, Werawatganon D, Tumwasorn S. Lactobacillus plantarum B7 attenuates Salmonella Typhimurium infection in mice: Preclinical study in vitro and in vivo. Asian Biomed. 2019;12(5):211-8. https://doi.org/10.1515/abm-2019-0022
 
Yin MC, Chang CH, Su CH, Yu B, Hsu YM. Pteris multifida, Cortex phellodendri, and probiotics attenuated inflammatory status and immunity in mice with a Salmonella enterica serovar Typhimurium infection. Biosci Biotechnol Biochem. 2018 May;82(5):836-47. https://doi.org/10.1080/09168451.2018.1447356
 
Zhao H, Zhang F, Chai J, Wang J. Effect of lactic acid bacteria on Listeria monocytogenes infection and innate immunity in rabbits. Czech J Anim Sci. 2020a;65(1):23-30. https://doi.org/10.17221/247/2019-CJAS
 
Zhao H, Zhang F, Chai J, Wang J. Lactobacillus acidophilus reduces Listeria monocytogenes infection by inhibiting mitogen-activated protein kinase genes in growing rabbits. R Bras Zootec. 2020b;49:e20200054. https://doi.org/10.37496/rbz4920200054
 
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti