The effect of Origanum syriacum L. extract and carvacrol on the in vitro digestion, estimated digestion values, ammonia and organic acid concentrations in the fermentation fluid of lucerne herbage

https://doi.org/10.17221/35/2021-VETMEDCitation:

Onel SE, Aksu T, Kara K, Aksu DS (2022): The effect of Origanum syriacum L. extract and carvacrol on the in vitro digestion, estimated digestion values, ammonia and organic acid concentrations in the fermentation fluid of lucerne herbage. Vet Med-Czech 67, 309–315.

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The effects induced by medicinal aromatic plants in biological systems vary with the type and amount of bioactive substances these plants contain. Whether the purified form of the main chemical components of these plants, such as carvacrol and thymol, or plant volatile oils containing tens of bioactive compounds are more effective remains a question of debate. This study was aimed at providing a comparative assessment of the effects of Origanum syriacum L. (wild mountain thyme) volatile oil (OSVO) and one of its main components, carvacrol (CRV), on the in vitro ruminal degradability of lucerne herbage and methane production during the degradation of lucerne. For this purpose, wild thyme was harvested at the beginning of the flowering period, and the OSVO was extracted from the plant by steam distillation. Gas production assays were performed in five groups of ruminal fluid samples, one of which was maintained for control purposes, and the other four 40/60/80 mg/l of OSVO and 60 mg/l of CRV were added. Compared to the control group, in the samples with the added CRV and OSVO, the amounts of in vitro total gas and methane production were observed to have been affected, but no decrease was detected in the ruminal protozoa counts. The level of ammonia nitrogen was lowest in the groups, in which CRV and 40 mg/l of OSVO (P < 0.01) were added. The ruminal protozoa counts were not affected by the addition of CRV and OSVO. While the total volatile fatty acid (TVFA) and propionic acid (PA) concentrations in the in vitro fermentation fluid of lucerne herbage were low in all the groups, butyric acid was detected at a level of 40 mg/l in the group where CRV was added. The OSVO was ascertained to have induced dose-dependent alterations in the investigated in vitro digestion parameters. In result, CRV (60 mg/l) and OSVO (40 mg/l) were determined to have shown a relatively positive effect on the in vitro ruminal gas production. The anti-methanogenic effect of the plant extracts was due to the decreased digestibility of the lucerne herbage. This can have a positive impact on the environment, but the same cannot be said for the animal nutrient use and animal performance.

References:
AOAC – Association of Official Analytical Chemists. Official methods of analysis of AOAC international. Washington, DC: Association of Official Analytical Chemists; 1995.
 
Aksu T, Saripinar AD, Kaya DA, Duran N, Onel SE, Canogullari S. The response of Japanese quails to dietary Thymbra spicata L. essential oil. Rom Biotech Lett. 2018;23(5):13909-17.
 
Baytok E, Kara K, Aksu T, Guclu BK, Ozkaya S, Denek N, Akcay A. The effect of Mediterranean thyme (Thymbra spicata L. var. spicata) essential oil on fattening performance and ruminal parameters in lamb. J Anim Feed Sci. 2017;26(4):319-25. https://doi.org/10.22358/jafs/80089/2017
 
Blummel M, Makkar HPS, Becker K. In vitro gas production: A technique revisited. J Anim Physiol Anim Nutr. 1997;77(1):24-34. https://doi.org/10.1111/j.1439-0396.1997.tb00734.x
 
Boussaada A, Arhab R, Calabro S, Grazioli R, Ferrara M, Musco N, Cutrignelli MI. Effect of Eucalyptus globulus leaves extracts on in vitro rumen fermentation, methanogenesis, degradability and protozoa population. Ann Anim Sci. 2018;18(3):753-67. https://doi.org/10.2478/aoas-2018-0006
 
Boyne AW, Eadie JM, Raitt K. The development and testing of a method of counting rumen ciliate protozoa. J Gen Microbiol. 1957 Oct;17(2):414-23. https://doi.org/10.1099/00221287-17-2-414
 
Castaneda-Correa A, Corral-Luna A, Hume ME, Anderson RC, Ruiz-Barrera O, Castillo-Castillo Y, Rodriguez-Almeida F, Salinas-Chavira J, Arzola-Alvarez C. Effects of thymol and carvacrol, alone or in combination, on fermentation and microbial diversity during in vitro culture of bovine rumen microbes. J Environ Sci Health B. 2019;54(3):170-5. https://doi.org/10.1080/03601234.2018.1536580
 
Cobellis G, Trabalza-Marinucci M, Marcotullio MC, Yu Z. Evaluation of different essential oils in modulating methane and ammonia production, rumen fermentation, and rumen bacteria in vitro. Anim Feed Sci Technol. 2016;215:25-36. https://doi.org/10.1016/j.anifeedsci.2016.02.008
 
Cook CM, Maloupa E, Kokkini S, Lanaras T. Differences between the inflorescence, leaf and stem essential oils of wild Mentha pulegium plants from Zakynthos, Greece. J Essent Oil Res. 2007;19(3):239-43. https://doi.org/10.1080/10412905.2007.9699269
 
Gerber PJ, Hristov AN, Henderson B, Makkar H, Oh J, Lee C, Meinen R, Montes F, Ott T, Firkins J, Rotz A, Dell C, Adesogan AT, Yang WZ, Tricarico JM, Kebreab E, Waghorn G, Dijkstra J, Oosting S. Technical options for the mitigation of direct methane and nitrous oxide emissions from livestock: A review. Animal. 2013 Jun;7(Suppl_2):220-34. https://doi.org/10.1017/S1751731113000876
 
Hristov AN, Oh J, Firkins JL, Dijkstra J, Kebreab E, Waghorn G, Makkar HP, Adesogan AT, Yang W, Lee C, Gerber PJ, Henderson B, Tricarico JM. Special topics – Mitigation of methane and nitrous oxide emissions from animal oper-ations: I. A review of enteric methane mitigation options. J Anim Sci. 2013 Nov;91(11):5045-69. https://doi.org/10.2527/jas.2013-6583
 
IPCC. Climate change: Impacts, adaptation, and vulnerability. Part B: Regional aspects. Contribution of working group II to the fifth assessment report of the inter-governmental panel on climate change. New York, NY, USA: Cambridge University Press; 2014. 688 p.
 
Jafari S, Ebrahimi M, Goh YM, Rajion MA, Jahromi MF, Al-Jumaili WS. Manipulation of rumen fermentation and methane gas production by plant secondary metabolites (saponin, tannin and essential oil) – A review of ten-year studies. Ann Anim Sci. 2019;19(1):3-29. https://doi.org/10.2478/aoas-2018-0037
 
Jimenez-Peralta FS, Salem AZM, Mejia-Hernandez P, Gonzalez-Ronquillo M, Albarran-Portillo B, Rojo-Rubio R, Tinoco-Jaramillo JL. Influence of individual and mixed extracts of two tree species on in vitro gas production kinetics of a high concentrate diet fed to growing lambs. Livest Sci. 2011;136(2-3):192-200. https://doi.org/10.1016/j.livsci.2010.09.008
 
Karik U, Cicek F, Tutar M, Ayas F. Essential oil compounds of Turkey laurel (Laurus nobilis L.) populations. Anadolu J AARI. 2015;25(1):1-16.
 
Mandal GP, Roy A, Patra AK. Effects of plant extracts rich in tannins, saponins and essential oils on rumen fermentation and conjugated linoleic acid conentrations in vitro. Indian J Anim Hlth. 2016;55(1):49-60.
 
Menke KH, Raab L, Salewsk A, Steingass H, Fritz D, Schneide W. The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro. J Agric Sci Comb. 1979;93(1):217-22. https://doi.org/10.1017/S0021859600086305
 
Onel SE, Aksu T. Can thyme (Thymbra spicata L. var. spicata) volatile oil alleviate the detrimental effects of high stocking densities in Japanese quail? Turk J Vet Anim Sci. 2020;44(5):1024-31.
 
Onel SE, Aksu T, Kara K, Aksu DS. Effects of essential oils derived from different thyme species on in vitro nutrient digestibility, rumen protozoa number and methane production of alfalfa hay. 2nd International Animal Nutrition Congress Antalya, Turkey, November 1-4; 2018. p. 108-12.
 
Onel SE, Aksu T, Kara K, Aksu DS. The effects of Laurel volatile oil (Laurusnobilis L.) on in vitro ruminal gas production of methane emission, organic acids and protozoa counts alfalfa herbage. Erciyes Üniv Vet Fak Derg. 2020;17(3):283-9.
 
Patra AK, Yu Z. Effects of essential oils on methane production and fermentation by, and abundance and diversity of, rumen microbial populations. Appl Environ Microbiol. 2012 Jun;78(12):4271-80. https://doi.org/10.1128/AEM.00309-12
 
Patra AK, Yu Z. Effects of vanillin, quillaja saponin, and essential oils on in vitro fermentation and protein-degrading microorganisms of the rumen. Appl Microbiol Biotechnol. 2014 Jan;98(2):897-905. https://doi.org/10.1007/s00253-013-4930-x
 
Sallam SM, Abdelgaleil SA, Bueno IC, Nasser ME, Araujo RC, Abdalla AL. Effect of some essential oils on in vitro methane emission. Arch Anim Nutr. 2011 Jun;65(3):203-14. https://doi.org/10.1080/1745039X.2011.568275
 
Soltan Y, Morsy AS, Araujo RC, Elziat HM, Sallam SMA, Louvandini H, Abdalla AL. Carvacrol and eugenol as modifiers of rumen microbial fermentation, and methane production in vitro. Proceedings of 4th Animal Wealth Research Conference in the middle East and North Africa; Egypt, Oct 3-5; 2011. p. 354-64.
 
Van Soest PJ, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci. 1991 Oct;74(10):3583-97. https://doi.org/10.3168/jds.S0022-0302(91)78551-2
 
Wanapat M, Cherdthong A, Phesatcha K, Kang S. Dietary sources and their effects on animal production and environmental sustainability. Anim Nutr. 2015 Sep;1(3):96-103. https://doi.org/10.1016/j.aninu.2015.07.004
 
Zhou R, Wu J, Lang X, Liu L, Casper DP, Wang C, Zhang L, Wei S. Effects of oregano essential oil on in vitro ruminal fermentation, methane production, and ruminal microbial community. J Dairy Sci. 2020 Mar;103(3):2303-14. https://doi.org/10.3168/jds.2019-16611
 
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