Effect of intra-articular administration of autologous PRP and activated PRP on inflammatory mediators in dogs with osteoarthritis

https://doi.org/10.17221/36/2019-VETMEDCitation:Parlak K., Arican M. (2020): Effect of intra-articular administration of autologous PRP and activated PRP on inflammatory mediators in dogs with osteoarthritis. Veterinarni Medicina, 65: 62-70.
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The aim of this study was to investigate the effects of the intra-articular use of platelet rich plasma (PRP) and bio-physically activated PRP (BPRP) on the inflammatory mediators for the treatment of osteoarthritis in dogs. The animals included in this study were 36 mix breed dogs diagnosed with osteoarthritis in the stifle as a result of the clinical and radiological examinations. The dogs were randomly divided into three groups: PRP (platelet-rich plasma), BPRP (biophysically activated platelet-rich plasma) and control (given 0.9% isotonic saline). These three main groups were each further divided into two groups as single and double according to the number of intraarticular administrations. Joint fluid analyses, a clinical examination (Hudson Visual Analog Scale and Canine Brief Pain Inventory Tests) radiographic and ultrasonographic examinations were performed on days 1, 15, 30, 60, and 90 for each group. Genesis System 2 branded and BPRP preparation kits were used in this study. An ELISA method was used to measure the cytokines in charge of the inflammatory mediation (IL-1β, IL-6, IL-10, TNF-α) in the synovial fluid samples. The records obtained from the walking and pain rating tests were subjected to a statistical analysis program and a Mann-Whitney U test was performed. The results of the ELISA were evaluated by a Tukey test. There was a significant difference between the single and double groups of the PRP administration on days 60 and 90 (P < 0.05) in the walking and pain scores. The double groups of the PRP had better results than the single groups. There was a significant difference between the single groups of the PRP and BPRP for the IL-10 on the 30th day (P < 0.05). In the single application groups, the BPRP was better than the PRP on day 30 in the IL-10 measurements. In the comparison of the single and double administration groups, there was significant difference between the single and double groups of the BPRP on day 90 (P < 0.05). The double groups of the BPRP had better results than the single groups. In addition, the biophysically activated PRP was found to be superior to the PRP for the IL-10 content. In conclusion, the efficacy of the PRP and BPRP was related to the degree of the osteoarthritis (OA). Especially the success rate in the acute OA patients was higher due to the anti-inflammatory activity of the BPRP. Moreover, the double administration groups gave more positive results than the single administration groups.

References:
Anitua E, Sanchez M, Fuente M, Azofra J, Zalduendo M, Aguirre JJ, Andia I. Relationship between investigative biomarkers and radiographic grading in patients with knee osteoarthritis. Int J Rheumatol. 2009 Apr;2009(5):747432. https://doi.org/10.1155/2009/747432
 
Aragon CL, Hofmeister EH, Budsberg SC. Systematic review of clinical trials of treatments for osteoarthritis in dogs. J Am Vet Med Assoc. 2007 Feb;230(4):514-21. https://doi.org/10.2460/javma.230.4.514
 
Arican M. Köpeklerin Dejeneratif Eklem Hastalıklarında (Osteoartritis) Güncel Teşhis ve Tedavi Yöntemleri [Current diagnosis and treatment methods in degenerative joint diseases (osteoarthritis) in dogs]. Turkiye Klinikleri J Vet Sci. 2014 Jul;5(2):1-9. Turkish.
 
Arican M, Simsek A, Parlak K, Atli K, Sen G. Investigation of the effect of intra-articular autologous platelet-rich plasma (PRP) on inflammatory mediators and metalloproteinase (MMP-2 and MMP-9) enzymes in osteoarthritis dogs. Tubitak Project. 213O175. 2015. p. 18-60.
 
Brenn D, Richter F, Schaible H. Sensitization of unmyelinated sensory fibers of the joint nerve to mechanical stimuli by interleukin-6 in the rat: an inflammatory mechanism of joint pain. Arthritis Rheum. 2007 Jan;56(1):351-9. https://doi.org/10.1002/art.22282
 
Brown D, Boston RC, Coyne JC, Farrar JT. Ability of the canine brief pain inventory to detect response to treatment in dogs with osteoarthritis. J Am Vet Med Assoc. 2008 Oct;233(8):1278-83. https://doi.org/10.2460/javma.233.8.1278
 
Fahie MA, Ortolano GA, Gurecio V, Schaffer JA, Johnston G, Au J, Hettlich BA, Philips T, Allen MJ, Bertone AL. A randomized controlled trial of the efficacy of autologous plasma rich platelet therapy for the treatment of osteoarthritis in dogs. J Am Vet Med Assoc. 2013 Nov;243(9):1291-7.  https://doi.org/10.2460/javma.243.9.1291
 
Fernandes JC, Martel-Pelletier J, Pelletier JP. The role of cytokines in osteoarthritis pathophysiology. Biorheology. 2002 Feb;39(1-2):237-46.
 
Grassi W, Cervini C. Ultrasonography in rheumatology: an evolving technique. Ann Rheum Dis. 1998 May;57(5):268-71.  https://doi.org/10.1136/ard.57.5.268
 
Hay CW, Chu Q, Budsberg SC, Clayton MK, Johnson KA. Synovial fluid interleukin 6, tumor necrosis factor and nitric oxide values in dogs with osteoarthritis secondary to cranial cruciate ligament rupture. Am J Vet Res. 1997 Sep;58(9):1027-32.
 
Hudson JT, Slater MR, Taylor L, Scott HM, Kerwin SC. Assessing repeatability and validity of a visual analogue scale questionnaire for use in assessing pain and lameness in dogs. Am J Vet Res. 2004 Dec;65(12):1634-43. https://doi.org/10.2460/ajvr.2004.65.1634
 
Innes JF, Costello M, Barr FJ, Rudorf H, Barr AR. Radiographic progression of osteoarthritis of the canine stifle joint: A prospective study. Vet Radiol Ultrasound. 2004 Mar-Apr;45(2):143-8. https://doi.org/10.1111/j.1740-8261.2004.04024.x
 
Jansen NW, Roosendaal G, Hooiveld MJ, Bijlsma JW, van Roon JA, Theobald M, Lafeber FP. Interleukin-10 protects against blood-induced joint damage. Br J Haematol. 2008 Sep;142(6):953-61. https://doi.org/10.1111/j.1365-2141.2008.07278.x
 
Kellegren JH, Lawrence JS. Radiological assessment of osteo-arthrosis. Ann Rheum Dis. 1957 Dec;16(4):494-502. https://doi.org/10.1136/ard.16.4.494
 
Knop E, Paula LE, Fuller R. Platelet-rich plasma for osteoarthritis treatment. Rev Bras Reumatol Engl Ed. 2016 Mar-Apr;56(2):152-64. https://doi.org/10.1016/j.rbre.2015.07.002
 
Ley C, Ekman S, Elmen A, Nilsson G, Eloranta ML. Interleukin-6 and tumour necrosis factor in synovial fluid from horses with carpal joint pathology. J Vet Med A Physiol Pathol Clin Med. 2007 Sep;54(7):346-51.  https://doi.org/10.1111/j.1439-0442.2007.00956.x
 
Lohmander LS. Articular cartilage and osteoarthrosis. The role of molecular markers to monitor breakdown, repair and disease. J Anat. 1994 Jun;184(Pt 3):477-92.
 
Maccoux LJ, Salway F, Day PJR, Clements DN. Expression profiling of select cytokines in canine osteoarthritis tissues. Vet Immunol Immunopathol. 2007 Jul;118;(1-2):59-67. https://doi.org/10.1016/j.vetimm.2007.04.006
 
Muller S, Kramer M. Die Eignung der Sonographie für die Diagnostik von Meniskusläsionen beim Hund [The use of ultrasonography in the diagnostic of meniscus lesions in the dog]. Tierärztliche Praxis. 2003 Jan;31(1):10-5. German. https://doi.org/10.1055/s-0037-1622107
 
Muzzi L, Rezende C, Muzzi R. Fisioterapia após substituição artroscópica do ligamento cruzado cranial em cães: I - avaliação clínica, radiográfica e ultrassonográfica [Physiotherapy after arthroscopic repair of the cranial cruciate ligament in dogs. I - clinical, radiographic, ultrasonographic evaluation]. Arq Bras Med Vet Zootec. 2009 Aug;61(4):805-14. Portuguese. https://doi.org/10.1590/S0102-09352009000400007
 
Pietrzak WS, Eppley BL. Platelet rich plasma: Biology and new technology. J Craniofac Surg. 2005 Nov;16(6):1043-54. https://doi.org/10.1097/01.scs.0000186454.07097.bf
 
Pochini AC, Antonioli E, Bucci DZ, Sardinha LR, Andreoli CV, Ejnisman B, Goldberg AC, Cohen M. Analysis of cytokine profile and growth factors in platelet-rich plasma obtained by open systems and commercial columns. Einstein (Sao Paulo). 2016 Jul-Sep;14(3):391-7.  https://doi.org/10.1590/S1679-45082016AO3548
 
Sanderson O, Beata C, Flipo RM, Genevois JP, Marcias C, Tacke S, Vezzoni A, Innes JF. Systematic review of the management of canine osteoarthritis. Vet Rec. 2009 Apr;164(14):418-24. https://doi.org/10.1136/vr.164.14.418
 
Shakibaei M, Schulze-Tenzil G, John T, Mobasheri A. Curcumin protects human chondrocytes from IL-1β-induced inhibition of collagen type II and β1-integrin expression and activation of caspase-3: An immunomorphological study. Ann Anat. 2005 Nov;187(5-6):487-97. https://doi.org/10.1016/j.aanat.2005.06.007
 
Stief M, Gottschalk J, Ionita JC, Einspanier A, Oechtering G, Bottcher P. Concentration of platelets and growth factors in canine autologous conditioned plasma. Vet Comp Orthop Traumatol. 2011 Jan;24(2):122-5. https://doi.org/10.3415/VCOT-10-04-0064
 
Sundman EA, Cole BJ, Fortier LA. Growth factor and catabolic cytokine concentrations are influenced by the cellular composition of platelet-rich plasma. Am J Sports Med. 2011 Oct;39(10):2135-40.  https://doi.org/10.1177/0363546511417792
 
Tamura T, Shirai T, Kosaka N, Ohmori K, Takafumi N. Pharmacological studies of diacerein in animal models of inflammation, arthritis and bone resorption. Eur J Pharmacol. 2002 Jul;448(1):81-7. https://doi.org/10.1016/S0014-2999(02)01898-8
 
Venn G, Nietfeld JJ, Duits AJ, Brennan FM, Arner E, Covington M, Billingham ME, Hardingham TE. Elevated synovial fluid levels of interleukin-6 and tumor necrosis factor associated with early experimental canine osteoarthritis. Arthritis Rheum. 1993 Jun;36(6):819-26. https://doi.org/10.1002/art.1780360613
 
Yilmaz B, Kesikburun S. Plateletten zengin plazma uygulamalari [Platelet rich plasma applications]. Turk Fiz Tip Rehab Derg. 2013 Dec;59(4):338-44. Turkish. https://doi.org/10.4274/tftr.26594
 
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