Morphological and morphometric adaptations of testes in broilers induced by glucocorticoid

https://doi.org/10.17221/38/2021-VETMEDCitation:

Islam R, Sultana N, Ayman U, Akter A, Afrose M, Haque Z (2021): Morphological and morphometric adaptations of testes in broilers induced by glucocorticoid. Vet Med-Czech 66, 520–529.

download PDF

Glucocorticoids (GCs) cause excess fat accumulation, which leads to fertility dysfunction in broilers. The study investigated alterations in the morphology and morphometry of the testes of broilers in response to GC and dexamethasone (DEX). Male day-old chicks were randomly divided into a control group and three experimental groups (E1, E2, and E3). The control group was fed a commercial broiler ration. The experimental groups were fed a commercial broiler ration containing GC (i.e. DEX 3, 5, and 7 mg/kg, respectively). The testes were collected and stained with haematoxylin and eosin to count the number of testicular seminiferous tubules. An increase in the seminiferous tubule count was initially seen, which declined as both the age of the broilers and the dose of DEX increased. Morphometric measurements, i.e., the testicular capsule thickness, seminiferous tubule diameter, and seminiferous epithelium height, were performed. The initial thickening of the testicular capsule was evident. There was a depletion of the interstitial (Leydig) cell population in the experimental groups with the age and increased with the dose advancement. The diameter of the seminiferous tubules and testicular capsule thickness remained upregulated in the treatment groups with the increased dose of DEX. The initial height of the seminiferous epithelium increased in the experimental groups of broilers. The study suggests that DEX greatly alters the morphological architecture of broiler testes; as a result, it could be said that DEX has the effect on the infertility of the broiler by affecting the morphology as well as the functionality of the testes.

References:
Abo-Youssef AA, Mohammed WY, Youssef YM. Histological study of the effect of administration of exogenous glucocorticoids on the testis of albino rats. Egypt Acad J Biol Sci. 2018 Mar;10(1):1-12. https://doi.org/10.21608/eajbsd.2018.14039
 
Afrose M, Sultana N, Islam MR. Physiological responses of corticosteroid, dexamethasone in broiler chicken. Int J Sci Res. 2018 Dec;7(12):1459-63.
 
Begum IA, Alam MJ, Buysse J, Frija A, Huylenbroeck GV. Contract farmer and poultry farm efficiency in Bangladesh: A data envelopment analysis. Appl Econ. 2011 Aug; 44(28):3737-47. https://doi.org/10.1080/00036846.2011.581216
 
Badawy GM. Testicular toxicity induced by exogenous betamethasone and the ameliorative role of curcumin in adult and fetal stages of albino rats. Eur J Pharm Med Res. 2018 Dec;5(12):599-615.
 
Bonetti A, Tirelli F, Catapano A, Dazzi D, Cas AD, Solito F, Ceda G, Reverberi C, Monica C, Pipitone S, Elia G, Spattini M, Magnati G. Side effects of anabolic androgenic steroids abuse. Int J Sports Med. 2008 Aug;29(8):679-87. https://doi.org/10.1055/s-2007-965808
 
Chowdhury SD. Commercial poultry farming in Bangladesh: The rolling tears of farmers and its consequences. In: Proceedings of the 7th International Poultry Show and Seminar; 2011 Mar 25-7; Dhaka, Bangladesh: World's Poultry Science Association; 2011. p. 1-12.
 
Crisostomo L. Molecular mechanisms and signaling pathways involved in the nutritional support of spermatogenesis by Sertoli cells. In: Alves M, Oliveira P, editors. Sertoli cells. Book series: Methods in molecular biology, 1748. New York: Humana Press; 2018. p. 129-55.
 
Fragoso SJ, Diaz MP, Moreno JCA, Infesta PC, Bertos AR, Barger K. Relationships between fertility and some parameters in male broiler breeders (Body and testicular weight, histology and immunohistochemistry of testes, spermatogenesis and hormonal levels). Reprod Domes Anim. 2013 Apr;48(2):345-52. https://doi.org/10.1111/j.1439-0531.2012.02161.x
 
Franca L, Russell L. The testis of domestic animals. In: Martinez-Garcia F, Regadera J, editors. Male reproduction. A multidisciplinary overview. Madrid: Churchill Living Stone; 1998. p. 197-219.
 
Hameed U, Iqbal S, Rehman F, Hassan A, Memon AM, Mustafa S. Effect of exogenous and endogenous glucocorticoids on the spermatogenesis of albino rats: A comparative study. Annals ASH KMDC. 2020 Jan;25(3):151.
 
Hardy MP, Gao HB, Dong Q, Ge R, Wang Q, Chai WR, Feng X, Sottas C. Stress hormone and male reproductive function. Cell Tissue Res. 2005 Oct;322(1):147-53. https://doi.org/10.1007/s00441-005-0006-2
 
Ing NH, Forrest DW, Riggs PK, Loux S, Love CC, Brinsko SP, Varner DD, Welsh TH Jr. Dexamethasone acutely down-regulates genes involved in steroidogenesis in stallion testes. J Steroid Biochem Mol Biol. 2014 Sep;143:451-9. https://doi.org/10.1016/j.jsbmb.2014.07.003
 
Jiang KJ, Jiao HC, Song ZG, Yuan L, Zhao JP, Lin H. Corticosterone administration and dietary glucose supplementation enhance fat accumulation in broiler chickens. Br Poult Sci. 2008 Sep;49(5):625-31. https://doi.org/10.1080/00071660802337241
 
Khayat NMH, Safavi SE, Erik AH. The effect of ketoconazol chronic administration on histomorphometric changes of testicular tissue in mice. J Vet Med. 2009;3(8):45-53.
 
Kheirabad MK, Jahromi BN, Rahmanifar F, Tamadon A, Owjfard M. Effect of chronic stress on stereological properties of seminiferous tubules in adult male rats. Int J Fertil Steril. 2015;9(1):45.
 
Kheirabad MK, Khodabandeh Z, Rahmanifar F, Tamadon A, Jahromi BN, Owjfard M, Hosseinabadi OK. Testicular germ cells apoptosis following exposure to chronic stress in rats. Asian Pac J Reprod. 2016 Sep;5(5):371-5. https://doi.org/10.1016/j.apjr.2016.07.005
 
Li Y, Sun Y, Chen J. [Research progresses on effects of fat deposition on reproduction performance of poultry]. China Poult. 2016;38(13):36-41. Chinese.
 
Lv ZP, Peng YZ, Zhang BB, Fan H, Liu D, Guo YM. Glucose and lipid metabolism disorders in the chickens with dexamethasone-induced oxidative stress. J Anim Physiol Anim Nutr (Berl). 2018 Apr;102(2):e706-17. https://doi.org/10.1111/jpn.12823
 
Mahmoud H, Mahmoud O, Layasadat K, Naeim A. Dexamethasone effects on Bax expression in the mouse testicular germ cells. Folia Histochem Cytobiol. 2009;47(2):237-41. https://doi.org/10.2478/v10042-009-0041-z
 
Mao BP, Li L, Yan M, Lian Q, Ge R, Cheng CY. Environmental toxicants and cell polarity in the testis. Reprod Toxicol. 2018 Oct;81:253-8. https://doi.org/10.1016/j.reprotox.2018.08.015
 
Mostafa MME, El-Faham AI, Ahmed AHH. Effect of different sources of growth promoter supplementation in diet on performance, carcass traits and blood constituents of broiler chicks. Asian J Anim Vet Adv. 2016 Jan;11(2):106-13. https://doi.org/10.3923/ajava.2016.106.113
 
Neilsen WE, Kaye AD. Steroids: Pharmacology, complications, and practice delivery issues. Ochsner J. 2014 Summer;14(2):203-7.
 
Oray M, Abu Samra K, Ebrahimiadib N, Meese H, Foster CS. Long-term side effects of glucocorticoids. Expert Opin Drug Saf. 2016;15(4):457-65. https://doi.org/10.1517/14740338.2016.1140743
 
Rai J, Pandey SN, Srivastava RK. Effect of immobilization stress on spermatogenesis of albino rats. J Anat Soc India. 2003;52(1):1-12.
 
Rastrelli G, Corona G, Maggi M. Testosterone and sexual function in men. Maturitas. 2018 Jun;112:46-52. https://doi.org/10.1016/j.maturitas.2018.04.004
 
Sultana N, Amin T, Afrose M, Rony SA, Rafiq K. Effects of dexamethasone on the morphometry and biometry of immune organs of broiler chicken. Int J Morph. 2020a Aug;38(4):1032-8. https://doi.org/10.4067/S0717-95022020000401032
 
Sultana N, Afrose M, Rafiq K. Effects of steroid growth promoter on morphological and biochemical adaptations in liver of broiler. Vet World. 2020b Nov;13(11):2330-7. https://doi.org/10.14202/vetworld.2020.2330-2337
 
Welter H, Herrmann C, Dellweg N, Missel A, Thanisch C, Urbanski HF, Kohn FM, Schwarzer JU, Taubenberger AM, Mayerhofer A. The glucocorticoid receptor NR3C1 in testicular peritubular cells is developmentally regulated and linked to the smooth muscle-like cellular phenotype. J Clin Med. 2020 Mar 31;9(4):961. https://doi.org/10.3390/jcm9040961
 
Yazawa H, Sasagawa I, Nakada T. Apoptosis of testicular germ cells induced by exogenous glucocorticoid in rats. Human Reprod. 2000 Sep;15(9):1917-20. https://doi.org/10.1093/humrep/15.9.1917
 
download PDF

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti