Stability of haematological parameters in stored blood samples of rainbow trout Oncorhynchus mykiss (Walbaum, 1792) F., Ferrantelli V., Saoca C., Giangrosso G., Piccione G. (2017): Stability of haematological parameters in stored blood samples of rainbow trout Oncorhynchus mykiss (Walbaum, 1792). Veterinarni Medicina, 62: 401-405.
download PDF
The aim of this study was to investigate the influence of storage time at +4 °C on haematological indicators in rainbow trout Oncorhynchus mykiss (Walbaum, 1792). For this purpose, 60 Italian trout were evaluated and red blood cells, white blood cells, thrombocyte count, haematocrit, haemoglobin concentration, mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration were determined. After blood collection (T0), all samples were immediately analysed using an automatic method to assess the haematological parameters and then divided into two different aliquots and stored at +4 °C. The first aliquot was refrigerated for 168 h (T1), the second one for 336 h (T2). Statistical analysis (one-way repeated-measures ANOVA) showed a significant effect of storage time (P < 0.05) on red blood cells, thrombocyte count, haematocrit, haemoglobin concentration, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration. The obtained data expand our knowledge of haematological evaluation techniques and could contribute to the development of an appropriate method for haematological analysis that is suitable for the evaluation of the health status of wild and farmed fish.
Ballarin Loriano, Dall'Oro Manuela, Bertotto Daniela, Libertini Angelo, Francescon Antonia, Barbaro Alvise (2004): Haematological parameters in Umbrina cirrosa (Teleostei, Sciaenidae): a comparison between diploid and triploid specimens. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 138, 45-51
Gil barcellos Leonardo Jose, Kreutz Luiz Carlos, Rodrigues Laura Beatriz, Fioreze Irineo, Quevedo Rosmari Mezzalira, Cericato Leonardo, Conrad Jaqueline, Soso Auren Benck, Fagundes Michele, De almeida lacerda Luciana, Terra Silvia (2003): Haematological and biochemical characteristics of male jundia (Rhamdia quelen Quoy & Gaimard Pimelodidae): changes after acute stress. Aquaculture Research, 34, 1465-1469
BLEUL U., BLEUL U., SOBIRAJ A., BOSTEDT H. (2002): Effects of Duration of Storage and Storage Temperature on Cell Counts of Bovine Blood Samples as Determined by an Automated Haematology Analyser. Comparative Clinical Pathology, 11, 211-216
Buscaino Giuseppa, Filiciotto Francesco, Buffa Gaspare, Bellante Antonio, Stefano Vincenzo Di, Assenza Anna, Fazio Francesco, Caola Giovanni, Mazzola Salvatore (2010): Impact of an acoustic stimulus on the motility and blood parameters of European sea bass (Dicentrarchus labrax L.) and gilthead sea bream (Sparus aurata L.). Marine Environmental Research, 69, 136-142
Buttarello Mauro (2004): Quality specification in haematology: the automated blood cell count. Clinica Chimica Acta, 346, 45-54
Clark Phillip, Mogg Tony D., Tvedten Harold W., Korcal David (2002): Artifactual Changes in Equine Blood Following Storage, Detected Using the Advia 120 Hematology Analyzer. Veterinary Clinical Pathology, 31, 90-94
Clauss Tonya M., Dove Alistair D.M., Arnold Jill E. (2008): Hematologic Disorders of Fish. Veterinary Clinics of North America: Exotic Animal Practice, 11, 445-462
Davis C, Lebourdais S (2007): Lower cranberry creek: Rainbow trout biology/abundance monitoring (year 1). Prepared for BC Hydro, Revelstoke, Canada. Prepared by Okanagan Nation Alliance, Westbank, Canada. Available at
Faggio C., Casella S., Arfuso F., Marafioti S., Piccione G., Fazio F. (2013): Effect of storage time on haematological parameters in mullet, Mugil cephalus. Cell Biochemistry and Function, 31, 412-416
Fazio Francesco, Filiciotto Francesco, Marafioti Simona, Di Stefano Vincenzo, Assenza Anna, Placenti Francesco, Buscaino Giuseppa, Piccione Giuseppe, Mazzola Salvatore (2012): Automatic analysis to assess haematological parameters in farmed gilthead sea bream ( Sparus aurata Linnaeus, 1758). Marine and Freshwater Behaviour and Physiology, 45, 63-73
Fazio F., Saoca C., Casella S., Fortino G., Piccione G. (): Relationship between blood parameters and biometric indices of Sparus aurata and Dicentrarcus labrax cultured in onshore tanks. Marine and Freshwater Behaviour and Physiology, 48, 289-296
Fazio F, Saoca C, Piccione G, Kesbic OS, Acar U (2016): Comparative study of some hematological and biochemical parameters of Italian and Turkish farmed rainbow trout Oncorhynchus mykiss (Walbaum, 1792). Turkish Journal of Fisheries and Aquatic Sciences 16, 715–721.
Guder W. G. (1999): Preanalytical factors and their influence on analytical quality specifications. Scandinavian Journal of Clinical & Laboratory Investigation, 59, 545-549
Huffman P A., Arkoosh M. R., Casillas E. (1997): Characteristics of Peripheral Blood Cells from Rainbow Trout Evaluated by Particle Counter, Image Analysis, and Hemocytometric Techniques. Journal of Aquatic Animal Health, 9, 239-248<0239:COPBCF>2.3.CO;2
Ihedioha John I., Onwubuche Rebecca C. (2007): Artifactual changes in PCV, hemoglobin concentration, and cell counts in bovine, caprine, and porcine blood stored at room and refrigerator temperatures. Veterinary Clinical Pathology, 36, 60-63
Ihedioha J. I., Idika I. K., Ogamba G. N., Akam C. J. A. (2008): Changes in the haematological values of avian blood samples stored at varying temperatures for a period of up to 72 hours. Comparative Clinical Pathology, 17, 73-79
Micale Valeria, Levanti Maria Beatrice, Germanà Antonino, Guerrera Maria Cristina, Kurokawa Tadahide, Muglia Ugo (2010): Ontogeny and distribution of cholecystokinin-immuno reactive cells in the digestive tract of sharpsnout sea bream, Diplodus puntazzo (Cetti, 1777), during larval development. General and Comparative Endocrinology, 169, 23-27
Nicula M, Bura M, Simiz E, Banatean-Dunea I, Patruica S, Marcu A, Lunca M, Szelei Z (2010): Researches concerning reference values assessment of serum biochemical parameters in some fish species from Acipenseridae, Cyprinidae, Esocidae and Salmonidae family. Journal of Animal Science and Biotechnology 43, 498–505.
Sancho E., Cerón J.J., Ferrando M.D. (2000): Cholinesterase Activity and Hematological Parameters as Biomarkers of Sublethal Molinate Exposure in Anguilla anguilla. Ecotoxicology and Environmental Safety, 46, 81-86
Tavares-Dias M, Moraes FR (eds) (2004): Hematology of Teleost Fishes (in Portuguese). Ribeirao Preto: Villimpress, Sao Paulo. 144 pp.
Tavares-Dias M., Moraes F. R. (2007): Haematological and biochemical reference intervals for farmed channel catfish. Journal of Fish Biology, 71, 383-388
Wells R.M.G., Baldwin J., Seymour R.S., Christian K., Brittain T. (2005): Red blood cell function and haematology in two tropical freshwater fishes from Australia. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 141, 87-93
Wood Brent L., Andrews Judy, Miller Sharon, Sabath Daniel E. (1999): Refrigerated Storage Improves the Stability of the Complete Blood Cell Count and Automated Differential. American Journal of Clinical Pathology, 112, 687-695
download PDF

© 2020 Czech Academy of Agricultural Sciences