P-wave dispersion and renal biomarkers in canine visceral leishmaniasis stage III and IV infected dogs

https://doi.org/10.17221/51/2019-VETMEDCitation:Erdogan S., Erdogan H., Pasa S., Ural K. (2020): P-wave dispersion and renal biomarkers in canine visceral leishmaniasis stage III and IV infected dogs. Veterinarni Medicina, 65: 111-115.
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Canine visceral leishmaniasis is a protozoan disease affecting most vital organs and also causing myocardial and/or renal damage. P-wave dispersion, a newly used non-invasive ECG indicator, is used to follow atrial arrhythmias, atrial fibrillation, valvular disease in both human and veterinary medicine. The purpose of this study involving Canine Visceral Leishmaniasis (CVL)-infected dogs in stage III and IV was to verify whether the P-wave dispersion was related to the renal failure. For this purpose, a total of 17 dogs of different ages, both sexes, comprising eleven animals with CVL (at stage III and IV) diagnosed using a rapid ELISA (enzyme-linked immunosorbent assay) test and serological IFAT (immunofluorescence.antibody titres) and six healthy dogs used as controls were enrolled. Significant differences between the P-wave dispersion (regarding the mean values) of the control, stage III and IV-infected groups (P = 0.003) were detected as follows: 21.8 ± 0.5, 20.6 ± 1.2 and 25.0 ± 0.4 ms, respectively. The P-wave dispersion value was moderately longer in the stage IV-infected group compared with the control one (P = 0.022), however, the aforementioned relationship was determined as significant between the stage IV and III-infected groups (P = 0.003). Regarding all of the biomarkers of the renal function monitored within this study, there were differences between the control and stage IV-infected group for the creatinine (P = 0.002), the control and stage IV-infected group for the urine protein/creatinine ratio (P < 0.001), and the stage III and stage IV-infected groups for the urine protein/creatinine ratio (P < 0.001). In conclusion, an increased P-wave dispersion was not associated with renal failure. It might be related to the limitation of our study including small sample sizes in the groups. Therefore, the cardiac indicators were not analysed. For more accurate results, it is desirable to perform further studies including a larger sample size along with an investigation of the cardiac and renal indicators.

Atar I, Konas ND, Uyar M, Atar A, Bozbas H, Gulmez O, Sezer S, Ozdemir N, Muderrisoglu H, Ozin B. P wave dispersion in chronic renal failure patients with paroxysmal atrial fibrillation. TAPE. 2007;5(1):46-52.
Baneth G, Koutinas AF, Solano–Gallego L, Bourdeau P, Ferrer L. Canine leishmaniosis new concepts and insights on an expanding zoonosis: Part one. Trends Parasitol. 2008 Jul 1;24(7):324-30. https://doi.org/10.1016/j.pt.2008.04.001
Dilaveris PE, Gialafos JE. P-wave dispersion: a novel predictor of paroxysmal atrial fibrillation. Ann Noninvasive Electrocardiol. 2001 Apr;6(2):159-65. https://doi.org/10.1111/j.1542-474X.2001.tb00101.x
Dittrich G, Campos GM, Sousa MG, Oliveira ST. P wave dispersion in obese dogs with and without mitral valve disease. Cienc Rural. 2018 Oct;48(10). https://doi.org/10.1590/0103-8478cr20180047
dos Santos FP, Pascon JPE, Pereira DTP, Anjos BL, Mistieri MLA, Silveira ID, Porciuncula ML. Clinical and histopathological features of myocarditis in dogs with visceral leishmaniasis. Arq Bras Med Vet Zootec. 2015 Dec;67(6):1519-27. https://doi.org/10.1590/1678-4162-7854
Elnojomi N, Musa AM, Younis BM, Elfaki ME, El-Hassan AM, Khalil EAG. Surrogate markers of subtle renal injury in patients with visceral leishmaniasis. Saudi J Kidney Dis Transpl. 2010 Sep 1;21(5):872-5.
Ferroglio E, Centaro E, Mignone W, Trisciuoglio A. Evaluation of an ELISA rapid device for the serological diagnosis of Leishmania infantum infection in dog as compared with immunofluorescence assay and Western blot. Vet Parasitol. 2007 Mar 15;144(1-2):162-6. https://doi.org/10.1016/j.vetpar.2006.09.017
Font A, Durall N, Domingo M, Closa JM, Mascort J, Ferrer L. Cardiac tamponade in a dog with visceral leishmaniasis. J Am Anim Hosp Assoc. 1993;29(2);95-100.
Huang JC, Wei SY, Chen SC, Chang JM, Hung CC, Su HM, Chen HC. P wave dispersion and maximum P wave duration are associated with renal outcomes in chronic kidney disease. PLoS One. 2014 Jul;9(7):e101962. https://doi.org/10.1371/journal.pone.0101962
Khan NA, Ma I, Thompson CR, Humphries K, Salem DN, Sarnak MJ, Levin A. Kidney function and mortality among patients with left ventricular systolic dysfunction. J Am Soc Nephrol. 2006 Jan 1;17(1):244-53. https://doi.org/10.1681/ASN.2005030270
Kollu K, Altintepe L, Duran C, Topal M, Ecirli S. The assessment of P-wave dispersion and myocardial repolarization parameters in patients with chronic kidney disease. Ren Fail. 2018 Nov;40(1):1-7. https://doi.org/10.1080/0886022X.2017.1419962
Lopez-Pena M, Aleman N, Munoz F, Fondevila D, Suarez ML, Goicoa A, Nieto JM. Visceral leishmaniasis with cardiac involvement in a dog: a case report. Acta Vet Scand. 2009 Apr;51(1):1-3. https://doi.org/10.1186/1751-0147-51-20
Nakipoglu D, Ural K. P wave dispersion and cardiac troponin i concentration in canine visceral leishmaniasis. MAE Vet Fak Derg. 2018 Apr 9;3(1):1-16. https://doi.org/10.24880/maeuvfd.306728
Noszczyk-Nowak A, Paslawska U, Szalas A, Nicpon J. P-wave dispersion in healthy dogs. A preliminary study. Bull Vet Inst Pulawy. 2008 Jan 1;52(4):683-8.
Noszczyk-Nowak A, Szalas A, Paslawska U, Nicpon J. Comparison of P-wave dispersion in healthy dogs, dogs with chronic valvular disease and dogs with disturbances of supraventricular conduction. Acta Vet Scand. 2011 Mar 11;53(1):1-6. https://doi.org/10.1186/1751-0147-53-18
Noszczyk-Nowak A. P-wave dispersion in prediction of maintenance of sinus rhythm after an electrical cardioversion of atrial fibrillation in dogs. Bull Vet Inst Pulawy. 2012 Mar;56(1):99-102. https://doi.org/10.2478/v10213-012-0018-0
Okutucu S, Aytemir K, Oto A. P-wave dispersion: What we know till now? JRSM Cardiovasc Dis. 2016 Mar 21;5:2048004016639443. https://doi.org/10.1177/2048004016639443
Paltrinieri S, Gradoni L, Roura X, Zatelli A, Zini E. Laboratory tests for diagnosing and monitoring canine leishmaniasis. Vet Clin Pathol. 2016 Dec;45(4):552-78. https://doi.org/10.1111/vcp.12413
Rosa FA, Leite JHAC, Braga ET, Moreira PRR, Baltazar FH, Biondo AW, Padua PPM, Vasconcelos RO, Camacho AA, Ferreira WL, Machado GF, Marcondes M. Cardiac lesions in 30 dogs naturally infected with Leishmania infantum chagasi. Vet Pathol. 2014 May;51(3):603-6. https://doi.org/10.1177/0300985813493914
Silva VBC, Sousa MG, Araujo CRA, Lima ABG, Carareto R. Cardiac biomarkers in dogs with visceral leishmaniasis. Arch Med Vet. 2016 Jan;48(3):269-75. https://doi.org/10.4067/S0301-732X2016000300004
Solano-Gallego L, Fernandez-Bellon H, Morell P, Fondevila D, Alberola J, Ramis A, Ferrer L. Histological and immunohistochemical study of clinically normal skin of Leishmania infantum-infected dogs. J Comp Pathol. 2004 Jan 1;130(1):7-12. https://doi.org/10.1016/S0021-9975(03)00063-X
Solano-Gallego L, Miro G, Koutinas A, Cardoso L, Pennisi MG, Ferrer L, Baneth G. LeishVet guidelines for the practical management of canine leishmaniosis. Parasit Vectors. 2011 May 20;4(1):86. https://doi.org/10.1186/1756-3305-4-86
Su HM, Tsai WC, Lin TH, Hsu PC, Lee WH, Lin MY, Sheu SH. P wave dispersion and maximum P wave duration are independently associated with rapid renal function decline. PLoS One. 2012 Aug;7(8):e42815. https://doi.org/10.1371/journal.pone.0042815
Ural K, Pasa S, Gultekin M, Balikci C. Interpretation of electrocardiographic, echocardiographic and biochemical findings during different stages of Canine Visceral Leishmaniasis. Rev MVZ Córdoba. 2017 Dec;22(3):6225-40. https://doi.org/10.21897/rmvz.1128
Zaragoza C, Barrera R, Centeno F, Tapia JA, Duran E, Gonzalez M, Mane MC. SDS-PAGE and Western blot of urinary proteins in dogs with leishmaniasis. Vet Res. 2003 Mar-Apr;34(2):137-51. https://doi.org/10.1051/vetres:2002061
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