Seroprevalence of Brucella canis in dogs and at-risk humans in Jordan

https://doi.org/10.17221/67/2018-VETMEDCitation:Alshehabat M., Obaidat M., Hayajneh W. (2019): Seroprevalence of Brucella canis in dogs and at-risk humans in Jordan. Veterinarni Medicina, 64: 260-265.
download PDF

Brucella canis infection is a neglected zoonotic disease and its seroprevalence in dogs and at-risk humans has not been previously studied in several countries including Jordan. The main aim of this study was to determine the seroprevalence and identify risk factors of B. canis infection in police, breeding and stray dogs and in at-risk humans in Jordan. A total of 169 sera samples from apparently healthy dogs and 185 samples from apparently healthy people (85 from dog handlers and 100 from the general population) were tested in the study. Antibodies against B. canis were tested using the canine D-Tec® CB Rapid Slide Agglutination Test (RSAT) kit with secondary 2-mercaptoethanol (ME-RSAT). Overall, 8.3% of the dog sera samples tested positive to antibodies against B. canis, and 37.8% of stray dogs tested positive. Seroprevalence was higher in male dogs than in females. Furthermore, none of the tested human samples was positive to antibodies against B. canis. There was a significant association between seropositivity and the type of dog. The study reports preliminary findings that suggest the presence of Bcanis among stray dogs in Jordan. Thus, preventive measures should be taken to control the transmission of this pathogen from stray dogs to other dogs and humans as well.

References:
Abarca VK, Lopez Del PJ, Pena DA, Lopez JC (2011): Pet ownership and health status of pets from immunocompromised children, with emphasis in zoonotic diseases. Revista Chilena de Infectologia 28, 205–210.
 
Agudelo-Florez P, Castro B, Rojo-Ospina R, Henao-Villegas S (2012): Canine brucellosis: Seroprevalence and risk factors in pets from eleven neighbourhoods in Medellin, Colombia. Revista de Salud Publica 14, 644–656.
 
Angel Martha Olivera, Ristow Paula, Ko Albert Icksang, Di–Lorenzo Cecilia (2012): Serological trail of Brucella infection in an urban slum population in Brazil. The Journal of Infection in Developing Countries, 6, - https://doi.org/10.3855/jidc.2347
 
Comfort Ayoola Modupe, Joseph Ogugua Akwoba, Oluwatoyin Akinseye Victor, Joshua Tunde, Folusho Banuso Morenikeji, Julianah Adedoyin Folashade, Kehinde Adesokan Hezekiah, Olutayo Omobowale Temidayo, Olusoji Abiola John, Ihuaku Otuh Patricia, Oyebukola Nottidge Helen, Dale Emma, Perrett Lorraine, Taylor Andrew, Stack Judy, Idowu Cadmus Simeon (2016): Sero-epidemiological survey and risk factors associated with brucellosis in dogs in south-western Nigeria. Pan African Medical Journal, 23, - https://doi.org/10.11604/pamj.2016.23.29.7794
 
Behzadi MA, Mogheiseh A (2012): Epidemiological survey of Brucella canis infection in 7 different breeds of dogs in Fars Province, Iran. Pakistan Veterinary Journal 32, 234–236.
 
Brower Alexandra, Okwumabua Ogi, Massengill Chuck, Muenks Quentin, Vanderloo Peter, Duster Megan, Homb Kelly, Kurth Kathy (2007): Investigation of the spread of Brucella canis via the U.S. interstate dog trade. International Journal of Infectious Diseases, 11, 454-458 https://doi.org/10.1016/j.ijid.2006.12.009
 
Cadmus S. I. B., Adesokan H. K., Ajala O. O., Odetokun W. O., Perrett L. L., Stack J. A. (2011): Seroprevalence of Brucella abortus and B. canis in household dogs in southwestern Nigeria: a preliminary report. Journal of the South African Veterinary Association, 82, - https://doi.org/10.4102/jsava.v82i1.35
 
Carmichael LE, Zoha SJ, Flores-Castro R (1984): Problems in the serodiagnosis of canine brucellosis: dog responses to cell wall and internal antigens of Brucella canis. Developments in Biological Standardization 56, 371–383.
 
Castrillón-Salazar Laura, Giraldo-Echeverri Carlos Andrés, Sánchez-Jiménez Miryan Margot, Olivera-Angel Martha (2013): Factores asociados con la seropositividad a Brucella canis en criaderos caninos de dos regiones de Antioquia, Colombia. Cadernos de Saúde Pública, 29, 1975-1987 https://doi.org/10.1590/0102-311X00133013
 
Fernandes Annielle Regina Fonseca, Azevedo Sérgio Santos de, Piatti Rosa Maria, Pinheiro Eliana Scarcelli, Genovez Margareth Élide, Azevedo Adílio Santos de, Batista Carolina de Sousa Américo, Alves Clebert José (2011): Brucella canis infection in dogs attended in veterinary clinics from patos, Paraíba state, Brazil. Brazilian Journal of Microbiology, 42, 1405-1408 https://doi.org/10.1590/S1517-83822011000400023
 
Gyuranecz Miklós, Szeredi Levente, Rónai Zsuzsanna, Dénes Béla, Dencso László, Dán Ádám, Pálmai Nimród, Hauser Zsófia, Lami Erzsébet, Makrai László, Erdélyi Károly, Jánosi Szilárd (2011): Detection of Brucella Canis –Induced Reproductive Diseases in a Kennel. Journal of Veterinary Diagnostic Investigation, 23, 143-147 https://doi.org/10.1177/104063871102300127
 
Henderson RA, Hoerlein BF, Kramer TT, Meyer ME (1974): Discospondylitis in three dogs infected with Brucella canis. Journal of the American Veterinary Medical Association 165, 451–455.
 
Kaden Rene, Ågren Joakim, Båverud Viveca, Hallgren Gunilla, Ferrari Sevinc, Börjesson Joann, Lindberg Martina, Bäckman Stina, Wahab Tara (2014): Brucellosis outbreak in a Swedish kennel in 2013: Determination of genetic markers for source tracing. Veterinary Microbiology, 174, 523-530 https://doi.org/10.1016/j.vetmic.2014.10.015
 
Krueger W. S., Lucero N. E., Brower A., Heil G. L., Gray G. C. (2014): Evidence for Unapparent Brucella canis Infections among Adults with Occupational Exposure to Dogs. Zoonoses and Public Health, 61, 509-518 https://doi.org/10.1111/zph.12102
 
LUCERO N. E., CORAZZA R., ALMUZARA M. N., REYNES E., ESCOBAR G. I., BOERI E., AYALA S. M. (2010): Human Brucella canis outbreak linked to infection in dogs. Epidemiology and Infection, 138, 280-285 https://doi.org/10.1017/S0950268809990525
 
AYALA S.M., LOPEZ G., LUCERO N.E., ESCOBAR G.I. (2002): Sensitivity and specificity of an indirect enzyme-linked immunoassay for the diagnosis of Brucella canis infection in dogs. Journal of Medical Microbiology, 51, 656-660 https://doi.org/10.1099/0022-1317-51-8-656
 
Marzetti Sandra, Carranza Cristina, Roncallo Mariela, Escobar Gabriela I., Lucero Nidia E. (2013): Recent trends in human Brucella canis infection. Comparative Immunology, Microbiology and Infectious Diseases, 36, 55-61 https://doi.org/10.1016/j.cimid.2012.09.002
 
Musallam I.I., Abo-Shehada M., Omar M., Guitian J. (2015): Cross-sectional study of brucellosis in Jordan: Prevalence, risk factors and spatial distribution in small ruminants and cattle. Preventive Veterinary Medicine, 118, 387-396 https://doi.org/10.1016/j.prevetmed.2014.12.020
 
Osoro Eric Mogaka, Munyua Peninah, Omulo Sylvia, Ogola Eric, Ade Fredrick, Mbatha Peter, Mbabu Murithi, Ng'ang'a Zipporah, Kairu Salome, Maritim Marybeth, Thumbi Samuel M., Bitek Austine, Gaichugi Stella, Rubin Carol, Njenga Kariuki, Guerra Marta (2015): Strong Association Between Human and Animal Brucella Seropositivity in a Linked Study in Kenya, 2012–2013. The American Journal of Tropical Medicine and Hygiene, 93, 224-231 https://doi.org/10.4269/ajtmh.15-0113
 
Poester Fernando Padilla, Gonçalves Vı́tor Salvador Picão, Lage Andrey Pereira (2002): Brucellosis in Brazil. Veterinary Microbiology, 90, 55-62 https://doi.org/10.1016/S0378-1135(02)00245-6
 
Sayan M, Erdenlig S, Stack J, Kilic S, Guducuoglu H, Aksoy Y, Baklan A, Etiler N (2011): A Serological diagnostic survey for Brucella canis infection in Turkish patients with brucellosis-like symptoms. Japanese Journal of Infectious Diseases 64, 516–519.
 
Wanke Marı́a M, Delpino M.Victoria, Baldi Pablo C (2002): Comparative performance of tests using cytosolic or outer membrane antigens of Brucella for the serodiagnosis of canine brucellosis. Veterinary Microbiology, 88, 367-375 https://doi.org/10.1016/S0378-1135(02)00152-9
 
Wanke M.M., Delpino M.V., Baldi P.C. (2006): Use of enrofloxacin in the treatment of canine brucellosis in a dog kennel (clinical trial). Theriogenology, 66, 1573-1578 https://doi.org/10.1016/j.theriogenology.2006.01.034
 
download PDF

© 2019 Czech Academy of Agricultural Sciences