Comparative study on different field tests of ketosis using blood, milk, and urine in dairy cattle

https://doi.org/10.17221/69/2019-VETMEDCitation:Faruk M.S., Park B., Ha S., Lee S., Mamuad L., Cho Y. (2020): Comparative study on different field tests of ketosis using blood, milk, and urine in dairy cattle. Veterinarni Medicina, 65: 199-206.
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This study assessed the diagnostic performance of five field tests for ketosis in the blood, milk and urine in dairy cows. Samples were collected simultaneously from 102 dairy cows to determine the accuracy of the test results. The concentration of β-hydroxybutyrate (BHBA) in the blood was measured quantitatively by using a portable ketone test kit. The milk and urine samples were analysed semi-quantitatively using different commercial test kits and urine sticks. The animals were categorised into a subclinical ketosis (SCK) and clinical ketosis (CK) group based on the BHBA concentration in the blood. The diagnostic performance of the milk and urine test kits was compared with the blood test kit. In the blood test, out of 102 cows, 27 and 19 cows were diagnosed with SCK and CK, respectively. The percentage agreement (kappa value) for the three different milk tests and the urine test kit was 43% (κ = −0.181), 72% (κ = 0.243), 63% (κ = 0.065) and 64% (κ = 0.163), respectively, for the SCK detection, which indicates lower sensitivity. However, a strong agreement with 88% (κ = 0.612), 85% (κ = 0.505), 86% (κ = 0.528) and 91% (κ = 0.753) was observed in the three milk tests and the urine test, respectively, for the CK diagnosis, showing a higher sensitivity. For the diagnosis of the SCK and CK, all the tested kits are generally applicable for the detection of CK in dairy cattle, but caution is needed during the interpretation of the SCK data due to the low sensitivity of the milk and urine tests for the SCK detection. Furthermore, this study suggests that milk and urine samples can be used as alternatives for the diagnosis of CK in replacement of blood tests.

References:
Al Faruk M, Park BS, Jin SJ, Ha SM, Cho YI. Cross-sectional study: Prevalence of subclinical ketosis in dairy cattle at Chungnam province. Korean J Vet Serv. 2018;41(2):85-9.
 
Andersson L. Subclinical ketosis in dairy cows. Vet Clin North Am Food Anim Pract. 1988 Jul;4(2):233-51. https://doi.org/10.1016/S0749-0720(15)31046-X
 
Carrier J, Stewart S, Godden S, Fetrow J, Rapnicki P. Evaluation and use of three cowside tests for detection of subclinical ketosis in early postpartum cows. J Dairy Sci. 2004 Nov;87(11):3725-35. https://doi.org/10.3168/jds.S0022-0302(04)73511-0
 
Cheruiyot EK, Bett RC, Amimo JO, Mujibi FD. Milk composition for admixed dairy cattle in Tanzania. Front Genet. 2018 Apr 24;9:1-12. https://doi.org/10.3389/fgene.2018.00142
 
Duffield TF, Leslie KE, Sandals D, Lissemore K, Mcbride BW, Lumsden JH, Dick P, Bagg R. Effect of a monensin-controlled release capsule on cow health and reproductive performance. J Dairy Sci. 1999 Nov;82(11):2377-84. https://doi.org/10.3168/jds.S0022-0302(99)75488-3
 
Duffield TF. Subclinical ketosis in lactating dairy cattle. Vet Clin North Am Food Anim Pract. 2000 Jul;16(2):231-53. https://doi.org/10.1016/S0749-0720(15)30103-1
 
Enjalbert F, Nicot M, Bayourthe C, Moncoulon R. Ketone bodies in milk and blood of dairy cows: Relationship between concentrations and utilization for detection of subclinical ketosis. J Dairy Sci. 2001 Mar;84(3):583-9. https://doi.org/10.3168/jds.S0022-0302(01)74511-0
 
Evans M, Cogan KE, Egan B. Metabolism of ketone bodies during exercise and training: Physiological basis for exogenous supplementation. J Physiol. 2017 May 1;595(9):2857-71. https://doi.org/10.1113/JP273185
 
Francos G, Insler G, Dirksen G. Routine testing for milk beta-hydroxybutyrate for the detection of subclinical ketosis in dairy cows. Bovine Pract. 1997;(31/2):61-4.
 
Geishauser T, Leslie K, Kelton D, Duffield T. Evaluation of five cowside tests for use with milk to detect subclinical ketosis in dairy cows. J Dairy Sci. 1998 Feb;81(2):438-43. https://doi.org/10.3168/jds.S0022-0302(98)75595-X
 
Geishauser T, Leslie K, Tenhag J, Bashiri A. Evaluation of eight cow-side ketone tests in milk for detection of subclinical ketosis in dairy cows. J Dairy Sci. 2000 Feb;83(2):296-9. https://doi.org/10.3168/jds.S0022-0302(00)74877-6
 
Ghanem MM, Mahmoud ME, Abd El-Raof YM, El-Attar HM. Efficacy of different cow side tests for diagnosis of ketosis in lactating cows. BVMJ. 2016;31(2):225-30.
 
Goldstein DE, Little RR, Lorenz RA, Malone JI, Nathan D, Peterson CM, Sacks DB. Tests of glycemia in diabetes. Diabetes Care. 2004 Jul;27(7):1761-73. https://doi.org/10.2337/diacare.27.7.1761
 
Grabacka M, Pierzchalska M, Dean M, Reiss K. Regulation of ketone body metabolism and the role of PPARα. Int J Mol Sci. 2016 Dec 13;17(12):1-24. https://doi.org/10.3390/ijms17122093
 
Gustafsson AH, Emanuelson U. Milk acetone concentration as an indicator of hyperketonaemia in dairy cows: The critical value revised. Anim Sci. 1996 Oct;63(2):183-8.  https://doi.org/10.1017/S1357729800014739
 
Jorritsma R, Baldee S, Schukken Y, Wensing T, Wentink G. Evaluation of a milk test for detection of subclinical ketosis. Vet Q. 1998 Jul;20(3):108-10. https://doi.org/10.1080/01652176.1998.9694851
 
Landis JR, Koch GG. The measurement of observer agreement for categorical data. Biometrics. 1977 Mar;33(1):159-74. https://doi.org/10.2307/2529310
 
McArt JAA, Nydam DV, Oetzel GR. Epidemiology of subclinical ketosis in early lactation dairy cattle. J Dairy Sci. 2012 Sep;95(9):5056-66. https://doi.org/10.3168/jds.2012-5443
 
Oetzel GR. Herd-level ketosis – diagnosis and risk factors. Preconference Seminar 7C: Dairy Herd Problem Investigation Strategies: Transition Cow Troubleshooting. Vancouver, BC, Canada: American Association of Bovine Practitioners; 2007. p. 67-91.
 
Sharma A, Srivastava M, Singh A, Ahuja A, Kachhawaha S, Kachhawa J, Daga M, Singh N, Kannojia K. Dipstick urinalysis in ketotic cows. Vet Pract. 2013 Dec;14(2):566-8.
 
Skotnicka E, Muszczynski Z, Dudzinska W, Suska M. A review of the renal system and diurnal variations of renal activity in livestock. Ir Vet J. 2007 Mar 1;60(3):161-8. https://doi.org/10.1186/2046-0481-60-3-161
 
Sullivan KM, Dean A, Soe MM. On academics: OpenEpi: A web-based epidemiologic and statistical calculator for public health. Public Health Rep. 2009 May;124(3):471-4.  https://doi.org/10.1177/003335490912400320
 
Weng X, Zhao W, Neethirajan S, Duffield T. Microfluidic biosensor for β-hydroxybutyrate (βHBA) determination of subclinical ketosis diagnosis. J Nanobiotechnology. 2015 Feb 12;13:1-8. https://doi.org/10.1186/s12951-015-0076-6
 
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