Effects of sodium humate and zinc oxide used in prophylaxis of post-weaning diarrhoea on the health, oxidative stress status and fatty acid profile in weaned piglets

https://doi.org/10.17221/70/2016-VETMEDCitation:Trckova M., Lorencova A., Babak V., Neca J., Ciganek M. (2017): Effects of sodium humate and zinc oxide used in prophylaxis of post-weaning diarrhoea on the health, oxidative stress status and fatty acid profile in weaned piglets. Veterinarni Medicina, 62: 16-28.
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The aim of this study was to investigate the effects of sodium humate (HNa), applied to feed or drinking water as a partial substitution for pharmacological doses of ZnO, on clinical, biochemical and haematological indicators of health status, oxidative stress and the serum fatty acid (FA) profile in weaned piglets. Weaned piglets (32) were allocated into four groups: Control = basal diet containing 110 mg ZnO/kg; ZnO 2.5 = pharmacological dose of ZnO (2.5 g ZnO/kg); ZnO 1.7 + HNa (f) = 1.7 g ZnO and 20.0 g HNa/kg; ZnO 1.7 + HNa (w) = 1.7 g ZnO/kg and drinking water with 0.2% HNa. All ZnO treatments resulted in good performance and clinical health of piglets in contrast to Control, which contained three diarrhoeic piglets. Increased triacylglycerols in the ZnO 2.5 and ZnO 1.7 + HNa (w) groups in comparison with Control suggested increased energy metabolism after treatments. Neither total cholesterol, nor HDL and LDL were affected by treatments. Piglets treated with ZnO and HNa had (ZnO 1.7 + HNa (w)) or tended to have (ZnO 1.7 + HNa (f)) lower urea in serum. Significantly (in ZnO 2.5 and ZnO 1.7 + HNa (f)) or non-significantly (ZnO 1.7 + HNa (w)) higher haematocrit and haemoglobin levels were detected in the blood of treated piglets. Long-term ZnO 2.5 treatment significantly increased serum 8-iso-PGF, the most reliable biomarker of oxidative stress. Partial substitution of ZnO by HNa positively affected the oxidative status of piglets as evidenced by significant (ZnO 1.7 + HNa (w)) or non-significant (ZnO 1.7 + HNa (f)) declines in serum 8-iso-PGF. ZnO 2.5 treatment significantly decreased saturated (SFA), monounsaturated (MUFA) and n-3 polyunsaturated FA (PUFA) and increased PUFA n-6 and n-6/n-3 ratios in serum compared to Control. The synthesis of some physiologically significant long-chain PUFA (LC-PUFA), namely n-6 dihomo-γ-linolenic acid and n-3 eicosapentaenoic, docosapentaenoic was negatively affected by ZnO 2.5 treatment. Both ZnO 1.7 + HNa (f)/(w) treatments significantly increased the proportion of SFA and PUFA n-3 and decreased PUFA n-6 and n-6/n-3 ratios in comparison with ZnO 2.5 and the effect was more considerable in ZnO 1.7 + HNa (w). Most individual PUFA n-3 and n-6 as well as the n-6/n-3 ratio in both treatments were similar to Control. The results indicate that the partial substitution of ZnO by HNa can benefit performance and health of weaned piglets to a similar extent as a high pharmacological dose of ZnO, whereas it can decrease the oxidative stress induced by prolonged over-supplementation of ZnO. Additionally, such a treatment can eliminate the unfavourable effect of high ZnO doses on the n-6/n-3 ratio and the proportion of some physiologically significant LC-PUFA in serum. Generally, it can be concluded that the effects of feed and water HNa supplementation are similar, but are more pronounced when HNa is applied to drinking water.
Alexandre-Pires Graça, de Brito Maria Teresa Villa, Algueró Cármen, Martins Catarina, Rodrigues Olivia Roos, da Fonseca Isabel Pereira, Santos-Gomes Gabriela (2010): Canine leishmaniosis. Immunophenotypic profile of leukocytes in different compartments of symptomatic, asymptomatic and treated dogs. Veterinary Immunology and Immunopathology, 137, 275-283  https://doi.org/10.1016/j.vetimm.2010.06.007
Alvar J, Canavate C, Molina R, Moreno R, Nieto J (2004): Canine leishmaniosis. Advances in Parasitology 57, 1–88.
Amara A, Jemli MH, Kilani M, Ghorbel A, Aouina M (2000): A case of Leishmania nodular dermatitis in a dog (in French). Le Point Veterinaire 31, 514–516.
BLAVIER A., KEROACK S., DENEROLLE Ph., GOY-THOLLOT I., CHABANNE L., CADORÉ J.L., BOURDOISEAU G. (2001): Atypical Forms of Canine Leishmaniosis. The Veterinary Journal, 162, 108-120  https://doi.org/10.1053/tvjl.2000.0556
Bongiorno Gioia, Paparcone Rosa, Manzillo Valentina Foglia, Oliva Gaetano, Cuisinier Anne-Marie, Gradoni Luigi (2013): Vaccination with LiESP/QA-21 (CaniLeish®) reduces the intensity of infection in Phlebotomus perniciosus fed on Leishmania infantum infected dogs—A preliminary xenodiagnosis study. Veterinary Parasitology, 197, 691-695  https://doi.org/10.1016/j.vetpar.2013.05.008
Dye Christopher (1996): The Logic of Visceral Leishmaniasis Control. The American Journal of Tropical Medicine and Hygiene, 55, 125-130  https://doi.org/10.4269/ajtmh.1996.55.125
FERRER L., RABANAL R., FONDEVILA D., RAMOS J. A., DOMINGO M. (1988): Skin lesions in canine leishmaniasis. Journal of Small Animal Practice, 29, 381-388  https://doi.org/10.1111/j.1748-5827.1988.tb02298.x
Ferrer L, Fondevila D, Marco A, Pumarola M (1990): Atypical nodular leishmaniosis in two dogs. Veterinary Record 126, 90.
Handler Marc Z., Patel Parimal A., Kapila Rajendra, Al-Qubati Yasin, Schwartz Robert A. (2015): Cutaneous and mucocutaneous leishmaniasis. Journal of the American Academy of Dermatology, 73, 897-908  https://doi.org/10.1016/j.jaad.2014.08.051
Kahime Kholoud, Boussaa Samia, Idrissi Abderrahmane Laamrani-El, Nhammi Haddou, Boumezzough Ali (2015): Epidemiological study on acute cutaneous leishmaniasis in Morocco. Journal of Acute Disease, , -  https://doi.org/10.1016/j.joad.2015.08.004
KOUTINAS ALEXANDER F., SCOTT DANNY W., KANTOS VASSILIOS, LEKKAS STEFANO (1992): Skin Lesions in Canine Leishmaniasis (Kala-Azar): A Clinical and Histopathological Study on 22 Spontaneous Cases in Greece. Veterinary Dermatology, 3, 121-130  https://doi.org/10.1111/j.1365-3164.1992.tb00158.x
Moreno Javier, Vouldoukis Ioannis, Schreiber Paul, Martin Virginie, McGahie David, Gueguen Sylvie, Cuisinier Anne-Marie (2014): Primary vaccination with the LiESP/QA-21 vaccine (CaniLeish®) produces a cell-mediated immune response which is still present 1 year later. Veterinary Immunology and Immunopathology, 158, 199-207  https://doi.org/10.1016/j.vetimm.2014.01.011
Oliva Gaetano, Nieto Javier, Foglia Manzillo Valentina, Cappiello Silvia, Fiorentino Eleonora, Di Muccio Trentina, Scalone Aldo, Moreno Javier, Chicharro Carmen, Carrillo Eugenia, Butaud Therese, Guegand Laurie, Martin Virginie, Cuisinier Anne-Marie, McGahie David, Gueguen Sylvie, Cañavate Carmen, Gradoni Luigi, Kamhawi Shaden (2014): A Randomised, Double-Blind, Controlled Efficacy Trial of the LiESP/QA-21 Vaccine in Naïve Dogs Exposed to Two Leishmania infantum Transmission Seasons. PLoS Neglected Tropical Diseases, 8, e3213-  https://doi.org/10.1371/journal.pntd.0003213
Otranto Domenico, Dantas-Torres Filipe (2013): The prevention of canine leishmaniasis and its impact on public health. Trends in Parasitology, 29, 339-345  https://doi.org/10.1016/j.pt.2013.05.003
Paltrinieri Saverio, Solano-Gallego Laia, Fondati Alessandra, Lubas George, Gradoni Luigi, Castagnaro Massimo, Crotti Alberto, Maroli Michele, Oliva Gaetano, Roura Xavier, Zatelli Andrea, Zini Eric (2010): Guidelines for diagnosis and clinical classification of leishmaniasis in dogs. Journal of the American Veterinary Medical Association, 236, 1184-1191  https://doi.org/10.2460/javma.236.11.1184
Viegas Carlos, Requicha João, Albuquerque Carlos, Sargo Teresa, Machado João, Dias Isabel, Pires Maria A, Campino Lenea, Cardoso Luís (2012): Tongue nodules in canine leishmaniosis — a case report. Parasites & Vectors, 5, 120-  https://doi.org/10.1186/1756-3305-5-120
Vidor E, Dereure J, Pratlong F, Dubreuil N, Bissuel G, Moreau Y, Rioux JA (1991): The site of inoculation of Leishmania infantum in the canine leishmaniosis. Study of a cohort in traditional Cevennes Region (in French). Pratique Medicale et Chirurgicale de l’Animale de Compagnie 26, 133–137.
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