Hananeh W, Ababneh M (2021): Spotty liver disease in Jordan: An emerging disease. Vet Med-Czech 66, 94–98.
Spotty liver disease is an acute bacterial disease that affects the poultry industry throughout the world. In this report, we discuss the first documented outbreak of the recently emerging disease, spotty liver disease, in a poultry flock in Jordan. The clinical history, pathological and molecular findings are described. The outbreak was characterised by recurrent mortalities that subsided with antibiotic treatments. Grossly, there were multiple pinpoint white foci distributed throughout the enlarged liver and less frequently throughout the spleen too. Histologically, the white foci represented areas of acute hepatocellular lytic necrosis and degeneration that were consistent with those of spotty liver disease. An end point polymerase chain reaction (PCR) assay targeting the glycerol kinase gene, coupled with sequencing, confirmed the pathological diagnosis. Continuous surveillance is needed to estimate the prevalence of this disease in Jordanian poultry flocks.
Campylobacter; liver; poultry; spleen
Burch D. Avian vibrionic hepatitis in laying hens. Vet Rec. 2005 Oct 22;157(17):528. https://doi.org/10.1136/vr.157.17.528-a
Courtice JM, Mahdi LK, Groves PJ, Kotiw M. Spotty liver disease: A review of an ongoing challenge in commercial free-range egg production. Vet Microbiol. 2018 Dec;227:112-8. https://doi.org/10.1016/j.vetmic.2018.08.004
Crawshaw T. A review of the novel thermophilic Campylobacter, Campylobacter hepaticus, a pathogen of poultry. Transbound Emerg Dis. 2019 Jul;66(4):1481-92. https://doi.org/10.1111/tbed.13229
Crawshaw TR, Chanter JI, Young SC, Cawthraw S, Whatmore AM, Koylass MS, Vidal AB, Salguero FJ, Irvine RM. Isolation of a novel thermophilic Campylobacter from cases of spotty liver disease in laying hens and experimental reproduction of infection and microscopic pathology. Vet Microbiol. 2015 Sep 30;179(3-4):315-21.
Di Tommaso P, Moretti S, Xenarios I, Orobitg M, Montanyola A, Chang JM, Taly JF, Notredame C. T-Coffee: A web server for the multiple sequence alignment of protein and RNA sequences using structural information and homology extension. Nucleic Acids Res. 2011 Jul;39(Web Server issue):W13-7.
Grimes T, Reece R. Spotty liver disease – An emerging disease in free range egg layers in Australia. Proceedings of the 60th Western Poultry Disease Conference; 2011 Mar 20-23;Sacramento, CA; 2011. p. 53-6.
Petrovska L, Tang Y, Jansen van Rensburg MJ, Cawthraw S, Nunez J, Sheppard SK, Ellis RJ, Whatmore AM, Crawshaw TR, Irvine RM. Genome reduction for niche association in Campylobacter hepaticus, a cause of spotty liver disease in poultry. Front Cell Infect Microbiol. 2017 Aug 11;7: . Erratum in: Front Cell Infect Microbiol. 2017 Nov 14;7:480. https://doi.org/10.3389/fcimb.2017.00480
Phung C, Vezina B, Anwar A, Wilson T, Scott PC, Moore RJ, Van TTH. Campylobacter hepaticus, the cause of spotty liver disease in chickens: Transmission and routes of infection. Front Vet Sci. 2020 Jan 15;6: . https://doi.org/10.3389/fvets.2019.00505
Scott P, Moore R, Wilson T. Determining the cause and methods of control for spotty liver disease. AECL Publication 2016, No. 1SX091A. North Sydney, NSW: Australian Egg Corporation Limited Wilson; 2016. 58 p.
Scott AB, Singh M, Groves P, Hernandez-Jover M, Barnes B, Glass K, Moloney B, Black A, Toribio JA. Biosecurity practices on Australian commercial layer and meat chicken farms: Performance and perceptions of farmers. PLoS One. 2018 Apr 18;13(4): . https://doi.org/10.1371/journal.pone.0195582
Van TTH, Elshagmani E, Gor MC, Scott PC, Moore RJ. Campylobacter hepaticus sp. nov., isolated from chickens with spotty liver disease. Int J Syst Evol Microbiol. 2016 Nov;66(11):4518-24. https://doi.org/10.1099/ijsem.0.001383
Van TTH, Elshagmani E, Gor MC, Anwar A, Scott PC, Moore RJ. Induction of spotty liver disease in layer hens by infection with Campylobacter hepaticus. Vet Microbiol. 2017a Feb;199:85-90. https://doi.org/10.1016/j.vetmic.2016.12.033
Van TTH, Gor MC, Anwar A, Scott PC, Moore RJ. Campylobacter hepaticus, the cause of spotty liver disease in chickens, is present throughout the small intestine and caeca of infected birds. Vet Microbiol. 2017b Aug;207:226-30.