Association between ESR1 and RBP4 genes and litter size traits in a hyperprolific line of Landrace × Large White cross sows

https://doi.org/10.17221/87/2018-VETMEDCitation:Mencik S., Vukovic V., Spehar M., Modric M., Ostovic M., Ekert Kabalin A. (2019): Association between ESR1 and RBP4 genes and litter size traits in a hyperprolific line of Landrace × Large White cross sows. Veterinarni Medicina, 64: 109-117.
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This study was aimed at analysing single-nucleotide polymorphisms in the oestrogen receptor 1 (ESR1) and retinol-binding protein 4 (RBP4) genes in a hyperprolific line of Landrace × Large White (Topigs 20) cross sows (n = 101). The following litter size traits were analysed: total number born, number of born alive and number of weaned piglets. ESR1 and RBP4 genotypes determined on the basis of single-nucleotide polymorphisms were analysed using the least square method with the GLM procedure in SAS with eight effects. The REG procedure was used to calculate the effects of the additive and dominance components. The second parity sows with ESR1 BB genotype had a significantly higher (P < 0.05) number of weaned piglets compared to AB, with a tendency towards difference (P < 0.1) between homozygotes for number of born alive and number of weaned piglets. In the case of the RBP4 gene, the first parity sows of the AA genotype had a significantly higher total number born (P < 0.05) compared with the BB genotype, with a tendency towards difference (P < 0.1) between AA and heterozygotes for total number born, and homozygotes for number of born alive. The BB genotype showed a tendency for higher number of weaned piglets (P < 0.1) as compared with the AA genotype in the third parity sows for the RBP4 gene. In all parities, significant effects (P < 0.05) of parity were recorded for total number born, number of born alive and number of weaned piglets, season of farrowing for total number born, and the ESR1 and RBP4 interaction for number of born alive. In the first parity sows, significant effects (P < 0.05) on total number born were determined for gene interaction and gestation length, the latter also being recorded in the second parity sows. The additive (a) effect of single-nucleotide polymorphisms in RBP4 was significant (P < 0.05) for total number born in all parities as well as in the first parity sows, and dominance effect (d) (P < 0.05) of single-nucleotide polymorphisms in ESR1 for number of weaned piglets in the third parity sows. The obtained results regarding the investigated genes could help to provide a better understanding of the effect of single-nucleotide polymorphisms on litter size and thus promote genetic progress in pig reproduction management.

References:
Alfonso Leopoldo (2005): Use of meta-analysis to combine candidate gene association studies: application to study the relationship between the ESR PvuII polymorphism and sow litter size. Genetics Selection Evolution, 37, 417- https://doi.org/10.1186/1297-9686-37-5-417
 
Santana Bárbara Amélia Aparecida, Biase Fernando H., Antunes Robson Carlos, Borges Mauricio, Franco Mauricio Machaim, Goulart Luiz Ricardo (2006): Association of the estrogen receptor gene Pvu II restriction polymorphism with expected progeny differences for reproductive and performance traits in swine herds in Brazil. Genetics and Molecular Biology, 29, 273-277 https://doi.org/10.1590/S1415-47572006000200013
 
Bergfelder-Drüing Sarah, Grosse-Brinkhaus Christine, Lind Bianca, Erbe Malena, Schellander Karl, Simianer Henner, Tholen Ernst, Chen Lin (2015): A Genome-Wide Association Study in Large White and Landrace Pig Populations for Number Piglets Born Alive. PLOS ONE, 10, e0117468- https://doi.org/10.1371/journal.pone.0117468
 
Bondesson Maria, Hao Ruixin, Lin Chin-Yo, Williams Cecilia, Gustafsson Jan-Åke (2015): Estrogen receptor signaling during vertebrate development. Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 1849, 142-151 https://doi.org/10.1016/j.bbagrm.2014.06.005
 
Chvojková Z., Hraška Š. (2008): Changes in Reproductive Traits of Large White Pigs after Estrogen Receptor Gene-based Selection in Slovakia: Preliminary Results. Asian-Australasian Journal of Animal Sciences, 21, 320-324 https://doi.org/10.5713/ajas.2008.70252
 
Clop A, Nyman G, Sanchez A, Noguera JL, Andersson L (2000): Detection of the phylogenetic origin by pyrosequencing a cytochrome B region in mitochondrial DNA in different Spanish pig breeds (in Spanish). ITEA Produccion Animal 96, 220–224.
 
Dall´Ollio S, Fontanesi L, Tognazzi L, Buttazzoni L, Gallo M, Russo V (2011): ESR1 and ESR2 gene markers are not associated with number of piglets born alive in Italian Large White sows. Italian Journal of Animal Science 10, 185–188.
 
Dever Joseph T., Surles Rebecca L., Davis Christopher R., Tanumihardjo Sherry A. (2011): α-Retinol Is Distributed through Serum Retinol-Binding Protein-Independent Mechanisms in the Lactating Sow-Nursing Piglet Dyad. The Journal of Nutrition, 141, 42-47 https://doi.org/10.3945/jn.110.127597
 
Distl O (2007): Mechanisms of Regulation of Litter Size in Pigs on the Genome Level. Reproduction in Domestic Animals, 42, 10-16 https://doi.org/10.1111/j.1439-0531.2007.00887.x
 
Drogemuller C, Hamann H, Distl O (2001): Candidate gene markers for litter size in different German pig lines.. Journal of Animal Science, 79, 2565- https://doi.org/10.2527/2001.79102565x
 
Goliášová E., Wolf J. (2004): Impact of the ESR gene on litter size and production traits in Czech Large White pigs. Animal Genetics, 35, 293-297 https://doi.org/10.1111/j.1365-2052.2004.01155.x
 
Hernandez Lopez SH, Lemus Flores C, Morales RA, Herrera Haro JG (2006): Effect of candidate genes on reproductive traits of sows. Revista Cientifica 16, 648–654.
 
Horogh G., Zsolnai A., Komlosi I., Nyiri A., Anton I., Fesus L. (2005): Oestrogen receptor genotypes and litter size in Hungarian Large White pigs. Journal of Animal Breeding and Genetics, 122, 56-61 https://doi.org/10.1111/j.1439-0388.2004.00483.x
 
Humpolicek P, Tvrdon Z, Urban T (2009): Interaction of ESR1 gene with the FSHB and MYOG genes: effect on the reproduction and growth in pigs. Animal Science Papers and Reports 27, 105–113.
 
Isler B. J., Irvin K. M., Neal S. M., Moeller S. J., Davis M. E. (2002): Examination of the relationship between the estrogen receptor gene and reproductive traits in swine. Journal of Animal Science, 80, 2334- https://doi.org/10.2527/2002.8092334x
 
Kapelański Wojciech, Eckert Robert, Jankowiak Hanna, Mucha Aurelia, Bocian Maria, Grajewska Salomea (2013): Polymorphism of ESR, FSHß, RBP4, PRL, OPN genes and their influence on morphometric traits of gilt reproductive tract before sexual maturity. Acta Veterinaria Brno, 82, 369-374 https://doi.org/10.2754/avb201382040369
 
Kernerová N., Matoušek V., Čermáková A., Forbelská M. (2009): Role of genetic markers in the prediction of classification of Czech Large White gilts to a hyperprolific line. Archives Animal Breeding, 52, 40-50 https://doi.org/10.5194/aab-52-40-2009
 
Kmiec M, Dvorak J, Vrtkova I (2002): Study on a relation between estrogen receptor (ESR) gene polymorphism and some pig reproduction performance characters in Polish Landrace breed. Czech Journal of Animal Science 47, 189–193.
 
Korwin-Kossakowska A, Kapelanski W, Bocian M, Kamyczek M, Sender G (2005): Preliminary study of RBP4, EGF and PTGS2 gene polymorphism in pigs and its association with reproductive traits of sows. Animal Science Papers and Reports 23, 95–105.
 
Krupa E., Wolf J. (2013): Simultaneous estimation of genetic parameters for production and litter size traits in Czech Large White and Czech Landrace pigs. Czech Journal of Animal Science, 58, 429-436 https://doi.org/10.17221/6943-CJAS
 
Liu SF, Du LW, Yan YC (2002): Relationship between PvuII polymorphisms at estrogen receptor gene and litter size in swine. Hereditas 24, 267–270.
 
Marantidis A., Laliotis G. P., Avdi M. (2016): Association of RBP4 Genotype with Phenotypic Reproductive Traits of Sows. Genetics Research International, 2016, 1-5 https://doi.org/10.1155/2016/4940532
 
Matousek V, Kernerova N, Kolarikova O, Krizova H, Urban T, Vrtkova I (2003): Effect of RYR1 and ESR genotypes on the fertility of sows of Large White breed in elite herds. Czech Journal of Animal Science 48, 129–133.
 
Mencik S, Vukovic V, Modric M, Spehar M, Ostovic M, Susic V, Stokovic I, Salajpal K, Kabalin H, Ekert Kabalin A (2016): The effect of estrogen receptor genotypes on the number of stillborn and mummified piglets in Topigs 20 sows. Veterinarski arhiv 86, 311–322.
 
Muñoz María, Fernández Ana Isabel, Óvilo Cristina, Muñoz Gloria, Rodriguez Carmen, Fernández Almudena, Alves Estefânia, Silió Luis (2010): Non-additive effects of RBP4, ESR1 and IGF2 polymorphisms on litter size at different parities in a Chinese-European porcine line. Genetics Selection Evolution, 42, - https://doi.org/10.1186/1297-9686-42-23
 
Nagy I., Farkas J., Curik I., Gorjanc G., Gyovai P., Szendrő Zs. (2014): Estimation of additive and dominance variance for litter size components in rabbits. Czech Journal of Animal Science, 59, 182-189 https://doi.org/10.17221/7342-CJAS
 
Noguera J.L., Varona L., Gómez-Raya L., Sánchez A., Babot D., Estany J., Messer L.A., Rothschild M., Pérez-Enciso M. (2003): Estrogen receptor polymorphism in Landrace pigs and its association with litter size performance. Livestock Production Science, 82, 53-59 https://doi.org/10.1016/S0301-6226(03)00004-6
 
Omelka R, Martiniakova M, Peskovicova D, Bauerova M (2008): Associations between RBP4/MspI polymorphism and reproductive traits in pigs: an application of animal model. Journal of Agrobiology 25, 77–80.
 
Rempel L. A., Nonneman D. J., Wise T. H., Erkens T., Peelman L. J., Rohrer G. A. (2010): Association analyses of candidate single nucleotide polymorphisms on reproductive traits in swine1,2. Journal of Animal Science, 88, 1-15 https://doi.org/10.2527/jas.2009-1985
 
Rodriguez Santiago, Gaunt Tom R., Day Ian N. M. (2009): Hardy-Weinberg Equilibrium Testing of Biological Ascertainment for Mendelian Randomization Studies. American Journal of Epidemiology, 169, 505-514 https://doi.org/10.1093/aje/kwn359
 
Rothschild Max F., Messer Lori, Day Andy, Wales Richard, Short Tom, Southwood Olwen, Plastow Graham (2000): Investigation of the retinol-binding protein 4 (RBP4) gene as a candidate gene for increased litter size in pigs. Mammalian Genome, 11, 75-77 https://doi.org/10.1007/s003350010015
 
Short T H, Rothschild M F, Southwood O I, McLaren D G, de Vries A, van der Steen H, Eckardt G R, Tuggle C K, Helm J, Vaske D A, Mileham A J, Plastow G S (1997): Effect of the estrogen receptor locus on reproduction and production traits in four commercial pig lines.. Journal of Animal Science, 75, 3138- https://doi.org/10.2527/1997.75123138x
 
Spötter A, Müller S, Hamann H, Distl O (2009): Effect of Polymorphisms in the Genes for LIF and RBP4 on Litter Size in Two German Pig Lines. Reproduction in Domestic Animals, 44, 100-105 https://doi.org/10.1111/j.1439-0531.2007.01004.x
 
Terman A, Kmiec M, Polasik D, Pradziadowicz K (2007): Retinol binding protein 4 gene and reproductive traits in pigs. Archives fuer Tierzucht Dummerstorf 50, 181–185.
 
Terman A, Kmiec M, Polasik D, Rybarczyk A (2011): Association between RBP4 gene polymorphism and reproductive traits in Polish sows. Journal of Animal and Veterinary Advances 10, 2639–2641.
 
Gonçalves Iara Denise Vasconcellos, Gonçalves Paulo Bayard Dias, Silva Janandra Cortese da, Portela Jr Valério Valdetar, Borges Luiz Felipe Kruel, Oliveira João Francisco Coelho, Lovatto Paulo Alberto (2008): Interaction between estrogen receptor and retinol-binding protein-4 polymorphisms as a tool for the selection of prolific pigs. Genetics and Molecular Biology, 31, 481-486 https://doi.org/10.1590/S1415-47572008000300014
 
Vitezica Zulma G., Varona Luis, Elsen Jean-Michel, Misztal Ignacy, Herring William, Legarra Andrès (2016): Genomic BLUP including additive and dominant variation in purebreds and F1 crossbreds, with an application in pigs. Genetics Selection Evolution, 48, - https://doi.org/10.1186/s12711-016-0185-1
 
Wang X., Wang A., Fu J., Lin H. (2006): Effects of ESR1, FSHB and RBP4 genes on litter size in a Large White and a Landrace Herd. Archives Animal Breeding, 49, 64-70 https://doi.org/10.5194/aab-49-64-2006
 
WU Zhen-Fang, LIU De-Wu, WANG Qing-Lai, ZENG Hai-Yu, CHEN Yao-Shen, ZHANG Hao (2006): Study on the Association Between Estrogen Receptor Gene (ESR) and Reproduction Traits in Landrace Pigs. Acta Genetica Sinica, 33, 711-716 https://doi.org/10.1016/S0379-4172(06)60103-0
 
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