The molecular and morphometric identification of Dictyocaulus capreolus in clinically affected roe deer (Capreolus capreolus L.) J., Jirsova D., Pafco B., Forejtek P. (2019): The molecular and morphometric identification of Dictyocaulus capreolus in clinically affected roe deer (Capreolus capreolus L.). Veterinarni Medicina, 64: 386-391.
download PDF

The poor state of health and increased mortality rate of young roe deer, as reported by South Moravian hunters, caused by the increasing numbers of adult nematodes in the lungs of roe deer prompted us to identify the parasites using a combination of morphological measurements and a phylogenetic SSU rRNA analysis. The study was conducted in a 294 ha game reserve in South Moravia, Czech Republic. Molecular and morphometric techniques were used to identify adult nematodes collected from the respiratory tracts of nine 4–5 months old roe deer in poor health (low body weight of 3–4 kg, poor haircoat quality, and, in some cases, symptoms of diarrhoea). The morphological identification was based on a combination of adult worm characteristics corresponding to Dictyocaulus capreolus. A small subunit rRNA (SSU) partial sequence analysis showed the highest identity scores (99%) corresponding to the sequences of D. capreolus from a roe deer (GenBank: AY168859) from Sweden and the outcomes of the phylogenetic analyses resulted in a tree with a high branch support for two groups, with our sequences forming a well-supported clade with D. capreolus and Dictyocaulus sp. ex Capreolus capreolus (FJ589016) and Dictyocaulus sp. ex Rupicapra rupicapra (FJ589019) sequences from Spain. The examined roe deer have shown symptoms of diarrhoea, anorexia, and respiratory tract inflammation indicating that there might be a connection to the clinical importance of the Dictyocaulus infection.

Aguirre A, Brojer C, Morner T (1999): Descriptive epidemiology of roe deer mortality in Sweden. Journal of Wildlife Diseases 35, 753–762.
Carreno RA, Diez-Banos N, del Rosario Hidalgo-Arguello M, Nadler SA (2009): Characterisation of Dictyocaulus species (Nematoda: Trichostrongyloidea) from three species of wild ruminants. Journal of Parasitology 95, 966–970.
Chilton NB, Huby-Chilton F, Gasser RB (2003): First complete large subunit ribosomal RNA sequence and secondary structure for a parasitic nematode: phylogenetic and diagnostic implication. Molecular and Cellular Probes 17, 33–39.
Christensson D, Rehbinder C (1975): Parasites in reindeer calves--faeces examination (In Swedish). Nordic Veterinary Medicine 27, 496–498.
Divina BP, Wilhelmsson E, Mattsson J, Waller PJ, Hoglund J (2000): Identification of Dictyocalus spp. in ruminants by comparative morphological and molecular analysis. Parasitology 121, 193–201.
Durette-Desset MC, Hugonnet L, Chabaud AG (1988): Redescription of Dictyocaulus noerneri Railliet et Henry, 1907, a parasite of Capreolus capreolus in Europe, comparison with D. viviparus (Bloch, 1782), a parasite of cattle (In French). Annales de Parasitologie Humaine et Comparée 63, 285–295.
Enigk K, Hildebrandt J (1965): Host specificity of Dictyocaulus-species in ruminants. Veterinary Medicine Review 2, 80–97.
Epe C, Samson-Himmelstjerna GV, Schneider T (1997): Differences in a ribosomal DNA sequence of lungworm species (Nematoda: Dictyocaulidae) from fallow deer, cattle, sheep and donkeys. Research in Veterinary Science 62, 17–21.
Gibbons LM, Hoglund J (2002): Dictyocaulus capreolus n. sp. (Nematoda: Trichostrongylidea) from roe deer, Capreolus capreolus and moose Alces alces in Sweden. Journal of Helminthology 76, 119–124.
Guildal JA (1962): Endoparasites of Danish red deer (Cervus elaphus L.) and of Danish fallow deer (Dama dama L.). Contributions to the parasitic fauna of Denmark. Kongelige Veterinaerog Lanbo-hejskole Aarskrift 1, 49–61.
Guindon S, Gascuel, O (2003): A simple, fast and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology 52, 696–704.
Hoglund J, Wilhelmsson E, Christensson D, Waller P, Mattsson JG (1999): ITS2 sequences of Dictyocaulus species from cattle, roe deer and moose in Sweden: molecular evidence for a new species. International Journal of Parasitology 29, 607–611.
Hoglund J, Morrison DA, Divina, BP, Wilhelmsson E, Mattsson JG (2003): Phylogeny of Dictyocaulus (lungworms) from eight species of ruminants based on analyses of ribosomal RNA data. Parasitology 127, 179–187.
Jansen J, Borgsteede FH (1990): Dictyocaulus species, lungworms in cattle and deer in The Netherlands. Tijdschrift voor Diergeneeskunde 115, 155–158.
Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Meintjes P, Drummond A (2012): Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28, 1647–1649.
Nilsson O (1971): The inter-relationship of endoparasites in wild cervids (Capreolus capreolus L, and Alces alces L.) and domestic ruminants. Acta Veterinaria Scandinavica 12, 36–68.
Nylander JAA, Wilgenbusch JC, Warren DL, Swofford DL (2008): AWTY (are we there yet?): a system for graphical exploration of MCMC convergence in Bayesian phylogenetics. Bioinformatics 24, 581–583.
Page RD (1996): TreeView: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12, 357–358.
Posada D (2008): jModelTest: Phylogenetic model averaging. Molecular Biology and Evolution 25, 1253–1256.
Pyziel AM (2014): Molecular analysis of lungworms from European bison (Bison bonasus) on the basis of small subunit ribosomal RNA gene (SSU). Acta Parasitologica 59, 122–125.
Pyziel AM, Laskowski Z, Demiaszkiewicz AW, Hoglund J (2017): Interrelationships of Dictyocaulus spp. In wild ruminants with morphological description of Dictyocaulus cervi n.sp. (Nematoda: Trichostrongyloidea) from red deer, Cervus elaphus. Journal of Parasitology 103, 506–518.
Pyziel AM, Dolka I, Werszko J, Laskowski Z, Steiner-Bogdaszewska Z, Wisniewski J, Demiaszkiewicz AW, Anusz K (2018): Pathological lesions in the lungs of red deer, Cervus elaphus (L.) induced by a newly-described Dictyocaulus cervi (Nematoda: Trichostrongyloidea). Veterinary Parasitology, 261, 22–26.
Ronquist F, Huelsenbeck JP (2003): MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572–1574.
Rubin R, Lucker JT (1956): The course of pathogenicity of initial infections with Dictyocaulus viviparus the lungworm of cattle. American Journal of Veterinary Research 17, 217–226.
Schneider T, Kaup FJ, Drommer W (1991): Morphological investigation on the pathology of Dictyocaulus viviparus infection in cattle. Parasitology Research 77, 260–265.
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti