Random amplified polymorphic DNA (RAPD) analysis of Pasteurella multocida and Manheimia haemolytica strains isolated from cattle, sheep and goats

https://doi.org/10.17221/5678-VETMEDCitation:Ozbey G., Kilic A., B Ertas H., Muz A. (2004): Random amplified polymorphic DNA (RAPD) analysis of Pasteurella multocida and Manheimia haemolytica strains isolated from cattle, sheep and goats. Veterinarni Medicina, 49: 65-69.
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n this study, 30, 15 and 1 strains of <i>Pasteurella multocida</i> and 9, 8 and 6 strains of <i>Mannheimia haemolytica</i> from cattle, sheep and goats isolated in Elazig province located in the East of Turkey, respectively were typed by random amplified polymorphic DNA (RAPD) assay using a random primer (OPA-11). By RAPD assay, two and three distinct band profiles were obtained in the examination of <i>P. multocida</i> isolates from cattle and sheep, respectively. However, <i>M. haemolytica</i> isolates from cattle, sheep and goats showed only one profile and these strains were not discriminated by RAPD. This study showed that little genetic heterogeneity exists among <i>P. multocida</i> and <i>M. haemolytica</i> isolates from lungs of cattle, sheep and goats.
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