Cryoprotection of porcine cornea: a scanning electron microscopy study
A. Neronov, P. Giurov, M. Cholakova, M. Dimitrova, E. Nikolovahttps://doi.org/10.17221/5618-VETMEDCitation:Neronov A., Giurov P., Cholakova M., Dimitrova M., Nikolova E. (2005): Cryoprotection of porcine cornea: a scanning electron microscopy study . Veterinarni Medicina, 50: 219-224.
Porcine corneas were frozen with Me2SO, glycerol, 1,2-propanediol and PEG-400. The effects of the range of concentrations (5% and 10%) and temperature regimen (1ºC/min and 5ºC/min) were investigated. The integrity of corneal endothelial cells was evaluated by scanning electron microscopy and trypan blue staining. The presence of 5–10% PEG-400 in the protective medium was the most effective in minimizing changes in the integrity of the corneal endothelium during freezing-thawing procedures.Keywords:
porcine cornea; Me2SO; glycerol; 1,2-propanediol; PEG-400