γδ-TCR+ CD2– lymphocytes are recruited into bovine mammary gland after stimulation
M. Faldyna, L. Leva, Z. Sladek, D. Rysanek, M. Tomanhttps://doi.org/10.17221/5545-VETMEDCitation:Faldyna M., Leva L., Sladek Z., Rysanek D., Toman M. (2006): γδ-TCR+ CD2– lymphocytes are recruited into bovine mammary gland after stimulation. Veterinarni Medicina, 51: 258-264.
γδ-T-Cell Receptor (TCR) lymphocytes were detected in mammary gland lavages collected from 10 clinically healthy virgin heifers before and after intramammary stimulation with synthetic muramyl dipeptide analogue. Using two-colour flow cytometry, CD2+ and CD2– subsets of γδ-TCR lymphocytes were analyzed. CD2+ γδ-TCR lymphocytes markedly prevailed over CD2– cells in intact mammary gland: 88.9 ± 4.9% of γδ-TCR lymphocytes were CD2+. After stimulation, neutrophils and γδ-TCR lymphocytes were recruited into the mammary gland. Among γδ-TCR lymphocytes, CD2– cells were mainly responsible for their expansion. After stimulation, 60.8 ± 13.4% of γδ-TCR lymphocytes were CD2+ (P < 0.01 when compared with mammary gland lavages before stimulation). It follows from the present study that the cells seem to be involved in the first phase of a response to an infection affecting mammary gland.Keywords:
cattle; udder; mastitis