Appearance of iron-labeled blood mononuclear cells in electron microscopy
D. Horky, I. Lauschova, M. Klabusay, M. Doubek, P. Sheer, S. Palsa, J. Doubekhttps://doi.org/10.17221/5525-VETMEDCitation:Horky D., Lauschova I., Klabusay M., Doubek M., Sheer P., Palsa S., Doubek J. (2006): Appearance of iron-labeled blood mononuclear cells in electron microscopy. Veterinarni Medicina, 51: 89-92.
Mononuclear cells from rabbit bone marrow were cultured for 14 days in cell-free medium for hematopoietic cells together with iron oxid nanoparticles, and then they were processed by technique for free cells for TEM (transmission electron microscopy). Staining with turnbull blue was used for the detection of iron using a light microscope. It was shown that iron nanoparticles were incorporated into the cytoplasm of mononuclear cells during 14 days cultivation. Here they were localized within different sized vacuoles with distinct membranes.Keywords:
ultrastructure; iron nanoparticles; cultivation; animal model