Immunohistological changes in skin wounds during the early periods of healing in a rat model
F. Sabol, L. Dancakova, P. Gal, T. Vasilenko, M. Novotny, K. Smetana, L. Lenhardthttps://doi.org/10.17221/5253-VETMEDCitation:Sabol F., Dancakova L., Gal P., Vasilenko T., Novotny M., Smetana K., Lenhardt L. (2012): Immunohistological changes in skin wounds during the early periods of healing in a rat model. Veterinarni Medicina, 57: 77-82.
The complexity of the wound healing process, which is still poorly understood, prompted us to perform an immunohistochemical investigation using rat skin as an in vivo model. Fifteen Sprague-Dawley rats were included in the experiment. Two round full thickness wounds, 4 mm in diameter, were made on the backs of all rats. Haematoxylin and eosin basic staining as well as antibodies against wide spectrum keratin, keratin 10, keratin 14, α-smooth muscle actin, vimentin, fibronectin, collagens Type 1 and 3, and the transcription factor Sox-2 were applied to paraffin and frozen sections of skin wound specimens two, six and fourteen days after surgery, respectively. New hair follicles with Sox-2-positive cells were present after fourteen days; keratin/vimentin positivity was restricted to specimens of day two. Collagen-3 expression prevailed over collagen-1 expression at all evaluated time intervals, except in the uninjured part of the dermis. In conclusion, rat skin wound healing is a dynamic process which can serve as a model for studying phenomena such as cell-cell interactions and transitions in vivo.Keywords:
tissue repair and regeneration; cell differentiation; transition; proliferation; wound healing