Co-infection of bovine papillomavirus type-1 and -10 in teat warts of dairy cattle
P. Kumar, BL Jangir, G. Saikumar, R. Somvanshihttps://doi.org/10.17221/7178-VETMEDCitation:Kumar P., Jangir B., Saikumar G., Somvanshi R. (2013): Co-infection of bovine papillomavirus type-1 and -10 in teat warts of dairy cattle. Veterinarni Medicina, 58: 605-608.
The present study was carried out to investigate the involvement of different bovine papillomaviruses in the teat warts of cattle. A total of 11 teat wart samples showing rice grain-like and small, sessile elevated greyish or flesh-like growths were collected from dairy cattle. DNA was extracted from these teat wart samples and PCR and real time PCR techniques were applied using specific primers for BPV-1 and -10 to detect the presence of viral nucleic acid. PCR revealed the presence of viral DNA of BPV-1 and -10 in three and seven samples, respectively. Quantification using real time PCR revealed that the copy numbers of the viral DNA of BPV-1 and -10 DNA varied from 1.12E + 04 to 2.99E + 04 and 3.56E + 02 to 5.23E + 06, respectively. From the present study it can be concluded that BPV-1 and -10 are involved in production of rice grain-like and sessile elevated growths on the teats of cattle.Keywords:
teat wart; bovine papillomavirus; cattle, PCR; real time PCR