The genotoxicity of caecal water from gilts following experimentally induced Fusarium mycotoxicosis

https://doi.org/10.17221/8058-VETMEDCitation:Nowak A., Slizewska K., Gajecka M., Piotrowska M., Zakowska Z., Zielonka L., Gajecki M. (2015): The genotoxicity of caecal water from gilts following experimentally induced Fusarium mycotoxicosis. Veterinarni Medicina, 60: 133-140.
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The examination of caecal contents allows one to investigate the exposure of colon mucosa to dietary toxins and the chemical contaminants present in animal feed. The objective of the present study was to examine the influence of Fusarium mycotoxins (zearalenone, ZEN, and deoxynivalenol, DON), administered separately and as a mixture, on the genotoxicity of caecal water (CW) from gilts fed these mycotoxins. CW genotoxicity was evaluated with the comet assay using the LLC-PK1 porcine epithelial kidney cell line. It was shown that after the first week of the experiment, the presence of DON in animal feed led to increased CW genotoxicity in the proximal colon, while the presence of DON and ZEN + DON had a similar effect in the distal colon (ANOVA, P < 0.05). After the third week of experiment, elevated genotoxicity in the distal colon was observed for all experimental groups of gilts, and it was 62%, 52.4%, and 52.8% higher for ZEN-, DON-, and ZEN + DON-fed animals, respectively, than for control group animals. However, no effect on CW genotoxicity in the proximal colon was seen. After six weeks, the presence of ZEN in the proximal colon increased CW genotoxicity by 103% in comparison with the control group. In the distal colon, after the sixth week, CW genotoxicity in all groups of animals fed with mycotoxin was significantly (by 80% to 116%) higher than in the control group. ZEN and DON administered as a mixture did not lead to an increased genotoxicity compared to either agent administered separately. Generally, the mycotoxins clearly started to increase the genotoxicity of CW from the third week of administration.
References:
Antonissen Gunther, Martel An, Pasmans Frank, Ducatelle Richard, Verbrugghe Elin, Vandenbroucke Virginie, Li Shaoji, Haesebrouck Freddy, Immerseel Filip, Croubels Siska (2014): The Impact of Fusarium Mycotoxins on Human and Animal Host Susceptibility to Infectious Diseases. Toxins, 6, 430-452  https://doi.org/10.3390/toxins6020430
 
Basso Karina, Gomes Fernando, Bracarense Ana (2013): Deoxynivanelol and Fumonisin, Alone or in Combination, Induce Changes on Intestinal Junction Complexes and in E-Cadherin Expression. Toxins, 5, 2341-2352  https://doi.org/10.3390/toxins5122341
 
Błasiak Janusz, Kowalik Joanna (2000): A comparison of the in vitro genotoxicity of tri- and hexavalent chromium. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 469, 135-145  https://doi.org/10.1016/S1383-5718(00)00065-6
 
Boermans Herman J., Leung Maxwell C.K. (2007): Mycotoxins and the pet food industry: Toxicological evidence and risk assessment. International Journal of Food Microbiology, 119, 95-102  https://doi.org/10.1016/j.ijfoodmicro.2007.07.063
 
Cortinovis Cristina, Pizzo Fabiola, Spicer Leon J., Caloni Francesca (2013): Fusarium mycotoxins: Effects on reproductive function in domestic animals—A review. Theriogenology, 80, 557-564  https://doi.org/10.1016/j.theriogenology.2013.06.018
 
Döll Susanne, Dänicke Sven (2011): The Fusarium toxins deoxynivalenol (DON) and zearalenone (ZON) in animal feeding. Preventive Veterinary Medicine, 102, 132-145  https://doi.org/10.1016/j.prevetmed.2011.04.008
 
Gajecki M, Gajecka M, Jakimiuk E, Zielonka L, Obremski K (2010): Zearalenone – undesirable substance. In: Rai M., Ajit V (eds.): Mycotoxins in Food, Feed and Bioweapons. Springer-Verlag, Berlin-Heidelberg. 131–144.
 
Gutleb Arno C., Morrison Ellen, Murk Albertinka J. (2002): Cytotoxicity assays for mycotoxins produced by Fusarium strains: a review. Environmental Toxicology and Pharmacology, 11, 309-320  https://doi.org/10.1016/S1382-6689(02)00020-0
 
Klaude Maria, Eriksson Stefan, Nygren Jonas, Ahnström Gunnar (1996): The comet assay: mechanisms and technical considerations. Mutation Research/DNA Repair, 363, 89-96  https://doi.org/10.1016/0921-8777(95)00063-1
 
Klinder Annett, Karlsson Pernilla C., Clune Yvonne, Hughes Roisin, Glei Michael, Rafter Joseph J., Rowland Ian, Collins John K., Pool-Zobel Beatrice L. (2007): Fecal Water as a Non-Invasive Biomarker in Nutritional Intervention: Comparison of Preparation Methods and Refinement of Different Endpoints. Nutrition and Cancer, 57, 158-167  https://doi.org/10.1080/01635580701274848
 
Laufersweiler Michael C., Gadagbui Bernard, Baskerville-Abraham Irene M., Maier Andrew, Willis Alison, Scialli Anthony R., Carr Gregory J., Felter Susan P., Blackburn Karen, Daston George (2012): Correlation of chemical structure with reproductive and developmental toxicity as it relates to the use of the threshold of toxicological concern. Regulatory Toxicology and Pharmacology, 62, 160-182  https://doi.org/10.1016/j.yrtph.2011.09.004
 
Luongo D., Severino L., Bergamo P., De Luna R., Lucisano A., Rossi M. (2006): Interactive effects of fumonisin B1 and α-zearalenol on proliferation and cytokine expression in Jurkat T cells. Toxicology in Vitro, 20, 1403-1410  https://doi.org/10.1016/j.tiv.2006.06.006
 
Ma Yi Yi, Guo Hong Wei (2008): Mini-review of studies on the carcinogenicity of deoxynivalenol. Environmental Toxicology and Pharmacology, 25, 1-9  https://doi.org/10.1016/j.etap.2007.09.007
 
Malekinejad H., Maas-Bakker R., Fink-Gremmels J. (2006): Species differences in the hepatic biotransformation of zearalenone. The Veterinary Journal, 172, 96-102  https://doi.org/10.1016/j.tvjl.2005.03.004
 
Obremski K, Gajecki M, Zwierzchowski W, Bakula T, Apoznanski J, Wojciechowski J (2003): The level of zearalenone and α-zearalenol in the blood of gilts after feeding them of feed with a low content of zearalenone. Journal of Animal Feed Science 12, 529–538.
 
Obremski K, Zielonka L, Gajecka M, Jakimiuk E, Bakula T, Baranowski M, Gajecki M (2005): Histological estimation of the small intestine walll aftrer administration of feed containing deoxynivalenol, T-2 toxin and zearalenone in the pig. Polish Journal of Veterinary Sciences 11, 339–345.
 
Pearson Jennifer R, Gill Chris IR, Rowland Ian R (2009): Diet, fecal water, and colon cancer - development of a biomarker. Nutrition Reviews, 67, 509-526  https://doi.org/10.1111/j.1753-4887.2009.00224.x
 
Pinton Philippe, Oswald Isabelle (2014): Effect of Deoxynivalenol and Other Type B Trichothecenes on the Intestine: A Review. Toxins, 6, 1615-1643  https://doi.org/10.3390/toxins6051615
 
Piotrowska Małgorzata, Śliżewska Katarzyna, Nowak Adriana, Zielonka Łukasz, Żakowska Zofia, Gajęcka Magdalena, Gajęcki Maciej (2014): The Effect of Experimental Fusarium Mycotoxicosis on Microbiota Diversity in Porcine Ascending Colon Contents. Toxins, 6, 2064-2081  https://doi.org/10.3390/toxins6072064
 
Rohweder Dirk, Kersten Susanne, Valenta Hana, Sondermann Sarah, Schollenberger Margit, Drochner Winfried, Dänicke Sven (2013): Bioavailability of the Fusarium toxin deoxynivalenol (DON) from wheat straw and chaff in pigs. Archives of Animal Nutrition, 67, 37-47  https://doi.org/10.1080/1745039X.2012.755328
 
Savard Christian, Pinilla Vicente, Provost Chantale, Segura Mariela, Gagnon Carl A., Chorfi Younes (2014): In vitro effect of deoxynivalenol (DON) mycotoxin on porcine reproductive and respiratory syndrome virus replication. Food and Chemical Toxicology, 65, 219-226  https://doi.org/10.1016/j.fct.2013.12.043
 
Schlatter Josef (2004): Toxicity data relevant for hazard characterization. Toxicology Letters, 153, 83-89  https://doi.org/10.1016/j.toxlet.2004.04.025
 
Schoevers Eric J., Santos Regiane R., Colenbrander Ben, Fink-Gremmels Johanna, Roelen Bernard A.J. (2012): Transgenerational toxicity of Zearalenone in pigs. Reproductive Toxicology, 34, 110-119  https://doi.org/10.1016/j.reprotox.2012.03.004
 
Singh Narendra P., McCoy Michael T., Tice Raymond R., Schneider Edward L. (1988): A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research, 175, 184-191  https://doi.org/10.1016/0014-4827(88)90265-0
 
Sobrova Pavlina, Adam Vojtech, Vasatkova Anna, Beklova Miroslava, Zeman Ladislav, Kizek Rene (2010): Deoxynivalenol and its toxicity. Interdisciplinary Toxicology, 3, -  https://doi.org/10.2478/v10102-010-0019-x
 
Tiemann U., Dänicke S. (2007): In vivo and in vitro effects of the mycotoxins zearalenone and deoxynivalenol on different non-reproductive and reproductive organs in female pigs: A review. Food Additives and Contaminants, 24, 306-314  https://doi.org/10.1080/02652030601053626
 
Waché Yann J., Valat Charlotte, Postollec Gilbert, Bougeard Stephanie, Burel Christine, Oswald Isabelle P., Fravalo Philippe (2009): Impact of Deoxynivalenol on the Intestinal Microflora of Pigs. International Journal of Molecular Sciences, 10, 1-17  https://doi.org/10.3390/ijms10010001
 
Wan Lam Yim Murphy, Turner Paul C., El-Nezami Hani (2013): Individual and combined cytotoxic effects of Fusarium toxins (deoxynivalenol, nivalenol, zearalenone and fumonisins B1) on swine jejunal epithelial cells. Food and Chemical Toxicology, 57, 276-283  https://doi.org/10.1016/j.fct.2013.03.034
 
Waśkiewicz Agnieszka, Beszterda Monika, Kostecki Marian, Zielonka Łukasz, Goliński Piotr, Gajęcki Maciej (2014): Deoxynivalenol in the Gastrointestinal Tract of Immature Gilts under per os Toxin Application. Toxins, 6, 973-987  https://doi.org/10.3390/toxins6030973
 
Zwierzchowski W, Gajecki M, Obremski K., Zielonka L., Baranowski M (2004): The occurrence of zearalenone and its derivatives in standard and therapeutic feeds for companion animals. Polish Journal in Veterinary Science 7, 289–293.
 
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