Antimicrobial effects of topical skin cream containing natural oil mixtures against Staphylococcus pseudintermedius and Malassezia pachydermatis
JI Han, SJ Park, SG Kim, HM Parkhttps://doi.org/10.17221/8108-VETMEDCitation:Han J., Park S., Kim S., Park H. (2015): Antimicrobial effects of topical skin cream containing natural oil mixtures against Staphylococcus pseudintermedius and Malassezia pachydermatis. Veterinarni Medicina, 60: 202-207.
The objective of this study was to evaluate the in vitro efficacy of a topical skin cream containing a mixture of emu oil, jojoba oil, avocado oil, and tea tree oil against the canine skin pathogens Staphylococcus pseudintermedius and Malassezia pachydermatis. Three S. pseudintermedius isolates from dogs and a type strain of M. pachydermatis were used. Based on the standards of the Clinical and Laboratory Standards Institute, the minimal inhibitory concentration (MIC) and the minimal bactericidal/fungicidal concentration (MBC/MFC) were determined. In addition, microbial inactivation time was determined for both pathogens. The MICs against S. pseudintermedius and M. pachydermatis were 0.23% and 0.63%, while the MBC/MFCs were 7.5% and 5%, respectively. In assessments of the microbial inactivation time, after 12 h of incubation with the cream, the growth of both pathogens was completely inhibited. These results suggest that the skin cream tested here can be used as a substitute for generally used antibiotic/antifungal agents.Keywords:natural oil; Staphylococcus pseudintermedius; Malassezia pachydermatis; antimicrobial effectReferences:
Abimosleh SM, Tran CD, Howarth GS (2012): Emu oil: a novel therapeutic for disorders of the gastrointestinal tract? Journal of Gastroenterology and Hepatology 27, 857–861.Bennett Darin C., Code William E., Godin David V., Cheng Kimberly M. (2008): Comparison of the antioxidant properties of emu oil with other avian oils. Australian Journal of Experimental Agriculture, 48, 1345- https://doi.org/10.1071/EA08134Brown MA, Craig-Schmidt MC, Smith PC (1995): Fatty acid composition of emu. Informs 6, 470.Cafarchia C., Gallo S., Romito D., Capelli G., Chermette R., Guillot J., Otranto D. (2005): Frequency, Body Distribution, and Population Size of Malassezia Species in Healthy Dogs and in Dogs with Localized Cutaneous Lesions. Journal of Veterinary Diagnostic Investigation, 17, 316-322 https://doi.org/10.1177/104063870501700403Carson C. F., Hammer K. A., Riley T. V. (): Melaleuca alternifolia (Tea Tree) Oil: a Review of Antimicrobial and Other Medicinal Properties. Clinical Microbiology Reviews, 19, 50-62 https://doi.org/10.1128/CMR.19.1.50-62.2006Chaurasia SC, Vyas KK (1977): In vitro effect of some volatile oil against phytophthoraparasitica vr. Piperina. Journal of Research and Education in Indian Medicine. Yoga and Homeopathy 24–26.Escher M., Vanni M., Intorre L., Caprioli A., Tognetti R., Scavia G. (): Use of antimicrobials in companion animal practice: a retrospective study in a veterinary teaching hospital in Italy. Journal of Antimicrobial Chemotherapy, 66, 920-927 https://doi.org/10.1093/jac/dkq543Ross Fitzgerald J. (2009): The Staphylococcus intermedius group of bacterial pathogens: species re-classification, pathogenesis and the emergence of meticillin resistance. Veterinary Dermatology, 20, 490-495 https://doi.org/10.1111/j.1365-3164.2009.00828.xFREIRE MARCELO MOREIRA, JHAM GULAB NEWANDRAM, DHINGRA ONKAR DEV, JARDIM CAROLINA MARANGON, BARCELOS ROSIMEIRE COURA, VALENTE VÂNIA MARIA MOREIRA (2012): COMPOSITION, ANTIFUNGAL ACTIVITY AND MAIN FUNGITOXIC COMPONENTS OF THE ESSENTIAL OIL OF MENTHA PIPERITA L.. Journal of Food Safety, 32, 29-36 https://doi.org/10.1111/j.1745-4565.2011.00341.xKadlec K, Schwarz S (2013): Antimicrobial resistance of Staphylococcus pseudintermedius. Veterinary Dermatology 23, 276–282, e55.LaPlante K. L., Mermel L. A. (2007): In vitro activity of daptomycin and vancomycin lock solutions on staphylococcal biofilms in a central venous catheter model. Nephrology Dialysis Transplantation, 22, 2239-2246 https://doi.org/10.1093/ndt/gfm141Moon Therese, Wilkinson Jenny M., Cavanagh Heather M. A. (2006): Antiparasitic activity of two Lavandula essential oils against Giardia duodenalis, Trichomonas vaginalis and Hexamita inflata. Parasitology Research, 99, 722-728 https://doi.org/10.1007/s00436-006-0234-8Neu H. C. (1992): The Crisis in Antibiotic Resistance. Science, 257, 1064-1073 https://doi.org/10.1126/science.257.5073.1064Politis M. J., Dmytrowich A. (1998): Promotion of Second Intention Wound Healing by Emu Oil Lotion: Comparative Results with Furasin, Polysporin, and Cortisone. Plastic & Reconstructive Surgery, 102, 2404-2407 https://doi.org/10.1097/00006534-199812000-00020Prabussenivasan S, Jayakumar M, Ignacimuthu S (2006): In vitro antibacterial activity of some plant essential oils. BMC Complementary and Alternative Medicine 6, 39–46. https://doi.org/10.1186/1472-6882-6-39Sasaki T., Tsubakishita S., Tanaka Y., Sakusabe A., Ohtsuka M., Hirotaki S., Kawakami T., Fukata T., Hiramatsu K. (): Multiplex-PCR Method for Species Identification of Coagulase-Positive Staphylococci. Journal of Clinical Microbiology, 48, 765-769 https://doi.org/10.1128/JCM.01232-09Valentine BK, Dew W, Yu A, Weese JS (2012): In vitro evaluation of topical biocide and antimicrobial susceptibility of Staphylococcus pseudintermedius from dogs. Veterinary Dermatology 23, 493, e95.Weseler A., Geiss H.K., Saller R., Reichling J. (2002): Antifungal effect of Australian tea tree oil on Malassezia pachydermatis isolated from canines suffering from cutaneous skin disease. Schweizer Archiv für Tierheilkunde, 144, 215-221 https://doi.org/10.1024/0036-72126.96.36.199Williams DG (1997): The chemistry of essential oils: An introduction for aromatherapists, beauticians, retailers and students. 1st ed. Micelle Press, Dorset, UK.Wu Shuhua, Patel Krupa B, Booth J. Leland, Metcalf Jordan P, Lin Hsueh-Kung, Wu Wenxin (2010): Protective Essential Oil Attenuates Influenza Virus Infection: An In Vitro Study In MDCK Cells. BMC Complementary and Alternative Medicine, 10, 69- https://doi.org/10.1186/1472-6882-10-69Yoganathan Subbiah, Nicolosi Robert, Wilson Thomas, Handelman Garry, Scollin Patrick, Tao Richard, Binford Paul, Orthoefer Frank (2003): Antagonism of croton oil inflammation by topical emu oil in CD-1 mice. Lipids, 38, 603-607 https://doi.org/10.1007/s11745-003-1104-y