Cloprostenol and eCG influence oestrus synchronisation and uterine development in mice

https://doi.org/10.17221/7923-VETMEDCitation:Wei S., Gong Z., An L., Zhang T., Luo Y., Dai H. (2015): Cloprostenol and eCG influence oestrus synchronisation and uterine development in mice. Veterinarni Medicina, 60: 31-38.
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The aim of this study was to investigate the efficacy of equine chorionic gonadotropin (eCG) and cloprostenol (CLO) administration on oestrus synchronisation, uterine development as well as serum LH and FSH concentrations in mice. One hundred and five KM mice were assigned into eCG-1, eCG-2, eCG-3, CLO-1, CLO-2, CLO-3 and control groups (CG, n = 15). The eCG-1, eCG-2 and eCG-3 groups were intramuscularly injected with 10, 20 and 40 IU eCG. CLO-1, CLO-2 and CLO-3 were intramuscularly injected with 10, 15 and 20 μg cloprostenol acetate. The results showed that 93.33% and 66.67% of synchronised mice displayed oestrus within 18.68–37.59 h. Oestrus numbers, oestrus onset time (EOT) and oestrus rate in CLO and eCG groups were greater than in CG (P < 0.05). EOT in CLO and eCG groups were 19.88 ± 2.91 h and 34.84 ± 5.05 h. Uterine weights of treatment groups were larger than CG. Uterine weights of the eCG group were higher than those in the CLO group. Uterine horn longitudial diameters (ULD) in treatment groups were larger than CG during the experiment. ULD in eCG-2 and eCG-3 were significantly greater when compared to CG (P < 0.05 or P < 0.01). On days 14 and 21, uterine horn transverse diameters (UTD) in CLO-1, eCG-1, eCG-2 and eCG-3 subgroups were significantly larger than that of CG (P < 0.05 or P < 0.01). Serum LH concentrations in eCG and CLO increased. Increments in eCG and CLO groups were greater than that of CG. FSH concentrations in eCG mice were higher than those in CLO and CG mice (P < 0.05) on day 21. Thus, eCG and cloprostenol treatments in mice can improve uterine development and promote the secretion of LH and FSH.
References:
Abecia José A., Forcada Fernando, González-Bulnes Antonio (2011): Pharmaceutical Control of Reproduction in Sheep and Goats. Veterinary Clinics of North America: Food Animal Practice, 27, 67-79  https://doi.org/10.1016/j.cvfa.2010.10.001
 
Abecia J.A., Forcada F., González-Bulnes A. (2012): Hormonal control of reproduction in small ruminants. Animal Reproduction Science, 130, 173-179  https://doi.org/10.1016/j.anireprosci.2012.01.011
 
Arbeiter K, Arbeiter E (1985): Alfaprostol for the induction of ovulation during estrus in the mare (in German). Deutsche Tieraerztliche Wochenschrift 92, 87–88.
 
Bunger L, Schuler L, Renne U, Kupatz B (1982): Selection and growth of laboratory mice under synchronized estrus conditions. Direct selection success and correlated selection effect on litter size. Archiv fur Experimentelle Veterinarmedizin 36, 641–646.
 
Chia Chun-Chieh, Huang Soon-Cen, Chen Shen-Shien, Kang Jye-Yi, Lin Jing-Chuan, Lin Yuen-Shan, Huang Kuo-Feng, Lee Hung-Jung, Zheng Ching-Cheng (2006): Ultrasonographic Evaluation of the Change in Uterine Fibroids Induced by Treatment with a Gnrh Analog. Taiwanese Journal of Obstetrics and Gynecology, 45, 124-128  https://doi.org/10.1016/S1028-4559(09)60209-6
 
Cordova-Izquierdo A, Ruiz LG, Saltijeral OJ, Perez GJF, Degefa DT (1999): Induction and synchronisation of estrus in anoestric Creole ewes with FGA impregnated vaginal sponges and PMSG injection (in Spanish). Archivos de Zootechnia 48, 437–440.
 
Cordova-Izquierdo A, Xolalpa-Campos VM, Ruiz-Lang CG, Espinosa-Cervantes R, Cordova-Jimenez CA, Mendez-Mendoza M, Huerta-Crispin R, Villa-Mancera A, Juarez-Mosqueda ML, Sanchez-Aparicio P, Guerra-Liera E (2012): Effect of cloprostenol and fluorogestone acetate more pmsg on synchronization and no return to estrus in seasonal anestrus dorper sheep. Journal of Applied Sciences Research 3, 1612–1614.
 
Crawford J L, Heath D A, Haydon L J, Thomson B P, Eckery D C (): Gene expression and secretion of LH and FSH in relation to gene expression of GnRH receptors in the brushtail possum (Trichosurus vulpecula) demonstrates highly conserved mechanisms. Reproduction, 137, 129-140  https://doi.org/10.1530/REP-08-0347
 
Cuervo-Arango J, Newcombe JR (2010): Cloprostenol in equine reproductive practice: something more than a luteolytic drug. Reproduction in Domestic Animals 45, e8–e11.
 
De Rensis F, López-Gatius F (2014): Use of Equine Chorionic Gonadotropin to Control Reproduction of the Dairy Cow: A Review. Reproduction in Domestic Animals, 49, 177-182  https://doi.org/10.1111/rda.12268
 
Dezhkam Y, Sadrkhanlou R (2009): Study of PMSG and HCG effects on mice uterus morphology in preimplantation period. International Journal of Gynecology and Obstetrics 107 (Suppl. 2), S154–S155.
 
Fu W, Sun Y, Suo L, Gao J (2005): Observation of estrous cycle and choice of optimal superovulation time in mice. Journal of Beijing Agricultural College 20, 19–21.
 
Girsh Eliezer, Plaks Vicki, Gilad Assaf A., Nevo Nava, Schechtman Edna, Neeman Michal, Dekel Nava (2007): Cloprostenol, a prostaglandin F2α analog, induces hypoxia in rat placenta: BOLD contrast MRI. NMR in Biomedicine, 20, 28-39  https://doi.org/10.1002/nbm.1087
 
Gravina FS, van Helden DF, Kerr KP, de Oliveira RB, Jobling P (2014): Phasic contractions of the mouse vagina and cervix at different phases of the estrus cycle and during late pregnancy. PLoS One 9: e111307.
 
Kirkwood RN, De Rensis F, Silva P, Gherpelli M, Alegre B, Dominguez JC (2007): Effect of Cloprostenol Treatment at Artificial Insemination on Sow Fertility. Reproduction in Domestic Animals, 42, 26-28  https://doi.org/10.1111/j.1439-0531.2006.00717.x
 
Malakoff D. (): SUPPLIERS:The Rise of the Mouse, Biomedicine's Model Mammal. Science, 288, 248-253  https://doi.org/10.1126/science.288.5464.248
 
Pallares P., Gonzalez-Bulnes A. (2009): A new method for induction and synchronization of oestrus and fertile ovulations in mice by using exogenous hormones. Laboratory Animals, 43, 295-299  https://doi.org/10.1258/la.2008.008056
 
Schuler L (1977): Selection for fertility under gestagen synchronization of estrus in mice. Genetika 13, 840–846.
 
Uslu Baris Atalay, Tasal Ibrahim, Gulyuz Fetih, Sendag Sait, Ucar Omer, Goericke-Pesch Sandra, Wehrend Axel (2012): Effects of oestrus synchronisation using melatonin and norgestomet implants followed by eCG injection upon reproductive traits of fat-tailed Morkaraman ewes during suckling, anoestrus season. Small Ruminant Research, 108, 102-106  https://doi.org/10.1016/j.smallrumres.2012.07.002
 
Ye F, Shi Y, Chen H, Zhang D, Qin C, Shi Y (2010): Experiment study of estrus synchronization for different ewe breeds in non-breeding seasons. Herbivore of China 30, 45–47.
 
Yuan L, Wang B, Piao S, Tan J, An T (2008): Effect of PMSG on development of reproductive organs in different day-old immature mice. China Journal of Experimental Animal 16, 338–341.
 
Zhang C (2010): Germplasm resources protection and development for Lanzhou fat-tailed sheep. Animal Husbandry Journal of China 46, 7–10.
 
Zhang C, Liu L, Di M, Zhao D, Zhao L, Xu P, Rui R (2007): Effects of the PMSG dosages on superovulation and oocyte quality in immature rats. Chinese Journal of Compararive Medicine 16, 338–344.
 
Zhou J (1995): Research on estrus synchronization and superovulation in KM mice (in Chinese). Chinese Journal of Experimental Animal Science 5, 139–141.
 
ZHU Na, JIA Hong-Xiang, LIU Xiao-Kun, ZHAO Xiao-E, WEI Qiang, MA Bao-Hua (2012): Measuring the estrus cycle and its effect on superovulation in mice. Zoological Research, 33, 276-282  https://doi.org/10.3724/SP.J.1141.2012.03276
 
Abecia José A., Forcada Fernando, González-Bulnes Antonio (2011): Pharmaceutical Control of Reproduction in Sheep and Goats. Veterinary Clinics of North America: Food Animal Practice, 27, 67-79  https://doi.org/10.1016/j.cvfa.2010.10.001
 
Abecia J.A., Forcada F., González-Bulnes A. (2012): Hormonal control of reproduction in small ruminants. Animal Reproduction Science, 130, 173-179  https://doi.org/10.1016/j.anireprosci.2012.01.011
 
Arbeiter K, Arbeiter E (1985): Alfaprostol for the induction of ovulation during estrus in the mare (in German). Deutsche Tieraerztliche Wochenschrift 92, 87–88.
 
Bunger L, Schuler L, Renne U, Kupatz B (1982): Selection and growth of laboratory mice under synchronized estrus conditions. Direct selection success and correlated selection effect on litter size. Archiv fur Experimentelle Veterinarmedizin 36, 641–646.
 
Chia Chun-Chieh, Huang Soon-Cen, Chen Shen-Shien, Kang Jye-Yi, Lin Jing-Chuan, Lin Yuen-Shan, Huang Kuo-Feng, Lee Hung-Jung, Zheng Ching-Cheng (2006): Ultrasonographic Evaluation of the Change in Uterine Fibroids Induced by Treatment with a Gnrh Analog. Taiwanese Journal of Obstetrics and Gynecology, 45, 124-128  https://doi.org/10.1016/S1028-4559(09)60209-6
 
Cordova-Izquierdo A, Ruiz LG, Saltijeral OJ, Perez GJF, Degefa DT (1999): Induction and synchronisation of estrus in anoestric Creole ewes with FGA impregnated vaginal sponges and PMSG injection (in Spanish). Archivos de Zootechnia 48, 437–440.
 
Cordova-Izquierdo A, Xolalpa-Campos VM, Ruiz-Lang CG, Espinosa-Cervantes R, Cordova-Jimenez CA, Mendez-Mendoza M, Huerta-Crispin R, Villa-Mancera A, Juarez-Mosqueda ML, Sanchez-Aparicio P, Guerra-Liera E (2012): Effect of cloprostenol and fluorogestone acetate more pmsg on synchronization and no return to estrus in seasonal anestrus dorper sheep. Journal of Applied Sciences Research 3, 1612–1614.
 
Crawford J L, Heath D A, Haydon L J, Thomson B P, Eckery D C (): Gene expression and secretion of LH and FSH in relation to gene expression of GnRH receptors in the brushtail possum (Trichosurus vulpecula) demonstrates highly conserved mechanisms. Reproduction, 137, 129-140  https://doi.org/10.1530/REP-08-0347
 
Cuervo-Arango J, Newcombe JR (2010): Cloprostenol in equine reproductive practice: something more than a luteolytic drug. Reproduction in Domestic Animals 45, e8–e11.
 
De Rensis F, López-Gatius F (2014): Use of Equine Chorionic Gonadotropin to Control Reproduction of the Dairy Cow: A Review. Reproduction in Domestic Animals, 49, 177-182  https://doi.org/10.1111/rda.12268
 
Dezhkam Y, Sadrkhanlou R (2009): Study of PMSG and HCG effects on mice uterus morphology in preimplantation period. International Journal of Gynecology and Obstetrics 107 (Suppl. 2), S154–S155.
 
Fu W, Sun Y, Suo L, Gao J (2005): Observation of estrous cycle and choice of optimal superovulation time in mice. Journal of Beijing Agricultural College 20, 19–21.
 
Girsh Eliezer, Plaks Vicki, Gilad Assaf A., Nevo Nava, Schechtman Edna, Neeman Michal, Dekel Nava (2007): Cloprostenol, a prostaglandin F2α analog, induces hypoxia in rat placenta: BOLD contrast MRI. NMR in Biomedicine, 20, 28-39  https://doi.org/10.1002/nbm.1087
 
Gravina FS, van Helden DF, Kerr KP, de Oliveira RB, Jobling P (2014): Phasic contractions of the mouse vagina and cervix at different phases of the estrus cycle and during late pregnancy. PLoS One 9: e111307.
 
Kirkwood RN, De Rensis F, Silva P, Gherpelli M, Alegre B, Dominguez JC (2007): Effect of Cloprostenol Treatment at Artificial Insemination on Sow Fertility. Reproduction in Domestic Animals, 42, 26-28  https://doi.org/10.1111/j.1439-0531.2006.00717.x
 
Malakoff D. (): SUPPLIERS:The Rise of the Mouse, Biomedicine's Model Mammal. Science, 288, 248-253  https://doi.org/10.1126/science.288.5464.248
 
Pallares P., Gonzalez-Bulnes A. (2009): A new method for induction and synchronization of oestrus and fertile ovulations in mice by using exogenous hormones. Laboratory Animals, 43, 295-299  https://doi.org/10.1258/la.2008.008056
 
Schuler L (1977): Selection for fertility under gestagen synchronization of estrus in mice. Genetika 13, 840–846.
 
Uslu Baris Atalay, Tasal Ibrahim, Gulyuz Fetih, Sendag Sait, Ucar Omer, Goericke-Pesch Sandra, Wehrend Axel (2012): Effects of oestrus synchronisation using melatonin and norgestomet implants followed by eCG injection upon reproductive traits of fat-tailed Morkaraman ewes during suckling, anoestrus season. Small Ruminant Research, 108, 102-106  https://doi.org/10.1016/j.smallrumres.2012.07.002
 
Ye F, Shi Y, Chen H, Zhang D, Qin C, Shi Y (2010): Experiment study of estrus synchronization for different ewe breeds in non-breeding seasons. Herbivore of China 30, 45–47.
 
Yuan L, Wang B, Piao S, Tan J, An T (2008): Effect of PMSG on development of reproductive organs in different day-old immature mice. China Journal of Experimental Animal 16, 338–341.
 
Zhang C (2010): Germplasm resources protection and development for Lanzhou fat-tailed sheep. Animal Husbandry Journal of China 46, 7–10.
 
Zhang C, Liu L, Di M, Zhao D, Zhao L, Xu P, Rui R (2007): Effects of the PMSG dosages on superovulation and oocyte quality in immature rats. Chinese Journal of Comparative Medicine 16, 338–344.
 
Zhou J (1995): Research on estrus synchronization and superovulation in KM mice (in Chinese). Chinese Journal of Experimental Animal Science 5, 139–141.
 
ZHU Na, JIA Hong-Xiang, LIU Xiao-Kun, ZHAO Xiao-E, WEI Qiang, MA Bao-Hua (2012): Measuring the estrus cycle and its effect on superovulation in mice. Zoological Research, 33, 276-282  https://doi.org/10.3724/SP.J.1141.2012.03276
 
Abecia José A., Forcada Fernando, González-Bulnes Antonio (2011): Pharmaceutical Control of Reproduction in Sheep and Goats. Veterinary Clinics of North America: Food Animal Practice, 27, 67-79  https://doi.org/10.1016/j.cvfa.2010.10.001
 
Abecia J.A., Forcada F., González-Bulnes A. (2012): Hormonal control of reproduction in small ruminants. Animal Reproduction Science, 130, 173-179  https://doi.org/10.1016/j.anireprosci.2012.01.011
 
Arbeiter K, Arbeiter E (1985): Alfaprostol for the induction of ovulation during estrus in the mare (in German). Deutsche Tieraerztliche Wochenschrift 92, 87–88.
 
Bunger L, Schuler L, Renne U, Kupatz B (1982): Selection and growth of laboratory mice under synchronized estrus conditions. Direct selection success and correlated selection effect on litter size. Archiv fur Experimentelle Veterinarmedizin 36, 641–646.
 
Chia Chun-Chieh, Huang Soon-Cen, Chen Shen-Shien, Kang Jye-Yi, Lin Jing-Chuan, Lin Yuen-Shan, Huang Kuo-Feng, Lee Hung-Jung, Zheng Ching-Cheng (2006): Ultrasonographic Evaluation of the Change in Uterine Fibroids Induced by Treatment with a Gnrh Analog. Taiwanese Journal of Obstetrics and Gynecology, 45, 124-128  https://doi.org/10.1016/S1028-4559(09)60209-6
 
Cordova-Izquierdo A, Ruiz LG, Saltijeral OJ, Perez GJF, Degefa DT (1999): Induction and synchronisation of estrus in anoestric Creole ewes with FGA impregnated vaginal sponges and PMSG injection (in Spanish). Archivos de Zootechnia 48, 437–440.
 
Cordova-Izquierdo A, Xolalpa-Campos VM, Ruiz-Lang CG, Espinosa-Cervantes R, Cordova-Jimenez CA, Mendez-Mendoza M, Huerta-Crispin R, Villa-Mancera A, Juarez-Mosqueda ML, Sanchez-Aparicio P, Guerra-Liera E (2012): Effect of cloprostenol and fluorogestone acetate more pmsg on synchronization and no return to estrus in seasonal anestrus dorper sheep. Journal of Applied Sciences Research 3, 1612–1614.
 
Crawford J L, Heath D A, Haydon L J, Thomson B P, Eckery D C (): Gene expression and secretion of LH and FSH in relation to gene expression of GnRH receptors in the brushtail possum (Trichosurus vulpecula) demonstrates highly conserved mechanisms. Reproduction, 137, 129-140  https://doi.org/10.1530/REP-08-0347
 
Cuervo-Arango J, Newcombe JR (2010): Cloprostenol in equine reproductive practice: something more than a luteolytic drug. Reproduction in Domestic Animals 45, e8–e11.
 
De Rensis F, López-Gatius F (2014): Use of Equine Chorionic Gonadotropin to Control Reproduction of the Dairy Cow: A Review. Reproduction in Domestic Animals, 49, 177-182  https://doi.org/10.1111/rda.12268
 
Dezhkam Y, Sadrkhanlou R (2009): Study of PMSG and HCG effects on mice uterus morphology in preimplantation period. International Journal of Gynecology and Obstetrics 107 (Suppl. 2), S154–S155.
 
Fu W, Sun Y, Suo L, Gao J (2005): Observation of estrous cycle and choice of optimal superovulation time in mice. Journal of Beijing Agricultural College 20, 19–21.
 
Girsh Eliezer, Plaks Vicki, Gilad Assaf A., Nevo Nava, Schechtman Edna, Neeman Michal, Dekel Nava (2007): Cloprostenol, a prostaglandin F2α analog, induces hypoxia in rat placenta: BOLD contrast MRI. NMR in Biomedicine, 20, 28-39  https://doi.org/10.1002/nbm.1087
 
Gravina FS, van Helden DF, Kerr KP, de Oliveira RB, Jobling P (2014): Phasic contractions of the mouse vagina and cervix at different phases of the estrus cycle and during late pregnancy. PLoS One 9: e111307.
 
Kirkwood RN, De Rensis F, Silva P, Gherpelli M, Alegre B, Dominguez JC (2007): Effect of Cloprostenol Treatment at Artificial Insemination on Sow Fertility. Reproduction in Domestic Animals, 42, 26-28  https://doi.org/10.1111/j.1439-0531.2006.00717.x
 
Malakoff D. (): SUPPLIERS:The Rise of the Mouse, Biomedicine's Model Mammal. Science, 288, 248-253  https://doi.org/10.1126/science.288.5464.248
 
Pallares P., Gonzalez-Bulnes A. (2009): A new method for induction and synchronization of oestrus and fertile ovulations in mice by using exogenous hormones. Laboratory Animals, 43, 295-299  https://doi.org/10.1258/la.2008.008056
 
Schuler L (1977): Selection for fertility under gestagen synchronization of estrus in mice. Genetika 13, 840–846.
 
Uslu Baris Atalay, Tasal Ibrahim, Gulyuz Fetih, Sendag Sait, Ucar Omer, Goericke-Pesch Sandra, Wehrend Axel (2012): Effects of oestrus synchronisation using melatonin and norgestomet implants followed by eCG injection upon reproductive traits of fat-tailed Morkaraman ewes during suckling, anoestrus season. Small Ruminant Research, 108, 102-106  https://doi.org/10.1016/j.smallrumres.2012.07.002
 
Ye F, Shi Y, Chen H, Zhang D, Qin C, Shi Y (2010): Experiment study of estrus synchronization for different ewe breeds in non-breeding seasons. Herbivore of China 30, 45–47.
 
Yuan L, Wang B, Piao S, Tan J, An T (2008): Effect of PMSG on development of reproductive organs in different day-old immature mice. China Journal of Experimental Animal 16, 338–341.
 
Zhang C (2010): Germplasm resources protection and development for Lanzhou fat-tailed sheep. Animal Husbandry Journal of China 46, 7–10.
 
Zhang C, Liu L, Di M, Zhao D, Zhao L, Xu P, Rui R (2007): Effects of the PMSG dosages on superovulation and oocyte quality in immature rats. Chinese Journal of Comparative Medicine 16, 338–344.
 
Zhou J (1995): Research on estrus synchronization and superovulation in KM mice (in Chinese). Chinese Journal of Experimental Animal Science 5, 139–141.
 
ZHU Na, JIA Hong-Xiang, LIU Xiao-Kun, ZHAO Xiao-E, WEI Qiang, MA Bao-Hua (2012): Measuring the estrus cycle and its effect on superovulation in mice. Zoological Research, 33, 276-282  https://doi.org/10.3724/SP.J.1141.2012.03276
 
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