Expression of innate immunity genes in kuruma shrimp Marsupenaeus japonicus after in vivo stimulation with garlic extract (allicin)

https://doi.org/10.17221/7924-VETMEDCitation:Tanekhy M., Fall J. (2015): Expression of innate immunity genes in kuruma shrimp Marsupenaeus japonicus after in vivo stimulation with garlic extract (allicin). Veterinarni Medicina, 60: 39-47.
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In recent times, attention has focused on immunostimulants and plant products which could have beneficial effects in disease control. At present, the application of immunostimulants has been considered a more effective approach to health management in aquaculture through the enhancement of immune capability and disease resistance in shrimp. Garlic possesses bactericidal property against bacteria and can inhibit the growth of protozoa as well as gregarine infection in cultured shrimp. However, its effect on viral disease infection has not been well studied and requires further investigation. Garlic can also stimulate shrimp haemocytes as determined by cellular immune responses (phagocytic activity, superoxide anion production and phenoloxidase activity) suggesting that the immune-stimulatory properties of garlic will be useful for improvement of shrimp health. Here, we determined the expression of the immune-related genes Penaeidin, Crustin, Lysozyme, Toll-like, and tumour necrosis factor in kuruma shrimp, upon stimulation with allicin extract. The expression of these factors was measured for the first time and was found to be elevated in intestine and lymphoid organ after in vivo stimulation for 3, 12, 24 and 48 h. We conclude that garlic can be used in shrimp culture as an alternative to antibiotics or chemotherapeutic agents; however, further research is needed under field conditions.
References:
Amagase H, Petesch BL, Matsuura H, Kasuga S, Itakura Y (2001): Intake of garlic and its bioactive components. Journal of Nutrition 131, 955S–962S.
 
Ankri Serge, Mirelman David (1999): Antimicrobial properties of allicin from garlic. Microbes and Infection, 1, 125-129  https://doi.org/10.1016/S1286-4579(99)80003-3
 
Block Gladys, Patterson Blossom, Subar Amy (1992): Fruit, vegetables, and cancer prevention: A review of the epidemiological evidence. Nutrition and Cancer, 18, 1-29  https://doi.org/10.1080/01635589209514201
 
Burgents Joseph E., Burnett Karen G., Burnett Louis E. (2005): Effects of Hypoxia and Hypercapnic Hypoxia on the Localization and the Elimination of Vibrio campbellii in Litopenaeus vannamei, the Pacific White Shrimp. Biological Bulletin, 208, 159-  https://doi.org/10.2307/3593148
 
Cavallito Chester J., Buck Johannes S., Suter C. M. (1944): Allicin, the Antibacterial Principle of Allium sativum. II. Determination of the Chemical Structure. Journal of the American Chemical Society, 66, 1952-1954  https://doi.org/10.1021/ja01239a049
 
Chen SN, Huang SL, Kou GH (1992): Studies on the epizootiology and pathogenicity of bacterial infections in cultured giant tiger prawns, Penaeus monodon. Taiwan. In: Fulks W, Main KL (eds.): Disease of Cultured Penaeid Shrimp in Asia and the United State. The Oceanic Institute, Honolulu. 195–205.
 
Chutchawanchaipan W, Thavornyutikarn M, Kasornchandra J (2004): Application of using garlic paste against gregarines infestation in black tiger shrimp, Penaeus monodon. In: The 5th National Symposium on Marine Shrimp, Miracle Grand Convention, Bangkok, Thailand, 335–342.
 
Cowan MM (1999): Plant products as antimicrobial agents. Clinical and Microbial Reviews 12, 564–582.
 
Destoumieux D, Munoz M, Cosseau C, Rodriguez J, Bulet P, Comps M, Bachere E (2000): Penaeidins, antimicrobial peptides with chitin-binding activity, are produced and stored in shrimp granulocytes and released after microbial challenge. Journal of Cell Science 113, 461–469.
 
Dirsch Verena M, Kiemer Alexandra K, Wagner Hildebert, Vollmar Angelika M (1998): Effect of allicin and ajoene, two compounds of garlic, on inducible nitric oxide synthase. Atherosclerosis, 139, 333-339  https://doi.org/10.1016/S0021-9150(98)00094-X
 
Fall F, Kono T, Tanekhy M, Itami T, Sakai M (2010): Expression of innate immune-related genes of Kuruma shrimp, Marsupenaeus japonicus, after challenge with Vibrio nigripulchritudo. African Journal of Microbiology Research 4, 2426–2433.
 
Fu Yu-Win, Hou Wen-Ying, Yeh Su-Tuen, Li Chiu-Hsia, Chen Jiann-Chu (2007): The immunostimulatory effects of hot-water extract of Gelidium amansii via immersion, injection and dietary administrations on white shrimp Litopenaeus vannamei and its resistance against Vibrio alginolyticus. Fish & Shellfish Immunology, 22, 673-685  https://doi.org/10.1016/j.fsi.2006.08.014
 
Hou Wen-Ying, Chen Jiann-Chu (2005): The immunostimulatory effect of hot-water extract of Gracilaria tenuistipitata on the white shrimp Litopenaeus vannamei and its resistance against Vibrio alginolyticus. Fish & Shellfish Immunology, 19, 127-138  https://doi.org/10.1016/j.fsi.2004.11.009
 
Jayabalan N, Chandran R, Sivakumar V, Ramamoorthi K (1982): Occurrence of luminescent bacteria in sediment. Current Science 51, 710–711.
 
Ji Pei-Feng, Yao Cui-Luan, Wang Zhi-Yong (2009): Immune response and gene expression in shrimp (Litopenaeus vannamei) hemocytes and hepatopancreas against some pathogen-associated molecular patterns. Fish & Shellfish Immunology, 27, 563-570  https://doi.org/10.1016/j.fsi.2009.08.001
 
Jolles P, Jolles J (1984): What’s new in lysozyme research? Always a model system, today as yesterday. Molecular and Cellular Biochemistry 63, 165–189.
 
Kasornchandra J, Chutchawanchaipan W, Thavornyutikarn M, Puangkaew J (2005): Application of garlic (Allium sativum) as an alternate therapeutic for marine shrimp. In: Proceeding of the JSPS-NRCT International Symposium: Productivity techniques and effective utilization of aquatic animal resources into the new century. Kasetsart University, Thailand, 114–119.
 
Kono Tomoya, Sonoda Kohei, Kitao Yoichi, Mekata Tohru, Itami Toshiaki, Sakai Masahiro (2009): The Expression Analysis of Innate Immune-related Genes in Kuruma Shrimp Penaeus japonicus after DNA Vaccination against Penaeid Rod-shaped DNA Virus. Fish Pathology, 44, 94-97  https://doi.org/10.3147/jsfp.44.94
 
Young Lee So, Söderhäll Kenneth (2002): Early events in crustacean innate immunity. Fish & Shellfish Immunology, 12, 421-437  https://doi.org/10.1006/fsim.2002.0420
 
Lee Jeong-Yeol, Gao Yang (2012): Review of the Application of Garlic, Allium sativum, in Aquaculture. Journal of the World Aquaculture Society, 43, 447-458  https://doi.org/10.1111/j.1749-7345.2012.00581.x
 
Liao IC, Chang MS, Su CF, Her BY, Kojima T (1996): Enhancement of the resistance of grass prawn, Penaeus monodon against Vibrio damsel infection by beta-1,3-glucan. Journal of Fisheries Society Taiwan 23, 109–116.
 
Mekata Tohru, Kono Tomoya, Yoshida Terutoyo, Sakai Masahiro, Itami Toshiaki (2008): Identification of cDNA encoding Toll receptor, MjToll gene from kuruma shrimp, Marsupenaeus japonicus. Fish & Shellfish Immunology, 24, 122-133  https://doi.org/10.1016/j.fsi.2007.10.006
 
Mekata Tohru, Sudhakaran Raja, Okugawa Shogo, Inada Mari, Kono Tomoya, Sakai Masahiro, Itami Toshiaki (2010): A novel gene of tumor necrosis factor ligand superfamily from kuruma shrimp, Marsupenaeus japonicus. Fish & Shellfish Immunology, 28, 571-578  https://doi.org/10.1016/j.fsi.2009.12.020
 
Nya E J, Austin B (2009): Use of dietary ginger, Zingiber officinale Roscoe, as an immunostimulant to control Aeromonas hydrophila infections in rainbow trout, Oncorhynchus mykiss (Walbaum). Journal of Fish Diseases, 32, 971-977  https://doi.org/10.1111/j.1365-2761.2009.01101.x
 
Nya E J, Dawood Z, Austin B (2010): The garlic component, allicin, prevents disease caused by Aeromonas hydrophila in rainbow trout, Oncorhynchus mykiss (Walbaum). Journal of Fish Diseases, 33, 293-300  https://doi.org/10.1111/j.1365-2761.2009.01121.x
 
RATTANACHAI Achara, HIRONO Ikuo, OHIRA Tsuyoshi, TAKAHASHI Yukinori, AOKI Takashi (2004): Cloning of kuruma prawn Marsupenaeus japonicus crustin-like peptide cDNA and analysis of its expression. Fisheries Science, 70, 765-771  https://doi.org/10.1111/j.1444-2906.2004.00869.x
 
Song YL, Huang CC (1999): Application of immunostimulants to prevent shrimp diseases. In: Fingerman M, Nagabhushanam R (eds.): Immunobiology and Pathology. Recent Advances in Marine Biotechnology. Vol. 5. Enfield, Science Publishers, NH, USA. 173–188.
 
Sung H. H., Kou G. H., Song Y. L. (1994): Vibriosis Resistance Induced by Glucan Treatment in Tiger Shrimp (Penaeus monodon).. Fish Pathology, 29, 11-17  https://doi.org/10.3147/jsfp.29.11
 
Tanekhy M, Kono T, Sakai M (2010): Cloning, characterization, and expression analysis of Toll-like receptor-7 cDNA from common carp, Cyprinus carpio L. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics 5, 245–255.
 
Tanticharoen Morakot, Flegel Timothy W., Meerod Watcharin, Grudloyma Uthaiwan, Pisamai Nuchjaree (2008): Aquacultural biotechnology in Thailand: the case of the shrimp industry. International Journal of Biotechnology, 10, 588-  https://doi.org/10.1504/IJBT.2008.022494
 
van de Braak C.B.T., Botterblom M.H.A., Taverne N., van Muiswinkel W.B., Rombout J.H.W.M., van der Knaap W.P.W. (2002): The roles of haemocytes and the lymphoid organ in the clearance of injected Vibrio bacteria in Penaeus monodon shrimp. Fish & Shellfish Immunology, 13, 293-309  https://doi.org/10.1006/fsim.2002.0409
 
Wagner H. (1990): Search for plant derived natural products with immunostimulatory activity: recent advances. Pure and Applied Chemistry, 62, -  https://doi.org/10.1351/pac199062071217
 
Weber Norbert, Andersen Douglas, North James, Murray Byron, Lawson Larry, Hughes Bronwyn (1992): In Vitro Virucidal Effects of Allium sativum (Garlic) Extract and Compounds. Planta Medica, 58, 417-423  https://doi.org/10.1055/s-2006-961504
 
Lee Seong Wei, Najiah Musa (2009): Antimicrobial Property of 2-Hydroxypropane-1,2,3-Tricarboxylic Acid Isolated from Citrus microcarpa Extract. Agricultural Sciences in China, 8, 880-886  https://doi.org/10.1016/S1671-2927(08)60291-6
 
Woo SH, Lee JH, Kim YK, Chi MY, Jung SH, Kim JW, Park SI (2010): Effects of garlic Allium sativum extract immersion on the immune responses of olive flounder Paralichthys olivaceus prechallenged with pathogenic bacteria. Japan Society of Fish Pathology 23, 199–209.
 
Yoshida S, Kasuga S, Hayashi N, Ushiroguchi T, Matsuura H, Nakagawa S (1987): Antifungal activity of ajoene derived from garlic. Applied and Environmental Microbiology 53, 615–617.
 
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