Determination of exposure to Fasciola hepatica in horses from Uruguay using a recombinant-based ELISA
J. Sanchis, J. Suarez, GV Hillyer, JA Hernandez, MA Solari, C. Cazapal-Monteiro, AM Duque de Araujo, LM Madeira de Carvalho, A. Paz-Silva, R. Sanchez-Andrade, MS Ariashttps://doi.org/10.17221/8439-VETMEDCitation:Sanchis J., Suarez J., Hillyer G., Hernandez J., Solari M., Cazapal-Monteiro C., Duque de Araujo A., Madeira de Carvalho L., Paz-Silva A., Sanchez-Andrade R., Arias M. (2015): Determination of exposure to Fasciola hepatica in horses from Uruguay using a recombinant-based ELISA. Veterinarni Medicina, 60: 483-488.
The risk of exposure to Fasciola hepatica in horses from Uruguay was evaluated using ELISA and a recombinant surface protein (FhrAPS). Blood samples were collected from 368 horses from different districts. Detection of antibodies and the seasonal IgG pattern against the trematode was also established. The overall seroprevalence was 54% (ranging 23% November to 93% December). Significantly higher values were observed in the Anglo-Arab horses (86%) and in those older than xix year (63%). No sex- or origin-differences were observed. Two primary risk periods for infection in Uruguay have been identified, the end of spring and autumn. Exposure to the fluke should be taken into account among those horses feeding on pastures grazed by ruminants. Using FhrAPS-ELISA, helpful information concerning the risk of horses to the infection by trematode can be obtained. This test allows the opportunity to compare the results achieved in different laboratories by minimising specific regional effects.Keywords:Fasciola hepatica; equine; Uruguay; sensitisation; FhrAPSReferences:
Acici Mustafa, Bolukbas Cenk S., Gurler Ali T., Umur Sinasi, Buyuktanir Ozlem (2013): Seroprevalence of Fasciolosis in Equines of the Black Sea Region, Turkey. Journal of Equine Veterinary Science, 33, 62-66 https://doi.org/10.1016/j.jevs.2012.04.015Acosta D (1993): Epidemiology and control of F. hepatica in Uruguay (in Spanish). In: Nari A, Fiel C (eds.): Parasitic Diseases of Economic Importance in Cattle: Epidemiological Basis for Prevention and Control. Hemisferio Sur, Uruguay. 233–256.Alves RM, van Rensburg LJ, van Wyk JA (1988): Fasciola in horses in the Republic of South Africa: a single natural case of Fasciola hepatica and the failure to infest ten horses either with F. hepatica or Fasciola gigantica. Onderstepoort Journal of Veterinary Research 55, 157–163.Arias M., Morrondo P., Hillyer G.V., Sánchez-Andrade R., Suárez J.L., Lomba C., Pedreira J., Díaz P., Díez-Baños P., Paz-Silva A. (2007): Immunodiagnosis of current fasciolosis in sheep naturally exposed to Fasciola hepatica by using a 2.9kDa recombinant protein. Veterinary Parasitology, 146, 46-49 https://doi.org/10.1016/j.vetpar.2007.02.007Arias MS, Pineiro P, Hillyer GV, Francisco I, Cazapal-Monteiro CF, Suarez JL, Morrondo P, Sanchez-Andrade R, Paz-Silva A (2012a): Enzyme-linked immunosorbent assays for the detection of equine antibodies specific to a recombinant Fasciola hepatica surface antigen in an endemic area. Parasitology Research 110, 1001–1007.Arias MS, Martinez-Carrasco C, León-Vizcaino L, Paz-Silva A, Diez-Banos P, Morrondo P, Alonso F (2012b): Detection of antibodies in wild ruminants to evaluate exposure to liver trematodes. Journal of Parasitology 98, 754–759.Awad WS, Ibrahim AK, Salib FA (2009): Using indirect ELISA to assess different antigens for the serodiagnosis of Fasciola gigantica infection in cattle, sheep and donkeys, Research in Veterinary Science 86, 466–471.Castro O, Heinzen T, Carballo M (2001): Dynamics of Lymnaea viator infection with F. hepatica in natural conditions in Uruguay (in Spanish). Veterinaria Uruguay 36, 13–20.Eleftheratos K, Tselioudis G, Zerefos C, Nastos P, Douvis C, Kapsomenakis I (2011): Observed and predicted climate changes in Uruguay and adjacent areas. Journal of Geosciences 45, 83–90.Getachew M., Innocent G.T., Trawford A.F., Reid S.W.J., Love S. (2010): Epidemiological features of fasciolosis in working donkeys in Ethiopia. Veterinary Parasitology, 169, 335-339 https://doi.org/10.1016/j.vetpar.2010.01.007Grelck H, Horchner F, Wohrl H (1977): Experimental infection of horses with Fasciola hepatica. Berliner und Munchener Tierarztliche Wochenschrift 90, 371–373.Haridy FM, Morsy GH, Abdou NE, Morsy TA (2007): Zoonotic fascioliasis in donkeys: ELISA (Fges) and postmortum examination in the Zoo, Giza, Egypt. Journal of the Egyptian Society of Parasitology 37, 1101–1110.INIA-INAC, Instituto Nacional de. Investigacion Agropecuaria-Instituto Nacional de Carnes (2009): Second Audit of the Quality of the meat chain of beef from Uruguay (in Spanish). INAC, Uruguay. 46 pp.Marcos Luis A, Terashima Angelica, Gotuzzo Eduardo (2008): Update on hepatobiliary flukes: fascioliasis, opisthorchiasis and clonorchiasis. Current Opinion in Infectious Diseases, 21, 523-530 https://doi.org/10.1097/QCO.0b013e32830f9818Nansen P., Andersen S., Hesselholt M. (1975): Experimental infection of the horse with Fasciola hepatica. Experimental Parasitology, 37, 15-19 https://doi.org/10.1016/0014-4894(75)90049-1Nari A, Cardozo H, Solari MA, Petraccia C, Acosta D (1986): Preliminary study on the development of Limnaea viatrix D’Orbigny (1835) under controlled conditions of temperature and humidity. Veterinaria 22, 13–17.Paz-Silva A, Hillyer GV, Arias MS, Sanchez-Andrade R, Pedreira J, Suarez JL, Lomba C, Diaz P, Francisco I, Diez-Banos P, Morrondo P (2007): A cross-sectional study of fascioliasis in autochthonous cattle from NW Spain by using a 2.9-kDa recombinant protein. Journal of Applied Research in Veterinary Medicine 5, 52–56.Phiri I.K., Phiri A.M., Harrison L.J.S. (2006): Serum antibody isotype responses of Fasciola-infected sheep and cattle to excretory and secretory products of Fasciola species. Veterinary Parasitology, 141, 234-242 https://doi.org/10.1016/j.vetpar.2006.05.019Rojo-Vázquez F.A., Meana A., Valcárcel F., Martínez-Valladares M. (2012): Update on trematode infections in sheep. Veterinary Parasitology, 189, 15-38 https://doi.org/10.1016/j.vetpar.2012.03.029Sanchis J, Miguelez S, Solari MA, Pineiro P, Macchi MI, Maldini G, Venzal J, Morrondo P, Diez-Banos P, Sanchez-Andrade R, Paz-Silva A, Arias MS (2011): Seroprevalence of bovine fasciolosis in department Salto (Uruguay). Revista Ibero-Latinoamericana de Parasitologia 70, 163–171.Sanchis J, Hillyer G, Madeira de Carvalho LM, Macchi MI, Gomes C, Maldini G, Siltwell G, Venzal JM, Paz-Silva A, Sanchez-Andrade R, Arias MS (2014): Update on fasciolosis in extensively managed cattle from Uruguay and Portugal by using a recombinant protein-based ELISA. Archivos de Medicina Veterinaria 47, 201–208.Soule C, Boulard C, Levieux D, Barnouin J, Plateau E (1989): Experimental equine fascioliasis: evolution of serologic, enzymatic and parasitic parameters. Annals of Veterinary Research 20, 295–307.Soykan Emel, Oge Hatice (2012): The Prevalence of Liver Trematodes in Equines in Different Cities of Turkey. Turkish Journal of Parasitology, 36, 152-155 https://doi.org/10.5152/tpd.2012.36Thrusfield M (ed.) (2005): Veterinary Epidemiology. Blackwell Science Ltd, Oxford. 584 pp.