Levels of nitrogenous substances and amino acids in bodies of Ross 308 hybrid cocks and hens over the course of rearing
E. Strakova, P. Suchy, P. Navratil, I. Herzig, M. Machacekhttps://doi.org/10.17221/8441-VETMEDCitation:Strakova E., Suchy P., Navratil P., Herzig I., Machacek M. (2015): Levels of nitrogenous substances and amino acids in bodies of Ross 308 hybrid cocks and hens over the course of rearing. Veterinarni Medicina, 60: 499-508.
Over the course of Ross 308 hybrid broiler chick cock and hen rearing, whole chicken bodies including feathers were monitored for changes in the levels of nitrogenous substances, essential amino acids (EAA) and non-essential amino acids (NEAA). At five-day intervals (Days 1, 5, 9, 15, 20, 26, 30, 35 and 40 of rearing), randomly selected chickens were slaughtered after 24 h of fasting. Over the course of rearing, N-substance levels ranged from 629.1 to 429.0 g/kg dry matter in hen bodies and from 616.0 to 477.3 g/kg dry matter in cock bodies. N-substance levels were statistically significantly different in the two sexes on rearing Days 35 and 40 (P ≤ 0.01). The levels of all amino acids (AA) in dry body matter of chickens up to 15 days of age dropped significantly over time, while in the period from Day 20 to Day 40, some AA levels increased or reached the baseline. With most AA, the levels were lower in hens than in cocks over the course of the trial. At the end of the trial (Days 35 and 40), statistically significant differences between the sexes were found for most EAA (P ≤ 0.01; P ≤ 0.05). NEAA, except for Ser and Ala, were also significantly higher in cocks on Days 35 and 40 (P ≤ 0.01; P ≤ 0.05). Among the monitored EAA, the dry matter of the bodies of hens exhibited the highest levels of Leu, 8.70% on average, followed by Val 6.54%, Lys 5.26%, Ile 5.25%, Thr 4.84%, Phe 4.30%, Tyr 2.51% and Met 2.21%. Leu was also the most abundant in the cock bodies, 8.42% on average, followed by Val 6.30%, Lys 5.36%, Ile 5.06%, Thr 4.57%, Phe 4.45%, Tyr 2.88% and Met 2.17%. Knowledge of the levels of nitrogenous substances, EAA and NEAA in the whole bodies of broiler chickens including feathers will help to determine optimal rearing conditions.Keywords:broiler; nitrogenous substance; amino acid; essential; non-essentialReferences:
Anonymous (2007): Ross 308. Broiler Nutrition Specification. Accessed May 2014. http://en.aviagen.com/assets/Tech_Center/Ross_Broiler/Ross_308_Broiler_Nutrition_Spec.pdf.Anonymous (2012): Ross 308. Broiler Performance Objectives. Accessed May 2014. http://en.aviagen.com/assets/Tech_Center/Ross_Broiler/Ross308BroilerPerfObj2012R1.pdf.Aletor V?A, Hamid I?I, Nie� E, Pfeffer E (2000): Low-protein amino acid-supplemented diets in broiler chickens: effects on performance, carcass characteristics, whole-body composition and efficiencies of nutrient utilisation. Journal of the Science of Food and Agriculture, 80, 547-554 https://doi.org/10.1002/(SICI)1097-0010(200004)80:5<547::AID-JSFA531>3.0.CO;2-CAOAC, Association of Official Analytical Chemists (2003): Official Methods of Analysis. 23rd ed. International Association of Analytical Chemists, Washington, DC, USA.Applegate TJ, Angel R (2008): Protein and amino acid requirements for poultry. Animal Sciences. Accessed February 2015. http://puyallup.wsu.edu/dairy/nutrient-management/data/publications/Protein%20and%20amino%20acid%20for%20poultry-final.pdf.Baker DH (1997): Ideal amino acid profiles for swine and poultry and their application in feed formulation. Biokyowa Technical Review 9. Biokyowa, Chesterfield, MO.Bogosavljevic S, Pavlovski Z, Petrovic MD, Doskovic V, Rakonjac S (2010): Broiler meat quality: Proteins and lipids of muscle tissue. African Journal of Biotechnology 9, 9177–9182.Dozier W. A., Kidd M. T., Corzo A. (2008): Dietary Amino Acid Responses of Broiler Chickens. The Journal of Applied Poultry Research, 17, 157-167 https://doi.org/10.3382/japr.2007-00071Emmans GC (1989): Computer simulation in poultry nutrition. In: Proc. 3rd European Symposium Poultry Nutrition, Peebles, Scotland, 91–104.Hussein A. S., Cantor A. H., Pescatore A. J., Gates R. S., Burnham D., Ford M. J., Paton N. D. (2001): Effect of Low Protein Diets with Amino Acid Supplementation on Broiler Growth1. The Journal of Applied Poultry Research, 10, 354-362 https://doi.org/10.1093/japr/10.4.354Kreuzer M, Kirggessner M, Steinhart H (1988): Effect of the level of energy and protein supply, respectively of age on the amino acid composition of the broiler chicks. Archiv fur Geflugelkunde 52, 133–141.Meluzzi A, Sirri F, Castellini C, Roncarati A, Melotti P, Franchini A (2009): Influence of genotype and feeding on chemical composition of organic chicken meat. Italian Journal of Animal Science 8, 2S, 766–768.Mukhtar AM, Mekkawi A, El Tigani M (2007): The effect of feeding increasing levels of synthetic lysine and methionine in broiler chicks. Research Journal of Animal and Veterinary Sciences 2, 18–20.Nitsan Zafrira, Dvorin A., Nir I. (): Composition and amino acid content of carcass, skin and feathers of the growing gosling. British Poultry Science, 22, 79-84 https://doi.org/10.1080/00071688108447866NRC (1994): National Research Council. Nutrient Requirements of Poultry. 9th rev. ed. National Academies Press, Washington, DC. 173 pp.Pellett PL, Kaba H (1972): Carcass amino acids of the rat under condition of determination of net protein utilization. Journal of Nutrition 102, 61–68.Salehifar E., Shivazad M., Foroudi F., Chamani M., Bahari Kashani R. (2012): Reevaluation of digestible amino acid requirements of male and female broilers based on different ideal amino acids ratios in starter period. Livestock Science, 147, 154-158 https://doi.org/10.1016/j.livsci.2012.04.016Saunders AJ, Wessels JPH, Gous RM (1977): Carcass amino acid composition and utilization of dietary amino acids by chickens. South African Journal of Animal Science 7, 111–115.Sklan D., Noy Y. (2005): Direct determination of optimal amino acid intake for maintenance and growth in broilers. Poultry Science, 84, 412-418 https://doi.org/10.1093/ps/84.3.412Stilborn H. L., Moran E. T., Gous R. M., Harrison M. D. (1997): Effect of Age on Feather Amino Acid Content in Two Broiler Strain Crosses and Sexes. The Journal of Applied Poultry Research, 6, 205-209 https://doi.org/10.1093/japr/6.2.205Stilborn H. L., Moran E. T., Gous R. M., Harrison M. D. (2010): Influence of age on carcass (feather-free) amino acid content for two broiler strain-crosses and sexes. The Journal of Applied Poultry Research, 19, 13-23 https://doi.org/10.3382/japr.2009-00053Strakova E, Suchy P, Vitula F, Vecerek V (2006): Differences in the amino acid composition of muscles from pheasant and broiler chickens. Archive fur Tierzucht – Archives of Animal Breeding, Dummerstorf 49, 508–514.Suchy P, Jelinek P, Strakova E, Hucl J (2002): Chemical composition of muscles of hybrid broiler chickens during prolonged feeding. Czech Journal of Animal Science 47, 511–518.Zelenka J, Heger J, Zeman L (2007): Recommended Nutrient Content in Poultry Diets and Nutritive Value of Feeds for Poultry (in Czech). 1st ed. Mendel University of Agriculture and Forestry, Brno. 30 pp.