Sampling factors causing variability in milk constituents in early lactation cows

https://doi.org/10.17221/7920-VETMEDCitation:Cengiz M., Kaynar O., Cannazik O., Ileriturk M., Cengiz S., Hayirli A. (2015): Sampling factors causing variability in milk constituents in early lactation cows. Veterinarni Medicina, 60: 6-15.
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This study was conducted to evaluate effects of breed, parity, sampling day, and sampling time on somatic cell count (SCC) and milk lipid and protein profiles in intra-mammary infection-free early lactating dairy cows (Holstein, n = 5 and Swiss Brown, n = 6; 34 ± 6 DIM). SCC was assessed by flow cytometry. Milk lipid (free fatty acid, triacylglycerol, diacylglycerol, cholesterol, phospholipid) and protein IgG and A, albumin, caseins (α, β, κ), α-lactalbumin, and β-lactoglobulin were determined using HPTLC and SDS-PAGE methods. Data were analysed by 4-way ANOVA and subjected to hierarchical backward elimination of insignificant interaction terms. SCC varied by parity and sampling time. Apart from sampling time, other fixed factors altered milk lipids. There was only a breed × parity interaction effect on milk lipid profiles. All proposed factors affected milk proteins. Among proteins only the IgG fraction exhibited diurnal variation. There were significant breed × parity, breed × sampling day, and parity × sampling day interactions for milk proteins. In conclusion, factors relating to the cows themselves (breed and parity), sampling day, and sampling time cause variation in parameters that are pertinent to mammary gland health and milk quality.
References:
Auldist MJ, Coats S, Rogers GL, McDowell GH (1995): Changes in the composition of milk from healthy and mastitic dairy cows during the lactation cycle. Australian Journal of Experimental Agriculture, 35, 427-  https://doi.org/10.1071/EA9950427
 
Boscos C., Stefanakis A., Alexopoulos C., Samartzi F. (1996): Prevalence of subclinical mastitis and influence of breed, parity, stage of lactation and mammary bacteriological status on Coulter Counter Counts and California Mastitis Test in the milk of Saanen and autochthonous Greek goats. Small Ruminant Research, 21, 139-147  https://doi.org/10.1016/0921-4488(95)00824-1
 
Botaro BG, Gameiro AH, dos Santos MV (2013): Quality based payment program and milk quality in dairy cooperatives of Southern Brazil: an econometric analysis. Scientia Agricola 70, 21–26.
 
Cerbulis J., Farrell H.M. (1975): Composition of Milks of Dairy Cattle. I. Protein, Lactose, and Fat Contents and Distribution of Protein Fraction. Journal of Dairy Science, 58, 817-827  https://doi.org/10.3168/jds.S0022-0302(75)84644-3
 
DePeters E. J., Medrano J. F., Reed B. A. (1995): Fatty acid composition of milk fat from three breeds of dairy cattle. Canadian Journal of Animal Science, 75, 267-269  https://doi.org/10.4141/cjas95-040
 
Dohoo IR, Meek AH (1982): Somatic cell counts in bovine milk. Canadian Veterinary Journal 23, 119–125.
 
Forsbäck L., Lindmark-Månsson H., Andrén A., Åkerstedt M., Andrée L., Svennersten-Sjaunja K. (2010): Day-to-day variation in milk yield and milk composition at the udder-quarter level. Journal of Dairy Science, 93, 3569-3577  https://doi.org/10.3168/jds.2009-3015
 
Gaunt N. Stanley (1973): Genetic and Environmental Changes Possible in Milk Composition. Journal of Dairy Science, 56, 270-278  https://doi.org/10.3168/jds.S0022-0302(73)85162-8
 
Gilbert G.R., Hargrove G.L., Kroger M. (1973): Diurnal Variations in Milk Yield, Fat Yield, Milk Fat Percentage, and Milk Protein Percentage of Holstein-Friesian Cows. Journal of Dairy Science, 56, 409-410  https://doi.org/10.3168/jds.S0022-0302(73)85187-2
 
Gonzalo Carlos, Carriedo Juan A., Baro Jesus A., San Primitivo Fermin (1994): Factors Influencing Variation of Test Day Milk Yield, Somatic Cell Count, Fat, and Protein in Dairy Sheep. Journal of Dairy Science, 77, 1537-1542  https://doi.org/10.3168/jds.S0022-0302(94)77094-6
 
Hara Atsushi, Radin Norman S. (1978): Lipid extraction of tissues with a low-toxicity solvent. Analytical Biochemistry, 90, 420-426  https://doi.org/10.1016/0003-2697(78)90046-5
 
Harmon R.J. (1994): Physiology of Mastitis and Factors Affecting Somatic Cell Counts. Journal of Dairy Science, 77, 2103-2112  https://doi.org/10.3168/jds.S0022-0302(94)77153-8
 
Harmon RJ, Eberhart RJ, Jasper DE, Langlois BE, Wilson RA (1990): Microbiological procedures for the diagnosis of bovine udder infection. In: Proceedings of National Mastitis Council, Arlington (VA), USA, 4–34.
 
Heck J.M.L., van Valenberg H.J.F., Dijkstra J., van Hooijdonk A.C.M. (2009): Seasonal variation in the Dutch bovine raw milk composition. Journal of Dairy Science, 92, 4745-4755  https://doi.org/10.3168/jds.2009-2146
 
Jahreis G, Fritsch J, Steinhart H (1996): Monthly variations of milk composition with special regard to fatty acids depending on season and farm management systems-conventional versus ecological. European Journal of Lipid Science and Technology 98, 356–359.
 
Jensen RG (1995): Handbook of Milk composition. Access Online via Elsevier.
 
Kaartinen L (1995): Physiology of the bovine udder. In: Sandholm M, Honkanen-Buzalski T, Kaartinen L, Pyorala S (eds.): The Bovine Udder and Mastitis. 1st ed. University of Helsinki, Faculty of Veterinary Medicine. 14–23.
 
Kaynar Ozgur, Ileriturk Mustafa, Hayirli Armagan (2013): Evaluation of computational modifications in HPTLC with Gel analysis software and flatbed scanner for lipid separation. JPC - Journal of Planar Chromatography - Modern TLC, 26, 202-208  https://doi.org/10.1556/JPC.26.2013.3.1
 
Kehrli Marcus E., Shuster Dale E. (1994): Factors Affecting Milk Somatic Cells and Their Role in Health of the Bovine Mammary Gland. Journal of Dairy Science, 77, 619-627  https://doi.org/10.3168/jds.S0022-0302(94)76992-7
 
Kelsey J.A., Corl B.A., Collier R.J., Bauman D.E. (2003): The Effect of Breed, Parity, and Stage of Lactation on Conjugated Linoleic Acid (CLA) in Milk Fat from Dairy Cows. Journal of Dairy Science, 86, 2588-2597  https://doi.org/10.3168/jds.S0022-0302(03)73854-5
 
LAEMMLI U. K. (1970): Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature, 227, 680-685  https://doi.org/10.1038/227680a0
 
Laevens H., Deluyker H., Schukken Y.H., De Meulemeester L., Vandermeersch R., De Muêlenaere E., De Kruif A. (1997): Influence of Parity and Stage of Lactation on the Somatic Cell Count in Bacteriologically Negative Dairy Cows. Journal of Dairy Science, 80, 3219-3226  https://doi.org/10.3168/jds.S0022-0302(97)76295-7
 
Ma L (2012): Regulatory factors of milk fat synthesis in dairy cows. [PhD Dissertation.] Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
 
Maas J.A., France J., McBride B.W. (1997): Model of Milk Protein Synthesis. A Mechanistic Model of Milk Protein Synthesis in the Lactating Bovine Mammary Gland. Journal of Theoretical Biology, 187, 363-378  https://doi.org/10.1006/jtbi.1997.0437
 
Madureira Ana R., Pereira Cláudia I., Gomes Ana M.P., Pintado Manuela E., Xavier Malcata F. (2007): Bovine whey proteins – Overview on their main biological properties. Food Research International, 40, 1197-1211  https://doi.org/10.1016/j.foodres.2007.07.005
 
Mantere-Alhonen S (1995): Composition of milk. In: Sandholm M, Honkanen-Buzalski T, Kaartinen L, Pyorala S (eds.): The Bovine Udder and Mastitis. 1st ed. University of Helsinki, Faculty of Veterinary Medicine. 24–30.
 
Ng-Kwai-Hang K.F., Hayes J.F., Moxley J.E., Monardes H.G. (1984): Variability of Test-Day Milk Production and Composition and Relation of Somatic Cell Counts with Yield and Compositional Changes of Bovine Milk. Journal of Dairy Science, 67, 361-366  https://doi.org/10.3168/jds.S0022-0302(84)81309-0
 
Ng-Kwai-Hang K.F., Hayes J.F., Moxley J.E., Monardes H.G. (1987): Variation in Milk Protein Concentrations Associated with Genetic Polymorphism and Environmental Factors. Journal of Dairy Science, 70, 563-570  https://doi.org/10.3168/jds.S0022-0302(87)80042-5
 
Nielsen N.I., Larsen T., Bjerring M., Ingvartsen K.L. (2005): Quarter Health, Milking Interval, and Sampling Time During Milking Affect the Concentration of Milk Constituents. Journal of Dairy Science, 88, 3186-3200  https://doi.org/10.3168/jds.S0022-0302(05)73002-2
 
Palmquist D.L., Denise Beaulieu A., Barbano D.M. (1993): Feed and Animal Factors Influencing Milk Fat Composition. Journal of Dairy Science, 76, 1753-1771  https://doi.org/10.3168/jds.S0022-0302(93)77508-6
 
Quist M.A., LeBlanc S.J., Hand K.J., Lazenby D., Miglior F., Kelton D.F. (2008): Milking-to-Milking Variability for Milk Yield, Fat and Protein Percentage, and Somatic Cell Count. Journal of Dairy Science, 91, 3412-3423  https://doi.org/10.3168/jds.2007-0184
 
Renau JK (2001): Somatic cell counts: Measures of farm management and quality. In: Proceeding in Annual Meeting of National Mastitis Council, Nevada, USA, 29–37.
 
SAS (2002): User’s Guide. Statistics, Version 9. Statistical Analysis System. SAS Inst., Inc., Cary, NC, USA.
 
Schepers A.J., Lam T.J.G.M., Schukken Y.H., Wilmink J.B.M., Hanekamp W.J.A. (1997): Estimation of Variance Components for Somatic Cell Counts to Determine Thresholds for Uninfected Quarters. Journal of Dairy Science, 80, 1833-1840  https://doi.org/10.3168/jds.S0022-0302(97)76118-6
 
Schukken Ynte H., Wilson David J., Welcome Francis, Garrison-Tikofsky Linda, Gonzalez Ruben N. (2003): Monitoring udder health and milk quality using somatic cell counts. Veterinary Research, 34, 579-596  https://doi.org/10.1051/vetres:2003028
 
Schutz M.M., Hansen L.B., Steuernagel G.R., Kuck A.L. (1990): Variation of Milk, Fat, Protein, and Somatic Cells for Dairy Cattle. Journal of Dairy Science, 73, 484-493  https://doi.org/10.3168/jds.S0022-0302(90)78696-1
 
Sheldrake R.F., Hoare R.J.T., McGregor G.D. (1983): Lactation Stage, Parity, and Infection Affecting Somatic Cells, Electrical Conductivity, and Serum Albumin in Milk. Journal of Dairy Science, 66, 542-547  https://doi.org/10.3168/jds.S0022-0302(83)81823-2
 
Shields Shannon L, Rezamand Pedram, Sevier Dallace L, Seo Keun S, Price William, McGuire Mark A (2011): Effects of increased milking frequency for the first 21 days post partum on selected measures of mammary gland health, milk yield and milk composition. Journal of Dairy Research, 78, 301-307  https://doi.org/10.1017/S0022029911000410
 
Soyeurt H., Dardenne P., Gillon A., Croquet C., Vanderick S., Mayeres P., Bertozzi C., Gengler N. (2006): Variation in Fatty Acid Contents of Milk and Milk Fat Within and Across Breeds. Journal of Dairy Science, 89, 4858-4865  https://doi.org/10.3168/jds.S0022-0302(06)72534-6
 
Svennersten-Sjaunja K, Larsson U, Bertilsson J, Sjaunja LO (2005): The relative day-to-day variation in milk yield and composition for cows milked two or three times daily. In: Annual Meeting of the European Association for Animal Production, Uppsala, Sweden, 5–8 June, 382.
 
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