Changes in leukocyte counts, lymphocyte subpopulations and the mRNA expression of selected cytokines in the peripheral blood of dogs with atopic dermatitis Z., Svoboda M., Satinska D., Matiasovic J., Leva L., Toman M. (2015): Changes in leukocyte counts, lymphocyte subpopulations and the mRNA expression of selected cytokines in the peripheral blood of dogs with atopic dermatitis. Veterinarni Medicina, 60: 644-653.
download PDF
Canine atopic dermatitis (CAD) is a chronic allergic disease characterised by genetic predisposition. The exact pathogenesis of the disease is still not fully understood. Blood parameters, lymphocyte subpopulations, cytokines and other molecules involved in pro- and anti-inflammatory activities, and Th1/Th2 polarisation in peripheral blood were assessed in clinically healthy dogs and dogs with AD by haematological examination, flow cytometry and qRT-PCR. The group of diseased dogs was further divided into subgroups according to the severity of clinical signs expressed as CADESI-03 (canine atopic dermatitis extent and severity index) values. The group of dogs with CAD showed mild neutrophilia without general leucocytosis, reduced numbers of B-lymphocytes (P < 0.05), increased relative numbers of T-lymphocytes (P < 0.01) decreased absolute numbers of double-positive lymphocytes (P < 0.001) and NK cells (P < 0.05) as compared with healthy controls. The expression levels of IL-10 mRNA were significantly higher (P < 0.05) in the group with CAD. Correlation with CADESI-03 was observed in expression levels of CCL3 (P < 0.05), CCL5 (P < 0.05) and SOCS-3 mRNAs (P < 0.05). In conclusion, the disease stage investigated in the present study is characterised by a chronic inflammatory response.
Akdis C. A., Akdis M. (2003): Immunological differences between intrinsic and extrinsic types of atopic dermatitis. Clinical <html_ent glyph="@amp;" ascii="&amp;"/> Experimental Allergy, 33, 1618-1621
Akdis M. (2004): Immune Responses in Healthy and Allergic Individuals Are Characterized by a Fine Balance between Allergen-specific T Regulatory 1 and T Helper 2 Cells. Journal of Experimental Medicine, 199, 1567-1575
ARAKAWA S., HATANO Y., KATAGIRI K. (2004): Differential expression of mRNA for Th1 and Th2 cytokine-associated transcription factors and suppressors of cytokine signalling in peripheral blood mononuclear cells of patients with atopic dermatitis. Clinical and Experimental Immunology, 135, 505-510
Bismarck Doris, Schütze Nicole, Moore Peter, Büttner Mathias, Alber Gottfried, Buttlar Heiner v. (2012): Canine CD4+CD8+ double positive T cells in peripheral blood have features of activated T cells. Veterinary Immunology and Immunopathology, 149, 157-166
Bouloc Anne, Charue Dominique, Nikolova Maria, Bensussan Armand (2000): No Modulation of Circulating Natural Killer Cell and Natural Killer Receptor Bearing Memory T Cell Subsets in Patients with Atopic Dermatitis. Journal of Investigative Dermatology, 115, 1160-1162
de Vries I.Jolanda M., Langeveld-Wildschut Elisabeth G., van Reijsen Frank C., Dubois Gerald R., van den Hoek J.Annet, Bihari Ilse C., van Wichen Dick, de Weger Roel A., Knol Edward F., Thepen Theo, Bruijnzeel-Koomen Carla A.F.M. (1998): Adhesion molecule expression on skin endothelia in atopic dermatitis: Effects of TNF-α and IL-4. Journal of Allergy and Clinical Immunology, 102, 461-468
Dhabhar FS, Miller AH, McEwen BS, Spencer RL (1995): Effect of stress on immune cell distribution. Dynamics and hormonal mechanisms. Journal of Immunology 154, 5511–5527.
Duncan JR, Prasse KW, Mahaffey EA (1994): Haematology. In: Duncan JR, Prasse KW, Mahaffey EA (eds): Veterinary Laboratory Medicine: Clinical Pathology. Iowa State University Press, Iowa. 14–37.
Faldyna Martin, Levá Lenka, Knötigová Pavlı́na, Toman Miroslav (2001): Lymphocyte subsets in peripheral blood of dogs — a flow cytometric study. Veterinary Immunology and Immunopathology, 82, 23-37
Faldyna M, Toman M (1998): The effect of age on the distribution of lymphocyte and neutrophil granulocyte subsets in the peripheral blood of dog. Veterinarni Medicina 43, 193–199.
Favrot Claude, Steffan Jean, Seewald Wolfgang, Picco Federicca (2010): A prospective study on the clinical features of chronic canine atopic dermatitis and its diagnosis. Veterinary Dermatology, 21, 23-31
Gluck J, Rogala B (1999): Chemokine RANTES in atopic dermatitis. Archivum Immunologiae et Therapiae Exprimentalis 47, 367–372.
Griffin C.E, DeBoer D.J (2001): The ACVD task force on canine atopic dermatitis (XIV): clinical manifestations of canine atopic dermatitis. Veterinary Immunology and Immunopathology, 81, 255-269
Herbert T B, Cohen S (1993): Stress and immunity in humans: a meta-analytic review.. Psychosomatic Medicine, 55, 364-379
Hill Peter B, Olivry Thierry (2001): The ACVD task force on canine atopic dermatitis (V): biology and role of inflammatory cells in cutaneous allergic reactions. Veterinary Immunology and Immunopathology, 81, 187-198
Homey Bernhard, Steinhoff Martin, Ruzicka Thomas, Leung Donald Y.M. (2006): Cytokines and chemokines orchestrate atopic skin inflammation. Journal of Allergy and Clinical Immunology, 118, 178-189
Jackson H.A., Orton S.M., Hammerberg B. (2002): IgE is present on peripheral blood monocytes and B cells in normal dogs and dogs with atopic dermatitis but there is no correlation with serum IgE concentrations. Veterinary Immunology and Immunopathology, 85, 225-232
Kabelitz Dieter (2011): γδ T-cells: cross-talk between innate and adaptive immunity. Cellular and Molecular Life Sciences, 68, 2331-2333
Kaburagi Yuko, Shimada Yuka, Nagaoka Tetsuya, Hasegawa Minoru, Takehara Kazuhiko, Sato S. (2001): Enhanced production of CC-chemokines (RANTES, MCP-1, MIP-1&#x003B1;, MIP-1&#x003B2;, and eotaxin) in patients with atopic dermatitis. Archives of Dermatological Research, 293, 350-355
Kakinuma Takashi, Saeki Hidehisa, Tsunemi Yuichiro, Fujita Hideki, Asano Noriko, Mitsui Hiroshi, Tada Yayoi, Wakugawa Motoshi, Watanabe Takahiro, Torii Hideshi, Komine Mayumi, Asahina Akihiko, Nakamura Koichiro, Tamaki Kunihiko (2003): Increased serum cutaneous T cell-attracting chemokine (CCL27) levels in patients with atopic dermatitis and psoriasis vulgaris. Journal of Allergy and Clinical Immunology, 111, 592-597
Kashiwakura J.-i., Suzuki N., Nagafuchi H., Takeno M., Takeba Y., Shimoyama Y., Sakane T. (1999): Txk, a Nonreceptor Tyrosine Kinase of the Tec Family, Is Expressed in T Helper Type 1 Cells and Regulates Interferon   Production in Human T Lymphocytes. Journal of Experimental Medicine, 190, 1147-1154
Katsuta M., Takigawa Y., Kimishima M., Inaoka M., Takahashi R., Shiohara T. (): NK Cells and   + T Cells Are Phenotypically and Functionally Defective due to Preferential Apoptosis in Patients with Atopic Dermatitis. The Journal of Immunology, 176, 7736-7744
Keppel K.E., Campbell K.L., Zuckermann F.A., Greeley E.A., Schaeffer D.J., Husmann R.J. (2008): Quantitation of canine regulatory T cell populations, serum interleukin-10 and allergen-specific IgE concentrations in healthy control dogs and canine atopic dermatitis patients receiving allergen-specific immunotherapy. Veterinary Immunology and Immunopathology, 123, 337-344
Krebs DL, Hilton DJ (2000): SOCS: physiological suppressors of cytokine signaling. Journal of Cell Science 113, 2813–2819.
Kos Ferdynand J. (1998): Regulation of adaptive immunity by natural killer cells. Immunologic Research, 17, 303-312
Lahn M, Kalataradi H, Mittelstadt P, Pflum E, Vollmer M, Cady C, Mukasa A, Vella AT, Ikle D, Harbeck R, O’Brien R, Born W (1998): Early preferential stimulation of gamma delta T cells by TNF-alpha. Journal of Immunology 11, 5221–5230.
Lee Hyun-Jeong, Lee Ho-Pyo, Ha Seog-Jun, Byun Dae-Gyoo, Kim Jin-Wou (2000): Spontaneous expression of mRNA for IL-10, GM-CSF, TGF-β, TNF-α, and IL-6 in peripheral blood mononuclear cells from atopic dermatitis. Annals of Allergy, Asthma & Immunology, 84, 553-558
Machura E, Karczewska K, Mazur B, Jachimowicz M (2008): Serum levels of IL-2, IL-10, IL-13, IFNγ and TNFα in children with asthma and atopic dermatitis (AD). Allergy Asthma and Immunology 13, 172–181.
Maeda S., Ohmori K., Yasuda N., Kurata K., Sakaguchi M., Masuda K., Ohno K., Tsujimoto H. (2004): Increase of CC chemokine receptor 4-positive cells in the peripheral CD4+ cells in dogs with atopic dermatitis or experimentally sensitized to Japanese cedar pollen. Clinical <html_ent glyph="@amp;" ascii="&amp;"/> Experimental Allergy, 34, 1467-1473
Maeda Sadatoshi, Tsuchida Hiromi, Marsella Rosanna (2007): Allergen challenge decreases mRNA expression of regulatory cytokines in whole blood of high-IgE beagles. Veterinary Dermatology, 18, 422-426
Marsella Rosanna, Olivry Thierry (2003): Animal models of atopic dermatitis. Clinics in Dermatology, 21, 122-133
Moore Kevin W., de Waal Malefyt Rene, Coffman Robert L., O'Garra Anne (2001): I NTERLEUKIN -10 AND THE I NTERLEUKIN -10 R ECEPTOR. Annual Review of Immunology, 19, 683-765
Nuttall T.J, Knight P.A, McAleese S.M, Lamb J.R, Hill P.B (2002): T-helper 1, T-helper 2 and immunosuppressive cytokines in canine atopic dermatitis. Veterinary Immunology and Immunopathology, 87, 379-384
Olivry Thierry, Marsella Rosanna, Iwasaki Toshiroh, Mueller Ralf (2007): Validation of CADESI-03, a severity scale for clinical trials enrolling dogs with atopic dermatitis. Veterinary Dermatology, 18, 78-86
Olivry Thierry, Mueller Ralf, Nuttall Tim, Favrot Claude, Prélaud Pascal (2008): Determination of CADESI-03 thresholds for increasing severity levels of canine atopic dermatitis. Veterinary Dermatology, 19, 115-119
Olivry Thierry, Naydan Diane K., Moore Peter F. (1997): Characterization of the Cutaneous Inflammatory Infiltrate in Canine Atopic Dermatitis. The American Journal of Dermatopathology, 19, 477-486
Rot A. (1992): RANTES and macrophage inflammatory protein 1 alpha induce the migration and activation of normal human eosinophil granulocytes. Journal of Experimental Medicine, 176, 1489-1495
Scott DW, Miller WH, Griffin CE (2001): Skin immune system and allergic skin disease. In: Scott DW, Miller WH, Griffin CE (eds.): Muller and Kirk’s Small Animal Dermatology. 6th ed. W.B. Saunders, Philadelphia. 543–666.
Schlotter Yvette M., Rutten Victor P.M.G., Riemers Frank M., Knol Edward F., Willemse Ton (2011): Lesional skin in atopic dogs shows a mixed Type-1 and Type-2 immune responsiveness. Veterinary Immunology and Immunopathology, 143, 20-26
Schlotter Yvette M., Veenhof Eveline Z., Brinkhof Bas, Rutten Victor P.M.G., Spee Bart, Willemse Ton, Penning Louis C. (2009): A GeNorm algorithm-based selection of reference genes for quantitative real-time PCR in skin biopsies of healthy dogs and dogs with atopic dermatitis. Veterinary Immunology and Immunopathology, 129, 115-118
Sinke Jacqueline D., Thepen Theo, Bihari Ilse C., Rutten Victor P.M.G., Willemse Ton (1997): Immunophenotyping of skin-infiltrating T-cell subsets in dogs with atopic dermatitis. Veterinary Immunology and Immunopathology, 57, 13-23
Taszkun Iwona (2013): Expression of CD3, CD4, CD8, CD21, and MHC II lymphocyte antigens and serum IL-10 concentration in dogs with atopic dermatitis complicated by purulent dermatitis. Bulletin of the Veterinary Institute in Pulawy, 57, -
Taub D., Conlon K., Lloyd A., Oppenheim J., Kelvin D. (1993): Preferential migration of activated CD4+ and CD8+ T cells in response to MIP-1 alpha and MIP-1 beta. Science, 260, 355-358
THEPEN T, LANGEVELDWILDSCHUT E, BIHARI I, WICHEN D, REIJSEN F, MUDDE G, BRUIJNZEELKOOMEN C (1996): Biphasic response against aeroallergen in atopic dermatitis showing a switch from an initial TH2 response to a TH1 response in situ: An immunocytochemical study. Journal of Allergy and Clinical Immunology, 97, 828-837
Wehrmann W., Reinhold U., Kukel S., Franke N., Uerlich M., Kreysel H.W. (1990): Selective Alterations in Natural Killer Cell Subsets in Patients with Atopic Dermatitis. International Archives of Allergy and Immunology, 92, 318-322
Werfel T., Kapp A. (1998): Environmental and other major provocation factors in atopic dermatitis. Allergy, 53, 731-739
Zelnickova Petra, Matiasovic Jan, Pavlova Barbora, Kudlackova Hana, Kovaru Frantisek, Faldyna Martin (2008): Quantitative nitric oxide production by rat, bovine and porcine macrophages. Nitric Oxide, 19, 36-41
Zheng Wei-ping, Flavell Richard A (1997): The Transcription Factor GATA-3 Is Necessary and Sufficient for Th2 Cytokine Gene Expression in CD4 T Cells. Cell, 89, 587-596
Zlotnik Albert, Yoshie Osamu (2000): Chemokines. Immunity, 12, 121-127
download PDF

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti