Concentrations of serum amyloid A, haptoglobin, tumour necrosis factor and interleukin-1 and -6 in Anatolian buffaloes naturally infected with dermatophytosis-

https://doi.org/10.17221/8770-VETMEDCitation:Kabu M., Sayin Z. (2016): Concentrations of serum amyloid A, haptoglobin, tumour necrosis factor and interleukin-1 and -6 in Anatolian buffaloes naturally infected with dermatophytosis-. Veterinarni Medicina, 61: 133-135.
download PDF
Dermatophytosis is most frequently found in ruminants, in which non-pruritic periocular lesions are most typical, though generalised skin disease may develop. Accordingly, the infection causes major economic losses. The aim of this study was to measure the inflammatory status of Anatolian buffaloes with dermatophytosis by determining the serum concentrations of serum amyloid A (SAA), haptoglobin (Hp), tumour necrosis factor (TNF-α) and interleukin-1 (IL-1) and -6 (IL-6). Anatolian buffaloes (n = 26), aged three to 11 month, were divided into two groups: 11 animals served as the clinically healthy control group and 15 animals clinically and microbiologically diagnosed with dermatophytosis formed the experimental group. Concentrations of tested proteins were measured using commercially available ELISA kits. In all cases, concentrations of measured proteins were significantly higher (P < 0.05) in infected animals when compared to healthy controls: SAA: 41.05 ± 0.01 vs. 7.43 ± 0.11µg/ml; Hp: 96.21 ± 0.18 vs. 8.49 ± 0.79 µg/ml; TNF-α: 0.90 ± 0.99 vs. 0.10 ± 0.26 ng/ml; IL-1α: 186.22 ± 0.22 vs. 74.04 ± 0.90 pg/ml; and IL-6: 55.94 ± 0.50 vs. 32.45 ± 0.20 pg/ml. It was concluded that the elevated values of variables under study were a result of the inflammatory response to dermatophytosis; thus, these markers may serve as an additional diagnostic tool.
References:
Aslan O, Aksoy A, Ica T (2010): Serum concentrations of zinc, copper, manganese in young cattle with dermatophytosis. Journal of Faculty of Veterinary Medicine, Erciyes University 7, 29–33.
 
Ceciliani F., Ceron J.J., Eckersall P.D., Sauerwein H. (2012): Acute phase proteins in ruminants. Journal of Proteomics, 75, 4207-4231  https://doi.org/10.1016/j.jprot.2012.04.004
 
Eckersall P.D., Bell R. (2010): Acute phase proteins: Biomarkers of infection and inflammation in veterinary medicine. The Veterinary Journal, 185, 23-27  https://doi.org/10.1016/j.tvjl.2010.04.009
 
El-Bahr (2013): ACUTE PHASE PROTEINS, LIPID PROFILE AND PROINFLAMMATORY CYTOKINES IN HEALTHY AND BRONCHOPNEUMONIC WATER BUFFALO CALVES. American Journal of Biochemistry and Biotechnology, 9, 34-40  https://doi.org/10.3844/ajbbsp.2013.34.40
 
Gonzalez F. H. D., Tecles F., Martinez-Subiela S., Tvarijonaviciute A., Soler L., Ceron J. J. (2008): Acute Phase Protein Response in Goats. Journal of Veterinary Diagnostic Investigation, 20, 580-584  https://doi.org/10.1177/104063870802000507
 
Gudding R, Lund A (1995): Immunoprophylaxis of bovine dermatophytosis. The Canadian Veterinary Journal 36, 302–306.
 
Koneman EW, Roberts GD (1985): Practical Laboratory Mycology. The Williams and Wilkins Co., Baltimore.
 
Kumar Vijay, Kumar Parveen, Mohan Krishna, Sarkar Mihir, Suresh K. P., Chauhan M. S., Prakash B. S. (2011): Temporal changes in circulating levels of plasma interleukin-8 during peripartum period in Murrah buffaloes (Bubalus bubalis) under subtropical climate. Tropical Animal Health and Production, 43, 669-674  https://doi.org/10.1007/s11250-010-9751-7
 
Molina EC (2005): Serum interferon-gamma and interleukins-6 and -8 during infection with Fasciola gigantica in cattle and buffaloes. Journal of Veterinary Science 6, 135–139.
 
Murata H., Shimada N., Yoshioka M. (2004): Current research on acute phase proteins in veterinary diagnosis: an overview. The Veterinary Journal, 168, 28-40  https://doi.org/10.1016/S1090-0233(03)00119-9
 
Ozkanlar YE, Aktas MS, Kirecci, E (2009): Mycozoonozis associated with ringworm of calves in Erzurum Province Turkey. Kafkas Journal of the Faculty of Veterinary Medicine 15, 141–144.
 
Papini R., Nardoni S., Fanelli A., Mancianti F. (2009): High Infection Rate of Trichophyton verrucosum in Calves from Central Italy. Zoonoses and Public Health, 56, 59-64  https://doi.org/10.1111/j.1863-2378.2008.01157.x
 
Scott DB, Coetzer JAW, Thomson GR, Tustin RC (eds.) (1994): Mycoses. Infectious diseases of livestock with special reference to South Africa. Vol 2. Oxford University Press, Cape Town. 1521–1533.
 
Tothova C, Nagy O, Kovac G (2014): Acute phase proteins and their use in the diagnosis of diseases in ruminants. Veterinarni Medicina 59, 163–180.
 
Yilmazer RE, Aslan O (2010): The investigation of efficacy of Neguvon-Whitfield’s ointment combinations for the treatment of ringworm in cattle. Journal of Health Sciences 19, 175–183. 
 
download PDF

© 2020 Czech Academy of Agricultural Sciences