Coxiella burnetii in samples from cattle herds and sheep flocks in the Kars region of Turkey
A. Gulmez Saglam, M. Sahinhttps://doi.org/10.17221/8678-VETMEDCitation:Gulmez Saglam A., Sahin M. (2016): Coxiella burnetii in samples from cattle herds and sheep flocks in the Kars region of Turkey. Veterinarni Medicina, 61: 17-22.
This study was aimed at determining the presence of C. burnetii in cattle and sheep herds in the Kars region of Turkey using serological and molecular methods. As a serological technique, C.burnetii IgG in blood sera and milk samples were investigated with ELISA. The results of these examinations revealed that 108 (43.2%) out of 250 sheep blood serum samples and 52 (14.85%) out of 350 cattle blood serum samples were seropositive for C. burnetii antibodies by ELISA. Out of 350 cattle and 250 sheep milk samples examined with ELISA, 36 (10.28%) and 42 (16.8%) were found to be seropositive, respectively. For molecular analysis, a Trans-PCR amplifying the IS1111A transposase gene of C. burnetii was conducted. Five (1.42%) out of 350 cattle milk samples, and one (0.4%) from 250 sheep milk samples were found to be positive for C. burnetii DNA. The results obtained in this study have demonstrated the presence of Q fever in cattle and sheep in the Kars region, and the dissemination of the infectious agent within milk. This situation poses a potential risk for animal and human health. Ultimately, this study points the way to future investigations into the presence of C. burnetii, which causes Q fever in cattle and sheep, and will contribute to the protection and control of the disease.Keywords:Coxiella burnetii; Q fever; cattle; sheep; sera; ELISA; PCRReferences:
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