Coxiella burnetii in samples from cattle herds and sheep flocks in the Kars region of Turkey

https://doi.org/10.17221/8678-VETMEDCitation:Gulmez Saglam A., Sahin M. (2016): Coxiella burnetii in samples from cattle herds and sheep flocks in the Kars region of Turkey. Veterinarni Medicina, 61: 17-22.
download PDF
This study was aimed at determining the presence of C. burnetii in cattle and sheep herds in the Kars region of Turkey using serological and molecular methods. As a serological technique, C.burnetii IgG in blood sera and milk samples were investigated with ELISA. The results of these examinations revealed that 108 (43.2%) out of 250 sheep blood serum samples and 52 (14.85%) out of 350 cattle blood serum samples were seropositive for C. burnetii antibodies by ELISA. Out of 350 cattle and 250 sheep milk samples examined with ELISA, 36 (10.28%) and 42 (16.8%) were found to be seropositive, respectively. For molecular analysis, a Trans-PCR amplifying the IS1111A transposase gene of C. burnetii was conducted. Five (1.42%) out of 350 cattle milk samples, and one (0.4%) from 250 sheep milk samples were found to be positive for C. burnetii DNA. The results obtained in this study have demonstrated the presence of Q fever in cattle and sheep in the Kars region, and the dissemination of the infectious agent within milk. This situation poses a potential risk for animal and human health. Ultimately, this study points the way to future investigations into the presence of C. burnetii, which causes Q fever in cattle and sheep, and will contribute to the protection and control of the disease.
References:
Agger Jens F, Christoffersen Anna-Bodil, Rattenborg Erik, Nielsen Jørgen, Agerholm Jørgen S (2010): Prevalence of Coxiella burnetii antibodies in Danish dairy herds. Acta Veterinaria Scandinavica, 52, 5-  https://doi.org/10.1186/1751-0147-52-5
 
Aitken I. D. (1989): Clinical aspects and prevention of Q fever in animals. European Journal of Epidemiology, 5, 420-424  https://doi.org/10.1007/BF00140132
 
Angelakis Emmanouil, Raoult Didier (2010): Q fever. Veterinary Microbiology, 140, 297-309  https://doi.org/10.1016/j.vetmic.2009.07.016
 
Arricau-Bouvery N, Rodolakis A (2005): Is Q Fever an emerging or re-emerging zoonosis? Veterinary Research 36, 327–349.
 
Arserim NB, Yesilmen S, Tel OY, Ozekinci T, Keskin O, Pulat H, Vural A (2011): Seroprevalance of Coxiellosis in cows, sheep, goats and humans in Diyarbakir region of Turkey. African Journal of Microbiology Research 5, 2041–2043.
 
Berri Mustapha, Laroucau Karine, Rodolakis Annie (2000): The detection of Coxiella burnetii from ovine genital swabs, milk and fecal samples by the use of a single touchdown polymerase chain reaction. Veterinary Microbiology, 72, 285-293  https://doi.org/10.1016/S0378-1135(99)00178-9
 
Berri M., Souriau A., Crosby M., Crochet D., Lechopier P., Rodolakis A. (2001): Relationships between the shedding of Coxiella burnetii, clinical signs and serological responses of 34 sheep. Veterinary Record, 148, 502-505  https://doi.org/10.1136/vr.148.16.502
 
Berri Mustapha, Souriau Armel, Crosby Milton, Rodolakis Annie (2002): Shedding of Coxiella burnetii in ewes in two pregnancies following an episode of Coxiella abortion in a sheep flock. Veterinary Microbiology, 85, 55-60  https://doi.org/10.1016/S0378-1135(01)00480-1
 
Berri Mustapha, Rekiki Abdessalem, Boumedine Karim, Rodolakis Annie (2009): Simultaneous differential detection of Chlamydophila abortus, Chlamydophila pecorum and Coxiella burnetii from aborted ruminant's clinical samples using multiplex PCR. BMC Microbiology, 9, 130-  https://doi.org/10.1186/1471-2180-9-130
 
Brennan R. E., Samuel J. E. (2003): Evaluation of Coxiella burnetii Antibiotic Susceptibilities by Real-Time PCR Assay. Journal of Clinical Microbiology, 41, 1869-1874  https://doi.org/10.1128/JCM.41.5.1869-1874.2003
 
Cantas Hasan, Muwonge Adrian, Sareyyupoglu Baris, Yardimci Hakan, Skjerve Eystein (2011): Q fever abortions in ruminants and associated on-farm risk factors in northern Cyprus. BMC Veterinary Research, 7, 13-  https://doi.org/10.1186/1746-6148-7-13
 
Drancourt M, Raoult D (2005): Genus I. Coxiella. In: Brenner DJ, Krieg NR, Staley JT (eds.): Bergey’s Manual of Systematic Bacteriology, Vol. 2. The Gammaproteobacteria. 2nd ed. Springer-Verlag, New York. 237–242.
 
Fournier PE, Marrie TJ, Raoult D (1998): Diagnosis of Q fever. Journal of Clinical Microbiology 36, 1823–1834.
 
Guatteo R, Beaudeau F, Joly A, Seegers H (2007): Performances of an ELISA applied to serum and milk for the detection of antibodies to Coxiella burnetii in dairy cattle. Revue de Medecine Veterinaire 158, 250–252.
 
Guatteo R., Joly A., Beaudeau F. (2012): Shedding and serological patterns of dairy cows following abortions associated with Coxiella burnetii DNA detection. Veterinary Microbiology, 155, 430-433  https://doi.org/10.1016/j.vetmic.2011.09.026
 
Hartzell Joshua D., Wood-Morris Robert N., Martinez Luis J., Trotta Richard F. (2008): Q Fever: Epidemiology, Diagnosis, and Treatment. Mayo Clinic Proceedings, 83, 574-579  https://doi.org/10.1016/S0025-6196(11)60733-7
 
Hoover T A, Vodkin M H, Williams J C (1992): A Coxiella burnetti repeated DNA element resembling a bacterial insertion sequence.. Journal of Bacteriology, 174, 5540-5548  https://doi.org/10.1128/jb.174.17.5540-5548.1992
 
Kalender H (2001): The incidence of Coxiella burnetii infection in ewes in Elazig and neighboring provinces. Turkish Journal of Veterinary and Animal Sciences 25, 51–55.
 
Karaca M, Akkan HA, Cetin Y, Keles I, Tutuncu M, Ozkan C, Tasal I (2009): Studies on the determination of seroprevalance of Q fever in sheep in the region of Van. Journal of Animal and Veterinary Advances 8, 1925–1928.
 
Kargar Mohammad, Rashidi Afsaneh, Doosti Abbas, Ghorbani-Dalini Sadegh, Najafi Akram (2013): Prevalence of Coxiella burnetii in bovine bulk milk samples in southern Iran. Comparative Clinical Pathology, 22, 331-334  https://doi.org/10.1007/s00580-012-1406-9
 
Khalili M, Sakhaee E (2009): An update on a serologic survey of Q fever in domestic animal in Iran. American Journal of Tropical Medicine and Hygiene 80, 1031–1032.
 
Kilic S, Celebi B (2008): Part I: General information. Turk Hijyen ve Deneysel Biyoloji Dergisi 65, 3.
 
Kirkan S, Kaya O, Tekbiyik S, Parin U (2008): Detection of Coxiella burnetii in cattle by PCR. Turkish Journal of Veterinary and Animal Sciences 32, 215–220.
 
Klee SR, Tyczka J, Ellerbrok H, Franz T, Linke S, Baljer G, Appel B (2006): Highly sensitive real-time PCR for specific detection and quantification of Coxiella burnetii. BMC Microbiology 6, 2. DOI: 10.1186/1471-2180-6-2 https://doi.org/10.1186/1471-2180-6-2
 
Kovacova E, Kazar J (2000): Rickettsial diseases and their serological diagnosis. Clinical Laboratory 46, 239–245.
 
Kovacova E, Kazar J (2002): Q fever-still a query and underestimated infectious disease. Acta Virologica 4, 193–210.
 
Leloglu N (1977): Studies on Q fever in human, sheep and cattle in Erzurum, Kars and Agri provinces. Ataturk Universitesi Ziraat Fakultesi Dergisi 8, 113–131.
 
Masala Giovanna, Porcu Rosaura, Sanna Giovanna, Chessa Giovanna, Cillara Grazia, Chisu Valentina, Tola Sebastiana (2004): Occurrence, distribution, and role in abortion of Coxiella burnetii in sheep and goats in Sardinia, Italy. Veterinary Microbiology, 99, 301-305  https://doi.org/10.1016/j.vetmic.2004.01.006
 
Maurin M, Raoult D (1999): Q Fever. Clinical Microbiology Reviews 12, 518–553.
 
Ozgur NY, Hasoksuz M, Yilmaz H, Ikiz S, Ilgaz A (1997): Detection of antibodies to Coxiella burnetii in cattle with infertility problems and human sera by ELISA and its seroprevalence. Pendik Vet. Mikrob. Derg 2, 207–218.
 
Raoult D, Mege JL, Marrie T (2001): Q Fever: Queries remaining after decades of research. In: Scheld WM, Craig WA, Hughes JM (eds.): Emerging Infections 5. 1st ed. AS Press, Washington, DC. 29–56.
 
Seyitoglu F, Ozkurt Z, Okumus B (2006): The seroprevalance of Coxiellosis in Farmers and cattle in Erzurum District in Turkey. Turkish Journal of Veterinary and Animal Sciences 30, 71–75.
 
Thompson HA, Dennis DT, Dasch GA (2005): Q fever. In: Goodman (eds.): Tick-Borne Diseases of Humans. 1st ed. ASM Press, Washington, DC. 328–343.
 
Ho To, Htwe Khin Khin, Yamasaki Naomi, Zhang Guo Quan, Ogawa Motohiko, Yamaguchi Tsuyoshi, Fukushi Hideto, Hirai Katsuya (1995): Isolation of Coxiella burnetii from Dairy Cattle and Ticks, and Some Characteristics of the Isolates in Japan. Microbiology and Immunology, 39, 663-671  https://doi.org/10.1111/j.1348-0421.1995.tb03254.x
 
download PDF

© 2020 Czech Academy of Agricultural Sciences