Therapeutic effects of a combined antibiotic-enzyme treatment on subclinical mastitis in lactating dairy cows
B. Khoramian, M. Emaneini, M. Bolourchi, A. Niasari-Naslaji, A. Gorganzadeh, S. Abani, P. Hovareshtihttps://doi.org/10.17221/8876-VETMEDCitation:Khoramian B., Emaneini M., Bolourchi M., Niasari-Naslaji A., Gorganzadeh A., Abani S., Hovareshti P. (2016): Therapeutic effects of a combined antibiotic-enzyme treatment on subclinical mastitis in lactating dairy cows. Veterinarni Medicina, 61: 237-242.
ABSTRACT: The objective of this study was to evaluate a combined antibiotic-enzyme therapy for Staphylococcus aureus mastitis and biofilm formation. A total of 141 cases of S. aureus chronic mastitis from three farms were divided into two groups: the control group (n = 54) were treated with Nafpenzal® ointment; the enzyme + antibiotic group (n = 87) were treated with Nafpenzal® plus an enzymatic ointment (MastiVeyxym®). Quantitative determination of biofilm formation was determined using a colorimetric microplate assay and the detection of ica genes by PCR. Enzyme + antibiotic therapy did not significantly improve cure rates compared to control (48.3 vs 38.9%). The cure rate for infections caused by biofilm-positive strains was 42.6 and 46.6% for control and enzyme + antibiotic groups, respectively (P > 0.05). Comparison of cure rates between farms showed a relationship with somatic cell count (SCC), parity and oxacillin resistance. 79.4% of the isolates produced biofilm and antibacterial resistance rates for oxacillin, penicillin and streptomycin were 25.5, 71.6 and 95.7%, respectively. These results indicate that while the increase in mastitis cure rate using enzymatic therapy was not significant, this treatment could be useful in some situations.Keywords:
mastitis; Staphylococcus aureus; biofilm; enzyme-antibiotic treatment; genomic DNA; ica genesReferences:
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