Effects of dietary Enterococcus faecium on growth performance, carcass characteristics, faecal microbiota, and blood profile in broilers

https://doi.org/10.17221/8680-VETMEDCitation:Mohammadi Gheisar M., Hosseindoust A., Kim I.H. (2016): Effects of dietary Enterococcus faecium on growth performance, carcass characteristics, faecal microbiota, and blood profile in broilers. Veterinarni Medicina, 61: 28-34.
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This research was performed to evaluate the effect of supplementing broiler diets with a probiotic containing Enterococcus faecium on growth performance, carcass characteristics, faecal microbiota, and blood profile. A total of 384 one-day-old Ross 308 broiler chicks (mixed gender) with an average initial BW of 39.2 g were used in a 35 days feeding trial. The chicks were allotted to pens with 16 birds per pen and eight replications per treatment with food and water provided ad libitum. Treatments were: (1) basal diet, (2) 0.25% probiotic, and (3) 0.5% probiotic. Results indicated that body weight gain (BWG) on Day 7 to 21, Day 21 to 35 and overall (0 to 35) increased (P < 0.05) linearly but feed intake (FI) and feed conversion ratio (FCR) were not affected. A linear increase (P < 0.05) was observed in the relative weight of breast muscle when comparing the 0 to 0.5% concentration of probiotic, but breast meat colour was not affected by treatments. A significant impact (linear effect, P < 0.05) was observed on drip loss on Day 1. Inclusion of probiotic decreased (P < 0.05) the count of Salmonella linearly but the counts of E. coli and Lactobacillus were not affected. There was no remarkable influence on blood profile. Thus, it was concluded that inclusion of a probiotic containing Enterococcus faecium improved growth performance and altered the intestinal microbial population, without any negative effects on meat colour and blood profile in broiler chickens.
broiler; carcass characteristics; Enterococcus faecium; faecal microbiota
Anjum MI, Khan AG, Azim A, Afzal M (2005): Effect of dietary supplementation of multi-strain probiotic on broiler growth performance. Pakistan Veterinary Journal 25, 25–29.
Chen Y. J., Son K. S., Min B. J., Cho J. H., Kwon O. S., Kim I. H. (2005): Effects of Dietary Probiotic on Growth Performance, Nutrients Digestibility, Blood Characteristics and Fecal Noxious Gas Content in Growing Pigs. Asian-Australasian Journal of Animal Sciences, 18, 1464-1468  https://doi.org/10.5713/ajas.2005.1464
Depta A, Rychlik R, Nieradka R, Rotkiewicz T, Kujawa K, Bomba A, Grabowska-Swiecicka G (1998): The influence of alimentary tract colonization with Lactobacillus sp. strains on chosen metabolic profile indices in piglets. Polish Journal of Veterinary Science 1, 3–7.
Fairchild A. S., Grimes J. L., Jones F. T., Wineland M. J., Edens F. W., Sefton A. E. (2001): Effects of Hen Age, Bio-Mos,(R) and Flavomycin(R) on Poult Susceptibility to Oral Escherichia coli Challenge. Poultry Science, 80, 562-571  https://doi.org/10.1093/ps/80.5.562
Fooks L. J., Gibson G. R. (2002): Probiotics as modulators of the gut flora. British Journal of Nutrition, 88, s39-  https://doi.org/10.1079/BJN2002628
Fuller Roy (2001): The Chicken Gut Microflora and Probiotic Supplements. The Journal of Poultry Science, 38, 189-196  https://doi.org/10.2141/jpsa.38.189
Higgins S. E., Higgins J. P., Wolfenden A. D., Henderson S. N., Torres-Rodriguez A., Tellez G., Hargis B. (2008): Evaluation of a Lactobacillus-Based Probiotic Culture for the Reduction of Salmonella Enteritidis in Neonatal Broiler Chicks. Poultry Science, 87, 27-31  https://doi.org/10.3382/ps.2007-00210
Honikel Karl O. (1998): Reference methods for the assessment of physical characteristics of meat. Meat Science, 49, 447-457  https://doi.org/10.1016/S0309-1740(98)00034-5
Hooge D. M., Ishimaru H., Sims M. D. (2004): Influence of Dietary Bacillus subtilis C-3102 Spores on Live Performance of Broiler Chickens in Four Controlled Pen Trials1. The Journal of Applied Poultry Research, 13, 222-228  https://doi.org/10.1093/japr/13.2.222
Huang MK, Choi JL, Houde R, Lee JW, Lee B, Zhao X (2004): Effects of lactobacilli and an acidophilic fungus on the production in Nigeria. World Journal of Microbiology and Biotechnology 20, 51–56.
Jadamus A, Vahjen W, Simon O (2001): Growth behavior of a spore forming probiotic strain in the gastrointestinal tract of broiler chicken and piglets. Archives of Animal Breeding 54, 1–17.
. S.M.L. Kabir, . M.M. Rahman, . M.B. Rahman, . M.M. Rahman, . S.U. Ahmed (2004): The Dynamics of Probiotics on Growth Performance and Immune Response in Broilers. International Journal of Poultry Science, 3, 361-364  https://doi.org/10.3923/ijps.2004.361.364
Lodemann Ulrike, Hübener Katrin, Jansen Nicole, Martens Holger (2006): Effects of Enterococcus faecium NCIMB 10415 as probiotic supplement on intestinal transport and barrier function of piglets. Archives of Animal Nutrition, 60, 35-48  https://doi.org/10.1080/17450390500468099
Lodemann Ulrike, Lorenz Barbara Martha, Weyrauch Karl Dietrich, Martens Holger (2008): Effects of Bacillus cereus var. toyoi as probiotic feed supplement on intestinal transport and barrier function in piglets. Archives of Animal Nutrition, 62, 87-106  https://doi.org/10.1080/17450390801912068
Markovic R, Sefer D, Krstic M, Petrujkic B (2009): Effect of different growth promoters on broiler performance and gut morphology. Archivos de Medicina Veterinaria 41, 163–169.
Mathew A G, Chattin S E, Robbins C M, Golden D A (1998): Effects of a direct-fed yeast culture on enteric microbial populations, fermentation acids, and performance of weanling pigs.. Journal of Animal Science, 76, 2138-  https://doi.org/10.2527/1998.7682138x
Meng Q. W., Yan L., Ao X., Zhou T. X., Wang J. P., Lee J. H., Kim I. H. (): Influence of probiotics in different energy and nutrient density diets on growth performance, nutrient digestibility, meat quality, and blood characteristics in growing-finishing pigs. Journal of Animal Science, 88, 3320-3326  https://doi.org/10.2527/jas.2009-2308
Mountzouris K. C., Tsitrsikos P., Palamidi I., Arvaniti A., Mohnl M., Schatzmayr G., Fegeros K. (): Effects of probiotic inclusion levels in broiler nutrition on growth performance, nutrient digestibility, plasma immunoglobulins, and cecal microflora composition. Poultry Science, 89, 58-67  https://doi.org/10.3382/ps.2009-00308
NRC – National Research Council (1994): Nutrient requirements of poultry. 9th rev. ed. National Academies Press, Washington, DC.
Ouwehand AC, Salminen S, Isolauri E (2002): Probiotics: an overview of beneficial effects. Antonie van Leeuwenhoek 82, 279–289. https://doi.org/10.1023/A:1020620607611
Pajarillo Edward Alain B., Chae Jong Pyo, Balolong Marilen P., Kim Hyeun Bum, Park Chan-Soo, Kang Dae-Kyung (2015): Effects of probiotic Enterococcus faecium NCIMB 11181 administration on swine fecal microbiota diversity and composition using barcoded pyrosequencing. Animal Feed Science and Technology, 201, 80-88  https://doi.org/10.1016/j.anifeedsci.2015.01.011
Park JH, Kim IH (2015): The effects of the supplementation of Bacillus subtilis RX7 and B2A probiotics on the performance, blood profiles, intestinal Salmonella microflora, noxious gas emission, organ weight, and breast meat quality of broiler challenged with Salmonella typhimurium. Journal of Animal Physiology and Animal Nutrition,99, 326–334.
Patterson J., Burkholder K. (2003): Application of prebiotics and probiotics in poultry production. Poultry Science, 82, 627-631  https://doi.org/10.1093/ps/82.4.627
Reiter Katja, Eggebrecht Susanne, Drewes Barbara, Riess Michael, Weyrauch Karl (2006): Effects of Enterococcus faecium and Bacillus cereus var. toyoi on the morphology of the intestinal mucous membrane in piglets. Biologia, 61, -  https://doi.org/10.2478/s11756-006-0161-2
Roth FX, Kirchgessner M, Eidelsburger U, Gedek B (1992): Nutritive efficacy of Bacillus cereus as a probiotic in the fattening of calves. 1. Influence on fattening performance, carcass criteia and microbial activity in the small intestine. Agribiological Research-Zeitschrift fur Agrarbiologie Agrikulturchemie Okologie 45, 294–302.
SAS Institute (1996): SAS User’s Guide: Statistics. Version 7.0. SAS Institute Inc., Cary, NC.
Scharek L., Guth J., Reiter K., Weyrauch K.D., Taras D., Schwerk P., Schierack P., Schmidt M.F.G., Wieler L.H., Tedin K. (2005): Influence of a probiotic Enterococcus faecium strain on development of the immune system of sows and piglets. Veterinary Immunology and Immunopathology, 105, 151-161  https://doi.org/10.1016/j.vetimm.2004.12.022
Scharek L., Altherr B.J., Tölke C., Schmidt M.F.G. (2007): Influence of the probiotic Bacillus cereus var. toyoi on the intestinal immunity of piglets. Veterinary Immunology and Immunopathology, 120, 136-147  https://doi.org/10.1016/j.vetimm.2007.07.015
Vahjen W, Taras T, Simon O (2007): Effect of the probiotic Enterococcus faecium NCIMB10415 on cell numbers of total Enterococcus spp., E. faecium and E. faecalis in the Intestine of piglets. Current Issues of Intestinal Microbiology 8, 1–8.
Vondruskova H, Slamova R, Trckova M, Zraly Z, Pavlik I (2010): Alternatives to antibiotic growth promoters in prevention of diarrhoea in weaned piglets: a review. Veterinarni Medicina 55,199–224.
Yan L., Kim I.H. (2013): Effect of probiotics supplementation in diets with different nutrient densities on growth performance, nutrient digestibility, blood characteristics, faecal microbial population and faecal noxious gas content in growing pigs. Journal of Applied Animal Research, 41, 23-28  https://doi.org/10.1080/09712119.2012.739092
Zamanzad-Ghavidel S, Nazer Adl K, Maheri Sis N, Aharizad S, Mirzaei Aghsaghali A, Mohammadian M, Siadati SA. (2011): Effects of lactobacillus-based probiotic on growth performance, mortality rate and carcass yield in broiler chickens. Annals of Biological Research 2, 325–331.
Zhang Z. F., Kim I. H. (): Effects of probiotic supplementation in different energy and nutrient density diets on performance, egg quality, excreta microflora, excreta noxious gas emission, and serum cholesterol concentrations in laying hens. Journal of Animal Science, 91, 4781-4787  https://doi.org/10.2527/jas.2013-6484
Zhang Z.F., Cho J.H., Kim I.H. (2013): Effects of Bacillus subtilis UBT-MO2 on growth performance, relative immune organ weight, gas concentration in excreta, and intestinal microbial shedding in broiler chickens. Livestock Science, 155, 343-347  https://doi.org/10.1016/j.livsci.2013.05.021
Zhang Z.F., Lee J.M., Kim I.H. (2014): Effects of Enterococcus faecium DSM 7134 on weanling pigs were influenced by dietary energy and crude protein density. Livestock Science, 169, 106-111  https://doi.org/10.1016/j.livsci.2014.09.022
Zheng Aijuan, Luo Jianjie, Meng Kun, Li Jianke, Zhang Shu, Li Ke, Liu Guohua, Cai Huiyi, Bryden Wayne L, Yao Bin (2014): Proteome changes underpin improved meat quality and yield of chickens (Gallus gallus) fed the probiotic Enterococcus faecium. BMC Genomics, 15, 1167-  https://doi.org/10.1186/1471-2164-15-1167
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