Blood chemistry reference intervals of captive Asiatic black bears (Ursus thibetanus) J.J., Jeong D.H., Um S.M., Lee A.N., Song D.J., Kim S.B., Yang J., Yun Y., Lim Y.K. (2017): Blood chemistry reference intervals of captive Asiatic black bears (Ursus thibetanus). Veterinarni Medicina, 62: 533-540.
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Data on blood chemistry values can make fundamental contributions to our understanding of physiological changes. However, there is a lack of information regarding blood chemistry in Asiatic black bears (Ursus thibetanus). Thus, the objects of this study were to determine reference ranges for 29 blood chemistry variables, and to evaluate differences between age groups and between seasons. Blood samples (n = 138) were collected from 44 (20 males, 24 females; age range, 1–15 years) clinically healthy, captive Asiatic black bears (Ursus thibetanus) in the Republic of Korea. Young and adult bears showed significantly higher levels of creatinine and total cholesterol, and lower levels of blood urea nitrogen, blood urea nitrogen/creatinine ratio, lactate dehydrogenase and creatine kinase MB during hibernation compared to during non-hibernation. Adults also showed significantly higher levels of triglyceride, but lower levels of inorganic phosphorus, aspartate transaminase, alkaline phosphatase, high density lipoprotein cholesterol and creatine phosphokinase during hibernation than during non-hibernation. During hibernation, the urea nitrogen/creatinine ratio and levels of alkaline phosphatase, lactate dehydrogenase, and creatine phosphokinase in young bears were significantly higher than in adults, whereas creatinine levels were lower than in adults. During non-hibernation, the urea nitrogen/creatinine ratio and levels of calcium, alkaline phosphatase, lactate dehydrogenase, creatine phosphokinase and creatine kinase MB in young bears were significantly higher, whereas creatinine, total protein, albumin, gamma-glutamyl transferase and haemoglobin levels were lower than in adults. The results of this study provide reference values that will aid in understanding the physiology of Asiatic black bears and in assessing the health of these animals in captive environments.

Arinell Karin, Sahdo Berolla, Evans Alina L., Arnemo Jon M., Baandrup Ulrik, Fröbert Ole (2012): Brown Bears (Ursus arctos) Seem Resistant to Atherosclerosis ­Despite Highly Elevated Plasma Lipids during Hibernation and Active State. Clinical and Translational Science, 5, 269-272
Barboza Perry S., Farley Sean D., Robbins Charles T. (1997): Whole-body urea cycling and protein turnover during hyperphagia and dormancy in growing bears ( Ursus americanus and U . arctos ). Canadian Journal of Zoology, 75, 2129-2136
Bossart GD, Reindarson TH, Dierauf LA, Duffield DA (2001): CRC Handbook of marine mammal medicine. In: Dierauf LA, Gulland FMD (eds): Clinical Pathology. 2nd edn. CRC Press, Florida. 383–436.
Brannon Robert D. (1985): Serum Chemistry of Central and Northern Alaska Grizzly Bears. The Journal of Wildlife Management, 49, 893-
Brown DC, Mulhausen RO, Andrew DJ (1971): Renal function in anesthetized dormant and active bears. American Journal of Physiology 220, 293–298.
Carrero JJ, Stenvinkel P (2010): Inflammation in end-stage renal disease – what have we learned in 10 years? Seminars in Dialysis 23, 498–509.
Chauhan Ved, Sheikh Ashfaq, Chauhan Abha, Tsiouris John, Malik Mazhar, Vaughan Michael (2002): Changes during hibernation in different phospholipid and free and esterified cholesterol serum levels in black bears. Biochimie, 84, 1031-1034
Derocher Andrew E., Nelson Ralph A., Stirling Ian, Ramsay Malcolm A. (1990): EFFECTS OF FASTING AND FEEDING ON SERUM UREA AND SERUM CREATININE LEVELS IN POLAR BEARS. Marine Mammal Science, 6, 196-203
Duncan JR, Prasse KW, Mahaffey EA (1994): Proteins, lipids, and carbohydrates. In: Duncan JR, Prasse KW, Mahaffey EA (eds): Veterinary Laboratory Medicine: Clinical Pathology. 3rd edn. Iowa State University Press, Iowa. 300 pp.
Friedrichs Kristen R., Harr Kendal E., Freeman Kathy P., Szladovits Balazs, Walton Raquel M., Barnhart Kirstin F., Blanco-Chavez Julia (2012): ASVCP reference interval guidelines: determination of de novo reference intervals in veterinary species and other related topics. Veterinary Clinical Pathology, 41, 441-453
Geffré Anne, Friedrichs Kristen, Harr Kendal, Concordet Didier, Trumel Catherine, Braun Jean-Pierre (2009): Reference values: a review. Veterinary Clinical Pathology, 38, 288-298
Græsli Anne Randi, Fahlman Åsa, Evans Alina L, Bertelsen Mads Frost, Arnemo Jon Martin, Nielsen Søren Saxmose (2014): Haematological and biochemical reference intervals for free-ranging brown bears (Ursus arctos) in Sweden. BMC Veterinary Research, 10, -
Græsli Anne Randi, Evans Alina L., Fahlman Åsa, Bertelsen Mads F., Blanc Stéphane, Arnemo Jon M. (2015): Seasonal variation in haematological and biochemical variables in free-ranging subadult brown bears (Ursus arctos) in Sweden. BMC Veterinary Research, 11, -
Hellgren E. C., Vaughan M. R., Kirkpatrick R. L., Scanlon P. F. (1990): Serial Changes in Metabolic Correlates of Hibernation in Female Black Bears. Journal of Mammalogy, 71, 291-300
Hellgren EC, Rogers LL, Seal US (1993): Serum chemistry and hematology of black bears: physiological indices of habitat quality or seasonal patterns? Journal of Mammalogy 74, 304–315.
Hissa R, Siekkinen J, Hohtola E, Saarela S, Hakala A, Pudas J (1994): Seasonal patterns in the physiology of the European brown bear (Ursus arctos arctos) in Finland. Comparative Biochemistry and Physiology. Part A, Molecular and Integrative Physiology 109, 781–791.
Hissa R, Hohtola E, Tuomala-Saramaki T, Laine T, Kallio H (1998): Seasonal changes in fatty acids and leptin contents in the plasma of the European brown bear (Ursus arctos arctos). Annales Zoologici Fennici 35, 215–224.
Huber Djuro, Kusak Josip, Žvorc Zdravko, Rafaj Renata Barić (1997): EFFECTS OF SEX, AGE, CAPTURING METHOD, AND SEASON ON SERUM CHEMISTRY VALUES OF BROWN BEARS IN CROATIA. Journal of Wildlife Diseases, 33, 790-794
Jani A., Martin S. L., Jain S., Keys D., Edelstein C. L. (2013): Renal adaptation during hibernation. AJP: Renal Physiology, 305, F1521-F1532
Kamine A, Shimozuru M, Shibata H, Tsubota T (2012a): Changes in blood glucose and insulin responses to intravenous glucose tolerance tests and blood biochemical values in adult female Japanese black bears (Ursus thibetanus japonicus). Japanese Journal of Veterinary Research 60, 5–13.
Kamine A, Shimozuru M, Shibata H, Tsubota T (2012b): Effects of intramuscular administration of tiletamin-zolazepam with and without sedative pretreatment on plasma and serum biochemical values and glucose tolerance test results in Japanese black bears (Ursus thibetanus japonicus). American Journal of Veterinary Research 73, 1282–1289.
Lee J., Ronald K., Oritsland N. A. (1977): Some Blood Values of Wild Polar Bears. The Journal of Wildlife Management, 41, 520-
Lohuis T D, Beck T D.I, Harlow H J (2005): Hibernating black bears have blood chemistry and plasma amino acid profiles that are indicative of long-term adaptive fasting. Canadian Journal of Zoology, 83, 1257-1263
Matula George J., Lindzey James S., Rothenbacher H. (1980): Sex, Age, and Seasonal Differences in the Blood Profile of Black Bears Captured in Northeastern Pennsylvania. Bears: Their Biology and Management, 4, 49-
Mazzucco Sara, Agostini Francesco, Mangogna Alessandro, Cattin Luigi, Biolo Gianni (2010): Prolonged Inactivity Up-Regulates Cholesteryl Ester Transfer Protein Independently of Body Fat Changes in Humans. The Journal of Clinical Endocrinology & Metabolism, 95, 2508-2512
McGee-Lawrence Meghan E., Wojda Samantha J., Barlow Lindsay N., Drummer Thomas D., Bunnell Kevin, Auger Janene, Black Hal L., Donahue Seth W. (2009): Six months of disuse during hibernation does not increase intracortical porosity or decrease cortical bone geometry, strength, or mineralization in black bear (Ursus americanus) femurs. Journal of Biomechanics, 42, 1378-1383
Mominoki Katsumi, Morimatsu Masami, Karjalainen Minna, Hohtola Esa, Hissa Raimo, Saito Masayuki (2005): Elevated plasma concentrations of haptoglobin in European brown bears during hibernation. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 142, 472-477
Nelson RA (1980): Protein and fat-metabolism in hibernating bears. Federation proceedings 39, 2955–2958.
Nelson RA, Jones JD, Wahner HW, McGill DB, Code CF (1975): Nitrogen metabolism in bears: urea metabolism in summer starvation and in winter sleep and role of urinary bladder in water and nitrogen conservation. Mayo Clinic Proceedings 50, 141–146.
Nelson RA, Steiger DL, Beck TDI (1983): Neuroendocrine and metabolic interactions in hibernating black bear. Acta Zoologica Fennica 174, 137–141.
Nelson R., Beck T., Steiger D. (1984): Ratio of serum urea to serum creatinine in wild black bears. Science, 226, 841-842
Ramsay Malcolm A., Nelson Ralph A., Stirling Ian (1991): Seasonal changes in the ratio of serum urea to creatinine in feeding and fasting polar bears. Canadian Journal of Zoology, 69, 298-302
Rosol TJ, Capen CC (1997): Calcium-regulating hormones and diseases of abnormal mineral (calcium, phosphorus, magnesium) metabolism. In: Kaneko JJ, Harvey JW, Bruss ML (eds): Clinical Biochemistry of Domestic Animals. 5th edn. Academic Press, San Diego. 619–702.
Schroeder Mark T. (1987): Blood Chemistry, Hematology, and Condition Evaluation of Black Bears in Northcoastal California. Bears: Their Biology and Management, 7, 333-
Shimozuru Michito, Kamine Akari, Tsubota Toshio (2012): Changes in expression of hepatic genes involved in energy metabolism during hibernation in captive, adult, female Japanese black bears (Ursus thibetanus japonicus). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 163, 254-261
Stenvinkel Peter, Fröbert Ole, Anderstam Björn, Palm Fredrik, Eriksson Monica, Bragfors-Helin Ann-Christin, Qureshi Abdul Rashid, Larsson Tobias, Friebe Andrea, Zedrosser Andreas, Josefsson Johan, Svensson My, Sahdo Berolla, Bankir Lise, Johnson Richard J., Burdmann Emmanuel A. (2013): Metabolic Changes in Summer Active and Anuric Hibernating Free-Ranging Brown Bears (Ursus arctos). PLoS ONE, 8, e72934-
Stenvinkel P, Jani AH, Johnson RJ (2013b): Hibernating bears (Ursidae): metabolic magicians of definite interest for the nephrologist. Kidney International 83, 207–212.
Stockham SL, Scott MA (eds) (2008): Fundamentals of Veterinary Clinical Pathology. 2nd edn. Blackwell Publishing, Ames. 908 pp.
Storm Gerald L., Alt Gary L., Matula George J., Nelson Ralph A. (1988): BLOOD CHEMISTRY OF BLACK BEARS FROM PENNSYLVANIA DURING WINTER DORMANCY. Journal of Wildlife Diseases, 24, 515-521
Tryland Morten, Brun Edgar, Derocher Andrew E., Arnemo Jon M., Kierulf Peter, Ølberg Rolf-Arne, Wiig Øystein (2002): PLASMA BIOCHEMICAL VALUES FROM APPARENTLY HEALTHY FREE-RANGING POLAR BEARS FROM SVALBARD. Journal of Wildlife Diseases, 38, 566-575
Vestergaard Peter, Støen Ole-Gunnar, Swenson Jon E., Mosekilde Leif, Heickendorff Lene, Fröbert Ole, Burne Thomas (2011): Vitamin D Status and Bone and Connective Tissue Turnover in Brown Bears (Ursus arctos) during Hibernation and the Active State. PLoS ONE, 6, e21483-
Wright PA (1995): Nitrogen excretion: three end products, many physiological roles. Journal of Experimental Biology 198, 273–281.
Wright Patricia A., Obbard Martyn E., Battersby Brendan J., Felskie Andrew K., LeBlanc Paul J., Ballantyne James S. (1999): Lactation during Hibernation in Wild Black Bears: Effects on Plasma Amino Acids and Nitrogen Metabolites. Physiological and Biochemical Zoology, 72, 597-604
Yanagibori R, Suzuki Y, Kawakubo K, Kondo K, Iwamoto T, Itakura H, Makita Y, Sekiguchi C, Gunji A, Kondou K (1997): The effects of 20 days bed rest on serum lipids and lipoprotein concentrations in healthy young subjects. Journal of Gravitational Physiology 4, 82–90.
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