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Salinity represents a significant abiotic stress that markedly influences plant growth through osmotic stress induction. Plants commonly undergo osmotic adaptation when subjected to prolonged periods of saline stress. The current experiments were conducted on five wheat (Triticum aestivum L.) genotypes with contrasting salt tolerance capacities – Mirbashir 128, Gobustan, Gyzyl bughda, Fatima, and Zirva 80 under salinity stress caused by 150 mmol NaCl. The relative water content and osmotic potential were found to decrease significantly in salinity-sensitive genotypes (Fatima and Zirva 80) compared to salinity-tolerant ones (Mirbashir 128, Gobustan, and Gyzyl bughda) when treated with 150 mmol NaCl. Salinity also caused the accumulation of soluble sugars and proline, the amounts of which were observed to be higher in salinity-tolerant genotypes than sensitive ones, while lipid peroxidation was higher in salinity-sensitive genotypes. In salinity-tolerant genotypes, 150 mmol NaCl caused increased antioxidant enzyme activities and accumulation of flavonoids, including anthocyanins, confirming the rapid development of the stress reactions in these plants. Differences in the osmoregulation indicators and antioxidant responses between salinity-tolerant and sensitive plants are assumed to be related to their salinity-tolerance traits. This investigation provides pivotal foundational insights for enhancing the salt tolerance of wheat genotypes, thereby potentially enhancing both yield and quality in diverse wheat cultivars thriving in saline environments.

The potential use of volatile compounds released from milled seeds of mustard (Brassica juncea cv. Malopolska) obtained from three different companies was tested in in vitro and in vivo experiments for their inhibitory effect on Botrytis cinerea growth on agar media and its infection on vegetable leaves of cucumber, bean and spinach. In the experiments with spinach, the effect of volatiles from mustards on the storage and sensory quality of fumigated leaves was evaluated. The antifungal effect of the volatiles depended on the source and dosage of mustard seeds and biofumigation time. The most efficient inhibition of B. cinerea mycelium growth on agar media and vegetable leaves was mustard S from SHR company. The development of grey mould on spinach leaves was inhibited in the treatment with 4 h biofumigation with the volatiles from mustard S seeds in experiments conducted at 10 °C and also at 18 °C. In the sensory and storage quality analysis, the spinach leaves treated with volatiles from mustard seeds showed acceptable parameters that predisposed the product to consumption. The results show that it is possible to reduce the incidence of vegetable grey mould with the treatment of milled mustard seeds, opening a potential application of biofumigation in the control of B. cinerea in vegetables.

Coffee leaf rust (CLR), Hemileia vastatrix, is a disease that has severely impacted the major coffee growing areas of the world. The discovery of CLR in Hawaii in October 2020 was devastating news to local growers, as it quickly spread to all coffee-growing areas, threatening the second-highest-valued crop in the State. Although the fungicide Priaxor Xemium had been approved as a Section 18 emergency exemption, there was still an urgent need for true systemic fungicides to provide sustained control of the disease. The study's overall objective was to test the efficacy of various systemic fungicides against CLR in field conditions. During the coffee growing season for two years, various fungicides were tested on two farms in the Kona district of Hawaii. Treated trees showed an 84% reduction in CLR incidence compared to untreated trees in Year 1 and a 79% reduction in Year 2 by the end of the study. Treated trees also had 12% and 38% more total leaves per branch than untreated trees in Years 1 and 2, respectively. All fungicide treatments provided excellent or good control of CLR compared to the untreated control under the climate of Hawaii.

Cerebellar hypoplasia caused by feline panleukopenia virus (FPV) is well documented in domestic cats. Still, it remains unreported in wild felid species, including the Amur leopard cat (Prionailurus bengalensis euptilura). Understanding the impact of such viral diseases on wild populations is crucial for advancing conservation efforts and protecting wildlife. An orphaned Amur leopard cat exhibiting idiopathic ataxia was rescued. Initial diagnostics, including physical examination, radiography, and blood analysis, yielded no remarkable findings, though its clinical signs indicated an underlying neurological problem. Subsequent real-time polymerase chain reaction tests detected FPV. Magnetic resonance imaging (MRI) revealed brain lesions, including reduced cerebellar parenchyma and cerebrospinal fluid occupying the space where the cerebellum should be located. These findings suggested cerebellar hypoplasia caused by in utero FPV infection. The Amur leopard cat was euthanised owing to its permanent disability, and the necropsy confirmed a markedly shrunken cerebellum. At the same time, histopathology identified decreased cellularity of the molecular and granular layers of the cerebellar cortex. These results coincided with the MRI findings. This report suggests that cerebellar hypoplasia caused by FPV can occur in wild felid species.

Sustainable cropping systems require efficient usage of fossil energy. This study performed on a long-term field experiment in the Pannonian Basis investigated the energy efficiency of four tillage systems (mouldboard plough (MP), deep conservation tillage (CT_d), shallow conservation tillage (CT_s) and no-tillage (NT)) for sugar beet and soybean production, taking fuel consumption, total energy input (made up of both direct and indirect inputs), crop yield, energy output, net-energy output, energy intensity and energy use efficiency into account. The input rates of fertiliser, chemical plant protection, and seeds were set constant across years; whereas measured values of fuel consumption were used for all tillage treatments. NT required a considerably lower energy input than MP and CT_d as no fuel is needed for tillage and just slightly more fuel for additional spraying of glyphosate. Anyhow, the energy efficiency parameters did not differ between tillage treatments, as theses parameters were mainly determined by energy output, which was considerably higher than the energy input. However, year effects on the energy efficiency were observed for both crops. Nitrogen fertilisation and diesel fuel consumption were identified as the most energy-intensive inputs. Consequently, the energy input for sugar beet was higher than that for soybean, which was identified as a low-input crop. But sugar beet attained a more than 4 times higher net-energy output, a 2.5 times higher energy use efficiency, and an energy intensity for yield production of less than 3 times those of soybean.

China’s local chicken breeds, especially those in the Zhejiang Province, are rich in genetic diversity owing to environmental heterogeneity and complex ecosystems. Distinctive local breeds have emerged through long-term natural selection and domestication. We investigated the genetic diversity and population structure of local chickens in southern Zhejiang and surrounding areas using whole-genome resequencing of 129 chickens from seven populations. A total of 1.8 terabytes of raw data was obtained, and 4 802 728 single nucleotide polymorphisms were detected. The Xianju chicken population exhibited the highest genetic diversity, while Yandang Partridge chickens were genetically distant from other chicken breeds. This study provides valuable information for conserving poultry genetic diversity and informs about breeding programmes of local Chinese chicken breeds.

Conditioning films on surfaces employed in the dairy industry serve as the precursors to the formation of pathogenic biofilms that impact product quality and consumer safety. Conditioning films have been studied from several aspects. However, there has been no study that evaluated the effect of raw milk conditioning film on the adhesion of Escherichia coli. This study investigated the adhesion of E. coli on glass and stainless-steel surfaces conditioned with raw milk and explored the surface properties potentially influencing this adhesion using the contact angle method. The results showed that after treating surfaces with raw milk, the adhesion of the bacteria on stainless steel and glass was significantly altered. Adhesion increased significantly on stainless steel (from 0.55 log₁₀ to 2.8 log₁₀) but it decreased on glass (from 1.56 log₁₀ to 0.8 log₁₀). Significant alterations were observed in the physicochemical properties of the surfaces. Glass was initially relatively hydrophilic (46.33°), while stainless steel was relatively hydrophobic (82.5°). After treatment, the glass became relatively more hydrophobic (74.6°), and stainless steel became relatively more hydrophilic (69.4°). The electron donor/acceptor components of glass decreased after the treatment, while these components increased for stainless steel. The significant changes in adhesion were hypothesized to be due to the modification of surface properties by the raw milk.

The global focus on the relationship between digitisation and agricultural carbon emissions remains high. However, research on the systemic ramifications of comprehensive digital policy implementation remains limited. Against the backdrop of China's pursuit of carbon neutrality and carbon emission peaking targets, we employed the difference-in-differences method to investigate the impact of applying a digital policy on agricultural carbon emissions. Our findings indicated that the implementation of the National Big Data Comprehensive Pilot Zone policy could effectively mitigate agricultural carbon emissions, resulting in a sustained positive influence. The intermediary mechanism test results validated the beneficial effects of financial expenditures on science and technology, as well as the number of information practitioners. The regional heterogeneity analysis results revealed that the policy effect was obvious in the major grain-producing areas but not in the major grain-selling areas or production–marketing balance areas. Additionally, differences in policy effectiveness were observed across different crop types. This study not only offers valuable insights for agricultural carbon reduction in China but also provides robust case data and guidance for other developing countries worldwide in the formulation and execution of digital policies aimed at promoting agricultural carbon emission reduction.

The teak bee-hole borer [Xyleutes ceramica (Walker, 1865)] is considered one of the most serious pests of teak (Tectona grandis) in Thailand. The present study investigates climatic and topographic variables affecting the infestation of teak trees by X. ceramica in 10 plantations and predicts the risk of infestation by the species under current and future climatic conditions in Thailand. At each plantation, 48 plots evenly distributed among twelve teak stands were sampled. The infested teak trees in the plots were assessed, and the coordinates of the tree positions were recorded. The maximum entropy (MaxEnt) model was used to assess the effects of environmental factors and predict the occurrence probability of the species using current and projected (2050) climate data based on the Shared Socioeconomic Pathways SSP1-2.6 and SSP5-8.5 scenarios from multiple global climate models. According to our results, high accuracy values [AUC (area under the curve) = 0.852, TSS (true skill statistics) = 0.775] of the model prediction were obtained, and the infestation was found to be driven much more by climate than by topographic characteristics. Above all, X. ceramica was found to prefer moderate temperatures in a highly distinct seasonal climate. Additionally, relatively low amounts of premonsoon rainfall are also found to be favoured by the species. The predicted risk map revealed that the northern region is the core area of X. ceramica infestation in Thailand under current and future climatic conditions, but the severity of infestation is predicted to gradually decrease under the predicted future climatic conditions. Recommendations for management to minimise tree damage caused by X. ceramica are also presented in this study.

In an era marked by an ageing agricultural workforce and intensifying environmental pressures, agricultural infrastructure plays a crucial role in enhancing green productivity. This study investigates the impact of agricultural infrastructure construction and population ageing on agricultural green total factor productivity (agricultural GTFP), utilising provincial panel data from 2004 to 2022 in China. Our findings reveal that the agricultural infrastructure construction significantly boosts agricultural GTFP and significantly mitigates the negative effects of ageing on agricultural green production at the 0.01 level. Notably, different types of infrastructure exhibit varying impacts, with electricity and irrigation infrastructure demonstrating the most significant positive effects. We further identify a strong mediating effect of farmland transfer and agricultural mechanisation in facilitating infrastructure-driven agricultural GTFP growth. Our analysis reveals a pronounced regional heterogeneity, with non-food producing, central, western regions of China benefiting most from agricultural infrastructure investments. To address the challenges posed by ageing, we recommend giving priority to expanding agricultural infrastructure construction, fostering agricultural socialised services, and tailoring infrastructure programs to regional resource endowments. These strategies aim to compensate for labour shortages, enhance resource utilisation, and ultimately drive improvements in agricultural green productivity.

In recent years, epidemics of wheat yellow rust caused by Puccinia striiformis f. sp. tritici (Pst) have been observed in major winter wheat-producing regions in Kazakhstan. However, there is currently very little information about the racial composition and virulence of Pst. The global emergence of aggressive and genetically diverse Pst races leads to different seasonal and geographic patterns of the pathogen, making cultivated wheat varieties vulnerable to the pathogen and potentially causing yellow rust epidemics. Three periods with different characteristic dominant Pst races were distinguished in Kazakhstan during 1985–2022. The first period covers 1985–2000, when in the southeast of the country, the main Pst races were 7E156 (31/1.5), 7E158 (A-8/5), 39E158 (X/1.5) and 86E16. In the second period (2001–2010), the crops were dominated by races 7E159, 31E159 and 47E224, which showed virulence to varieties with resistance genes Yr9 and Yr18. In the third period (2018–2022), the most dominant races in the fungal population were 7E159, 39E158, 79E73, 79E179, and 111E158, exhibiting virulence to varieties with the Yr26 and Yr27 genes. In the background of field infection, the resistance genes Yr5, Yr10, and Yr15 remain reliable in ensuring resistance; the Yr4, YrSp, and YrND sources are also highly effective against the Pst population.

To promote the sustainable consumption of prepared dishes, this study examines how green attribute information (GAI) and live streaming scenes experience (LSSE) influence consumers' green perceptions (GP) – including perceived green value (PGV) and perceived green trust (PGT) – and subsequently affect their purchase intention (PI). A green perception model was developed and tested using a mixed-method approach. Word cloud analysis was conducted on consumer comments collected from e-commerce platforms to extract key descriptors and concerns, which informed the design of a structured questionnaire. A total of 291 valid responses were analysed using partial least squares structural equation modelling (PLS-SEM). Results show that: (i) GAI does not directly impact PI, indicating that GP is a necessary mediator; (ii) GAI significantly influences PGV, but not PGT; (iii) GP fully mediates the GAI–PI relationship, although PGV has a negligible direct effect on PGT; (iv) LSSE significantly enhances both GP and PI; (v) GP partially mediates the LSSE–PI relationship. These findings highlight the dual role of LSSE in facilitating information adoption and emotional engagement, and suggest that enhancing LSSE design and enriching GAI are effective strategies to increase consumers' green perception and purchase intention towards prepared dishes.

The present study investigates the differential protein expression levels between the frost-tolerant variety Qingda No.1 and the frost-sensitive variety Gannong No.9. The analysis was conducted using 4D-Label-free quantitative proteomics technology, with the samples collected prior to and after overwintering. The results showed that the protein expression of Qingda No.1 changed more significantly during the overwintering process, with 451 differentially expressed proteins (DEPs) being identified, of which 224 were up-regulated and 227 down-regulated. In contrast, the protein expression of Gannong No.9 differed from that of alfalfa roots of the frost-sensitive variety, with 204 DEPs being identified, of which 93 were up-regulated and 111 down-regulated. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that the phenylpropanoid biosynthesis pathway was significantly enriched in both groups. Furthermore, enzymes such as phenylalanine ammonia-lyase (PAL), cinnamic acid 4-hydroxylase (C4H), and chalcone isomerase (CHI) were significantly up-regulated in the freezing-tolerant varieties. Protein interaction network analysis revealed the synergistic regulation mode of DEPs. The present study elucidated the metabolic adaptive mechanism of overwintering in alfalfa roots at the protein level, thus providing a theoretical basis for the selection and breeding of frost-tolerant varieties.

The present investigation was an attempt to compare the phytoceutic potential of ginger rhizome and ginger leaves of the Suravi variety. For this purpose, both rhizome and leaves were dried and used for the preparation of patties. After that, patties were assessed for colour tonality, texture, total phenolic content and hedonic response such as colour, taste, flavour, texture and overall acceptability. The results depicted that L* and b* values changed significantly during the storage interval; however, b* value was also affected by treatments whilst L* and a* values did not impart any momentous effect. For texture, the highest value was observed for patties with ginger rhizome powder (0.067 ± 0.0032 N) followed by patties with ginger leaf powder (0.060 ± 0.0029 N) and then control patties (0.057 ± 0.0026 N). For total phenolic content (TPC), maximum phenolic contents were observed as 84.80 ± 3.31 mg GAE 100 g^-1 in treatment T₂ followed by 75.68 ± 2.95 mg GAE 100 g^-1 in T₁ and 61.70 ± 2.41 mg GAE 100 g^-1 in T₀. For hedonic response, all the parameters changed significantly during the storage interval; however, flavour, taste and overall acceptability changed momentously with treatments. The findings of the current investigation demonstrated that ginger leaves have a higher antioxidant potential as compared to the ginger rhizome and control patties, and they should be incorporated into food products.

The aim of the study was to prepare and test the effect of diets with 50% and 100% replacement of soybean meal with dehulled seeds of white lupin (Lupinus albus) variety Zulika. One hundred and eighty Cherry Valley duckling were divided to control group (C) and two experimental groups E₅₀ (50% replacement of soybean meal with dehulled white lupin meal) and E₁₀₀ (100% replacement of soybean meal with dehulled white lupin meal). There were evaluated the production parameters and the amino acid spectrum of breast and thigh muscles up to 42 days of age. The effect of diets containing lupin meal had no negative effect on the yield of duckling. In particular, positively can be evaluated higher growth rate in the experimental groups compared to the control (C 3.22 ± 0.28 kg, E₅₀ 3.40 ± 0.27 kg, E₁₀₀ 3.48 ± 0.28 kg), at the same time significantly lower mortality in the experimental groups compared to the control (C 5.88%, E₅₀ 1.47%, E₁₀₀ 1.49%) and, except for E₁₀₀, minimal differences in feed conversion ratio (C 2.33 kg/kg, E₅₀ 2.27 kg/kg, E₁₀₀ 2.71 kg/kg). Feeding diets based on lupin meal resulted in the reduction of most essential and non-essential amino acids (P ≤ 0.05) in the dry matter content of both breast and thigh muscle (E₅₀, E₁₀₀). Lupine meal-based diets positively influenced ducks’ health, which was reflected by lower mortality rate in the experimental groups. Therefore, the results show that diets based on lupin meal had a positive effect on the health and welfare of intensively fattened ducks and that they affected positively the economic aspect of breeding.

For viable papaya production, more hermaphrodite plants are needed than male and female (though they produce inferior fruits). To this end, the sex of the plants must be ascertained early in their growth cycle, which is not possible with conventional methods. Molecular marker has shown its utility for this purpose, and in this study, 100 seedlings of the CO 7 gynodioecious variety and 100 seedlings of the CO 8 dioecious variety that were released from this university were analysed for their sex using the RAPD-SCAR (randomly amplified polymorphic DNA-sequence characterised amplified region) marker, T12. In those lines under investigation, the T12 marker’s presence indicated male lines, while its absence indicated female or hermaphrodite lines. To confirm that there was no experimental error – that is, the lack of the SCAR marker was caused by the non-existence of the complementary target region – an oligomer primer called OPA 04 was initially employed as a control to validate the molecular marker system among the lines under investigation. Results of this study have shown that T12 has achieved 97% and 98% accuracy in correctly identifying the male and hermaphrodite flowers in the field in CO 7 and CO 8 accessions, respectively. A similarity search of the sequences of the polymerase chain reaction (PCR) product amplified by T12 from CO 7 and CO 8 has highlighted that they matched with male-specific regions of the papaya Y chromosome. Further, it was estimated that by employing molecularly certified papaya seedlings with predetermined sex, farmers could save up to 55% on labour costs when compared to approaches that use seedlings from conventional ways by implying the removal of male plants. Thus, the findings of this study help growers and breeders to identify sex early and guarantee profitable papaya production.

This paper reports the feasibility of cold atmospheric microwave plasma (CAMP) therapy for birds with open wounds. Four domestic pigeons (Columba livia domestica) with open wounds of varying severity were treated with CAMP as an adjunct to conventional therapy. Wound healing was assessed using a modified scoring system during each treatment session, and the extent of healing was calculated as a percentage. The results revealed variability in wound improvement across cases, influenced by the initial severity of the wounds. In some cases, the outcomes were limited due to underlying conditions that overrode the recovery process, suggesting that the extent of healing may depend more on the initial wound condition than the treatment itself. Nevertheless, other cases showed positive results in healing and recovery under CAMP therapy, highlighting its potential benefits. No adverse reactions or complications were encountered, supporting its safety for avian wound management. These findings suggest that although CAMP can potentially promote wound healing, further studies will be needed to establish standardised treatment protocols.

In this study the eradication effectiveness of four commonly used disinfectants against benzalkonium chloride (BC) adapted and non-adapted biofilms of Listeria monocytogenes was compared and the effects of food preservatives on the growth of these strains were comparatively evaluated on pasteurised chicken sausage. After BC adaptation, the minimum inhibitory concentrations (MICs) of BC against planktonic bacteria of L. monocytogenes increased, while the MICs of chlorine dioxide remained unchanged. BC adapted strains showed stronger biofilm formation than the wild-type parents. When used at 1 × MIC, the eradication rates of chlorine dioxide on biofilm biomass, cell viability and biofilm extracellular polymeric substance were higher than BC. When used at the recommended concentrations, chlorine dioxide exhibited the highest efficiency in BC adapted and non-adapted biofilm eradication. Among the four food preservatives, nisin showed the highest inhibition of both BC adapted and non-adapted strains grown on pasteurised chicken sausage. Our data suggest that proper use of BC is required to reduce the exposure of L. monocytogenes to sublethal concentrations of BC and the emergence of BC adapted strains.

Aflatoxins are considered the most toxic secondary metabolites of concern to food safety due to their wide distribution and high toxicity in foods and feeds. The aim of this study is to identify the prevalence of aflatoxin M₁ (AFM₁) in Van Herby cheeses (brined/dry salted). A total of 90 brined and dry salted Van Herby cheese samples offered for retail sale were analysed. The AFM₁ level in the samples was determined by the chromatographic [High-Performance Liquid Chromatography (HPLC)/Fluorescent Detection (FLD)] method. Brined Van Herby cheese samples contained AFM₁ in amounts ranging from < LOD to 0.573 ng·g^–1 with a mean of 0.165 ± 0.206 ng·g^–1, while dry salted Van Herby cheese samples contained < LOD to 0.017 ng·g^–1 AFM₁. The analysis of the prevalence of AFM₁ in brined and dry salted Van Herby cheese samples was 17.78% (n = 8) and 2.22% (n = 1), respectively. In Van Herby cheese production, standardisation, quality improvement and food safety control procedures need to be used effectively and disseminated. In addition to good agricultural and storage practices to prevent mycotoxin formation, measures must be taken to prevent aflatoxin contamination in animal feed. These applications and systems will provide positive contributions in terms of total quality, nutrients and public health, as well as different advantages such as technological superiority.

The study focused on the relationship between leaf-to-fruit ratio (LFR) and walnut kernel quality in Juglans regia ‘Wen 185’. It was investigated how LFR influences single kernel weight, the contents and percentages of organic matter in kernels, the 13C distribution proportion (¹³C_DP) in crude fat and protein, and the number and size of oil bodies within the kernels. A gradually decreasing LFR led to reduced single kernel weight, the contents of crude fat, crude protein, and soluble sugar dramatically (P < 0.05), with no significant changes in the percentages and (¹³C_DP) of crude fat, crude protein, and soluble sugar (P > 0.05). Moreover, there were no significant differences in the number of oil bodies per unit area and the size of oil bodies in kernel cotyledons and endosperm storage cells among the different LFR (P > 0.05). We propose that the walnut kernel quality depends on the proportion of sugar converted into fat and protein in the kernels, that the changes in LFR affect the amount of sugar accumulated in kernels but not the proportion of sugar converted to fat and protein, and that the LFR, therefore, have no effect on the percentages of crude fat and crude protein in walnut kernels.

Banana quality is influenced by many factors, including variety and level of fruit ripeness. This quality can be evaluated from various points of view, one of which is the rheological consideration. Rheological properties are very important to study because they determine the design of equipment and processes, and minimise product damage. The aim of this research was to analyse and model the effect of variety and ripeness level on the rheological properties of banana fruit by applying a creep test. This research was carried out using a factorial experimental design 3 × 3 with 5 replications. Three varieties of banana, namely Ambon (Musa parasidiaca var. sapientum L. Kunt.), Raja (Musa parasidiaca L.), and Kepok (Musa acuminata balbisiana Colla) and each in three levels of ripeness, namely mature green, half ripe, and ripe. It was found that the parameters of the rheological properties of bananas changed according to the ripeness level (P < 0.05). The values of these rheological parameters decreased as the bananas ripened. Meanwhile, the variety and the interaction between variety and ripeness had no significant influence on the rheological parameters measured (P > 0.05). The constants of the four-element Burger model changed with the ripeness in all banana varieties. The Burger model with four elements could accurately predict the strain value of bananas tested in the creep test.

One of the disorders of the mammary gland in the queen is feline mammary fibroadenomatous hyperplasia (FMFH), caused by an increasing concentration of progesterone (P4) and some other local growth factors. It occurs mostly during puberty after the heat characterised by spontaneous or provoked ovulation, as a result of exogenous progesterone intake and sometimes during pregnancy. To diagnose a 14-month-old intact Maine Coon queen with extensive mammary gland hyperplasia, a clinical examination, analyses of the progesterone (P4) concentrations and ultrasound examination were performed. Feline mammary fibroadenomatous hyperplasia associated with a high P4 concentration after spontaneous ovulation was confirmed. After 24 days of therapy with a progesterone antagonist, aglepristone, the symptoms of FMFH resolved. After the next eight weeks, the queen was mated after the owner’s decision. In the third week of pregnancy, a relapse was detected (mammary gland enlargement, pain, discomfort). At the same time, no abnormalities in the uterus or embryos were detected via ultrasound. The P4 concentrations were under regular control. For the next two weeks, only conservative treatment with NSAIDs was used. The queen spontaneously delivered six kittens without any difficulties or perinatal complications 67 days after the first mating. The cat previously treated with aglepristone for FMFH was successfully bred, but FMFH symptoms returned when progesterone concentrations increased during pregnancy.

Carpomyia vesuviana Costa, a fruit fly species, is a major pest affecting the jujube (Ber). This monophagous insect pest causes significant economic losses in regions where the jujube is cultivated. Hence, the present study was conducted to provide a detailed morphometric and molecular analysis of C. vesuviana across various Indian regions, specifically Tamil Nadu, Punjab, and Rajasthan. Morphometric measurements were conducted for various developmental stages, from the egg to adult. The overall mean length and width of the egg were 0.61 mm and 0.18 mm, respectively, and the fully grown maggot measured 6.19 mm (length) and 1.91 mm (width). The length and width of the prepupa and pupa were 7.67 mm and 1.64 mm, and 4.00 mm and 1.72 mm, respectively. The overall mean length of the female whole body and wing expanse measured 4.74 mm and 3.92 mm, respectively. The overall mean male whole body length and wing expanse were 4.19 mm and 3.56 mm, respectively. The results of the Principal Component Analysis (PCA) revealed that the maggot's length at different stages, pupa length, and egg width were the main contributors to the variability, particularly in the samples from Rajasthan. Additionally, the adult female fly's morphometric traits, such as the wing and thorax measurements, showed regional variations, with the PCA highlighting Punjab's alignment with larger head and abdomen traits. The molecular analysis based on PCR and sequencing of the COXI–COXII region confirmed the species identity. The nucleotide sequence of C. vesuviana from Tamil Nadu and Punjab was deposited in GenBank as PQ198003.1 and PQ198005.1 which had nearly 99% genetic similarity with two sequences NC_071721.1 (Beijing, China) and MT121231.1 (Beijing, China) submitted in the NCBI database. A phylogenetic analysis further demonstrated that the Tamil Nadu and Punjab populations were closely related to an Iranian sample, while samples from other countries, such as the C. schineri.

Bruguiera gymnorrhiza and Acanthus ilicifolius are key mangrove species, but their populations have declined recently due to human activities. To promote their restoration, this study optimised seed germination and seedling cultivation using the plant factory method. The parameters, such as illumination, salinity, temperature and nutrient conditions, were examined. Our results showed that suitable illumination, salinity and temperature promoted seed germination of both species. Nutrient addition promoted the germination of B. gymnorrhiza but had little effect on A. ilicifolius. Both species of seedlings grew best in short illumination duration and moderate illumination intensity. Although both species can tolerate high salinity, low salinity (0–10‰ for B. gymnorrhiza and 0–5‰ for A. ilicifolius) promoted seedlings’ growth. High temperature (28–32°C) accelerated the growth of both species of seedlings. Nutrient addition enhanced the growth of both species’ seedlings, especially the addition of Ca²⁺/Mg²⁺ and trace elements strongly promoted the growth of B. gymnorrhiza seedlings. We obtained optimal conditions for seed germination and seedling growth of both species in the plant factory, demonstrating that environmental control significantly enhanced their germination and growth rates. Our findings provide valuable insights into the efficiency of mangrove restoration and the sustainable development of mangrove ecosystems.

Control and supervision activities in the field of forest management are a type of activity of state authorities to detect, prevent forest violations. The purpose of this study is to analyse the dynamics and nature of violations in the forest sector on the example and in comparison of such regions of Russia as the Krasnodar territory and the Republic of Bashkortostan. The research methodology is selected taking into account the characteristics of the object under study. With the assistance of employees of the environmental inspectorate, search operations were organized in the areas where the greatest amount of forest damage occurs in the studied regions. The authors concluded that one of the reasons for the existing problems is the lack of interest of the state in the development of this sector of economy. The paper focuses on specific types of forest violations and the problems of their prevention. The authors believe that illegal logging belongs to the most common and socially dangerous forest violations. The article concludes that when adopting new regulations for control and supervisory activities in the field of forest management, the legislator must take into account requirements of administrative reform, regulatory guillotine, and risk-based approach.

Taking China as an example in this study, we aim to reveal the enabling effects of agricultural science and technology advancement on the progress of rural industrial incorporation at a relatively more refined level. The main innovation in this study lies in the decomposition of agricultural science and technology advancement, followed by an analysis of its effect on rural industrial incorporation along both linear and non-linear dimensions. The study results show that, along the linear dimension, agricultural science and technology advancement, agricultural knowledge advancement and agricultural technology advancement play significant driving roles in the progress of rural industrial incorporation. However, although the estimated coefficient of the integration of agricultural knowledge advancement and technology advancement is positive, it is not statistically significant. Along the non-linear dimension, agricultural science and technology advancement, agricultural knowledge advancement and agricultural technology advancement all exhibit significantly positive effects across different intervals. However, although the integration of agricultural knowledge advancement and technology advancement also shows a positive effect, its significance appears only in the second interval, indicating that the effect of this variable in promoting rural industrial incorporation can be realised only under certain conditions

Management of chronic non-healing wounds in cats requires a comprehensive approach. This report describes the treatment of a severe open skin wound on the skull using a combined approach involving allogeneic adipose-derived mesenchymal stem cells (MSCs) and autologous platelet-rich plasma (PRP). A 12-year-old neutered male mixed-breed domestic cat presented with a non-healing chronic wound on the skull. The wound extended from the orbital to the occipital area and from the left to the right temporal region. Laboratory test results were positive for feline immunodeficiency virus and impaired kidney function. Sensitivity tests revealed resistance to several antibiotics. Due to limited skin reconstruction options, MSCs were administered subcutaneously at the wound edge once a month for three months. PRP was collected one month after the initial MSC administration and injected at the wound edge monthly between MSC treatments. The wound diameter was measured daily during saline cleaning. The cat received protein-supplemented food daily. Wound healing was observed two weeks after the MSC administration, gradually decreasing in size and closing completely within 5 months. This case demonstrates the successful application of MSCs and PRP for treating chronic wounds in cats.

This experiment was conducted to study the effects of low-protein diet supplemented with exogenous protease on performance and intestinal health of broilers. A total of 560 one-day-old male Arbor Acres broiler chickens were randomly divided into 5 treatments with 8 replicates (12 birds per replicate) in a completely randomised design. The treatments were as follows: (1) maize-soybean meal basal diet (CON); (2) negative control with crude protein being 1% lower than in CON (NCON); (3) NCON + 12 000 U/kg coated alkaline protease (NCAP); (4) NCON + 16 000 U/kg alkaline protease (NAP); (5) NCON + 16 000 U/kg keratinase (NKA). The remaining 80 birds (10 replicates, 8 birds per replicate) were randomly assigned to endogenous indicator measurements. The results indicated that the NCON diet resulted in a higher feed-to-gain ratio and reduced protein digestibility, ileal amino acid digestibility, and intestinal morphological parameters (P < 0.05). Supplementation of different exogenous proteases significantly improved the apparent and true ileal digestibility of specific amino acids, enhanced jejunal chymotrypsin activity, and promoted intestinal morphological development, including increased villus height and villus height-to-crypt depth ratio (P < 0.05). Our findings suggested that the addition of exogenous protease improved the intestinal morphology of broilers.

Agricultural machines that are fully autonomous will still need human supervisors to monitor and troubleshoot system failures. Recognising the emergency as soon as possible is crucial to reduce adverse effects. The ability of humans to detect visual, auditory, or tactile cues is usually enabled by warning systems. The effectiveness of different warning cues varies in terms of prompting a quick response. The study’s objective was to compare the effectiveness of two bimodal warnings (i.e., visual-auditory and visual-tactile) at eliciting supervisor perception (which equates to level one situation awareness). Twenty-five participants engaged in an autonomous sprayer simulation. Two realistic remote supervision scenarios (i.e., in-field and close-to-field) were used to examine two bimodal warning cues: (i) visual-auditory and (ii) visual-tactile. The effectiveness of each bimodal warning was assessed based on two measures: (i) response time and (ii) noticeability. There was no significant difference between the bimodal warning cues in terms of response time when tractor sound was present in the experimental environment (reflecting the in-field remote supervision scenario); however, visual-tactile cues yielded shorter response times than visual-auditory cues when the experimental environment was quiet (reflecting the close-to-field remote supervision scenario). There were no statistically significant differences between visual-auditory and visual-tactile warnings concerning noticeability. Participants’ subjective answers indicated they preferred the visual-tactile cues better than the visual-auditory cues. It is concluded that visual-tactile warnings are preferred over visual-auditory warnings to enable perception during remote supervision of autonomous agricultural machines (AAMs).

Haemotropic mycoplasmas can cause severe anaemia in pets and also have zoonotic potential. The determination of these microorganisms from clinical samples is of critical importance. In this study, the presence of Mycoplasma haemofelis (Mhf), Candidatus Mycoplasma haemominutum (CMhm), and Candidatus Mycoplasma turicensis (CMt) in blood samples collected from 62 cats was investigated. Haemotropic mycoplasmas were identified by PCR amplifying the 16S rRNA gene, and sequence analysis was applied for confirmation and differentiation of the species. Six (9.67%) blood samples were positive for haemotropic mycoplasmas. CMhm and CMt were identified in five (8.06%) samples and one (1.61%) sample, respectively. Mhf was not detected in the samples. Additionally, a co-infection was not observed in any of the cats. It was found that the PCR positivity was not related to the sex or clinical status of the cats. To our knowledge, this is the first report investigating haemotropic mycoplasmas in blood samples from cats in Siirt Province of Türkiye. It is suggested that the data obtained from this study will contribute to clinicians working on pet animal health in this province.