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Comparison of the growth of the provenances of silver fir (Abies alba Mill.) on research plots in the regions Novohradské hory and Èeskomoravská vrchovina at the age of 50 yearsOriginal PaperJiøí Èáp, Martin Fulín, Franti¹ek Beran, Jaroslav Dostál, Martina KomárkováJ. For. Sci., 2024, 70(1):14-23 | DOI: 10.17221/113/2023-JFS The study evaluates the results of a long-term research trial lasting 50 years in two research plots established in the 1970s aimed at solving the decline of the silver fir in the Czech Republic. A total of 103 different provenances occurring naturally across 12 countries were planted in research plots No. 67, Èernovice (Èeskomoravská vrchovina), and No. 77, Nové Hrady (Novohradské hory). The total tree height, diameter at breast height (DBH) and assessment of the health status of all trees in the area were measured with the same methodology. Subsequently, the volume of large wood was calculated by the volume equation, and the growing stock per hectare was recalculated. Based on our analysis of average height growth, it was found that trees with the best growth came from provenances that included the Czech provenances CZ 70 – ®dírec nad Doubravou, CZ 82 – Vizovice, CZ 42 – Lukov, CZ 76 – Nýrsko, Suchý Kámen, and CZ 53 – Opoèno. The last three mentioned provenances also dominated in other growth parameters (DBH and volume of large wood). Of the foreign provenances, D 146 – Schwarzwald mit Baar, S 9 – Kriváò, and S 6 – Èierny Váh produced trees that showed above-average values. The smallest growth in height was found in the trees from the provenances BG 132 – Rila, Borovets, F 104 – Departement de µ Aude, I 228 – Vallombrosa, A 93 – Wörschachwald. Although some trees of Italian provenance achieve above-average volume, due to high mortality, they are not suitable for economic use in the forests of the Czech Republic. Based on the European zoning for forests, the units from the Central European region (3.11.0 – Èeské Polabí, 3.32.0 – Schwarzwald with foothills and Baar, and 9.14.0 – Southern Apennine Mountains) were the best rated in all parameters. Specimens from 9.12.0 – Mountain forest of the northern Apennines, and 4.05.0 – Vosges showed the lowest parameters. |
Harnessing nature's secrets: Silver nanoparticles from Withania coagulans fruit and root extracts unveil exceptional antioxidant and antimicrobial propertiesOriginal PaperFarwa Iftikhar, Rahmatullah Qureshi, Ayesha Siddiqa, Khursid Anwar, Fizza Arshad, Zia-ur-Rehman Mashwani, Aayesha Riaz, Safir Ullah Khan, Amir Ali, Shahzad Iqbal, Ajaz Ahmad, Melissa Danae Bejarano GómezCzech J. Food Sci., 2024, 42(3):192-206 | DOI: 10.17221/39/2024-CJFS Nanotechnology, an emerging field, holds significant promise with applications across diverse sectors, including medicine, agriculture, and the biological sciences. To address environmental concerns, the green biosynthesis of silver nanoparticles (AgNPs) using plant extracts is favoured. This study focuses on the formulation and characterisation of AgNPs using extracts from Withania coagulans (Stocks) Dunal, a medicinal plant that holds a unique phytochemical profile. The AgNPs derived from W. coagulans root (WcAgNPR) and fruit (WcAgNPF) extracts were characterised using ultraviolet and visible light (UV-Vis) spectral analysis, scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), and Fourier-transform infrared spectroscopy (FTIR) spectroscopy. The findings reveal that both WcAgNPR and WcAgNPF exhibit substantial antioxidant potential, with robust iron reducing capabilities and potent 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity. Additionally, they demonstrate strong hydrogen peroxide scavenging abilities. Notably, WcAgNPR outperforms WcAgNPF in the phosphomolybdate assay for antioxidant potential. Both AgNPs display remarkable antimicrobial efficacy, with minimal inhibitory concentrations (MIC) below 10 µg·mL–1 against gram-positive bacteria (Staphylococcus aureus) and noteworthy activity against gram-negative Escherichia coli (WcAgNPF with a MIC of 30 µg·mL–1 and WcAgNPR with a MIC of 60 µg·mL–1). These findings highlight the silver nanoparticles' significant antioxidant and antimicrobial potential, suggesting their potential for in vivo use as antimicrobial agents with minimal oxidative damage. |
Effects of cultivation duration of the crop and growth stages on rhizosphere soil physicochemical properties, enzyme activities, and microbial communities of ginseng under forestOriginal PaperYuchi Zhao, Qiuyu Wang, Shuaiqi Feng, Yang Zhang, Weiwei Dong, Wenxiu JiPlant Soil Environ., 2024, 70(9):562-579 | DOI: 10.17221/250/2024-PSE In this study, Illumina MiSeq sequencing of 16S and ITS2 rRNA genes were used to determine the dynamic changes in bacterial and fungal communities and soil properties and enzyme activities in rhizosphere soil of ginseng under forest after 5, 10 and 15 years of cultivation and different growth stages. Results showed that the changes were particularly prominent in 10-year-old ginseng under forest, and the trends of organic carbon, alkaline hydrolysed nitrogen, and available potassium were extremely similar in different duration of the crop, especially in the middle stage of rapid root growth, when soil nutrient consumption was severe, and soil enzyme activities of rhizosphere were significantly reduced. The observed changes in soil properties and enzyme activities caused by the cultivation duration of the crop and growth stage could be explained by the variations in the microbiome. The microbial composition of 10-year-old ginseng under forest has undergone significant changes, at the genus level, both Acinetobacter bacteria and Kazachstania fungi exhibited a higher abundance; the abundance of Bacillota (Firmicutes), and Candidatus udaeobacter with significantly lower abundance. This study initially revealed the changes in nutrient utilisation of ginseng under forest at different cultivation duration of the crop and different growth stages, as well as the regulatory role played by microbes in this process preliminarily. We consider 10 years to be a critical stage for the long-term cultivation of ginseng in the forest, during which it is more sensitive to environmental factors and may exhibit special dynamic changes affecting its growth and quality. This provides a reference for further precision planting and harvesting of ginseng under the forest. |
The effect of parameter adjustment in sago palm classification-based convolutional neural network (CNN) modelOriginal PaperSri Murniani Angelina Letsoin, David HerákRes. Agr. Eng., 2024, 70(3):123-133 | DOI: 10.17221/65/2023-RAE In our study location, Merauke Regency, the easternmost city in Indonesia, the sago palm is associated with different types of ecosystems and other non-sago vegetation. During the harvesting season, the white flowers blossoming between the leaves on the tops of palm trees may be distinguished manually. Four classes were determined to address the visual inspections involving different parameters that were examined through the metric evaluation and then analysed statistically. The computed Kruskal-Wallis test found that the parameters vary in each network with a P-value of 0.00341, with at least one class being higher than the others, i.e., non-sago with a P-value of 0.044 with respect to precision, recall, and F1-score. Thus, the general linear model (GLM) was tested specifically in trained Network-15 and Network-17, which have similar parameters except for the batch size. It indicated the two networks' differences based on their prediction results, classes, and actual images. Accordingly, a combination of learning rate (Lr) and batch size improved the reliability of the training and classification task. |
Did the COVID-19 pandemic disturb intra-EU trade in agrifood products? Evidence from a counterfactual forecasting approachOriginal PaperMariusz Hamulczuk, Karolina Pawlak, Daniel Sumner, Grzegorz SzafrañskiAgric. Econ. - Czech, 2024, 70(11):556-564 | DOI: 10.17221/253/2024-AGRICECON In this study, we attempt to infer the effect of the COVID-19 pandemic on the intra–European Union (EU) agrifood trade from out-of-sample forecasts. We compare the actual level of trade during the COVID-19 period with counterfactual values derived from univariate forecasting models [regARIMA (Linear regression with autoregressive integrated moving average errors) and Holt-Winters methods]. We analyse agrifood imports and exports of specific EU countries and the EU-27 aggregate on the basis of monthly data for the period from January 2010 to February 2022. The findings reveal a significant decrease in trade activity in the first year of the pandemic that was negatively correlated to COVID-19 restrictions applied by EU countries. Surprisingly, COVID-19 restrictions do not significantly explain the diversified agrifood trade response among EU countries during the pandemic. |
Effects of elevated CO2 and cadmium stress on vegetable quality and cadmium accumulationOriginal PaperXiao Wang, Deyan Li, Xiaohui SongHort. Sci. (Prague), 2024, 51(4):270-277 | DOI: 10.17221/131/2023-HORTSCI The aim of this study was to determine the effects of elevated CO₂ (eCO2) on the quality and cadmium (Cd) accumulation of vegetables grown in soil with a background Cd level or in Cd-contaminated soil. We used four types of vegetable: pak choi (Brassica rapa L.), water spinach (Ipomoea aquatica Forssk.), cherry radish (Raphanus raphanistrum subsp. sativus (L.) Domin) and pepper (Capsicum annuum L.). Cd stress significantly reduced vegetable vitamin C and reducing sugar content; however, under eCO2, vitamin C and reducing sugar content levels were significantly higher than they were under ambient CO2 (aCO2) levels. The nitrate content of pak choi, cherry radish and pepper was reduced under eCO2, and the effect was more pronounced among plants grown under Cd stress. The Cd content of cherry radish and pepper roots grown under eCO2 and Cd stress was significantly reduced (29.2% and 18.5%, respectively) compared with plants grown under aCO2 conditions and Cd stress. The Cd transfer coefficient of pak choi and water spinach grown under eCO2 and Cd stress was significantly lower (22.7% and 25.2%, respectively) than under aCO2 conditions. Our data suggest that growing vegetables under eCO2 is beneficial, especially when grown in Cd-contaminated soil, because Cd accumulation is reduced and vegetable quality is improved. |
Effects of biogas residue addition, as cultivation substrate, on ginseng growthOriginal PaperYan Gui, Meng Suo, Zhijie Qiu, Hao Wu, Zongjun Cui, Hongyan YangPlant Soil Environ., 2024, 70(2):101-110 | DOI: 10.17221/430/2023-PSE The effects of biogas residue as a substrate on ginseng growth and its feasibility for ginseng cultivation are unclear. The present study used biogas residue at different concentrations and maturity levels to cultivate ginseng. The biological characteristics of ginseng, soil physiochemical indices, and ginseng and soil microbial communities were investigated. The results showed that with increasing ginseng content and maturity, the total fresh weight, total length and saponin content significantly increased. The enzyme activities of soil, NO3–-N, and available phosphorus also increased. The microbiome analysis revealed that with the addition of biogas residue, microorganisms related to plant growth promotion, such as Chloroflexi, Gemmatimonadota and Mortierellomycota, were more common in the plant or rhizosphere soil. The results based on the co-occurrence network showed that the structure of the bacterial community was more stable than that of the fungal community with increasing biogas residue content. Our results indicated that biogas residue could be used as a ginseng cultivation substrate and promote growth. |
Cup viscometer – A practical analytical toolShort CommunicationMilan Hou¹kaCzech J. Food Sci., 2024, 42(1):71-76 | DOI: 10.17221/159/2023-CJFS
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Effects of combined application of animal slurry and mineral fertiliser on rice yield and soil nitrogen cycle microbesOriginal PaperPengcheng Wang, Siyuan Jin, Henglin Xiao, Zhi Zhang, Cheng Hu, Yan Qiao, Donghai Liu, Xifa Guo, Xiangrong PengPlant Soil Environ., 2024, 70(4):220-228 | DOI: 10.17221/315/2023-PSE This paper studied the response of rice yield and soil nitrogen (N) cycling microorganisms to the combined application of animal slurry and mineral fertiliser. A pot experiment was conducted on typical yellow-brown paddy soil. There were five treatments: (1) CK – no N fertilisation; (2) S0 – mineral fertilisation; (3) S30 – 30% slurry with 70% mineral fertilisation; (4) S60 – 60% slurry with 40% mineral fertilisation; and (5) S100 – slurry application. Rice yield, yield components, and soil properties were investigated at harvest. The abundance of soil N cycle functional genes abundance was quantified via quantitative real-time PCR. The rice yield reached a high level when the proportion of slurry used to replace mineral fertiliser was 30–50%. The yield in response to mineral fertiliser (S0) was equivalent to that in response to no N fertilisation since the formation of effective panicles was inhibited. With the slurry replacement ratio increase, the available phosphorus and potassium contents in the soil improved, but the nitrate content decreased. Considering the entire soil N cycle, nitrogen-fixing microbes (nifH), ammonia-oxidising archaea (AOA amoA) and nitrite-reducing microbes (nirS and nirK) had greater abundances, reaching 108 copies. Compared with those in the S0 treatment, the abundances of most N cycle functional genes in the S30 treatment, except for napA, significantly increased from 31.2% to 100.9%, and the increase in the abundance of nirS and nosZ in the S100 treatment reached 4 times, which was obviously greater than that of the other genes. Correlation analysis revealed that high soil pH promoted N fixation and nitrification, while NH4+-N had the opposite effect on N fixation and nitrification, and available phosphorus and potassium actively influenced denitrification. These results showed that a 30–50% slurry application ratio was recommended for rice, which was beneficial for maintaining high yields and high abundances of soil N cycle functional genes. |
Impact of selected antimitotic substances on doubled haploid and polyploid regeneration in microspore cultures of swede (Brassica napus ssp. napobrassica (L.) Hanelt)Original PaperMiroslav Klíma, Yamen H. Shmeit, Pavel Kopecký, Pavel Vítámvás, Klára Kosová, Ilja T. Prá¹il, Eloy Fernández-CusimamaniCzech J. Genet. Plant Breed., 2024, 60(2):79-85 | DOI: 10.17221/84/2023-CJGPB Several antimitotic agents were tested in three embryogenic doubled haploid (DH) lines of swede (Brassica napus ssp. napobrassica). No effect on embryogenesis was observed at the given concentrations of colchicine (5 μmol/L or 50 mg/L) and treatment time of 24 hours. Flow cytometric analysis of microspore embryos revealed a significant increase in the percentage of DH compared to the control for all substances: ethalfluralin, 62.2%; trifluralin, 58.3%; colchicine, 56.1%; amiprophos-methyl, 54.4%; pronamide, 35.0%; control, 13.3%. The occurrence of pure tetraploids was low (0–5%), mixoploids – especially n+2n – were high in all treatments, including the control (29.4–71.1%). Their presence can be explained as a consequence of spontaneous diploidisation and/or endoreduplication during embryogenesis or embryo germination. |
Information sources in agricultureOriginal PaperJan Jarolímek, Jakub Samek, Pavel ©imek, Michal Stoèes, Jiøí Vanìk, Jan PavlíkPlant Soil Environ., 2024, 70(11):712-718 | DOI: 10.17221/361/2024-PSE The aim of this study is to define data sources and propose methods for effective and secure data management in an agricultural enterprise in the context of using data for decision support. Current developments in information and communication technology (ICT) have contributed towards the increase in the amount of generated data in various fields. The main data sources for agricultural enterprises are the farm itself, suppliers, government, market, and research. The use of smart solutions, artificial intelligence, and other innovative practices in agriculture is discussed at many conferences, in various journals, strategies and project plans. Data is the essential raw material for all these solutions. Large amounts of data cannot be analysed efficiently with spreadsheet programs. Currently, there are trends in the use of data, for example, in business intelligence (decision-making systems), e.g. tools using online transaction processing (OLAP) or process automation or the possibility of e.g. tracing the origin of food. The availability and possibility of creating large data sets bring many challenges related to managing that data. To effectively manage farm data, it is essential to have a well-developed data management plan (DMP) used to formalise the processes related to handling. A DMP mainly addresses archiving, backup, licensing and other important aspects of data management. The challenges and developments in farm data management include incorporating artificial intelligence into data analysis and security. Food is classified as an "Entity of Critical Importance" in the NIS2 EU Directive, which also deals with cybersecurity issues. |
Application of biostimulation and bioventing system as bioremediation strategy for the treatment of crude oil contaminated soilsOriginal PaperIfeanyi Michael Smarte Anekwe, Yusuf Makarfi Isa*Soil & Water Res., 2024, 19(2):100-110 | DOI: 10.17221/66/2023-SWR The purpose of this study was to evaluate the application of biostimulation and bioventing for the treatment of crude oil-contaminated soils. The research needed to check how various industrial biostimulants will perform in the treatment of contaminated soils and whether or not there is a synergetic effect that has to do with the stimulant composition. Soil samples used for this study were collected from South Africa. The soil samples were air-dried for 24 h and subsequently passed through a standard sieve of 2 mm screen. The soil consists of 79.32% sand (2.00–0.02 mm), 14.71% silt (0.02–0.002 mm) and 5.97% clay (< 0.002 mm). A microcosm system containing 1 kg of soil contaminated with crude oil (5% w/w) for biostimulation (BSTc) treatment was amended with varying ratios of municipal wastewater (MWW) and brewery wastewater (BWW) to investigate the possible synergy. The bioventing (BVTc) treatment involves the supply of atmospheric air to the bioreactors through the vadose zone for 30mins flow duration every 48 h intervals at ambient condition for 28 days. The BSTc and BVTc treatments recorded 48–58% and 54–75% total petroleum hydrocarbon (TPH) removal efficiencies, respectively, as the BWW amendment noted appreciable removal compared to MWW, while the control treatment recorded 35%. The result showed that the attempt to boost the TPH removal efficiency using the bioventing with the wastewater amendment was effective, as the presence of enough oxygen in the system resuscitated the activities of the microbial community for enhanced TPH biodegradation. This study inferred that combined bioventing and biostimulation techniques proved to be an effective bioremediation strategy for the treatment of crude oil contaminated soils and could serve as a vital tool towards the mitigation of pollution aftermath faced by communities involved in oil production and/or processing activities. |
Are technological or efficiency differences more pronounced between Hungarian and Polish poultry farms? A stochastic metafrontier analysisOriginal PaperLajos Baráth, Imre Fertõ, Jakub StaniszewskiAgric. Econ. - Czech, 2024, 70(8):406-413 | DOI: 10.17221/322/2023-AGRICECON
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The sex ratio of spruce bark beetles – Ips typographus (Coleoptera: Curculionidae: Scolytinae) captured in different types of trapsOriginal PaperMarie Zahradníková, Jaroslav Dostál, Petr ZahradníkJ. For. Sci., 2024, 70(12):603-609 | DOI: 10.17221/55/2024-JFS In forest protection, various types of trap systems are used against spruce bark beetles to treat the foci of infested areas. Traditionally, these include pheromone traps, treated trap trees, and recently also the application of Storanet insecticide nets used for the sanitation of infested wood (trees), which also serve as treated trap trees when pheromones are applied, and the Trinet P system, a variation to a treated trap tree – a tripod. On an aluminium tripod, there is a Storanet insecticide net baited with a pheromone dispenser similar to other types of trap systems. The sex ratio proves to be a significant aspect in the assessment of individual types of trap systems. It is more favourable to catch a larger number of female individuals given that they bear the future development of the population. With a higher number of captured male individuals, the number of females per one male in the insect gallery increases, and the population cannot be reduced sufficiently. The results show that all three types of measures can be characterised by a lower ratio of captured males (approximately 40%) and a higher representation of females (around 60%). Differences in individual types of trap systems are insignificant. |
Prebiotic and antioxidant effects of the extracts from fruits and flowers of Cereus hildmannianusOriginal PaperEverton da Silva Santos, Gabriela Krausová, Ivana Hyr¹lová, Maria de Fátima Pires da Silva Machado, Arildo José Braz de Oliveira, Gizem Özlük, Regina Aparecida Correia GonçalvesCzech J. Food Sci., 2024, 42(6):415-422 | DOI: 10.17221/128/2024-CJFS
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Evaluation of certain pomological and phenological traits of selected asian pear varieties growing in Middle European conditionsOriginal PaperTomá¹ Neèas, Jan Wolf, Tomá¹ Kiss, Martina Göttingerová, Ivo Ondrá¹ek, Monika BieniaszHort. Sci. (Prague), 2020, 47(2):81-92 | DOI: 10.17221/83/2019-HORTSCI The cultivation and breeding of new pear tree varieties have great potential, especially with the changing climate. The introduction of genetic resources and varieties from regions with a high genetic diversity can have a positive effect on the cultivation of pear trees in our region. In this experiment, a total of 23 Asian pear varieties originating from Japan and China were assessed. Based on the values obtained, the varieties were sorted, and suitable varieties for the conditions of the Czech Republic were selected. The early-maturing varieties that proved to be promising were 'Shinseiki' and 'Zao Su Li' due to their refreshing juiciness, plasticity and ease of cultivation. generally low demand. The 'Shinseiki' bears medium- to small-sized fruits (103 g on average). The size of the fruits of the evaluated cultivar 'Zao Su Li' can reach very large sizes (255 g on average). The medium-maturity varieties suitable for the conditions of the Czech Republic were 'Hosui' and 'Dangshansu Li' (ripening on the September 11-19 on average) and another interesting variety, 'Kirgizkaja zimnaja'. Other suitable medium-maturity varieties are 'Nijisseiki', 'Sha Li' and 'Pung Su'. Of the assessed late-maturing varieties, the promising ones were 'Ya Li' and 'Mut Chen', especially due to their relatively high storability under cold storage. |
Values and professional and ethical norms in rural tourism in PolandOriginal PaperJan SIKORA, Wlodzimierz KACZOCHA, Agnieszka WARTECKA-WAZYNSKAAgric. Econ. - Czech, 2015, 61(8):377-392 | DOI: 10.17221/14/2014-AGRICECON With development of the market economy, the views concerning the importance of ethical norms in business activity are developing. Ethical orientation in economics, has been popularized for several decades by e.g. French centre "Economie et Humanisme"1, established by a renowned Dominican, L.J. Lebret. Tourism, including rural tourism, represents an economic activity which has its specific ethical dimension. The goals of this paper are to characterize the basic values that are voluntarily accepted and used in tourism in rural areas in Poland and to identify the ethical norms that guarantee the realization of these values. The authors adopted the basic values-goals that should be recommended in rural tourism. They also formulated specific professional and ethical norms that support realization of the values, which include: humanity and standards for realizing the principle of humanity; the cultural and material conditions of living in rural areas; economic activity, including tourist activity that improves rural living conditions; innovative attitudes towards modernization and improving the quality of rural tourist services; and values that support the development of sustainable tourism in rural areas. The theoretical investigations concerning axiological problems were supplemented with the results obtained from a national-level empirical survey that was conducted in rural areas in Poland. |
Impact of irrigation techniques on rice yield and dynamics of zinc in plants and soilOriginal PaperPhuong Dinh Thi Lan, Hoa Nguyen Thanh, Nga Nguyen Thi HangPlant Soil Environ., 2020, 66(3):135-142 | DOI: 10.17221/660/2019-PSE Zinc (Zn) insufficiency and water deficiency are primary challenges in intensive rice production systems. This study aims to examine the influence of two irrigation regimes, flood irrigation (FI) and water-saving irrigation (WSI), on rice grain yield and mobile Zn accumulation in soil and rice grains. Experiments were conducted in An Vien rice fields in the Tien Lu district, Hung Yen province, located in the middle of the Red River delta during four rice seasons from 2015 to 2016. The results showed that the WSI regime dramatically increased the grain yield and Zn concentrations in grain of rice. Grain yield was increased by 14.76% and grain Zn concentration by 17.93% when compared with the FI regime. The decrease in the mobile Zn concentration in soil was only 5.7% in the WSI technique, compared with 73.6% for FI techniques. Therefore, it can be concluded that WSI can be effective agricultural practice to elevate grain yield and increase Zn retention in soil and bioavailability in rice grains. |
Sorption isotherm modelling of dried tomatoesOriginal PaperAhmad Khalid Nayab, ¥ubomír Valík, Pavel AèaiCzech J. Food Sci., 2024, 42(1):21-30 | DOI: 10.17221/109/2023-CJFS
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Influence of the storage duration on the health promoting tyrosine, tryptophan, and total phenolics in potato tubersOriginal PaperRita Asakaviciute, Zita MaknickieneCzech J. Food Sci., 2024, 42(2):93-99 | DOI: 10.17221/161/2023-CJFS This research investigated the influence of the storage duration on the health promoting tyrosine, tryptophan and total phenolics in potato tubers. In the course of storage, the total amount of accumulated compounds in the dry mass of organically grown potato tubers increases. This is determined by individual properties of potato variety, storage time, and interaction of these two factors (P < 0.05). Organic potato tubers show increased total phenolic compounds in their dry matter during storage. This is due to a variety of characteristics, storage time and the interaction of these two factors (P < 0.05). A more pronounced increase in total phenolic compounds during storage was observed in 2022 than in 2021. |
The influence of timing and planting proportion on the intraspecific competitiveness ability of drunken horse grass (Achnatherum inebrians (Hance) Keng) by fungal endophyte infectionOriginal PaperJianxin Cao, Xiang Yao, Xuekai Wei, Ruochen Zhang, Jing Liu, Chunjie LiPlant Soil Environ., 2024, 70(7):418-429 | DOI: 10.17221/9/2024-PSE Drunken horse grass (Achnatherum inebrians (Hance) Keng) is a toxic perennial bunchgrass native to Northwestern China. Epichloë endophytic fungi infection could enhance the stress tolerance of drunken horse grass. However, there is a scarcity of literature regarding the effects of intraspecific competition. As a result, we anticipated that the intraspecific competitive dynamics between endophyte-infected (EI) and endophyte-free (EF) plants would become more transparent for four years by planted as the proportions of 2 : 1, 1 : 1, and 1 : 2. The results showed the EI plants exhibited more biomass, seed yields, and survival rates than EF plants. Endophyte infection also facilitated a competitive advantage by enhancing photosynthesis and soil nutrition. Our findings constituted the inaugural investigation into the influence of the intraspecific competitive ability of grass infected with Epichloë endophyte fungi. EI plants caused them to become stronger and stronger, while EF became weaker and weaker by timing and planting proportion increasing, and EF drunken horse grass could be replaced by EI. These conclusions were instrumental in elucidating why the endophytic fungal infection rate of drunken horse grass is 100% observed in natural wilderness. Epichloë endophyte could reduce plant diversity and enhance the dominance of EI plants in intraspecific competition; drunken horse grass may be threatening the persistence of native plant species. |
Impact of parent inbred lines on heterosis expression for agronomic characteristics in sunflowerOriginal PaperAssadollah Zakeri Haddadan, Mehdi Ghaffari, Eslam Majidi Hervan, Bahram AlizadehCzech J. Genet. Plant Breed., 2020, 56(3):123-132 | DOI: 10.17221/100/2019-CJGPB To study the impact of parent-inbred lines on the heterosis expression of the agronomic characteristics in sunflower hybrids, 24 sunflower hybrids along with the parent lines were evaluated for their agronomic characteristics as a randomised complete block design with three replications in the 2018-2019 growing seasons in Karaj, Iran. According to the results, the hybrids R29 × A346, R19 × A346, R29 × A40 had the highest achene yield (4 159, 4 143 and 4 108 kg/ha, respectively), but the highest heterosis was observed in R29 × A212 and R19 × A212 (182 and 181%, respectively) suggesting that the incidence of heterosis is related to the relative performance of both the parents and hybrids. The results confirmed the heterosis expression for most of the agronomic traits. The heterosis for the days to flowering and maturity were negative. All the mid-parent heterosis (MPH) for the plant height, head diameter, stem diameter and achene number were positive, while only the plant height was positive for the best parent heterosis (BPH). Almost all the MPH and BPH of the crosses for the achene and oil yield were positive, which indicates a considerable heterosis for the achene and oil yield. The results showed that the relative impact of the restorer (R)-lines was higher than the cytoplasmic mail sterile (CMS)-lines on the heterosis expression for the days to maturity, stem diameter, achene number per head and achene and oil yield. The CMS-lines had more of an impact on the heterosis expression for the plant height and the relative impact of the R-lines and CMS-lines were almost similar for the days to flowering, head diameter, achene weight and oil content. Due to the higher relative impact of the paternal lines on the heterosis expression for half of the studied characteristics in this study, choosing suitable parental lines will have a crucial role in breeding the sunflowers for a desired trait. |
Do peers and agglomeration affect farm efficiency?Original PaperSunhyung Min, Kwansoo KimAgric. Econ. - Czech, 2024, 70(8):395-405 | DOI: 10.17221/99/2024-AGRICECON
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Prohexadione calcium regulates wheat tolerance to drought stress by maintaining water balance and promoting antioxidant metabolism and photosynthesisShort CommunicationZiyang ZhangPlant Soil Environ., 2024, 70(10):673-681 | DOI: 10.17221/247/2024-PSE This study explored whether and how prohexadione calcium (Pro-Ca) regulated wheat tolerance to drought stress (DS). Findings displayed that DS had significant influence on antioxidant metabolism, water balance and the photosynthesis. DS significantly improved the activity level of enzymatic antioxidants superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and ascorbate peroxidase (APX), the contents of non-enzymatic antioxidants ascorbic acid (AsA) and glutathione (GSH), electrolyte leakage (EL), malondialdehyde (MDA), and the contents of osmotic regulatory substances soluble protein (SP), soluble sugars (SS) and proline (Pro), compared with control. Whereas DS significantly reduced transpiration rate (Tr), stomatal conductance (gs) and relative water content (RWC), photosynthetic pigments chlorophyll and carotenoid contents, net photosynthetic rate (Pn), maximum photochemical efficiency of PSII (Fv/Fm), plant height and biomass. Compared to DS, Pro-Ca plus DS significantly promoted the antioxidant metabolism by improving the activity level of SOD, CAT, POD and APX and increasing AsA and GSH contents, which in turn reduced MDA content and EL. In addition, Pro-Ca plus DS significantly maintained water balance by promoting the accumulation of osmolytes SP, SS and Pro, which in turn increased RWC, Tr and gs. Pro-Ca plus DS also significantly promoted photosynthesis by increasing the contents of the above photosynthetic pigments, Pn and Fv/Fm, thereby promoting plant growth. These findings indicated that Pro-Ca was a potential agent to improve wheat tolerance under water deficit. |
Does a relation between bone histomorphometry and fractures exist? The case of the equine radius and tibiaOriginal PaperM Zedda, R Babosova, S Gadau, G Lepore, S Succu, V FarinaVet Med - Czech, 2024, 69(9):307-313 | DOI: 10.17221/18/2024-VETMED Fractures of long bones in limbs are rare traumatic events in horses. This study investigates whether the incidence and types of fractures can be related to the histomorphometric features of the radius and tibia, which experience different biomechanical stresses and exhibit varying incidences and types of fractures. Clinical observations suggest that, in adults, slightly transverse and comminuted fractures are present in the radius, while the tibia shows a higher frequency of longitudinal and spiral fractures. Microscopic observations reveal no apparent distinctive characteristics between the radius and tibia, whereas the histomorphometric data highlight differences in the osteon density, eccentricity, and diameters of the osteons and Haversian canals. To sum up, tibial osteons are more numerous and smaller than those in the radius, resulting in a 15% higher total extension of the cement line in the tibia compared to the radius. These histomorphometric differences are an evolutionary adaptation to the different biomechanical stresses that involve the thoracic and pelvic limbs. Our results could help better understand numerous clinical realities detectable through retrospective analyses and aid in evaluating a specific bone’s predisposition towards traumatic events in all mammals, including humans. |
Evaluation of chemical compounds as repellents of the brown marmorated stink bug (Halyomorpha halys [Stål])Original PaperZiga Laznik, Matej Podgornik Milosavljeviæ, Stanislav TrdanPlant Protect. Sci., 2024, 60(4):371-379 | DOI: 10.17221/87/2024-PPS The brown marmorated stink bug [Halyomorpha halys (Stål)] is an invasive pest species. This polyphagous insect, native to Eastern Asia, threatens various cultivated plant species. Control methods often rely on chemical insecticides, but the decreasing use of such agents has prompted a shift towards preventive measures. As a defence mechanism, natural compounds released by plants have gained attention for their potential deterrent or attractant properties. In this study, we evaluated the response of the brown marmorated stink bug to selected chemical substances, including citronellal, hexanal, nonanol, β-caryophyllene, linalool, ocimene, nerolidol, terpinolen, α-humulene, dimethyl sulfide, aggregation pheromone, and ethanol. The experiment was conducted using an olfactometer. Two experiments were performed, comparing the substances against dimethyl sulfoxide and then refining the selection based on initial results; in the first series, nerolidol, ocimene, and terpinolene exhibited promising results as complete deterrents. The second series confirmed nerolidol as the most effective deterrent among all tested substances. These findings contribute to developing preventive strategies for managing the brown marmorated stink bug and reducing reliance on chemical insecticides. |
Ambient background and quality reference values for trace metals in soils from AlgeriaOriginal PaperAbdelkader Laribi, Charles Shand, Renate Wendler, Brahim Mouhouche, Stephen Hillier, Gilles ColinetSoil & Water Res., 2023, 18(1):33-42 | DOI: 10.17221/143/2021-SWR The establishment of the reference ambient background concentrations (ABCs) and quality reference values (QRVs) for trace metal (TM) concentrations in soils are required for the environmental assessment and any implementation of a protective action. This information is lacking for soils of the eastern Mitidja plain, which is an important agricultural production area in Algeria. Data for the aqua regia extractable Cd, Cr, Cu, Fe, Ni, Pb and Zn concentrations from 180 composite topsoil samples taken across the Mitidja plain in a stratified random pattern were statistically analysed. Descriptive statistical methods and linear regression equations were applied to determine the upper limit of the ABCs for the TMs. After removal of outliers, the derived QRVs were: Cd 0.24, Cr 62.1, Cu 99.3, Fe 45 590, Ni 47.7, Pb 33 and Zn 115 mg/kg. Iron is a macro element in the soils, but is included as its concentration can be used to normalise the concentrations of the other elements. The derived QRVs are similar or less than those reported for other regions of the world, apart from Cu, where a wide range (36 to 206 mg/kg) is reported. These reference values can be used to identify areas that may require follow-up surveys or to identify priority sites for decision making. |
Investigation of multimycotoxins by LC-MS/MS in maise semolina chipsOriginal PaperOktay Samadzade, Karlo Muratoğlu, Serkan Kemal BüyükünalCzech J. Food Sci., 2023, 41(3):212-220 | DOI: 10.17221/241/2022-CJFS
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Genetic diversity and maternal origin of Vietnamese indigenous chicken breeds inferred from complete sequences of mitochondrial DNA D-loop regionOriginal PaperThi Thanh Nhan Giang, Van Ba Nguyen, Thi Quynh Chau Nguyen, Khanh Van Nguyen, Lan Doan PhamCzech J. Anim. Sci., 2023, 68(10):423-432 | DOI: 10.17221/226/2022-CJAS Indigenous chickens play a significant role in food security, income growth and socio-cultural life of rural households in Vietnam. This study was conducted to assess the genetic diversity as well as the phylogenetic relationships of Vietnamese indigenous chickens (Gallus gallus) to support the conservation of their genetic resources for sustainable rural farming. In this study, the genetic diversity and the phylogenetic relationships of 10 Vietnamese indigenous chicken breeds were analyzed using complete sequences of mitochondrial DNA (mtDNA) displacement-loop (D-loop) region. The average nucleotide and haplotype diversities of Vietnamese chickens were 0.006 3 ± 0.000 22 and 0.918 ± 0.010, respectively. A total of 39 polymorphic sites and 29 haplotypes were identified. The maximum likelihood tree classified these haplotypes into seven haplogroups (A, B, C, D, E, G and V), with haplogroups A and B being the two predominant maternal lineages of Vietnamese indigenous chickens, while haplogroups C, D, E, G and V were found in the remaining chickens. Several haplotypes from different haplogroups were shared among some chicken breeds. These results suggested that Vietnamese indigenous chicken breeds have multiple maternal origins, mainly from Chinese, Southeast Asian and Indian chickens, and that these breeds share common maternal lineages. The high level of genetic diversity in Vietnamese chickens demonstrates significance of conservation for future use. |
Myxofibrosarcoma of the penis in an African pygmy hedgehog (Atelerix albiventris) – A clinical caseCase ReportL Kasalova, H Cernochova, R Dvorakova, A Angelova, Z KnotekVet Med - Czech, 2024, 69(4):130-135 | DOI: 10.17221/107/2023-VETMED
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