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Bovine respiratory disease (BRD) develops from complex interactions among environmental, host and pathogenic factors. This study aimed to phenotypically identify Enterobacter hormaechei isolated from cattle with BRD and assess antimicrobial susceptibility and determining the molecular phylogeny of local E. hormaechei strains. Between November 2023 and March 2024, nasal swabs were collected from 93 cattle with BRD, before culturing for phenotypic analysis, and performing the polymerase chain reaction (PCR) for molecular characterisation. Of the 93 samples evaluated, 15.79% and 24.56% tested positive for E. hormaechei isolates on culture and PCR, respectively. The local isolates exhibited high resistance to amoxicillin, ampicillin, amikacin, nalidixic acid and ceftazidime; high susceptibility to azithromycin, levofloxacin, gentamicin, ofloxacin, cefepime, ceftriaxone, cefotaxime, nitrofurantoin, ceftazidime and ciprofloxacin; and moderate susceptibility to ciprofloxacin, colistin, imipenem and meropenem. Multiple sequence alignment, phylogenetic tree analysis and homology sequence identification, showed that the five positive isolates were similar to the reference isolate. To the best of our knowledge, this is the first time that E. hormaechei has been isolated in cattle with BRD in Iraq. Because phenotype-based assays show limited accuracy to identify species, we recommend molecular and phylogenetic analysis be included in all similar studies in the future.

Two different morphologies of nanocellulose – cellulose nanofibres (CNFs) and cellulose nanocrystals (CNCs) with different contents were added to emulsified sausages to replace pork back fat in different proportions (10%, 30%, and 50%), and the colour, emulsion stability, texture characteristics and dynamic rheological behaviour of emulsified sausages were analysed. The results indicate substituting fat with nanocellulose led the L* (lightness) and a* (redness) values increased and the b* (yellowness) values decreased of the emulsified sausages. Increasing nanocellulose concentration improved the emulsion stability of the sausages. In terms of emulsion stability, the use of CNFs outperformed CNCs, especially for samples substituted with 50% fat. In terms of texture, emulsified sausages where CNCs replaced fat showed lower hardness, viscosity, and chewiness compared to those with CNFs. The dynamic rheological results indicated that when the fat replacement levels were 10% and 30%, samples using CNFs as fat substitute had slightly higher storage modulus (G') values than those using CNCs. However, for 50% fat replacement, samples using CNFs as fat substitute had significantly higher G' values than those using CNCs, which demonstrates that higher CNFs concentrations are more conducive to the formation of a three-dimensional network structure.

Sorghum (Sorghum bicolor L.) is one of the most important cereals in the world; is an important source of bioactive compounds. The germination is a very useful tool to improve the nutraceutical value of cereals, associated with the reduction of chronic-degenerative diseases; the extrusion has a positive effect on microbiological stability and sensory properties. The response of the combined germination-extrusion processes applied under optimised conditions, on proximal composition, in vitro protein digestibility (IVPD), total phenolic compounds (TPC), antioxidant activity (AoxA), hypoglycemic potential and microbiological quality of sorghum grains were studied. Sorghum was processed by germination (37 °C for 69 h) and extrusion [137 °C for 134 rpm (revolutions per minute)]. The germination increased protein content (+21%), insoluble dietary fibre (+50%), IVPD (+10%), TPC (+26%), AoxA (+97%). The extrusion increased soluble dietary fibre (+100%) and IVPD (+13%). The combined germination-extrusion processing reduced the content of total coliforms, total mesophilic aerobics and molds below the maximum limits established by the Mexican Official Standards NOM-147-SSA1-1994. Regarding hypoglycemic potential, germinated sorghum and germinated-extruded sorghum presented the best half maximal inhibitory concentration (IC₅₀) value. The combination of germination-extrusion processes is an effective strategy to increase bioactive compounds with antioxidant activity and inhibition of α-amylase and α-glucosidase enzymes.

The display of the depth of operation on tractors' dashboards facilitates the operator in achieving precise operation. In this study, the depth of operation of a mounted implement was measured and digitally displayed on a tractor's dashboard. The change in depth of operation was sensed by measuring the rotation of the rocker arm of the tractor's hydraulic system. The measured angle of rotation was multiplied by a calibration factor to convert it into the actual depth of operation in centimetres. For the calibration factor, a geometrical analysis of the three-point linkage was carried out, and a mathematical relationship was established based on the length of various linkages and their locations. A computer program was also developed to solve these equations to calculate the calibration factor. The program was validated with six different sizes of three-point linkages and found the maximum root mean square error was within 5%. The developed digital display was evaluated in the laboratory with three different implements and found a maximum error of ± 1 cm. A further evaluation was also carried out in an actual field with implements at three different depth levels, and a deviation of up to ± 13% was found with respect to the manual depth measurement.

The objective of this study was to compare the effects of calf starters of different starch and fibre content on the growth, blood and rumen parameters of Holstein female calves during the pre-weaning period. A total of 60 calves were divided into two groups. Group A/B was fed according to a two-phase calf nutrition system: the calves received calf starter "A" (28.5% starch content, 16% neutral detergent fibre (NDF), 9.2% acid detergent fibre (ADF)) in days 7-45 and then calf starter "B" (14.2% starch content, 32.4% NDF, 14.3% ADF) in days 46-70. Calves of group B received calf starter "B" throughout the experimental period. The calves were weaned between 52 and 61 days of life. The data on body weight, weight gain, starter feed intake, blood metabolites and rumen fermentation parameters were collected individually. In the first phase of experiment, the starter feed consumption of A/B group was higher than that of B group (P < 0.05), but it did not manifest in differences in body weight at weaning time. At some of the sampling times, the different composition of starter diets significantly (P < 0.05) influenced the plasma urea, albumin, glucose and triglyceride concentrations. The consumption of diet with different starch and NDF content had no significant effect either on the composition of the rumen microbiota or on the ruminal concentration of volatile fatty acids (VFA) and ammonia. In some cases significant correlations (-0.83 and -0.93; 0.82 and 0.90) were found between the composition of rumen microbiota and ruminal VFA concentration. Feeding a diet of higher starch content (28.5 vs 14.2%) in the first 45 days of life did not affect rumen fermentation, rumen microbiota and the most important blood metabolites, apart from a few exceptions. The production parameters of calves, including total feed intake and body weight gain, were similar in the experiment.

The effect of a brief exposure to treatments of end-of-day (EOD) far-red (FR) light and EOD-heating on flowering and stem elongation in certain species cultivated in areas with limited sunshine from autumn to winter has been investigated. Thirteen EOD-FR treated cultivars among all 24 cultivars tested in experiment 1, showed earlier flowering than control plants. Additionally, Kanzaki No.21, F1 Winter cherry and Gypsy deep rose were earlier to flower under the all night-FR treatment than under the EOD-FR treatment. Further, 13 cultivars among all cultivars tested in experiment 1 showed greater stem length, whereas 16 cultivars of them showed greater mean internode length than controls when treated with EOD-FR. The days to flowering in 13 cultivars treated by EOD-Heating, flowering was earlier than in controls. Also, stem length and mean internode length were also promoted by the same treatment in 7 cultivars. Additionally, flowering and stem elongation of Stella rose, Arizona sun, Suzuhime and Extra carmine rose were promoted by a combination of both treatments, and productivity and quality were improved. These results suggest that the treatments tested can help the production of cut-flowers in areas with limited sunshine from autumn to winter, although the method of application of the treatments must be optimized for each species.

Water and nutrient use efficiencies are important adaptive features of plants in arid and semi-arid environments. In this study, water, nitrogen (N), and phosphorus (P) use efficiencies of Stipa purpurea Griesb., an endemic and dominant grass species, were investigated in the alpine steppe along precipitation gradients on the Tibetan Plateau. The leaf N content of S. purpurea increased along the precipitation gradient, but leaf P decreased, whereas carbon (C) remained unchanged. Leaf δ¹³C (water use efficiency, WUE) and C : N ratio (N use efficiency, NUE) decreased with increasing precipitation. But leaf C : P (P use efficiency, PUE) and N : P ratios increased with increasing precipitation. A trade-off was found between WUE and PUE but not between WUE and NUE. The changes in leaf traits were associated with changes in soil water, organic C, total N and P. These findings offer insights into understanding alpine plant water and nutrient use strategies along a precipitation gradient, as well as facilitate the prediction of alpine ecosystem responses to precipitation changes.

Zinc (Zn) insufficiency and water deficiency are primary challenges in intensive rice production systems. This study aims to examine the influence of two irrigation regimes, flood irrigation (FI) and water-saving irrigation (WSI), on rice grain yield and mobile Zn accumulation in soil and rice grains. Experiments were conducted in An Vien rice fields in the Tien Lu district, Hung Yen province, located in the middle of the Red River delta during four rice seasons from 2015 to 2016. The results showed that the WSI regime dramatically increased the grain yield and Zn concentrations in grain of rice. Grain yield was increased by 14.76% and grain Zn concentration by 17.93% when compared with the FI regime. The decrease in the mobile Zn concentration in soil was only 5.7% in the WSI technique, compared with 73.6% for FI techniques. Therefore, it can be concluded that WSI can be effective agricultural practice to elevate grain yield and increase Zn retention in soil and bioavailability in rice grains.

Multiple primary malignant tumours (MPMTs) are multiple neoplasms with independent pathogenetic origins, placing great importance on the tumorigenesis and clinical treatment. However, due to the rare occurrence and diagnostic confusion, MPMTs have rarely been investigated in veterinary medicine. In this report, a 10-year-old intact female Maltese dog had MPMTs, consisting of two malignant tumours and one benign tumour each derived from a topographically different site: tubular carcinoma in the mammary glands, leiomyosarcoma in the uterus and sebaceous epithelioma in the cheek. The unique combination of MPMTs would be the first case in veterinary research to give insight into the diagnosis, disease characteristics, and surgical treatment.

Global climate change leads to frequent occurrence of low-temperature stress (LTS), which poses a serious threat to global food security. Here, environment-control phytotron experiments were conducted on cold-responsive cv. XM26 and cold-tolerant cv. YN19 during the anther differentiation period. Six LTS levels (4, 2, 0, −2, −4, −6 °C) and a control treatment (10 °C) were set to study the effects of different levels of LTS on wheat seed-setting characteristics and yield. LTS significantly decreased grain number per spike, 1 000-grain weight, and grain yield per plant (GYPP) of the two wheat cultivars. Each spike’s grain number and weight distribution showed a quadratic curve, and the near-medium dominance of grain development was not affected by temperature. The grain number percentage and grain weight of wheat at different grain positions were G2 (2^nd grain position) ≥ G1 (1^st grain position) > G3 (3^rd grain position) > G4 (4^th grain position), in which G3 and G4 grain positions were more sensitive to LTS. In summary, LTS during the anther differentiation in wheat mainly led to a decrease in GYPP by significantly reducing the number and weight of inferior grains. Improving wheat cultivation measures and promoting the development of inferior grains are significant ways to prevent disasters and increase wheat quality and productivity in the future.

In this paper, desorption isotherms of two jerky products were studied (whole-muscle jerky – sample 1 and minced jerky – sample 2). The work focused on the comparison of the Dynamic Dewpoint Isotherm (DDI) method and the Saturated Salt Slurry (SSS) method and testing the newly applied model for modelling desorption isotherms for dried meat. Data were statistically processed using 8 models [Guggenheim-Anderson-de Boer (GAB), Double Log Polynomial (DLP), Henderson, Chin, Smith, Oswin, Hasley, and newly applied model] and statistically evaluated using coefficient of determination (R²), root mean squared error (RMSE) and mean relative percentage deviation (P-value). The DLP model (25 °C) reached R² ≥ 0.999, P-value ≤ 1.84 for DDI, and R² ≥ 0.998, P-value ≤ 4.37 for SSS method. The GAB model reached R² ≥ 0.997, P-value ≤ 2.58 for DDI, and for SSS method the GAB model reached R² ≥ 0.998, P-value ≤ 5.47. The new model reached P-value ≤ 5.73 for DDI and P-value ≤ 3.48 for SSS method. All models reached the P-value < 10% except for Smith and Chin models. The DDI method and newly applied model prove to be a suitable and precise approach to the evaluation of isotherms of dried meat products.

Low water availability is a significant constraint on global crop production. Exploration is needed regarding plant responses to drought in interaction with biochar, encompassing optimised water use and carbon allocation strategies. The size of the biochar particles also plays an important role, especially in influencing the dynamics of water and plant growth. This study explored the potential impact of biochar treatment on radish growth and drought tolerance. Finer biochar particles lead to the most substantial available water content for plants, increasing at around 30%, while medium and larger fractions increase by about 22% and 16%, respectively, compared to control soil. The chlorophyll fluorescence technique showed improved water management of drought stress at larger fractions of biochar. Our research underscores the potential of biochar treatments for environmental stresses and water scarcity in modern agriculture.

Determining gallic acid in honey can provide information for assessing the nutritional value and tracing the source of honey. However, the complex matrix of honey and the low content of gallic acid may hamper the detection. Therefore, it is important to select an appropriate sample preparation method. This work established an air-assisted dispersive micro-solid phase extraction combined with a high-performance liquid chromatography method to determine gallic acid in honey. Zinc/nickel/aluminium layered double hydroxides were selected as the adsorbent to extract gallic acids in diluted honey samples. Under air-assisted extraction, the adsorbents adsorbed gallic acid in honey via anion exchange. Subsequently, the isolated adsorbents were dissolved in a 1% phosphoric acid solution. A high-performance liquid chromatography-UV-Vis detector was used for gallic acid detection. Under the optimised conditions, gallic acid showed good linearity over the concentration range of 0.005–10.0 mg·L^–1 with a coefficient of determination greater than 0.999. The detection limit and quantification limit were 13.5 and 45 ng·g^–1, respectively. The recoveries were 89.8–93.4%, with the intra-day and inter-day relative standard deviations in the range of 0.71–1.17% and 0.76–1.27%, respectively. The method possesses the advantages of simplicity, rapidity, economy and environmental friendliness and is suitable for detecting gallic acid in honey.

Porang (Amorphophallus muelleri) is  an Indonesian parental plant tuber developed vegetatively from bulbils during dormancy and harvested through petiole detachment for the industrial production of glucomannan. Pathogenic fungi and whiteflies can cause infection during harvesting and storage, destructing plant cells as well as reducing seed quality and crop yields. Therefore, this study aimed to develop a calibration model for detecting infected and non-infected porang bulbils using a computer vision system. Image parameters such as colour (red, green, blue – RGB and hue, saturation, intensity – HSI), texture (contrast, homogeneity, correlation, energy, and entropy), and dimensions (width, area, and height) were evaluated on 90 samples in three positions. The results showed that the majority of image quality properties were significantly associated with non–infected and infected porang bulbils as showed by Pearson correlation values of 0.901 and 0.943, respectively. Discriminant analysis based on image attributes effectively classified non-infected and infected seedlings, achieving a model accuracy of 97.0% for correctly classified cross-validated grouped cases. Therefore, computer vision can be used for the preliminary detection of fungal infection in porang bulbils, as evidenced by its high accuracy and outstanding model performance.

Common ragweed – Ambrosia artemisiifolia L. (Asteraceae) is an invasive plant species in Europe native to North America. Most of the records of known eriophyid mites on different ragweed species are from their native range. Our field experiments in Slovakia, 2016–2023, aimed to identify specific species feeding on common ragweed. We searched for symptomatic plants and collected growing tips, which were then preserved in 70% ethanol for further study. A recently described species of eriophyid mite, Aceria artemisiifoliae Vidović & Petanović (Acari: Eriophyoidea), was found in western and eastern Slovakia. This is the first record of the species in Slovakia and the second record in the world. It remains unclear whether this species is invasive like Ambrosia, and whether it could be used as a potential biological control agent.

This experiment aimed to evaluate the effects of chitosan administration to total mixed ration (TMR) silage on ruminal fermentation characteristics and degradability by using both in vitro and in sacco methods. The completely randomized design (CRD) used in this study included five treatments and six replications. The treatments consisted of TMR silage with distilled water as control (SA), TMR with 1% acetic acid (SB), 0.5% chitosan in 1% acetic acid diluent (SC), 1% chitosan in 1% acetic acid diluent (SD), and 1.5% chitosan in 1% acetic acid diluent (SE). The variables observed were ruminal fermentation parameters, methane production and in sacco nutrient degradability. Results revealed that adding 1–1.5% chitosan (SD and SE treatments) reduced (P < 0.05) total protozoa, total gas production, methane production, the percentage of acetic acid, and the ratio of acetic acid to propionic acid, while it increased (P < 0.05) the proportion of propionic acid. The addition of chitosan (SE treatment) decreased (P < 0.05) the degradation of dry matter, but it increased (P < 0.05) the degradability of organic matter and crude protein, as determined by in sacco analysis.

In Thailand, vibration problems often occur with rice combine harvester automatic header height adjusting systems. This study aimed to identify parameters for reducing the vibration and managing response time for harvesting speed configuration. An experimental combine harvester header model was designed to automatically adjust three parameters: total movement time, time ratio, and final phase distance within vertical movement ranges of 200, 250, or 300 mm. These parameters were controlled using a proportional flow control valve and a professional learning community (PLC) control unit. The results showed that increased time ratio, final phase distance, and total movement time significantly reduced average vibration amplitude. Higher time ratios corresponded to lower vibration amplitude during changing stages but higher amplitude during stopping stages. Vibration amplitudes during starting, changing, and stopping stages ranged from 0.622 to 1.373 mm, 0.042 to 1.097 mm, and 0.132 to 0.902 mm, respectively, for 200, 250, and 300 mm vertical movement distances. To reduce vibration in the first and second wave phases, precise control of start and final speeds through time ratio and total movement time was necessary. Minimizing the time ratio and final phase distance effectively reduced vibration amplitude in the third wave phase.

o uncover the regulatory metabolism of poly-glutamic acid (PGA) in protecting wheat crops against salt stress (SS) at the physiological level, we utilised hydroponic experiments to explore the roles of PGA in regulating the photosynthetic performance, water physiology, antioxidant metabolism and ion homeostasis of wheat seedlings exposed to SS for 10 days. The findings demonstrated that SS inhibited the photosynthetic performance of wheat seedlings. In contrast, different doses of PGA all improved the photosynthetic performance, especially for 0.3% PGA. Compared with SS, 0.3% PGA plus SS decreased nonphotochemical quenching (q_N) by 26.3% and respectively increased photosynthetic rate (Pn), soil and plant analyser development (SPAD) value, maximum photochemical efficiency of photosystem II (PSII) (F_v/F_m), photochemical quenching (q_P) and actual photochemical efficiency of PSII (Y(II)) by 54.0, 27.8, 34.6, 42.4 and 25.8%. For water metabolism, SS destroyed the water balance of wheat seedlings. In contrast, different doses of PGA enhanced water balance, especially for 0.3% PGA. Compared with SS, 0.3% PGA plus SS decreased leaf water saturation deficit (LWSD) by 35.5% and respectively increased leaf relative water content (LRWC), transpiration rate (T_r), stomatal conductance (g_s) and the contents of soluble sugars (SSS) and proline (Pro) by 15.9, 94.7, 37.5, 44.6 and 62.3%. For antioxidant metabolism, SS induced the peroxide damage to wheat seedlings. In contrast, different doses of PGA all mitigated the SS-induced peroxide damage, especially for 0.3% PGA. Compared with SS, 0.3% PGA plus SS respectively decreased superoxide anion (O₂^–), hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) contents and electrolyte leakage (EL) by 39.1, 29.6, 46.2 and 36.3%, and respectively increased superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR), dehydroascorbate reductases (DHAR) and monodehydroascorbate reductase (MDHAR) activities, and antioxidants ascorbic acid (AsA) and glutathione (GSH) contents by 69.2, 49.2, 77.8, 80.6, 109.5, 121.7, 104.5, 63.8 and 39.6%. Besides, SS destroyed the ion homeostasis of wheat seedlings. In contrast, different doses of PGA all maintained ion homeostasis, especially for 0.3% PGA. Compared with SS, 0.3% PGA plus SS reduced Na+ content by 40.7% and respectively increased K⁺, Ca²⁺ and Mg²⁺ contents by 64.4, 82.6 and 105.6%, thereby respectively increasing K⁺/Na⁺, Ca²⁺/Na⁺ and Mg²⁺/Na⁺ ratios by 177.6, 209.4 and 244.8%. In the above ways, SS inhibited wheat height and biomass. In contrast, different doses of PGA all improved wheat height and biomass under SS, especially for 0.3% PGA. Compared with SS, 0.3% PGA plus SS, respectively, increased wheat height and biomass by 27.4% and 41.7%. In the above ways, PGA mitigated salt toxicity in wheat seedlings. The current findings implied that there was a potential for the use of PGA in real situations to improve wheat salt tolerance, especially for the 0.3% dose.

Surgical site infections (SSIs) cause significant complications in the postoperative period in veterinary surgeries. Determining the aetiology of infections is crucial for increasing treatment success rates. This study aims to assess treatment processes by identifying the bacterial agents responsible for infections occurring in cats and dogs after orthopaedic operations and to determine the antimicrobial resistance profiles of these agents. Strains isolated from SSIs were retrospectively analysed in patients brought to the Surgical Clinic of Siirt University Animal Health Application and Research Hospital between 2021 and 2023. The isolates were identified using MALDI-TOF MS. The disc diffusion method was applied to determine the antimicrobial susceptibility of the isolates. A high isolation rate was detected in methicillin-resistant Staphylococcus pseudintermedius and extended-spectrum beta-lactamase (ESBL) producing Enterobacteriaceae. According to the antimicrobial susceptibility results, cephalosporin treatment was continued in only one of the cases in which prophylactic treatment with cephalosporin group antibiotics was applied. Consequently, this study revealed that preoperative prophylactic antibiotic administration may not be sufficient in preventing surgical site infections. Diagnosis of aetiological agents and evaluation of antimicrobial susceptibility are essential in preventing surgical site infections and determining effective treatment options.

This article aims to highlight the importance of climate and environmental challenges for agricultural economics and policy. Empirical research based on the Data Envelopment Analysis (DEA) method determined the average technical efficiency and scale efficiency of farms in the European Union in total and in economic size classes in the period 2004–2020. The results indicate that agriculture is generally characterised by high technical efficiency and scale efficiency. Analysis by economic size classes of farms, defined by the standard sum of their agricultural output, shows that the relationship between the scale of production and technical efficiency of farms is U-shaped. The highest technical efficiency and scale efficiency are characterised by small, large and very large farms economic classes. Economies of scale shift the burden of food production to large farms, which provide food security and are technically efficient but excessively burdensome for the climate and the environment. Small farms produce environmentally friendly food but on a small scale. Therefore, increasing the technical efficiency of medium-sized farms can contribute to more sustainable food production that meets both food security and climate and environmental objectives. The Common Agricultural Policy 2023–2027 provides greater access to financial support for moderate-scale farms and farms undertaking ecosystem restoration activities. This may affect the strength and direction of the relationship between farm scale and productivity, including technical efficiency.

This study describes a case of poisoning by pyrrolizidine alkaloids in a horse. To the best of the author’s knowledge, this is the first confirmed case of Senecio ovatus poisoning. A six-year-old 450-kg Irish cob mare was presented to the Equine Clinic of the University of Veterinary Sciences Brno (Czechia) with symptoms of hepatic encephalopathy, which progressively worsened with time despite intensive therapy and led to euthanasia. A complex diagnostic and therapeutic approach including the post-mortem patoanatomical and histopathological examination is described here. Regarding the histopathology of the liver, there was necrosis with haemorrhage, fatty changes and inflammation. A later inspection of the grazing area revealed the presence of Senecio ovatus (wood ragwort). A sensitive chromatographic method was used to determine the pyrrolizidine alkaloids and their metabolites in the plasma and the liver. In both of the samples, metabolites of pyrrolizidine alkaloids were detected. Although pyrrolizidine alkaloid poisoning was proven, the histopathological findings typical for this disease were absent. It is clear from our case that the histopathology in cases of poisoning by pyrrolizidine alkaloids may not always be conclusive.

Achieving the desired level of caramelisation in a date powder requires considerable effort. Consequently, an assessment was conducted on efficacy of thin-layer infrared dehydration for date paste. Various parameters were considered, including airflow velocities of 0.5, 1.0, and 1.5 m·s^–1, radiation intensities of 0.076, 0.1528, and 0.228 W·cm^–2, and date paste layer thicknesses of 3 mm and 5 mm. The study's findings indicated a positive correlation between drying rate and lowering airflow velocity and a negative correlation between drying time and decreasing airflow velocity, the thickness of date paste, and rising intensity of infrared. The effective moisture diffusivity (D_eff) was caculated by employing Fick's diffusion equation. For all situations under investigation, the mean values were within the range of 3.94 × 10^–11 m²·s^–1 to 6.01 × 10^–10 m²·s^–1. A relationship has been established between D_eff and moisture content. Seven distinct mathematical models were subjected to rigorous validation by applying non-linear regression analysis, aiming to accurately characterise the drying process of date paste. The modified two-term model provided the most accurate forecast of the drying process for date paste layers.

Actinidia arguta (Siebold et Zucc.) Planch. ex Miq. is functionally a cryptic dioecious plant and successful pollination is crucial for achieving high quality fruit. The extent and bases of morphological variability in female genotypes have been well studied, but here we focus on the males. Mature plants of seven male A. arguta genotypes were studied in 2016 and 2017 during which a suite of phenological and morphological features was measured on ten randomly chosen one-year-old canes on each plant. This analysis was complemented by two other, specialized measures potentially related to pollination efficiency, namely pollen quality, and quantity. The time of bud break was similar each year across all genotypes and the pollen quality was uniformly very high (viability - 95.0-99.9% and germination - 89.0-97.3%). However, the ten genotypes exhibited high variability in both the number of flowers per inflorescence and pollen quantity, indicating marked differences in their flowering potential and efficiency as pollinizers. The male kiwiberry indicators developed in this study - flowering potential and pollen efficiency, appear to be effective tools for the comparative evaluation of male A. arguta genotypes.

This study aimed to control land subsidence by improving the groundwater balance in the Varamin plain using the Groundwater Modelling System software and a multi-criteria decision model. For this purpose, aquifer level quantification and subsidence rate simulation were performed with the MODFLOW model and SUB package, respectively. The results showed a 6 m decrease in the aquifer level over a 5-year period and the subsidence rate in the central parts was 37 cm. Accordingly, the aquifer was evaluated by considering eight different restoration strategies based on reduced exploitation and artificial feeding. The results showed that the environmental criterion related to the subsidence adjustment index had the highest weight (0.27) and was introduced as the most important decision-making criterion. The evaluation of the results and priorities using the Complex Proportional Assessment (COPRAS) method showed that a 30% reduction in exploitation with artificial feeding is the best restoration strategy and can improve the subsidence rate and aquifer level by 36% and 76%, respectively, over a 5-year period (2024).

The Levels-of-Growing-Stock study for ponderosa pine was a collective effort among western Research Stations within the US Forest Service. The experiment was established to test sustainable productivity across a wide range of densities by periodically thinning the plots. Beyond the original purposes for wood production, contemporary applications of these long-term studies have been to determine stand density effects on (i) both overstory and understory responses to stand development of even-aged ponderosa pine, (ii) biomass accumulation and carbon sequestration, (iii) plant diversity and wildlife habitats, and (iv) forest resiliency to insects and pathogens, droughts, and wildfires. Furthermore, these installations have served as a showpiece for the public and natural laboratories for professional foresters and students. For the past half century, the study has helped guide land managers and stakeholders on public and private lands about the value of thinning in overstocked young stands of ponderosa pine across its range. We hope that it will continue to serve as a springboard for addressing future issues facing forest management.

Praziquantel (PZQ) is a drug effective against several parasitic diseases of fish caused by Platyhelminthes. The study assesses the efficacy of selected in-feed PZQ doses in eliminating metacercariae Diplostomum pseudospathaceum in grass carp. The fish were infected with D. pseudospathaceum in laboratory conditions and subsequently treated with PZQ at daily doses of 30 (PZQ1), 50 (PZQ2), and 100 (PZQ3) mg/kg bw for seven days. The treatment trial was followed by a 23-day observation period during which the fish were parasitologically examined on days 0, 2, 8 and 23. Smaller fish were infected with a significantly higher (P < 0.05) number of metacercariae than bigger fish among three weight groups as follows: up to 7 g > 7 to 12 g > 12 g. The in-feed PZQ at doses of 30, 50 and 100 mg/kg bw for seven days was 47%, 84% and 88% effective against metacercariae D. pseudospathaceum, respectively. A significant decrease (P < 0.01) in moving and an increase (P < 0.01) in the immotile metacercariae in the eye’s lens were recorded in all the trial groups on each sampling day. An increasing proportion of motile metacercariae in the post-treatment observation period was seen in PZQ2 and PZQ3, indicating a decreasing trend in the actual efficacy in an environment with no reinfection possibility.

Bovine ephemeral fever (BEF) is a vector-borne viral disease caused by the RNA virus which belongs to the genus Ephemerovirus and the family Rhabdoviridae. To evaluate the effect of the risk factors like the breed of cattle and buffaloes, age, sex, lactation, housing and region on the bovine ephemeral fever virus (BEFV) prevalence, ELISA and virus neutralisation (VN) tests (n = 600) were performed for the BEFV prevalence. The seroprevalence in cattle was 45.6% and 42% by ELISA and VN, respectively (P = 0.001). The breed-wise seropositive ratio was (55–64%) in cattle and (22.5–18.3%) in buffaloes by VN and ELISA. The sex-wise prevalence was (40–49.4%) in females and (35.8–46%) in males by VN and ELISA in cattle and a similar prevalence was reported in buffaloes. The age-wise prevalence in bovines by ELISA was 5.33, 22.66 and 17.66% in the age group < 1 year, 1–3 years and > 3 years, respectively. The disease prevalence was higher in the age group of 1–3 years. The prevalence was higher during the 3^rd lactation in bovines. The region-wise prevalence was higher in the 07 districts while lower (18–21%) in Rawalpindi District by VN and ELISA, respectively (P = 0.001). Commercial dairy farms of cattle showed a higher disease prevalence (52% and 44%) than non-commercial farms (38% and 36%) by ELISA and VN, respectively (P = 0.227). Exotic cows showed higher disease prevalence (76.67% and 70%) by ELISA and VN. The mortality in bovines was 5% (7.7% and 2.3%) in the cattle and buffaloes. The case fatality of BEFV in bovines was 12.25%. There was a significant effect of the risk factors like the breed, age, sex, lactation, housing and region on the BEFV prevalence. This is the first comprehensive study of BEFV in Pakistan.

Changes in the gene pool and homogeneity of red clover cultivars occur over time. Therefore, it is necessary to constantly renew the breeding material and foundation seed. Moreover, the market also prescribes a new demand. Based on the Natura 2000 habitats profile, we collected seeds from 39 locations across Lithuania in 2016. The phenotypic traits that affect the seed yield of red clover were analysed in 2018. The homogeneity of the populations with reference to the seed yield and 1 000 seed weight (TSW) were determined as well. Also, the possibility of obtaining two seed yields per season under drought conditions from wild genotypes of red clover was analysed. We found that the final seed yield mostly depends on the seed number per flower head (SN/FH), which strongly correlated with 1^st component of PCA during first (r = 0.91) and second (0.92) harvest. Meanwhile, the cluster analysis showed that the typical wild red clover has a lower seed weight than the cultivars and could be clustered on the basis of seed homogeneity. Finally, based on the seed phenotype and harvest components, there were five prospective accessions (2177, 2871, 2876, 2898 and 2899) for a new cultivar prototype.

The genus Alternaria has a global distribution and consists of a diverse group of pathogens. Plant-pathogenic Alternaria spp. can reduce the crop yield and pose serious threats to agricultural production. The pathogen A. mali is recognised as the key the pathogenic mechanism in the early defoliation of apples, which produces the host specific toxin (HST) that was named as an apple specific toxin (a specialised toxin of A. alternata pv. mali, AM toxin). The phenomenon of horizontal transfer of the AM toxin gene from different strains of A. alternata was found, and the relationship between the AM toxin and pathogenicity was confirmed. The representative strain A. tuberculata with the AM toxin gene was co-cultured with sixteen Alternaria strains without the AM toxin gene. As a result, four strains from different Alternaria species obtained the AM toxin gene, which indicated that the AM toxin gene can transfer among different Alternaria species. The AM toxin gene is easy to be lost after subculture, and high temperature and low nutrition can promote this loss. The symptoms of the Alternaria pathogen with or without the AM toxin gene are obviously different on the host. When infected by a pathogen with the AM toxin gene, green spots formed on the apple leaves, and rotten disease spots appeared in the fruit carpels. Contrary to this, when infected by a pathogen without this gene, only small epidermal spots without chlorosis formed on the apple leaves, and mildew-heart spots appeared in the fruit carpels.

In Saudi Arabia, tree mortality has abnormally increased over the past ten years, exceeding regulatory standards without clear interpretation. The present study aimed to investigate three attacked tree populations in different biogeographic regions of Saudi Arabia. For this purpose, climatic data from the last thirty years, forest densities, soil cover, pest search, and insect damage to trees have been reported. The results of this study show a distinct climate change manifested by an increase of 2 °C in temperature accompanied by a decrease in precipitation and the extension of drought periods during the last 30 years. Analysis of satellite images and the computation by quadrat methods revealed a decline in forest density and soil tree cover caused by dieback associated with an increase in the rate of tree pest attacks. Furthermore, tree investigations show attacks of the xylophagous beetles Steraspis speciosa and Paropta paradoxus on Acacia population in the north and centre of the country. In addition, many strains of termites are attacking the Dobera glabra population in the south of the Kingdom. This study shows that tree dieback, as a response to long periods of drought, when associated with pest attacks, provokes a dramatic decline in forest tree heritage. Climate change is the principal precursor to forest decline and pest invasion in Saudi Arabia.