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A study was conducted (I) to determine rumen degradability (in sacco) of dry matter (DM) and neutral detergent fibre (NDF) of the most important grass species grown in the Czech Republic, (II) to compare grass species (n = 5) according to calculated degradation parameters, and (III) to establish prediction equations for degradation parameters from chemical composition. Forty samples of the most important grass species (Dactylis glomerata, Phleum pratense, Lolium perenne, Festuca arundinacea, Felina hybrid) were harvested in 2004 and 2005 and analyzed for chemical composition, and DM and NDF in sacco degradability. Results from the in sacco method were used to calculate DM and NDF degradation parameters. Linear and stepwise multiple regression analyses were used to develop prediction equations for DM and NDF degradation parameters from nutrient concentrations. The highest DM and NDF degradation parameters were found for Lolium perenne. DM and NDF rumen degradation parameters were successfully predicted from chemical composition, with a single predictor adequate for most parameters. For all parameters R²-values increased with addition of more predictors into regression equations. Effective degradability (ED) of DM calculated at a rumen outflow rate (k) of 0.05 h^-1 was the best predicted by NDF (R² = 0.878), and ED of NDF at k = 0.02 h^-1 by crude protein (CP) (R² = 0.653).

The process of leukocyte cytolysis and the manifestations of apoptosis and secondary necrosis of neutrophil granulocytes (hereafter only "neutrophils") were studied on four virgin heifers after the induction of leukocyte influx into the mammary gland and after their lavage in in vitro conditions. Phosphate buffered saline, muramyl dipeptide and a lipopolysaccharide were used for influx induction. Cytolysis and apoptosis were induced with heat stress, ultraviolet irradiation and spontaneous aging for 24 hours. The cytolysis was detected indirectly by determining the lactate dehydrogenase activity in the cultivation medium after the enzyme was released through cell lysis. The neutrophil apoptosis was detected using flow cytometry and two staining methods (i) simultaneous staining with Annexin V labelled FITC and propidium iodide and (ii) with SYTO 13. It was found that leukocytes of the mammary gland of virgin heifers undergo spontaneous aging during in vitro incubation. The fraction of lysed leukocytes rose in the course of the in vitro incubation and reached 21% up to 34% after 4 hours and 73% up to 79% after 24 hours, depending on the inductor of influx used. From among them, phosphate buffered saline resulted in the lowest incidence of cytolysis, the lipopolysaccharide in the highest incidence. The differences in the effect of influx inductors on leukocyte cytolysis became manifest during the first 4 hours of incubation in particular; the differences between inductors became insignificant after 24 hours. Heat stress, unlike ultraviolet irradiation, resulted in a significant increase in the fraction of lysed leukocytes. Ultraviolet radiation induced neutrophil apoptosis in a dominant way, while the effect of influx inducers and/or of the staining method used for flow cytometry had no effect. Heat stress also induced neutrophil apoptosis but to a lower extent than ultraviolet irradiation. Spontaneous leukocyte aging during the in vitro incubation resulted in an increasing share of apoptotic neutrophils depending on the duration of incubation. An increase in the share of necrotic neutrophils was only significant after influx induction with the lipopolysaccharide, but not after induction with buffered saline. Highly significant correlation between the percentage representation of apoptotic neutrophils and the percentage proportion of lysed leukocytes was shown, both after influx induction with phosphate buffered saline, and with the lipopolysaccharide and after both staining techniques (r = 0.767; 0.932; 0.966; 0.922). Statistically significant correlation was demonstrated between the proportion of necrotic neutrophils and the share of lysed leukocytes only after influx induction with the lipopolysaccharide (r = 0.579; 0.765). After the influx induction with phosphate buffered saline and staining with Annexin V and propidium iodide, statistically significant negative correlation between the percentage share of necrotic neutrophils and the percentage of lysed leukocytes (r = -0.653) was demonstrated. Thus it means that situations can occur when the more leukocytes succumb to cytolysis, the smaller the share of necrotic neutrophils that can be detected with flow cytometry. One can state that the in vit

The first purpose of this experiment was to investigate the effect of ad libitum feeding of peat as a supplement to piglets from the age of five days up 23 days of age on their growth performance and health status. The second purpose was to assess the risk of the occurrence of conditionally pathogenic mycobacteria (CPM) in peat treated with ionizing radiation (Group PI) or per acetic acid (Group PP) and fed as a supplement to piglets. In respective experimental periods (at the age of 4, 23, 41 and 67 days), no significant differences in the average body weight between control group (C) and experimental Groups PI and PP were detected. Levels of selected biochemical (total protein, albumin, glucose, cholesterol, Ca, P, Fe and I) and haematological (erythrocytes, leukocytes and immunoglobulin - Ig) parameters of the health status of the piglets from all three Groups C, PI and PP were comparable at the age of 41 and 67 days. Mycobacteria were detected by culture in one diet sample (Mycobacterium intracellulare), in all 10 peat samples (7 M. a. hominissuis isolates, 2 M. intracellulare isolates and 1 M. xenopi isolate) and in 4 samples of biofilm from the drinking water pipeline system in the stables (M. xenopi, M. a. hominissuis, M. gordonae and Mycobacterium sp., one isolate in each). In 15 slaughtered pigs (at 67 days of age), no gross lesions that would give evidence of tuberculosis were found either in lymph nodes or parenchymatous organs. In Group C, mycobacteria were detected in tissues from two piglets (Mycobacterium sp. and M. a. hominissuis), Group PI in four piglets (M. a. hominissuis) and in Group PP in all five piglets (Mycobacterium sp., M. a. hominissuis, M. terrae and M. intracellulare). High positivity for CPM in both types of treated peat caused disseminated infection of the digestive tract of piglets from Groups PI and PP. Based on these results, feeding peat treated with ionisation or per acetic acid may be viewed as risky.

This case study describes the pregnancy of a German Shepherd bitch with a singleton (one puppy) litter as a result of early embryo resorption. Resorption was confirmed by ultrasonography and the pregnancy was regularly monitored by USG and measurements of progesterone levels until parturition. These levels stayed within the physiological range. One healthy puppy was delivered within the timeframe of a physiological pregnancy although the inner chorionic cavity diameter (ICCD) protocol used for predicting the time of parturition showed divergence from the real time of whelping.

Mycobacterium pinnipedii causes tuberculosis in free-living and captive pinniped species throughout the world. We report on the isolation of this M. tuberculosis complex (MTC) member from an imported male Southern sea lion (Otaria flavescens) in a zoo in the Czech Republic. Nodular granulomatous lesions were found in the lungs, pleura and mesenteric lymph nodes of this animal and M. pinnipedii was isolated from lung, mesenteric and submandibular lymph nodes. Identification of the isolates was confirmed using two independent molecular methods. Direct IS6110 PCR amplification confirmed the presence of an MTC member in these samples. Faecal and oral swabs from three living female sea lions were examined using direct IS6110 PCR and were all found to be negative. Twelve environmental samples were examined using direct microscopy after Ziehl-Neelsen staining and culture methods along with direct IS6110 PCR examination, all yielding negative results. Seven people that came into close contact with the infected animal were examined using a skin tuberculin test and chest x-ray, revealing no evidence of infection by a MTC member.

The integration of Central and Eastern Europe into both the European and the world economy was in the last decade associated with a relatively high growth of gross domestic product and with a gradual increase in the economic level. The processes of convergence at the national level, however, were accompanied by an unbalanced spatial development. The paper analyzes, on the basis of the empirical statistical data, the impacts of the first years of the membership in the European Union on the development of regional differentiation and on development disparities between rural and urban regions. The evaluation of regional development is performed in regional units at the levels NUTS 2 and NUTS 3. Rural regions are monitored at the level of regional units NUTS 3. The examination based on the NUTS 2 units would not allow identifying of such a large number of rural areas.

This paper presents detailed data on the biomass and element pools of six sample trees in the catchments of Plešné andČertovoLakes. Diameters and heights of the sample trees ranged from 28.0 to 63.7 cm and from 14.1 to 38.7 m. The age of the sample trees ranged from 84 to 177 years. Total biomass of the sample trees was in the range of 239.4 kg to 2,932.3 kg. Variation of total biomass between the sample trees was a consequence of the tree biometric data (height and dbh) and age differences. The proportion of stem wood and bark ranged from 63.5 to 69.5%, and from 4.6 to 7.2%, respectively. The proportion of foliage and fine branches ranged from 4.3 to 8.4%, and from 0.7 to 1.9%, respectively. The proportion of branch wood and bark ranged from 2.2 to 6.5%, and from 0.8 to 2.2%, respectively. Mean concentrations of C in different tree components were quite similar. Except C and compared to the other elements, N had the highest mean concentrations in tree components in all cases. Concentrations of P, Ca, Mg, and K showed similar patterns. Generally the highest concentrations of these elements were found in foliage, fine branches, fine roots and bark of stem and branches. Fe, Na, Al and Mn showed the lowest mean concentrations in tree components for all the analyzed elements. The total element pools per tree were highly variable because of the differences in total biomass between the individual trees. Generally, stem wood and bark, foliage, and roots contained the highest proportion of the elements. But there were differences between individual elements. Concerning the important nutrients, while the highest proportion of Ca and Mg was contained in stem wood and bark, the highest proportion of P was contained in foliage. The foliage contained a relatively high proportion of P and K, but a relatively low proportion of Ca and Mg.

A wide array of antioxidative and anti-inflammatory substances derived from edible plants have been reported to possess chemopreventive and chemoprotective activities. Among the most extensively investigated and well-defined dietary chemopreventives is curcumin. Using the Ames test and in vivo micronucleus test, chemiluminescence test, blastic transformation test, and comet assay, we examined the antimutagenic effects of the chemically identified chemoprotective substance curcumin (diferuloylmethane) in the pure form on mutagenicity induced by three reference mutagens: aflatoxin B₁ (AFB₁), 2-amino-3-metylimidazo[4,5-f]quinoline (IQ), and N-nitroso-N-metylurea (MNU), and the effect of curcumin on the immunosuppression caused by these mutagens. Curcumin in the pure form showed a clear antimutagenic and immunomodulatory activities on mutagenicity and immunosuppression induced by reference mutagens.

In Brazil savanna type of soils presents problems with phosphorus content. The selection and identification of maize genotypes to such environments is a high priority of Brazilian research. The purpose of this paper was to evaluate, in soils with different P concentrations, the dry mass attributes and characteristics of root morphology in eight maize lines with different genetic background and origins of the Breeding Program of the National Research Center for Maize and Sorghum. The experiment was carried out in plots prepared with two levels of phosphorus: high phosphorus (HP) and low phosphorus (LP). The experimental design was randomized blocks with three replications. The evaluation of the characteristics of the shoots and the root system morphology was performed 21 days after sowing. The WinRhizo program of images analysis was used for the root morphology. There were no differences between the phosphorus levels for the dry mass attributes. However, when we compared P levels, root morphology of L13.1.2 strain performed the highest surface area (SA) and total root length (RL), length of thin (TRL) and very thin (VTRL) roots in low P concentration. The root systems digital images analysis techniques allowed efficient discrimination of maize genotypes in environments with low P levels.

Investigations were carried out at the experimental field of the Faculty of Agriculture in Zagreb in the growing seasons 2008/2009 and 2009/2010 with the aim to determine the influence of seeding rate and fungicide treatment upon the yield and yield components of spelt (Triticum spelta L.). The trial included two spelt cultivars: Nirvana and Ostro, three seeding rates: 200, 300, and 400 germinated seeds/m2, and a fungicide treatment (tebuconazole). Cultivar Nirvana produced a significantly higher grain yield in both trial years, higher number of spikelets per spike, higher grain number and mass per spike. Cultivar Ostro had a significantly higher grain crude proteins content, higher 1000 grain weight, and also a higher number of sterile spikelets per spike. No significant effect of seeding rate on grain yield was determined while the fungicide treatment had a significant effect on grain yield only in the warmer year 2008/2009.

In conifers and other plants with long reproductive cycles, transformed embryogenic tissues can serve as a convenient source of plant material for the testing of insecticidal or fungicidal transgene efficiency. In this report, transgenic embryogenic tissue was obtained after the transformation of somatic embryos of Norway spruce (Picea abies (L.) Karst.) by Agrobacterium tumefaciens with the gus-intron chimeric gene. The stable integration of transgenes was confirmed by PCR and Southern hybridization. The transformation was successful only in a suitable embryogenic cell line sensitive to Agrobacterium. Out of the nine embryogenic lines tested only one gave transgenic callus.

The presence of genes for resistance to scab (Venturia inaequalis) and powdery mildew (Podosphaera leucotricha) was studied using molecular markers in a sample of 279 apple cultivars from the Czech collection of apple genetic resources. The sample comprised 37 cultivars supposed to have the Vf gene for scab resistance, 97 reference world cultivars and 145 old and local cultivars. Six PCR molecular markers for the scab resistance genes Vf, Vm, Vbj, Vr and Vh and three PCR molecular markers for the powdery mildew resistance genes Pl-w, Pl-1 and Pl-d were used. The marker for the major scab resistance gene Vf was detected in all cultivars supposed to have Vf, except in Romus 1, and in the three small-fruited cultivars Malus Evereste, Golden Gem and Hilleri. The markers of the Vr and Vh scab resistance genes were detected in 22 cultivars in combination with the marker for Vf, in 56 reference world cultivars and in 82 old and local apple cultivars. PCR molecular markers for one or two of the powdery mildew resistance genes were detected in the small-fruited cultivars Malus Evereste, Golden Gem, prof. Sprengeri and Hilleri; and in the larger fruited cultivars Hagloe Crab, Borovinka and Tita Zetei. We did not find markers for the scab resistance genes Vm and Vbj in any of the studied cultivars. They are absent also in the remaining part of the Czech collection of apple genetic resources. PCR molecular markers are useful tools for the identification of resistance genes within apple germplasm collections and can be used to increase the number of sources for disease resistance in breeding programmes.

Chickpea chlorotic dwarf virus (CpCDV, genus Mastervirus, family Geminiviridae) is the most common viral disease of chickpea in Pakistan. Two aphid [Aphis craccivora Koch, Myzus persicae (Sulzer)], two leafhopper [Empoasca devastans Distant, Orosius albicinctus (Distant)] species and an unidentified brown leafhopper were collected in a chickpea field by hand and sweep nets for transmission studies of CpCDV. Transmission results showed that only the leafhopper O. albicinctus successfully transmitted the CpCDV from diseased to healthy chickpea plants. The presence of CpCDV in inoculated plants and the vector O. albicinctus were confirmed by DAS-ELISA test using specific polyclonal antibodies.

Clinical examination and emergency care in newborn puppies is difficult, due to their different physiological characteristics and needs from those of adult dogs. This paper reviews the physiological events during parturition and the influence of parturition on puppy health.

The study has been elaborated on the basis of a continuous analysis of economic results of a selected sample of agricultural enterprises operating in different climatic and productional conditions. Long-term tendencies and their influencing factors are defined on the basis of this analysis. The goal of this study is to evaluate the influence of external conditions on the economy of agricultural enterprises and on their economic results and to analyze the influence of the accession of the Czech Republic to the EU on the change of economic conditions of agricultural enterprises. On the basis of this study, the authors measures which would intensify the influence of agricultural enterprising on the rural development and which would improve the quality of life of rural inhabitants.

A disadvantageous tendency of resigning from milk production by small and medium sized farms has been observed in the recent years in the provinces of Southern Poland. It results from the low profitability of milk production on these farms and therefore the difficulties in reaching the income parity. It leads to a decline in the bovine population not compensated by an increase in the yield per animal and it poses a serious hazard to protecting the natural resources which is the function dairy farms perform in this region. The problem is serious since in the analyzed region, milk is acquired from 75% of farms keeping herds of up to 10 cows. Moreover, grasslands constitute a notably high share in the arable land structure, and for maintaining them cow keeping is a crucial condition. Hitherto functioning system of EU payments proved insufficient; therefore the Polish government intends to provide an additional financial support for dairy farms. The authors intended to reason that despite the hardly important participation of the analyzed regions in total milk production in Poland, an additional support for small and medium sized dairy farms from the state budget is socially necessary, in the first place because of the additional functions fulfilled by these farms.

Organic manure is considered as a beneficial fertilizer on soil quality and an excellent alternative resource of chemical fertilizer (CF). However, organic manure from intensive farms may have a negative impact on soil quality because of containing some harmful components, such as heavy metal and antibiotics. The aim of this study was to determine the influence of poultry litter (PL) and livestock manure (LM) from intensive farming on soil physical and biological indicators of soil quality. Results showed that PL and LM amendment increased soil macropore and mesopore volumes and decreased soil micropore volumes. Tensile strength in PL and LM treatment were lower than those in CF, while soil aggregate wet stability index were greater than those in CF. Compared with CF treatment, the microbial biomass C and N contents (+89%, +74%), soil basal respiration rate (+49%) and soil microbial quotient (+45%) in PL and LM treatment were significantly greater. Significant linear correlations were found between soil organic carbon and most soil physical and biological properties (P < 0.01). The results suggested that modern intensive farm manures can be alternate chemical fertilizers as a main fertilizer to improve soil physical and biological indicators in a rice-wheat system.

The present paper is aimed at the use of satellite navigation of field machinery during seeding, this operation belonging to the most important field practises. Our attention was focused on the determination of the accuracy of the satellite navigation system based on using the correction signal real-time kinematic and its correct application for planting a wide-row crop (sunflower) and seeding a narrow-row crop (spring barley). The aim of the field experiment was also to specify the level of the necessary accuracy of satellite navigation systems during planting and seeding. The length of seeding/planting equipment was confronted with the accuracy of navigation of individual passes, especially when turning on the headlands. In the conclusion, the importance is highlighted of the automated tractor headland control during satellite navigation of combined field machines in the crop production.

The problem of international debts is, by its character, one of the most complex problems which the world economy is now facing. It complicates both the global balance of payments and the financial - credit system stability, with pronounced negative impacts on the currency stabilisation. It hinders the development of international economic co-operation and its higher forms - international economic integration. It is one of the reasons that the symmetrical forms of interdependence are pushed off and displaced by the asymmetrical ones. The global debts problem deteriorates, namely during the last time in interaction with the negative manifestations of the global problems (for example, at present very strongly with the environmental problems), the complex international co-operation climate.

We examine the effects of selected fungicides on the Fusarium growth and toxins production especially of DON and T-2 toxins. Appropriate nutritive media were prepared containing different concentrations of the fungicides tested (Horizon 250 EW and Falcon 460 EC), inoculated with Fusarium moulds, and incubated under various conditions. The extraction of Fusarium toxins and their determination were carried out after the incubation using Agra Quant^®Deoxynivalenol Test kit and Agra Quant^® T-2 toxin Test kit. The results indicated a considerable variability of the individual strains in the formation of toxins. The strains of Fusarium graminearum CCM F-683 and Fusarium spp. (isolated from wheat) produced large quantities of both DON and T-2 toxins. Fusarium poae CCM F-584 and Fusarium sporotrichioides CCM F-352 always produced larger quantities of only T-2 toxin in the given nutrient media. The experiment showed that the use of lower concentrations of fungicides resulted in an increased production of the monitored mycotoxins. The presence of Tri5 gene in the tested Fusarium strains, coding trichodiene synthase, was confirmed by polymerase chain reaction. The enzyme - trichodiene synthase - catalyses the isomerisation and cyclisation of farnesyl phosphate to trichodiene, the first step in the biosynthetic pathway of trichothecenes.

Bread-making quality of wheat flour is influenced by wheat storage proteins. The percentage of sodium dodecyl sulphate (SDS)-unextracted polymeric proteins in total polymeric proteins (% UPP), as one important factors determining bread-making quality, is largely influenced by the individual and interactive effect of genetical background and environmental conditions. Four spring wheat cultivars were grown in green house. Two of the cultivars have high molecular weight glutenin subunits 5+10 while the other two have 2+12. Four different nitrogen regimes and two different temperature levels (distinguished as low and high temperature) were used. The results highlighted the importance of interactive influence of cultivars, nitrogen regimes and temperature levels for creating differences in the amount of % UPP during grain maturation period (GMP) and at maturity. The shift to end up with either high or low % UPP at maturity happened early during GMP (around 12 days after anthesis). Influences of cultivars on % UPP was found early during the GMP while influences of temperature and nitrogen regimes were seen much later during the GMP. The results will help in understanding the relationship between quality parameters of wheat flour and how these are influenced by different environmental and genetic factors.

Virulences to powdery mildew resistances in barley cultivars mostly carrying unknown resistances were determined in 2009 and 2010. Random spore samples of the airborne pathogen populations originating from winter and spring barley fields were obtained by means of a mobile version of a jet spore sampler by travelling across theCzech Republic. In total 301 isolates were studied, 55 differentials carrying mostly unknown resistances were used and 80 pathotypes were found, of which 26 representing 73.1% of isolates were detected in both years. Virulence frequencies showed a wide range from 0% to 100%. Complexity of the 2010 population slightly increased, mostly due to increasing frequencies of virulence to new resistances, whereas the complexity of virulences to resistances in most other differentials decreased. Pathotype 00027 was the most abundant (10.0%). Diversity of the 2010 population considerably increased due to changes in virulence frequencies.

We have estimated soil organic carbon and crop yield changes under distinct climate change scenarios for the Kočín farm in Slovakia. Two regional climate change scenarios, i.e. the A2 and B2 SRES emission scenarios, and a reference climate scenario have been included into the bio-physical process model EPIC to simulate the effects on the topsoil organic carbon stocks and crop yields for the period of 2010-2050. In addition, we have used the data from several fields of the Kočín farm including the soil data, crop rotational and management data as well as topographical data. The topsoil organic carbon stocks show a decreasing trend for the period of 2010-2050. Among all crop rotation systems and soil profiles, the losses over the period are 9.0%, 9.5%, and 10.7% for the reference, A2, and B2 climate scenarios, respectively. Increasing temperatures accelerate the decomposition of the soil organic carbon particularly when soils are intensively managed. The soil organic carbon changes are crop-rotation specific, which is partly due to the climate scenarios that affect the crop biomass production differently. This is shown by comparison of the crop yields. We conclude that EPIC is capable to reliably simulate effects of climate change on soil organic carbon and crop yields.

The article presents the results from the monitoring analyses of the buying/selling prices of agricultural land in accordance with the deposited contracts in the Real Estate Cadastre in years 2001-2008. Agricultural land sales, land areas and market prices are observed and evaluated under the size structure of the sold estates and their anticipated further utilization in the counties Dunajská Streda, Topoľčany, Rimavská Sobota, Liptovský Mikuláš, Michalovce and Svidník and for all observed counties as a whole. By data analyzing and evaluating of the agricultural land purchases/sales, there was taken into consideration the fact that the average price of the sold estates is to a large extent influenced by their size, location and the purpose of further utilisation. The estates of small area characterized as agricultural land are selling for the highest prices, but they already figure in the development studies and the land-use plans of the villages or they are regarded as building estates in the future. Therefore, the sold estates were divided into two categories in accordance with the expected further land utilization. As the estates for further agricultural utilization, there were considered the estates with the area above 1 hectare of agricultural land. In the case of smaller estates, it is assumed that they will be of building, recreational, sporting or other utilization after the landowner change.

This article examines the factor content of agricultural trade in the Central and East European (CEE) transition countries. It relates the factor content of agricultural trade to the cross-country differences in technology stemming from different farm organisations between the CEE countries agricultural sectors and to the differences in relative factor endowments. The relative factor endowments alone do not satisfactorily explain agricultural trade flows in the CEE countries. We find that transaction costs and market imperfections that affect the organisation of production also distort farm specialisation and hence V the actor content of agricultural trade.

In this experiment we proved an effect of dolomite limestone on chemical immobilization in soil contaminated by trace metals, namely Cd, Pb, and Zn. Primary, we set up lysimeter pot experiment to measure soil leaching without vegetation. Willow clone (S. × smithiana Willd) was cultivated in the second lysimeter pot as a new approach to monitor Pb, Cd and Zn leaching, which was affected by soil liming (used in 1% rate). At the time of both harvests, aboveground biomass increased significantly at the amended variant. After the second harvest aboveground biomass production increased by 80% in comparison with the first one, Cd and Zn concentration in biomass decreased 2-fold and 3-fold, respectively. Dolomite limestone as a process of liming: (i) restricted metals leaching from the soil substrate; (ii) reduced metal uptake by willow; and (iii) increased biomass production of willow. Liming also alleviated the plant stress imposed by risk elements resulting in better plant growth and lower levels of stress markers (total nitrogen content and the main amino acid metabolism parameters in the willow leaves) yet through different mechanisms.

Hop purchasers impose still higher requirements on the quality of the final product, which is why hop granules have to be divested of all possible impurities. One of the places that are most at risk from the given point of view is the making of hop strings and the way they are attached to the hop-field supporting structure. Commonly used hop string attachments very often remain on the supporting structure after the harvest, spontaneously loosen in the following years, penetrate into the post-harvest processing stage of the technological procedure and negatively influence the output quality of the hops. The article summarizes the results of field experiments done during the pull-down of hop vines using a common hop string, but with various attachments with the aim of looking for the most suitable treatment which would exclude the risk of impurities penetrating from this stage of the technological procedure into the final product. The measurement carried out shows that so far the most advantageous treatment is hop string with simple attachment formed by twine 12 500. Breakage of hop strings occurred in 93% in twine, while with the rest of the vines only in wire.

Our study aimed to determine morphometric data for wild boar (Sus scrofa) in various areas of the Czech Republic and the potential influence of environment on its body measurements. Three localities with varying agricultural systems and overall landscape structure were selected. Hunted boars were measured for height at the withers, body length, ear length, metatarsal length and weight (depending on the circumstances, either dressed with head, without head, or undressed). We also determined the age of the hunted boars according to teeth development. During 2003-2007, a total 654 boars were examined in various age categories. Body development was similar in all areas and without statistically significant differences until the age of 6-7 months. From 8 months, statistically significant differences in body proportions occur across all localities. It is just at that time that carrying capacities change in the selected localities. The results show that morphometric differences among boars of the same age are influenced by external environmental conditions in which the boars live.

Ornamental nurseries, garden centres, public gardens and urban greenery in the Czech Republic were surveyed in 2006-2009 for the presence of Phytophthora spp. and the diseases they cause on ericaceous plants. Diseased plants such as Rhododendron spp., Pieris floribunda, Vaccinium sp., and Azalea sp. showed various symptoms including leaf spot, shoot blight, twig lesions or stem, root and collar rot. Nearly 140 Phytophthora isolates were collected from symptomatic plants in different areas of the country. Of the Phytophthora spp. on ericaceous plants or in their surroundings, P. plurivora appeared to be the most common species. Herein, we focus on the most frequently occurring species, P. plurivora, and describe its morpho-physiological and pathogenicity features and confirm its identity based on ITS sequences of rDNA. In addition, we give a list of other Phytophthora spp. including P. cactorum, P. cambivora, P. cinnamomi, P. citrophthora, P. megasperma, P. multivora, P. ramorum, and P. gonapodyides that we identified on the basis of their cultural and morphological characteristics and DNA sequences. We also discuss their importance in cultivated and natural ecosystems.