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This study aims to explore the optimum nitrogen (N) application method by analyzing effects of variable N application stages and ratios on the N absorption and translocation of high-yield summer maize (DH661). The study included field experiments and ¹⁵N isotopic dilutions for pot experiments. Results showed that the yield was not increased in a one-off N application at the jointing stage. The uptake of fertilizer-derived N in the grain increased with the increasing of N applied times. Compared to a single or double application, total N uptake (N_up) and biomass increased significantly by supplying N at the six-leaf stage (V6), ten-leaf stage (V10) and 10 days after anthesis in ratios of 3:5:2 and 2:4:4. The fertilizer-derived recovery rates were 67.5% and 78.1%, respectively. The uptake and utilization of fertilizer-derived N was enhanced by increasing the recovery rate of N supplied after anthesis, and reducing the absorption of soil-derived N. Therefore, the 2:4:4 application ratios was the optimal N application method.

Relatively little is known about potassium leaching losses following harvest of silage maize. While direct negative impacts on the environment are unlikely, losses of K with leaching need to be known for accurate balancing, especially on coarse textured soils, where K can be a critical element. In a four-year field experiment the effects of fertilizer forms (inorganic, cattle slurry and pig slurry) and four levels of N input (0, 80, 160, 240 kg N/ha) with corresponding amounts of K on the nutrient balances and leaching of K from silage maize grown on a sandy soil were investigated using suction cups. After four years, surplus of K from cattle slurry led to higher lactate-soluble K in the topsoil. Potassium leaching differed between years with different amounts of rainfall during winter. Annual leaching losses of K increased with N and K input and amounted to 38 kg K/ha, while fertilizer form had no significant effect. Losses of K increased with increasing N leaching (R² = 0.69). We conclude that in maize production on coarse textured soils and under conditions of high N leaching (86-152 kg N/ha), K leaching can be large (6-84 kg K/ha) and constitutes a relevant part of K balances (-84 to +127 kg K/ha).

In this study, productivity rate and operation cost of farm tractor were evaluated in a mountainous natural forest. Data for the study came from a detailed time study in the Research and Educational Forest of Tarbiat Modares University (REFTMU) and was used to develop a multiple linear regression model to predict the skidding cycle time. The results showed that effective independent variables of skidding time were skidding distance and slope of skid trail (P = 0.01). Average productivity rate ranged from 2.43 to 2.60 m².h^-1. Total time and effective time, respectively. Total cost of the system was 10.24 USD.h^-1 whereas 4.58% of the cost of skidding resulted from personal delay. The cost and productivity resulting from this study emphasized the importance for foresters to consider a farm tractor when designing skidding operations in young stands or prescribing a combination of two machines in mature stands.

The pine stand in the Havraní Skála locality in the Bohemian Switzerland National Park was affected by an extensive mixed-severity wildfire in summer 2006. The fire severity, fire type and fire extent were assessed by various fire severity measures collected in the field (mean bole char height on stems on sample plots, content of oxidizable C and total N at a soil depth of 0-5 cm), as well as by classification and filtering of green canopy cover from time series of aerial photographs acquired before the fire (2005), soon after the fire (2006) and one year after the fire (2007). The specific image analyses made it possible to uncover the spatial and temporal pattern of the stand defoliation. The central part of the site was mainly affected by the crown fire and thus defoliated substantially right during the fire. On the contrary, the peripheral part of the site was mostly affected by low-severity ground fire and therefore defoliated progressively one year later. All the fire severity measures used were well related.

The prevalence, distribution, and antibiotic susceptibility of Staphylococcus aureus was investigated in ready-to-eat (RTE) salads, the environment, and the personnel of a salad producing plant in Greece. Staphylococci were isolated from 27% of the samples. Apart from three isolates which were sensitive to all antibiotics, all the others exhibited resistance from two up to five antibiotics. None of the isolates was resistant to oxacillin. Random Amplified Polymorphic DNA (RAPD) analysis showed no genetic relation between the human and salad isolates while one RAPD clone of S. aureus predominated among the salad samples. The results suggest that an effective application of Good Manufacturing Practices (GMP) is needed along the food production chain to prevent the contamination of RTE foods.

In 2009-2011 virulence of the wheat leaf rust population was studied on Thatcher near-isogenic lines with Lr1, Lr2a, Lr2b, Lr2c, Lr3a, Lr9, Lr11, Lr13, Lr15, Lr17, Lr19, Lr21, Lr23, Lr24, Lr26 and Lr28. Samples of leaf rust were obtained from different parts of the Slovak Republic. A total of 122 wheat leaf rust isolates were analysed. Resistance gene Lr19 was effective to all tested isolates. Virulence to Lr9 was found, however only in one isolate. Gene Lr24 conditioned resistance to almost all rust collections. A lower frequency of virulence to Lr2a and Lr28 was also observed. Nineteen winter wheat cultivars grown in Slovakia were tested with 8 leaf rust isolates. Winter wheat cultivar Bona Dea was resistant to all isolates applied in the greenhouse test. Presence of Lr genes was estimated according to the reactions of the tested cultivars. Presence of Lr10, Lr26, Lr34 and Lr37 was studied by molecular markers.

The dynamic coefficient of friction of single potatoes was determined on mild steel, rubber and plastic, for tubers in each of four conditions: dry and dirty, wet and dirty, clean and wet and clean and dry. Steel had the lowest overall mean coefficient of friction. The highest value was recorded for plastic, but this material also had the largest coefficient of variation. Overall, rubber was considered to be the most suitable all-condition material for tubers in a range of conditions.

Chemical seed protectants are used to reduce the adverse effects of seedling fungal pathogens or insect attack on legume pastures and crops. Chickpea seeds are also frequently treated with Mesorhizobium sp. Cicer inoculant to promote effective symbiotic nitrogen fixation (SNF), which seems to be a cost effective measure. The population of viable Mesorhizobium sp. Cicer on seeds of chickpea declined with time of storage (4°C) in pesticide treated and untreated chickpea seeds in vitro. A significant reduction in chickpea rhizobia was observed in seed treatment with Captan followed by Endosulfan and Chlorpyrifos. In a field experiment during the winter season 2006-2008, no difference in the emergence count of chickpea plants was observed. Treatments inoculated with Mesorhizobium sp. Cicer alone or along with Captan, Chlorpyrifos or Endosulfan showed improved plant growth and symbiotic parameters (plant height, nodulation, leghaemoglobin content, and nitrogen content) in comparison with the uninoculated control treatment. Significantly higher grain yield (9.6%) was observed in the treatment inoculated with Mesorhizobium sp. Cicer alone as compared to the uninoculated control. A non-significant difference in grain yield among treatments where Mesorhizobium sp. Cicer along with a mixture of fungicide and insecticides was applied was observed in contrast to the Mesorhizobium sp. Cicer treatment. In conclusion, the recommended rates of fungicide and insecticides as seed treatment were not detrimental to chickpea-Mesorhizobium sp. Cicer symbiosis, hence they can be safely used to obtain higher productivity.

Relationships between sensory variables, species, feeding systems, and panelists were examined by Procrustes Analysis. Six meat groups considering species and feeding type combinations were evaluated by ten panelists for five sensory characteristics (colour, texture, odour, taste, and acceptability). These characteristics were assessed using a nine-point hedonic scale (9 = extremely enjoy, 1 = extremely dislike). Generalized Procrustes Analysis was performed and 61.11% of the total variability was explained by the first two dimensions that correspond to Factor 1 and Factor 2. The first one accounted for 33.99% and the second for 27.12%. Results showed that pasture conditions were reflected in all sensory characteristics of meat of Hair goat as well as Karakas sheep. This study concluded that meat of sheep and goats reared in pasture conditions was more preferable as concerns sensory characteristics than that of fattening animals or those reared in intensive conditions.

This paper reviews hypertrophic osteopathy and describes one case report. Hypertrophic osteopathy is a rare pathologic disease process and is observed secondary to a mass in the thorax. In response to the presence of a mass(es), nonoedematous soft tissue swellings and a diffuse periosteal new bone formation develop in all four limbs. The result is mild to severe lameness. A twelve-year-old sexually intact female Cocker spaniel had undergone radical mastectomy on both sides in another veterinary hospital about two years before presentation in our hospital with lameness of both hind limbs. Pain and soft tissue swelling on the distal parts of extremities were determined in clinical examinations. Radiographs revealed periosteal new bone formation on all the long bones of all four limbs, pelvis and sternum; additionally, intrathoracic masses were observed. Euthanasia was performed five months later. Macroscopic examinations of the lungs revealed diffuse and exuberant masses with grizzled whitish cross-sectional colour and with necrotic and haemorrhagic foci. The radius-ulna, tibia, metacarpal and metatarsal bones of both limbs were examined and collected after the necropsy examination. Bone specimens were thicker and the outer surfaces seemed to be rough. At the histopathologic examination of the lung tissue, ovoid or round shaped and hyperchromatic nucleated diffuse anaplastic mammary gland epithelial cells were observed. According to these findings, these masses were diagnosed as the metastasis of malignant mixed tumours.

Transmissible venereal tumours (TVT) are normally seen on the genitalia of both male and female dogs, and at times may be observed on extra-genital sites such as lips, oral mucosa, and peritoneum, or in organs such as the tonsils, eye, liver, spleen, kidney, lung, and musculature. The present communication deals with two rare cases of primary extragenital TVT involving the mammary glands of dogs and their pathology and immunohistochemistry. The study indicated that apart from routinely used markers such as vimentin, p53, PCNA, Ki-67 and c-myc, the oncogenes Rb and cyclin D1 proved to be novel markers of TVT in dogs. To the authors' knowledge, this is the first report of extra-genital mammary TVT in canines.

The parasite load of brown hares (Lepus europaeus) is of great interest to hunting ground managers and veterinarians. We compared the prevalence and intensity of parasitic infections in 362 hares from Austria and the Czech Republic with respect to age and body weight. Samples of the entire gastrointestinal tract, liver and lungs were collected during autumn hunting events in 2007. The parasite spectrum of hares included Protostrongylus pulmonalis, Graphidium strigosum, Trichostrongylus retortaeformis, Trichuris leporis, Eimeria spp. and tapeworms. The most prevalent gastrointestinal nematode was Trichostrongylus retortaeformis, while only individual specimens of tapeworms such as Andrya rhopalocephala, Mosgovoyia pectinata, Cittotaenia denticulata and Ctenotaenia ctenoides were found in subadult hares. A single hare was infected with Cysticercus pisiformis in Austria. Lungworms Protostrongylus pulmonalis and findings of pneumonia were significantly less prevalent in subadult than adult hares (P < 0.01) from both countries and were much less prevalent overall in the Czech Republic (P < 0.01). Graphidium strigosum, Trichostrongylus retortaeformis, Eimeria spp. and enteritis were more prevalent in subadult hares. The nematode Trichuris leporis, on the other hand, prevailed in adults. The body weight of adult hares was negatively correlated with the intensity of infection by Protostrongylus pulmonalis (r = -0.67) and Trichostrongylus retortaeformis (r = -0.73) and the parasite loads served as significant weight predictors in multiple regression equations. This study revealed that parasitic infections of the lungs and intestines influences the health and decreases the body weight of hares in Austrian and Czech hunting grounds.

The paper addresses the initiative Tradice Bílých Karpat. It is the example of innovative use of local resources through joining together organic farmers and environmentalists. Content analysis (research technique used in combination with case study) proved to be an efficient tool when evaluating the impacts of this initiative for the public. The results documenting the impacts of this initiative for the public are rather of regional nature. They address only some activities of the Tradice Bílých Karpat, mostly those concentrated around processing of local apples. The paper also outlines the possible reasons for the existing image of this initiative presented in mass media and presenting it for the public.

Agricultural land market is usually defined as the purchase of agricultural land. In the most of the European countries, the land rental transactions have received the dominant position in the land market. Therefore, the land rental transactions are included into the agricultural land market analysis. The decision processes of market subjects are often influenced by the law. On the one hand, there is the law regulating the value of agricultural land from the various points of view. On the other hand, there are some statutes stipulating the rental legal rules. The objective of this paper is to analyse the influence of the law on the behaviour of market subjects in the agricultural land market.

The paper is the 6^th report on the production potential and stability of mixed forest stands in uplands. A mixed beech/larch stand that was established by natural regeneration in 1934 to 1942 is assessed. The stand is situated at an altitude of 460 m above sea level. It has been left to its natural development since 1961. At that time, the stand was characterized as an individually mixed, diameter- and height-differentiated 25-year pole-stage stand. The proportion of larch and beech amounted to 40% and 17%, respectively. Hornbeam (25%), oak (11%) and to a lesser extent birch (5%) and spruce (3%) also occurred in the stand. In the course of 42 years, the proportion of larch in this stand without planned thinning measures decreased to 35%. On the other hand, the proportion of beech increased to 39%. During all 5-year inventories, the stand could be characterized as a stabilized one with high production potential. Its initial growing stock 63 m³/ha at an age of 25 years increased to 497 m³/ha at an age of 67 years in 2003. At present, current volume increment amounts to 9.8 to 12.5 m³/ha/year.

The effect of inoculation with Pseudomonas fluorescens and Bacillus subtilis on the yield of fresh and dry mass of English ryegrass (Lolium perenne L.) as well as on the number of rhizospheric microorganisms was studied. The microorganisms were introduced into the soil before sowing. The control plots were not inoculated. The number of microorganisms was determined after the third mowing. The yield was determined after the first, second and third mowing. In comparison with the control, after the first and second mowing, there was a statistically significant increase in the fresh and dry mass in both inoculated variants whereas after the third mowing, a statistically significant increase in the yield of fresh mass was recorded only in the variant with B. subtilis. The use of B. subtilis had a better effect on the total yield of the fresh and dry mass of English ryegrass. The number of the investigated groups of microorganisms, apart from actinomycetes, increased in the inoculated variants. Inoculation of P. fluorescens affected the increase of total number of bacteria and aminoheterotrophs whereas inoculation of B. subtilis affected the increase of the number of azotobacter and fungi.

As a part of International Cooperative Programme on Assessment and Monitoring of Air Pollution Effect on Forests (ICP Forests), defoliation of forest stands has been assessed in the Czech Republic since 1986. Defoliation is one of the principal parameters reflecting the health of forest stands that is assessed on monitoring plots within this programme. Monitoring plots are distributed evenly according to woodiness of the area in a regular schematic network all over the Czech Republic. Even though there has been a long-term reduction of air pollution in most European countries, forest ecosystems reflect these changes with some delay. In the Czech Republic the trend of total defoliation of coniferous and deciduous forest stands has been increasing very moderately in the last 10 years. This trend appears to be partially analogous with some neighbouring countries. Compared to the whole of Europe the trend of defoliation of broadleaves is very similar, but differences have occurred in conifers in the last 10 years. There are significant differences in defoliation levels among the particular countries; in this comparison the Czech Republic is classified as one of the worst. Differences in these levels are very evident especially among the neighbouring countries. To partly eliminate these differences, regular international calibration courses are organized.

The paper deals with the analysis of price changes of roundwood (spruce, fir and beech sawlog, fuelwood) and energy wood in Slovakia, Czech Republic and Austria during the period of 2008-+September 2011. At the beginning of the article, the authors identify the major factors influencing timber price trends such as legislation regarding technical standards for the quality grading of round wood, global economic recession, incidental felling and political influence. Consequently, detailed analysis of timber price changes in selected European counties is provided by means of line graphs. The results of the analysis revealed that demand for wood and wood products began to decrease in mid-2008. The most critical situation was in 2009, primarily in the softwood market, when prices bottomed out. However, recent trends indicate a gradual rise in timber prices. In the future, a slight increase of timber prices is expected, however other factors (such as economic development, lobbyist pressure, incidental felling and many others) may influence prices, which makes it difficult to predict their trend.

The effect of thickening agents - methyl cellulose, hydroxyethyl cellulose, sodium carboxymethyl cellulose, and xanthan gum - solutions on the sensory viscosity was investigated in the concentration range of 0-0.8%. The perceived viscosity was proportional to the logarithm of kinematic viscosity in the presence of citric and malic acids. The viscosity was inversely proportional to the acidity at the viscosity levels higher than 10 mm²/s. A liquid of high viscosity thus possess lower acidity than aqueous or low-viscosity solutions. No significant differences were found between the effects of different thickening agents.

The SBM (stabiliser of biomass) mechanical plant model is designed for the hydrothermal treatment of biologically degradable materials with using the principle of thermal hydrolysis. The primary task of this equipment is to subject biologically degradable materials to hydrothermal treatment and to recover the materials for a further use or to eliminate them. These entities could use the mechanical plant for economic and environment-friendly methods of handling biologically degradable wastes or materials. The objective of the experiment with various types of biologically degradable materials was to establish the level for the transformation of input characteristics of tested materials after conversion in the SBM mechanical plant and to assess the suitability of the processing by using this method with respect to the use of the final product. Materials tested in the first experiments included stable manure heated or unheated during the processing and maize silage wastes in mixture with wood chips.

The efficiency of border rows to prevent the adventitious presence of GM maize in non-GM maize plots was evaluated as well as the effect of the MON 810 maize of the yield and self-protection against the European corn borer. The GM maize MON 810 event was drilled at 3 locations over the Slovakia and the grain samples were collected from the neighbouring conventional maize fields. The data obtained by Real-Time PCR indicate that coexistence between GM and conventional maize is feasible and the isolation distance of 200 m (respectively 100 border rows of conventional maize) separates GM maize from the conventional one more than sufficiently. The effective isolation distance is 3-4 times greater than the actually needed. The MON 810 revealed also the highest yield and the best self-protection against European corn borer in both growing seasons with different climatic conditions.

One of the key goals of the Thematic Strategy for Soil Protection is to maintain and improve soil organic carbon (SOC) stocks. A decline of SOC stocks is politically perceived as a serious threat to soil quality and functions. A suitable tool for acquiring the information on SOC stock changes is modelling. The RothC-26.3 model was applied for long-term modelling (1970-2007) of the SOC stock in the topsoil of croplands of Slovakia. Simulation results show a gradual increase in the SOC stock in the first phase of modelling (1970-1995) mainly due to higher carbon input in the soil. A significant linear correlation (r = 0.4**, n = 275) was found between carbon input and the final simulation of SOC stock. A close relationship between the SOC stock and soil production potential index representing the official basis for soil quality assessment in Slovakia was also determined and a polynomial relationship was found which describes the relation at the 95% confidence level. We have concluded from the results that balanced or positive changes in the SOC stock dynamics that are important for sustainable use of soils could be influenced positively or negatively in Slovakia by political decisions concerning the soil management. Moreover, the soil production potential index can be used as soil quality information support for such decision-making.

The effects of sex and slaughter age on growth, feed intake, carcass composition and meat quality attributes of musculus longissimus lumborum were investigated in Charolais × Simmental bulls (n = 12) and heifers (n = 12) reared and finished under identical management conditions. The animals entered the experiment at similar age (251 days) and were slaughtered at 14 or 18 months of age. Bulls gained more rapidly (P < 0.001), consumed more dry matter daily (P < 0.05), and had a higher killing-out proportion (P < 0.05). The sex × slaughter age interaction was significant (P < 0.01) for feed conversion ratio, which deteriorated markedly more in heifers than in bulls as slaughter age increased. Bulls produced leaner carcasses with a higher proportion of total meat (P < 0.001). While bulls contributed to high-priced meat by a higher proportion of meat from the shoulder (P < 0.01), heifers had higher proportions of meat from the rump and loin (P < 0.05). Older animals were generally fatter and their carcasses contained lower proportions of high-priced meat (P < 0.01) and bones (P < 0.05). Bulls exhibited lower contents of dry matter (P < 0.001), protein (P < 0.05) and intramuscular fat (P < 0.001), and a higher content of collagen (P < 0.001) in musculus longissimus lumborum than hei-fers. The meat from heifers was assessed by the sensory panel as more tender and, when aged for 11 days, more acceptable than the meat from bulls. Older animals obtained higher scores for beef flavour intensity (P < 0.01), tenderness (P < 0.001), juiciness (P < 0.05), and overall acceptance (P < 0.001).

Citrus Greening Disease (CGD) was critically reviewed with respect to its history, origin, symptomatology, host range, pathogenic association, identification and detection, transmission and management. It is evident that the citrus disease has emerged as a potential serious threat to citrus production in all the citrus growing countries of the world including Pakistan. It is has been proved that in Pakistan the greening disease is the major cause of citrus decline; hence it is necessary to formulate definite planning and strategies.

Using a file of economic indicators of a sample of selected agricultural enterprises, their economic results have been evaluated according to their production and climatic conditions, production orientation and the system of management since 1996. The long-term tendencies of economic results and the influencing factors shall be defined according to this analysis. The authors aim to assess the influence of external conditions on management of agricultural enterprises and how the economic result can be influenced. Next, the authors aim to analyse the influence of the accession of the Czech Republic to the EU. In 2005 the agricultural enterprises suffered a decrease in their profit compared with 2004, followed by a decrease of the profit rate. The decrease in the number of labour force and at the same time the increase of labour productivity shows a long-term tendency. Subsidies in agriculture have also been increasing in the long-term perspective. There was the first significant increase in 2004. Subsidies are one of the most important factors influencing the economics of agricultural enterprises and the dependence of their earnings on subsidies is increasing.

The paper presents selected results of the research intent No. MSM 6215648904 "Czech national economy in processes of integration and globalization and the development in sectors of agriculture and services under conditions of European integrated market". Its authors evaluate the effects of subsidies on the development of organic farming in the Czech Republic and in some selected EU countries. In the Czech Republic, the height of subsidies for the organic farming is continuously increasing. In 1998, the total amount of subsidies was 48 million CZK while in 2004 it increased to nearly 277 million CZK. Numbers of organic farms as well as the area under organic farming increased in dependence on the subsidies for this form of agricultural production.

A mathematical sector model has been formulated to optimize the cropping pattern in Saudi Arabia aiming at maximizing the net annual return of the agricultural sector in Saudi Arabia and ensuring the efficient allocation of the scarce water resources and arable land among the competing crops. The results showed the potential for Saudi Arabia to optimize its cropping pattern and to generate an estimated net return equivalent to about 2.42 billion US$ per year. The optimized cropping pattern in Saudi Arabia has been coupled with about 53% saving in the water use and about 48% reduction in the arable land use compared to the base-year cropping pattern. Comparable weights was given to different crop groups by allocating about 48.4%, 35.4%, 13.1%, and 3.2% to grow cereals, fruits, forages, and vegetables, respectively. These findings were in line with the national strategy to rationalize the cultivation of water-intensive crops in favour of highly water-efficient crops.

We tested the hypothesis, suggested by literature data, that in Rumex obtusifolius L. the germination of matured seeds harvested from dry standing shoots and achene morphology are correlated. In these seed materials the level of germination is determined by the incidence of primary seed dormancy. The regression of the percentage of germinating seeds on achene, perianth and seed size and mass were calculated for seed materials of 30 plants. The materials in which achene morphology was investigated were selected ex post from a set of seed materials of 241 plants to cover the range of variation of germinability of individual plants which was between 0-66%. There was no relationship between any characteristic of achene quality and germination except for a positive correlation between germination percentage and achene mass calculated only for seed materials of plants which pro­ duced germinable seeds (i.e. when plants with 0% germination were excluded from the analysis). The second part of the study investigated the effects of size variation on germinability among seeds harvested from one plant and after removal from the perianth. This variation was tested using sets of 20 size-assorted groups of 50 seeds, each originating from a particular plant; materials from six plants were tested. There were significant differences in the average germination between plants. However, in the material from the same plant there was no consistent relationship between seed mass and percentage of germination. We conclude that there exists no direct relationship between achene, perianth or seed size or mass and incidence of primary seed dormancy.

The aim of this study was to estimate potential relationships between insulin-like growth factor I gene polymorphisms (IGF1/TasI and IGF1/SnaBI) and the growth parameters of calves as well as their subsequent milk performance. The study involved a total of 191 Polish Holstein-Friesian var. Black-and-White calves. Both polymorphic sites were determined with Amplification Created Restriction Sites-Polymerase Chain Reaction (ACRS-PCR). In the case of the IGF1/SnaBI polymorphism, the genotype and allele frequencies were as follows: TT - 0.27, CT - 0.55, CC - 0.18, T - 0.54, and C - 0.46. The CC genotype was favourable and associated with higher body weight at the 2^nd month of age of calves (WT60) and their daily body weight gains from the 1^st to the 2^nd month (P ≤ 0.05) as well as subsequent milk, fat, and protein yield (P ≤ 0.01). For the IGF1/TasI polymorphism, the highest frequency of the AA genotype (0.69) was found. Allele frequencies were 0.75 and 0.25 for the A and C alleles, respectively. In the association study, the AC genotype was significantly associated with higher WT60 and average daily gains in the periods from the 2^nd to the 3^rd month of age and for the whole rearing period (P ≤ 0.05) as well as subsequent milk, fat, and protein yield (P ≤ 0.01). Combined genotypes were also included into the analysis. The highest yields of milk as well as fat and protein were found in cows with the CC/AA combination. No clear relationships between body weight and genotype combination were found.

Polymorphisms of DGAT1 and STAT5A genes in Xinong Saanen and Guanzhong goat breeds were investigated. PCR-RFLP, SSCP, and DNA sequencing were used to identify three SNPs: DQ380250:g.407_408insC in the DGAT1 gene, AJ237937:g.6798C>T and g.6852C>T in the STAT5A gene. In DGAT1 g.407_408insC locus, the frequencies of C^- allele were 0.79-0.85, and frequencies of C⁺ allele were 0.21-0.15. At STAT5A g.6852C>T locus, frequencies of C allele were 0.70-0.72, and frequencies of T allele were 0.30-0.28. Compared with goats with DGAT1 C^-C^-, those with C^-C⁺ genotype had greater milk fat (P < 0.05). The goats with STAT5A CT had greater milk yield than those with CC genotype (P < 0.05). The results showed that does with C^-C^-CT and C^-C⁺CT yielded more milk than those with C^-C^-CC(P < 0.05). In addition, does with C^-C⁺CT had the highest milk fat in comparison with other combination genotypes (P < 0.05).